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Construction of the glycerol dehydratase (reuterin-producing) gene knockout mutant Lactobacillus reuteri and probiotics evaluation

Research Project

Project/Area Number 18580275
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Zootechnical science/Grassland science
Research InstitutionAzabu University

Principal Investigator

MORITA Hidetoshi  Azabu University, School of Veterinary Medicine, Associate Professor (70257294)

Co-Investigator(Kenkyū-buntansha) MURAKAMI Masaru  Azabu University, Professor (80271360)
KATO Yukio  Azabu University, Associate Professor (00224551)
Project Period (FY) 2006 – 2007
Project Status Completed (Fiscal Year 2007)
Budget Amount *help
¥3,120,000 (Direct Cost: ¥2,700,000、Indirect Cost: ¥420,000)
Fiscal Year 2007: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2006: ¥1,300,000 (Direct Cost: ¥1,300,000)
KeywordsLactobacillus reuter / reuterin / probiotics / glycerol metabolism / glycerol dehydratase / 遺伝子破壊株 / gup遺伝子 / pduオペロン / モノアソシエイト
Research Abstract

Lactobacillus reuteri is a heterofermentative lactic acid bacterium that naturally inhabits the gut of humans and other animals. The probiotic effects of L. reuterihave been proposed to be largely associated with the production of the broad-spectrum antimicrobial compound reuterin during anaerobic metabolism of glycerol. We determined the complete genome sequences of the reuterin-producing L. reuteri JCM 1112^T. L. reuteriJCM 1112^T has a unique cluster of 58 genes for the biosynthesis of reuterin and cobalamin (vitamin B_<12>). Two-dimensional nuclear magnetic resonance (2D-NMR) with ^<13>G_3-glycerol demonstrated that L. reuteri JCM 1112^T could convert glycerol to reuterin in vivo, substantiating the potential of L. reuteri JCM 1112^T to produce reuterin in the intestine. Given that glycerol is shown to be naturally present in feces, the acquired ability to produce reuterin and cobalamin is an adaptive evolutionary response that likely contributes to the probiotic properties of L. reuteri.

Report

(3 results)
  • 2007 Annual Research Report   Final Research Report Summary
  • 2006 Annual Research Report
  • Research Products

    (47 results)

All 2009 2008 2007 2006 Other

All Journal Article (25 results) (of which Peer Reviewed: 16 results) Presentation (13 results) Book (2 results) Patent(Industrial Property Rights) (7 results)

  • [Journal Article] Lactobacillus hayakitensis, L. equigeneroai and L. equi, predominant lactobacilli of the intestinal flora of healty Thoroubreds2009

    • Author(s)
      Morita, H., Nakano, A., et al.
    • Journal Title

      Anim. Sci. J. 80(In press)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
    • Peer Reviewed
  • [Journal Article] Comparative genome analysis of Lactobacillus reuteri and Lactobacillus fermentum reveal a genomic island for reuterin and cobalamin production2008

    • Author(s)
      Morita, H., Toh, H., Fukuda, S., Horikawa, H., Oshima, K., Hattori, M., et al.
    • Journal Title

      DNA Res. 15

      Pages: 151-161

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
    • Peer Reviewed
  • [Journal Article] Comparative genome analysis of Lactobacillus reuteri and Lactobacillus fermentum reveal a genomic island for reuterin and cobalamin production2008

    • Author(s)
      Morita, H., Toh, H., Fukuda, S., Horikawa, H., Oshima, K. Hattori, M., et al.
    • Journal Title

      DNA Res. 15

      Pages: 151-161

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] Comparative genome analysis of Lactobacillus reuteri and Lactobacillus fermentum reveal a genomic island for reuterin and cobalamin production2008

    • Author(s)
      Morita, H., 他18名(1st name, correspondence)
    • Journal Title

      DNA Res. 15(In press)

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Sharpea azabuensis gen. nov., sp. nov., a Gram-positive strict anaerobic bacterium isolated from the faeces of Thoroughbred horses2008

    • Author(s)
      Morita, H., 他10名(1st name, correspondence)
    • Journal Title

      Int. J. Syst. Evol. Microbiol. 58(In press)

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Cell wall fraction of Enterococcus hirae ameliorates TNF-α-induced barrier impairment in the human epithelial tight junction2008

    • Author(s)
      Morita, H., 他5名(2nd name)
    • Journal Title

      Lett. Appl. Microbiol. 46(In press)

