| Project/Area Number |
18580191
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
General fisheries
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| Research Institution | Kitasato University |
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Principal Investigator |
AMANO Masafumi Kitasato University, School of Fisheries Sciences, Associate Prof. (10296428)
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| Co-Investigator(Kenkyū-buntansha) |
TAKAHASHI Akiyoshi Kitasato University, School of Fisheries Sciences, Professor (10183849)
IIGO Masayuki Utsunomiya Univ., Faculty of Agriculture, Associate Prof (10232109)
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| Project Period (FY) |
2006 – 2007
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| Project Status |
Completed (Fiscal Year 2007)
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| Budget Amount *help |
¥3,800,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥300,000)
Fiscal Year 2007: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2006: ¥2,500,000 (Direct Cost: ¥2,500,000)
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| Keywords | Fisheries Science / Physiology / Brain / Feeding / Reproduction / Orexin / GnRH / MCH |
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| Research Abstract |
Orexin/hypocretin is a neuropeptide that is involved in the regulation of feeding behavior and the sleep-wakefulness cycle in mammals. Melanin-concentrating hormone (MCH) is believed to be another candidate involved in food intake in teleost fish as well. The localization of orexin-like immunoreactivity (orexin-LI) in the brain of the medaka was examined. The interaction between the orexin and MCH neurons in the medaka brain was further examined by performing double-staining. immunohistochemistry. Orexin-LI cell bodies were located in the NPPv of the hypothalamus, and orexin-LI fibers were detected throughout the brain. Some orexin-LI fibers were in close contact with the MCH-immunoreactive (it) cell bodies in the hypothalamus. Moreover, a few MCH-ir fibers were in close contact with the orexin-LI cell bodies. These results suggest that in the medaka brain, orexin performs various functions, including neuromodulation, and that neural connections exist between the orexin and MCH neurons
… More
.
Effects of background color on gonadotropin-releasing hormone (GnRH) and MCH levels in the brain of the barfin flounder were monitored to investigate the interaction of GnRH and MCH in the brain. Fish were reared in white or black tanks from one month after hatching for about 7 months. MCH levels in the brain and pituitary were higher in the white tank fish. In contrast, GnRH-II levels in the brain were higher in the black tank fish. Some GnRH-II-ir fibers were in close contact with MCH-ir cell bodies in the hypothalamus. These results indicate that background color affects not only MCH levels but also GnRH-II levels in the brain.
To obtain molecular basis on the roles of HCRT system in teleost, we cloned cDNA encoding HCRT and HCRT receptor (HCRTR) in medaka and their gene structures were clarified. The medaka HCRT cDNA (640 bp) is encoding 139 amino acid residues including HCRT-1 (orexin A) and HCRT-2 (orexin B). The medaka HCRT gene is composed of two exons and one intron. HCRTR is a member of G protein-coupled receptor family with seven transmembrane domains. Two subtypes (HCRTR1 and HCRTR2) of HCRTR were classified to date. However, we found only a single HCRTR gene (HCRTR2) in the medaka genome. The medaka HCRTR2 cDNA (1348 bp) is encoding 420 amino acid residues. The HCRTR2 gene is composed of seven exons and six introns. Less
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