| Project/Area Number |
18580208
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Fisheries chemistry
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| Research Institution | Miyagi University |
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Principal Investigator |
NISHIKAWA Masazumi Miyagi University, School of Food, Agricultural and Environmental Sciences, Department of Food Management, Professor (90404839)
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| Co-Investigator(Kenkyū-buntansha) |
HAYAKARI Makoto Hirosaki University School of Medicine, Institute of Brain Science, Professor (80156421)
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| Project Period (FY) |
2006 – 2007
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| Project Status |
Completed (Fiscal Year 2007)
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| Budget Amount *help |
¥3,790,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥390,000)
Fiscal Year 2007: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2006: ¥2,100,000 (Direct Cost: ¥2,100,000)
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| Keywords | Lipids / PUFA / Docosahexaenoic acid / Nutrition / CNS |
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| Research Abstract |
Polyunsaturated fatty acids (PUFA) are occurring in mammals and fishes. Especially, docosahexaenoic acid (DHA) and arachidonic acid (AA) of PUFA are found in the highest concentration in the brain and retina and localized in the 2-position of cell membrane phospholipids and may exert considerable influence on learning ability, visual acuity and Alzheimer's disease. This study was undertaken to find a novel compound among DHA and its derivatives that influences on the functional responses to N-methyl-D-aspartic acid (NMDA) and kainate (KA) in pyramidal neurons acutely isolated from rat cerebral cortex in whole-cell modes under voltage-clamp condition. A total of 27 DHA derivatives were synthesized. DHA potentiated the NMDA response but reduced the non-NMDA (kainate) response in a concentration-dependent manner at a holding potential of -60mV under voltage-clamp condition. AA also potentiated the NMDA response and reduced the KA response in a manner similar to DHA. However, other polyunsaturated and saturated fatty acids had no such effects. No. 2650 and 2657 compounds combined with L-glutamate of the DHA derivatives slightly reduced the NMDA and the KA response. 4-Hydroxy DHA one of the DHA derivatives, reduced the KA response in a manner similar to DHA, but had no effect to the NMDA response. 4-Hydroxy docosahexanoylethanolamine, anandamide analog, slightly potentiated the NMDA and the KA response. However, other DHA derivatives had no such effects. These results indicate that 4-Hydroxy DHA has higher activities than other compounds. Further studies are now needed to prove the potential of this compound and to clarify behavior in vivo.
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