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Journal Article] The effect of oral administration of 3,3',4,4',5-pentachlorobiphyenl(PCB126)on the intestinal microbiota of Sprague-Dawley rats2008

    • Author(s)
      Morita, H., 他7名(last name, correspondence)
    • Journal Title

      Anim. Sci. J. 79

      Pages: 391-400

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Lactobacillus equigenerosi sp. nov., a coccoid species isolated from faeces of thoroughbred racehorses2008

    • Author(s)
      Morita, H, 他4名(4th name)
    • Journal Title

      Int. J. Syst. Evol. Miocrbiol. 58

      Pages: 914-918

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Lactobacillus equigenerosi sp. nov., a coccoid species isolated from faeces of thoroughbred racehorses2008

    • Author(s)
      Morita, H., 他2名(last name)
    • Journal Title

      Int. J. Syst. Evol. Miocrbiol. 58

      Pages: 914-918

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Toll-like receptor (TLR)2 induced through TLR4 signaling initiated by Helicoba cter pylori cooperatively amplifies iNOS induction in gastric epithelial cells2007

    • Author(s)
      Uno, K, Kato, K, Morita, H., Yoshimura, T., et al.
    • Journal Title

      Am. J. Physiol : Gastrointestinal and Liver Physiol. 293

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
    • Peer Reviewed
  • [Journal Article] Toll-like receptor (TLR) 2 induced through TLR4 signaling initiated by Helicobacter pyloriCooperatively amplifies iNOS induction in gastric epithelial cells2007

    • Author(s)
      Uno, K., Kato, K., Morita, H., Yoshimura, T., et al.
    • Journal Title

      Am. J. Physiol. : Gastrointestinal and Liver Physiol. 293

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] Health function and application of lactic acid bacteria2007

    • Author(s)
      Morita, H., Toh, H.
    • Journal Title

      CMC Press

      Pages: 95-106

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] Toll-like receptor(TLR)2 induced through TLR4 signaling initiated by Helicobacter pylori cooperatively amplifies iNOS inductiion in gastric epithelial cells2007

    • Author(s)
      Morita, H., 他9名(6th name)
    • Journal Title

      Am. J. Physiol.: Gastrointestinal and Liver Physiol. 293

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Comparative metagenomics revealed commonly enriched gene sets in human gut microbiomes2007

    • Author(s)
      Morita, H., 他19名(8th name)
    • Journal Title

      DNA Res. 14

      Pages: 169-181

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Lactobacillus hayakitensis sp. nov., isolated from intestines of healthy Thoroughbred2007

    • Author(s)
      Morita, H., 他9名(1st name, correspondence)
    • Journal Title

      Int. J. Syst. Evol. Miocrbiol. 57

      Pages: 2836-2839

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Aqueous extracts of Rhizopus oryzae induce apoptosis in human promyelocytic leukemia cell line HL-602007

    • Author(s)
      Morita, H., 他3名(3rd name)
    • Journal Title

      J. Health Sci. 53

      Pages: 760-765

    • NAID

      110006545946

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Journal Article] An improved DNA isolation method for metagenomic analysis of the microbial flora of the human intestine2007

    • Author(s)
      Morita, H., 他7名(1st name)
    • Journal Title

      Microbes Environ. 22

      Pages: 214-222

    • NAID

      110006379878

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Potency of water extracts of Rhizopus oryzae on the Salmonella infection rat2007

    • Author(s)
      Morita, H., 他3名(last name, correspondence)
    • Journal Title

      Food Sci. Technol. Res. 13

      Pages: 235-240

    • NAID

      10019859057

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Compositions of Lactobacillus, Enterococcus, and Bifidobacteriumin feces of active racing horses, as determined by PCR-denaturing gradient gel electrophoresis and real-time PCR using group-specific primers2007

    • Author(s)
      Morita, H., 他4名(4th name)
    • Journal Title

      J. Gen. Appl. Microbiol. 53

      Pages: 191-200

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Journal Article] 乳酸菌のゲノム解析の進展(総説)2007

    • Author(s)
      森田英利, 他1名(1st name, correspondence)
    • Journal Title

      腸内細菌学会誌 21

      Pages: 209-215

    • Related Report
      2007 Annual Research Report
  • [Journal Article] Molecular monitoring of the main changes in bacterial floral diversity in the gastrointestinal tract of a thoroughbred foal with catarrhal enteritis by using PCR-DGGE2007

