Nitric oxide synthase(NOS) like enzyme mediated nitric oxide(NO) generation by harmful red tide phytoplankton, Chattonella marina
| Project/Area Number |
18580205
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Fisheries chemistry
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| Research Institution | Nagasaki University |
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Principal Investigator |
ODA Tatsuya Nagasaki University, Faculty of Fisheries, Professor (60145307)
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| Co-Investigator(Kenkyū-buntansha) |
YAMAGUCHI Kenichi Nagasaki University, Faculty of Fisheries, Associate Professor (90363473)
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| Project Period (FY) |
2006 – 2007
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| Project Status |
Completed (Fiscal Year 2007)
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| Budget Amount *help |
¥3,830,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥330,000)
Fiscal Year 2007: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2006: ¥2,400,000 (Direct Cost: ¥2,400,000)
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| Keywords | Red tide / phytoplankton / Chattonella marina / reactive oxygen species / nitric oxide / NO synthase / toxicity / flagellate |
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| Research Abstract |
The unicellular marine phytoplankton Chattonella marina is known to exhibit potent fish-killing activity. Previous studies have demonstrated that C. marina produces reactive oxygen species (ROS), and ROS-mediated ichthyotoxic mechanism has been postulated. In fact, several studies have demonstrated that Chattonella spp. generate reactive oxygen species (ROS). Furthermore, our studies using electron spin resonance (ESR) spectroscopy with the spin traps 5,5-dimethyl-1-pyrroline-N-oxide and N-t-butyl-a-phenylnitrone showed that C. marina generates hydroxyl radical (OH). Since ROS are generally considered to be toxic against living organisms, the ROS generated by Chattonella spp. may at least partly involve in the fish-kill mechanism. Furthermore, we have found that C. marina exhibited ROS-mediated toxic effect on a marine bacterium, Vibrio alginolyticus. In addition to Chattonella spp., it has been reported that another raphidophycean flagellate, Heterosigma akashiwo also showed ROS-media
… More
ted toxicity on rainbow trout. Thus, it seems likely that the production of ROS is a common feature of raphidophycean flagellates. However, the exact toxic mechanism is still controversial. In this study, we obtained evidence that C. marina produces nitric oxide (NO)under normal growth conditions. We utilized chemiluminescence (CL)reaction between NO and luminol-H_2O_2 to detect NO in C. marina cell suspensions. In this assay, significant CL was observed in C. marina in a cell-number dependent manner, and this was diminished by the addition of carboxy-PTIO, a specific NO scavenger. The NO generation by C. marina was also confirmed by a spectrophotometric assay based on the measurement of the diazo-reaction positive substances (NO_x)and by fluorometric assay using highly specific fluorescent indicator of NO. The NO level in C. marina was significantly decreased by L-NAME, a specific NO synthase (NOS)inhibitor. The addition of L-arginine resulted in the increase in NO level, whereas NaNO_2 had no effect. These results suggest that a NOS-like enzyme is mainly responsible for NO generation in C. marina. Less
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Report
(3 results)
Research Products
(37 results)
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[Journal Article] Inhibitory or scavenging action of ketoconazole and ciclopiroxolamine against reactive oxygen species released by primed inflammatory cells2007
Author(s)
T., Nakashima, E., Sato, Y., Niwano, M., Kohno, W., Muraoka, T., Oda
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Journal Title
British Journal of Dermatology 156
Pages: 720-727
Description
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[Journal Article] Presence of the distinct systems responsible for superoxide anion and hydrogen peroxide generation in red tide phytoplankton Chattonella marina and Chattonella ovata2007
Author(s)
D., Kim, T., Nakashima, Y., Matsuyama, Y., Niwano, K., Yamaguchi, T., Oda
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Journal Title
J.Plankton Res. 29(3)
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Pages: 427-429
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[Journal Article] Mode of action of an antialgal agent produced by a marine gammaproteobacterium against Chattonella marina2006
Author(s)
T., Nakashima, D., Kim, Y., Miyazaki, K., Yamaguchi, S., Takeshita, T., Oda
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Journal Title
Aquatic Microb.Ecol. 45
Pages: 255-262
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[Journal Article] Producing mechanism of an algicidal compound against red tide phytoplankton in a marine bacterium γ-proteobacterium2006
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Appli.Microbiol.Biotechnol 73
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