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Immunological analysis of bluefin tuna for establishment of its aquaculture

Research Project

Project/Area Number 18580199
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field General fisheries
Research InstitutionNational Fisheries University

Principal Investigator

INAGAWA Hiroyuki  National Fisheries University, 生物生産学科, 准教授 (30399662)

Co-Investigator(Kenkyū-buntansha) 高橋 幸則  独立行政法人水産大学校, 生物生産学科, 教授 (70399658)
近藤 昌和  独立行政法人水産大学校, 生物生産学科, 講師 (70399666)
澤田 好史  近畿大学, 水産研究所, 准教授 (80319764)
河内 千恵  徳島文理大学, 健康科学研究所, 特別研究員 (00274142)
Project Period (FY) 2006 – 2008
Project Status Completed (Fiscal Year 2008)
Budget Amount *help
¥4,070,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥570,000)
Fiscal Year 2008: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2007: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2006: ¥1,600,000 (Direct Cost: ¥1,600,000)
Keywordsクロマグロ / 免疫 / 養殖 / 腫瘍壊死因子 / TNF
Research Abstract

クロマグロの末梢血白血球の貪食能、リゾチーム活性を明らかにした。また、自然免疫制御に重要な腫瘍壊死因子の遺伝子のクローニングに成功した。予期しなかったことに、クロマグロのTNFはこれまでの魚類では報告されてこなかった別のTNFを有することを初めて見いだした。さらに、これらのTNFの遺伝子組替タンパク質を作製し、白血球の貪食機能を活性化することを見いだした。

Report

(4 results)
  • 2008 Annual Research Report   Final Research Report ( PDF )
  • 2007 Annual Research Report
  • 2006 Annual Research Report
  • Research Products

    (6 results)

All 2008 2006 Other

All Journal Article (2 results) Presentation (4 results)

  • [Journal Article] Two types of tumor necrosis factor-alpha in bluefin tuna (Thunnus orientalis) genes: Molecular cloning and expression profile in response to several immunological stimulants

    • Author(s)
      Kadowaki, T., Harada, H., Morita, H., Sawada, Y., Kohchi, C., Soma, G-I., Takahashi, Y., Inagawa, H.
    • Journal Title

      Fish & Shellfish Immunology (in press)

    • Related Report
      2008 Final Research Report
  • [Journal Article] Two types of tumor necrosis factor-alpha in bluefin tuna (Thunnus orientalis) genes : Molecular cloning and expression profile in response to several immunological stimulants

    • Author(s)
      Kadowaki, T., Harada, H., Morita, H., Sawada, Y., Kohchi, C., Soma, G-I., Takahashi, Y., Inagawa, H.
    • Journal Title

      Fish & Shellfish Immunology (in press, Pubmed 2008, 12. 31)

    • Related Report
      2008 Annual Research Report
  • [Presentation] クロマグロ腫瘍壊死因子(TNF)群の食細胞に対する生物活性2008

    • Author(s)
      門脇健、森田裕久、伊東優太、原田英明、澤田好史、河内千恵、杣源一郎、高橋幸則、稲川裕之
    • Organizer
      平成20年度日本水産学会春季大会
    • Place of Presentation
      清水
    • Related Report
      2008 Final Research Report
  • [Presentation] Cloning and Characterization of Two Types of Tumor Necrosis Factor-α(TNF-α) in Bluefin Tuna, Thunnus orientalis2008

    • Author(s)
      Inagawa, H., Kadowaki, T., Takahashi, Y., Sawada, Y., Kohchi, C., and Soma, G-I.
    • Organizer
      Seventh Symposium on Diseases in Asian Aquaculture
    • Place of Presentation
      Taipei, China
    • Related Report
      2008 Final Research Report
  • [Presentation] Cloning and characterization of two type TNF homologues in bluefin tuna2008

    • Author(s)
      Kadowaki, T., Harada, H., Morita, H., Sawada, Y., Kohchi, C., Soma, G-I., Takahashi, Y., Inagawa, H.
    • Organizer
      5th Wolrld Fisheries Congress
    • Place of Presentation
      Yokohama, Japan
    • Related Report
      2008 Final Research Report
  • [Presentation] クロマグロTNF遺伝子のクローニングと構造及び発現解析の試み2006

    • Author(s)
      原田英明 、安井康眞 、永江彬、迫田美希 、近藤昌和、澤田好史、河内千恵 、杣 源一郎、高橋幸則 、稲川裕之
    • Organizer
      第5回日本水産増殖学会
    • Place of Presentation
      下関
    • Related Report
      2008 Final Research Report

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Published: 2006-04-01   Modified: 2016-04-21  

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