    • Author(s)
      Morita, H.(1st name, correspondence)他9名
    • Journal Title

      J. Equine Vet. Sci. 27

      Pages: 14-19

    • Related Report
      2006 Annual Research Report
  • [Journal Article] Characterization of Lactococcus garvieae isolated from radish and broccoli sprouts that exhibited a KG^+ phenotype, lack of virulence and absence of a capsule2007

    • Author(s)
      Morita, H.(last name)他9名
    • Journal Title

      Lett. Appl. Microbiol. 102(In press)

    • NAID

      120000876455

    • Related Report
      2006 Annual Research Report
  • [Journal Article] Differences between Lactococcus garvieae isolated from the genus Seriola in Japan and those isolated from other animals (trout,terrestrial animals from Europe)with regard to pathogenicity, phage susceptibility and genetic characterization2006

    • Author(s)
      Morita, H.(8th name) 他8名
    • Journal Title

      J. Appl. Microbiol. 101

      Pages: 496-504

    • Related Report
      2006 Annual Research Report
  • [Journal Article] The formation of g=2.49-species of cytochrome P450 in the rat liver by PCB126 oral administration : Identification of heme axial ligands by EPR spectroscopy2006

    • Author(s)
      Morita, H.(1st name, correspondence)他5名
    • Journal Title

      Biosci. Biotech. Biochem. 70

      Pages: 2974-2981

    • NAID

      10018523952

    • Related Report
      2006 Annual Research Report
  • [Journal Article] Lactobacillus hayakitensis, L. equigenerosi and L. equi, predominant lactobacilli of the intestinal flora of healty Thoroubreds

    • Author(s)
      Morita, H., Nakano, A., et al.
    • Journal Title

      Anim. Sci. J. 80 (in press)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] 全ゲノム情報を用いたヒト消化管由来Lactobacillus reuteriの種々の機能性に関する科学的証明2007

    • Author(s)
      森田英利
    • Organizer
      平成19年度(財)神奈川科学技術アカデミー研究報告会
    • Place of Presentation
      川崎市・かながわサイエンスパーク
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] ゲノム情報からのLactobacillus reuteri研究2007

    • Author(s)
      森田英利
    • Organizer
      (独)酒類総合研究所NRIB特別セミナー
    • Place of Presentation
      東広島市・酒類総合研究所
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Annual Research Report 2007 Final Research Report Summary
  • [Presentation] Scientific probiotic evidence of Lactobacillus reuteri complete genome2007

    • Author(s)
      Morita, H.
    • Organizer
      Congress of Scientific Academy of Kanagawa
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] Study on Lactobacillus reuteri complete genome2007

    • Author(s)
      Morita, H.
    • Organizer
      NRIB Seminar
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] Helicobacter pyloriこよるToll-like receptor(TLR)4シグナル系を介したTLR2発現増強と胃粘膜上皮細胞におけるiNOS誘導への協働作用について2007

    • Author(s)
      森田英利, 他7名(5th name)
    • Organizer
      第29回日本フリーラジカル学会/日本過酸化脂質・フリーラジカル学会第31回大会合同学会
    • Place of Presentation
      名古屋国際会議場
    • Related Report
      2007 Annual Research Report
  • [Presentation] Lactobacillus reuteriの産生する3-ヒドロキシプロピオンアルデヒドのin vivo検出2007

    • Author(s)
      森田英利, 他10名(演者)
    • Organizer
      2007年度 日本乳酸菌学会大会
    • Place of Presentation
      相模原市・麻布大学
    • Related Report
      2007 Annual Research Report
  • [Presentation] サラブレッドの消化管から分離されたLactobacillus属23菌種の系統解析2007

    • Author(s)
      森田英利, 他6名(演者)
    • Organizer
      日本畜産学会第108回大会
    • Place of Presentation
      岡山市・岡山大学
    • Related Report
      2007 Annual Research Report
  • [Presentation] サラブレッドの消化管から分離したLactobacillus属とBifidobacterium属のサラブレッドへの投与効果2007

    • Author(s)
      森田英利, 他8名(演者)
    • Organizer
      日本ウマ科学会第20回学術集会
    • Place of Presentation
      東京都文京区・東京大学弥生講堂
    • Related Report
      2007 Annual Research Report
  • [Presentation] Lactic acid bacterial lipoteichoic acid reverses TNF-α-induced barrier impairment in human epithelial tight junction2007

    • Author(s)
      Morita, H., 他5名(5th name)
    • Organizer
      International Conference on Food Factors for Health Promotion(ICoFF2007)
    • Place of Presentation
      京都市・国際会議場
    • Related Report
      2007 Annual Research Report
  • [Presentation] Complete genome sequences of fish-pathogenic and non-pathogenic strains of Lactococcus garvieae isolated from Seriola2007

