INVESTIGATION OF FISH ROE ALLERGEN AND ITS CROSS-REACTIVITY
| Project/Area Number |
18580200
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Fisheries chemistry
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| Research Institution | Hokkaido University |
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Principal Investigator |
SAEKI Hiroki Hokkaido University, FACULTY OF FISHERIES SCIENCES, Professor (90250505)
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| Co-Investigator(Kenkyū-buntansha) |
HARA Akihiko HOKKAIDO UNIVERSITY, FACULTY OF FISHERIES SCIENCES, PROFESSOR (40091483)
SHIMIZU Yutaka HOKKAIDO UNIVERSITY, FACULTY OF FISHERIES SCIENCES, TECHNICALSTUFF (00374629)
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| Project Period (FY) |
2006 – 2007
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| Project Status |
Completed (Fiscal Year 2007)
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| Budget Amount *help |
¥3,850,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥450,000)
Fiscal Year 2007: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2006: ¥1,900,000 (Direct Cost: ¥1,900,000)
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| Keywords | Food allergy / Fish roe / allergen / vitellogenin / β'-conmponent / IKURA / TARAKO / Yolk protein / 食物アレルギー / サケ |
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| Research Abstract |
Fish roe such as salmon, walleye pollack, and herring roes are popularly consumed in Japan and much cases of fish-roe-allergy, particularly in childhood, have been reported last decade. However; there is little information on fish roe allergens.
The objective of this study is to identify fish roe allergens and to investigate the IgE cross-reactivity among various kinds of fish roes.
The results were as follows: (1) Eleven kinds of fish roes (Churn salmon: Oncorhynchus keta, Sakhalin taimen: Hucho perryi, rainbow trout: Oncorlhynchus mykiss Japanese char: Salvelinus leucomaenis, dolly vuuden: Salvelinus malma, walleye pollack: Theragra chalcogramma, dusky sole Lepidopsetta mochigare, Slime flounder:Microstomus achne, atka mackerel: Plecurogrammus azonus, sishamo smelt: Spirinchus lanceolatus capelin: Mallotus villosus) contained yolk protein that reacted with anti chuun-salmon-β'-component rabbit IgG (anti-β) and they also reacted with specific IgE in chum salmon roe-allergic patients' se
… More
ra.
(2) The major allergens in salmonid fish roes were β'-component and its related fragments.
(3) There were IgE cross-reactivity among salmonid roe β'-components, and the IgE cross--reactivity was also confirmed between churn salmon and walleye pollack roes.
(4) Amino acid sequence of churn salmon β'-component (Mw. 16,000) was examined and the sequences of 91 residues (about 60% of the total sequence) were determined by peptide mapping using the complete sequence of rainbow trout viltellogenin.
(5) cDNA encoding β'-component was obtained using mRNAs extracted firm chum salmon liver and the encoded amino acid sequence was coincided with the sequence obtained by the direct peptide mapping.
(6) β'-component like protein (Mw 17,000) was purified firm walleye pollack roe, and about 70% of the total amino acid sequence was determined using an inside arrangement of Atlantic cod vitellogenin as a template.
These findings would contribute understanding fish roe allergy and improvement of QOL of allergic patients. Less
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Report
(3 results)
Research Products
(15 results)
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[Presentation] Epitopes in IKURA and TARAKO allergen2008
Author(s)
S. Koike, Y Shimizu, K. Watanabe, H. Kishimura, A. Hara, H. Saeki.
Organizer
Annual meeting of The Japanese Society of Fisheries Science
Place of Presentation
University, Shimizu campus
Year and Date
2008-03-31
Description
「研究成果報告書概要(欧文)」より
Related Report
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[Presentation] Detection of common allergen of sahnonid fish roes2007
Author(s)
Y. Shimizu, H. Akashi, A. Nakamura, K. Watanabe, A. Hara, H. Kishimura, H. Saeki
Organizer
Annual meeting of The Japanese Society of Fisheries Science
Place of Presentation
Hokkaido University, Hakodate campus
Year and Date
2007-09-26
Description
「研究成果報告書概要(欧文)」より
Related Report
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