| Project/Area Number |
18580278
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Applied animal science
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| Research Institution | Tokyo University of Agriculture and Technology |
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Principal Investigator |
SATO Kan Tokyo University of Agriculture and Technology, Applied Bioproduction Science, Associate professor (20250730)
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| Project Period (FY) |
2006 – 2007
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| Project Status |
Completed (Fiscal Year 2007)
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| Budget Amount *help |
¥3,830,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥330,000)
Fiscal Year 2007: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2006: ¥2,400,000 (Direct Cost: ¥2,400,000)
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| Keywords | Chicken / adipocyte / Transcriptional factors / Ligand / Promoter / PPARγ |
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| Research Abstract |
The aim of the present study was to investigate identification of novel fatty acid dependent transcriptional signaling pathway during chicken adipogenesis and to develop the possible procedure for production of low fat meat using modification of the gene expression and regulation of cell proliferation in post-hatched chicks.
(1) The analysis of transcriptional factors involved in the stimulation of PPARγ gene expression during fatty acid induced chicken adipogenesis
Preadipocyte cells cultured in differentiation media containing 200-300 μM oleate and hormones showed a marked increase in lipid deposition in relation to the control cultured in growth medium. Rapid increases in PPARγ and aP2 expression were observed after 9 and 12 h of culture in differentiation medium, respectively. However, the rapid adipocyte differentiation was no induced by the supplementation of PPARγ and PPARβ/δ agonists, i.e. troglitazone and GW501516, in fatty acid free differentiation media. Then, it is suggested
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that PPARγ is possibly a key regulator in the early stages of chicken preadipocyte differentiation there are fatty acid responsible enhancer elements in PPARγ promoter.
(2) Promoter assay of chicken PPARγ on fatty acid induced adipogenesis
The suppresser and enhancer of fatty acid responsible elements were identified in chicken PPARγ promoter at -700- -900 and -1000- -2000, respectively. The predictive transcriptional factors of fatty acid responsible enhancer elements were RSRFC4, CREB, or HDF-2.
(3) Possible procedure for production of low fat meat using modification of the gene expression and regulation of cell proliferation in post-hatched chicks
At 48 days of age, adipose fat pad weight and feed intake were significantly decreased in chickens given a single intraperitoneal dose of troglitazone at one-day old compared to controls, although body weight and carcass composition were not different between troglitazone-treated and control chickens. These results demonstrate that a single intraperitoneal injection of troglitazone to newly hatched chicks reduces fat deposition in mature broiler chickens. Less
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