    • Author(s)
      森田英利, 他7名(演者)
    • Organizer
      The 7th Workshop on Advanced Genomics: New Horizon
    • Place of Presentation
      Tokyo International Forum Hall B7
    • Related Report
      2007 Annual Research Report
  • [Presentation] サラブレッドの消化管由来Lactobacillus属細菌23菌種の系統解析2007

    • Author(s)
      森田英利, 他9名(演者)
    • Organizer
      2007年度 日本分子生物学会年会
    • Place of Presentation
      横浜市・パシフィコ横浜
    • Related Report
      2007 Annual Research Report
  • [Presentation] ヒト腸内常在大腸菌のゲノム解析2007

    • Author(s)
      森田英利, 他9名(6th name)
    • Organizer
      2007年度 日本分子生物学会年会
    • Place of Presentation
      横浜市・パシフィコ横浜
    • Related Report
      2007 Annual Research Report
  • [Presentation] プロバイオティクス細菌Lactobacillus reuteriの抗菌物質生産のin vivo証明と比較ゲノム解析2007

    • Author(s)
      森田英利, 他7名(演者)
    • Organizer
      第1回日本ゲノム微生物学会年会
    • Place of Presentation
      木更津市・かずさアカデミアホール
    • Related Report
      2007 Annual Research Report
  • [Book] 乳酸菌の保健機能と応用2007

    • Author(s)
      森田英利, 藤 英博
    • Total Pages
      12
    • Publisher
      (株)シーエムシー出版
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Book] 乳酸菌の保健機能と応用2007

    • Author(s)
      森田英利・藤 英博
    • Total Pages
      17
    • Publisher
      (株)シーエムシー出版
    • Related Report
      2007 Annual Research Report
  • [Patent(Industrial Property Rights)] 馬由来のビフィドバクテリウム・ボームを有効成分として含有することを特徴とする馬用乳酸菌製剤2007

    • Inventor(s)
      森田英利, 他1名
    • Industrial Property Rights Holder
      森田英利, 他1名
    • Industrial Property Number
      2007-030609
    • Filing Date
      2007
    • Related Report
      2007 Annual Research Report
  • [Patent(Industrial Property Rights)] 新規なラクトバチルス属微生物および馬用乳酸菌製剤先の出願に基づく優先権主張新規なラクトバチルス属微生物および乳酸菌製剤2007

    • Inventor(s)
      森田英利
    • Industrial Property Rights Holder
      森田英利, 他1名
    • Industrial Property Number
      2007-030608
    • Filing Date
      2007
    • Related Report
      2007 Annual Research Report
  • [Patent(Industrial Property Rights)] 新規なラクトバチルス属微生物および馬用乳酸菌製剤先の出願に基づく優先権主張新規なラクトバチルス属微生物および乳酸菌製剤2007

    • Inventor(s)
      森田英利
    • Industrial Property Rights Holder
      森田英利, 他1名
    • Industrial Property Number
      2007-269758
    • Filing Date
      2007
    • Related Report
      2007 Annual Research Report
  • [Patent(Industrial Property Rights)] 新規なラクトバチルス属微生物および哺乳動物用乳酸菌製剤2007

    • Inventor(s)
      森田英利
    • Industrial Property Rights Holder
      森田英利, 他1名
    • Industrial Property Number
      2007-276415
    • Filing Date
      2007
    • Related Report
      2007 Annual Research Report
  • [Patent(Industrial Property Rights)] 病原性ラクトコッカス・ガルビエの検出方法2007

    • Inventor(s)
      森田英利
    • Industrial Property Rights Holder
      森田英利, 他1名
    • Industrial Property Number
      2007-304117
    • Filing Date
      2007
    • Related Report
      2007 Annual Research Report
  • [Patent(Industrial Property Rights)] 免疫調節剤および免疫調節食品2006

    • Inventor(s)
      森田英利, 他
    • Industrial Property Rights Holder
      森田英利, 他
    • Industrial Property Number
      2006-037371
    • Filing Date
      2006
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Patent(Industrial Property Rights)] 免疫調節剤および免疫調節食品2006

    • Inventor(s)
      森田英利・他
    • Industrial Property Rights Holder
      丸大食品・森田英利
    • Industrial Property Number
      2006-037371
    • Filing Date
      2006-12-05
    • Related Report
      2006 Annual Research Report

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Published: 2006-04-01   Modified: 2016-04-21  

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