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Protein tyrosine phosphorylation in boar sperm capacitation induced by a novel relaxin-like protein

Research Project

Project/Area Number 18580280
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Applied animal science
Research InstitutionShizuoka University

Principal Investigator

KOHSAKA Tetsuya  Shizuoka University, Faculty of Agriculture, Professor (10186611)

Project Period (FY) 2006 – 2007
Project Status Completed (Fiscal Year 2007)
Budget Amount *help
¥4,010,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥510,000)
Fiscal Year 2007: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
Fiscal Year 2006: ¥1,800,000 (Direct Cost: ¥1,800,000)
KeywordsRelaxin / Testis / Sperm / Capacitation / Phosphorvlation / Boar
Research Abstract

This research project was planed to elucidate protein tyrosine phosphorylation during boar sperm capacitation and acrosome reaction mediated by relaxin-like protein (seminal relaxin). The outcome of this study as follows.
The present study demonstrated that seminal relaxin actually induced boar sperm capacitation and acrosome reaction with high sperm viability. In relaxin-treated boar sperm, the activity of protein tyrosine kinase was increased with progress of the incubation time, which reached a plateau (10U/10^8 cells) at 4 h of incubation. Furthermore, Western blot analysis revealed a specific band of phosphorylated protein corresponding to the molecular size of 30 kDa in the capacitated/acrosome reacted boar sperm induced by relaxin, suggesting that relaxin induces tyrosine phosphorylation in boar sperm. Also proteome analysis characterized it as interesting and unusual. When polyclonal antibody was generated to the recombinant phosphorylated protein-MBP fusion protein expressed in E. coli, immunohistochemical analysis provided clear-cut evidence of the localization of phosphorylated protein in the sperm head and midpiece.

Report

(3 results)
  • 2007 Annual Research Report   Final Research Report Summary
  • 2006 Annual Research Report
  • Research Products

    (19 results)

All 2008 2007 2006

All Journal Article (15 results) (of which Peer Reviewed: 4 results) Presentation (2 results) Book (2 results)

  • [Journal Article] Effect of relaxin and IGF-1 on capacitation, acrosome reaction, cholesterol efflux and utilization of labeled and unlabeled glucose in porcine spermatozoa2008

    • Author(s)
      AG Miah, et. al.
    • Journal Title

      Reproductive Medicine and Biology 7

      Pages: 29-36

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
    • Peer Reviewed
  • [Journal Article] Effect of relaxin and IGF-I on capacitation, acrosome reaction, cholesterol efflux and utilization of labeled and unlabeled glucose in porcine spermatozoa2008

    • Author(s)
      Miah, AG., Salma, U., Takagi, Y., Kohsaka, T., Hamano, K., Tsuji, H
    • Journal Title

      Reproductive Medicine and Biology 7

      Pages: 29-36

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] Expression and cellular pattern of relaxin mRNA in porcine corpora lutea during pregnancy2007

    • Author(s)
      T. Kohsaka, et. al.
    • Journal Title

      Cell and Tissue Research 330

      Pages: 303-312

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
    • Peer Reviewed
  • [Journal Article] Expression and cellular pattern of relaxin mRNA in porcine corpora lutea during pregnancy2007

    • Author(s)
      Kohsaka, T., Singh, UP., Yogo, K., Sasada, H., Taya, K., Hashizume, K
    • Journal Title

      Cell and Tissue Research 330

      Pages: 303-312

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] Effect of relaxin on the motility, acrosome reaction and utilization of glucose of fresh and frozen-thawed bovine spermatozoa2007

    • Author(s)
      Miah, AG., Salma, U., Tareq, K., Kohsaka, T., Tsuji, H
    • Journal Title

      Animal Science Journal 78

      Pages: 495-502

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] Expression and cellular pattern of relaxin mRNA in porcine corpora lutea during pregnancy2007

    • Author(s)
      T.Kohsaka, et. al.
    • Journal Title

      Cell and Tissue Research 330

      Pages: 303-312

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Effect of relaxin on the motility,acrosome reaction and utilization of glucose of fresh and frozen-thawed bovine spermatozoa2007

    • Author(s)
      A.Miah, et. al.
    • Journal Title

      Animal Science Journal 78

      Pages: 495-502

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Identification of relaxin and its receptor LGR7 in the boar testis.2006

    • Author(s)
      K. Maruyama, et. al.
    • Journal Title

      Proceedings of 12th AAAP Animal Science Congress XII

      Pages: 1-3

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] Effect of relaxin on in vitro fertilization of porcine oocytes2006

    • Author(s)
      Young-Joon, H., Miah, AG., Yoshida, M., Sasada, H., Hamano, K., Kohsaka, T
    • Journal Title

      Journal of Reproduction and Development 52

      Pages: 657-662

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] Identification of relaxin and its receptor LGR7 in the boar testis2006

    • Author(s)
      Maroyama, K., Kato, S., Aoshima, T., Suchin, Itoh, H., Yogo, K., Kawarasaki, T., Yamada, K., Kitayama, Y., Kohsaka, T
    • Journal Title

      Proceedings of 12th AAAP Animal Science Congress XII

      Pages: 1-3

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] Effect of relaxin on motility, acrosome reaction and viability of cryopreserved boar spermatozoa2006

    • Author(s)
      Miah, AG., Sharoare, MD., Tareq, KM., Salma, U., Hamano, K., Kohsaka, T
    • Journal Title

      Reproductive Medicine and Biology 5

      Pages: 215-220

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] Effect of relaxin on acrosome reaction and utilization of glucose in boar spermatozoa2006

    • Author(s)
      Miah, AG., Tareq, KM., Hamano, K., Kohsaka, T., Tsuji, H
    • Journal Title

      Journal of Reproduction and Development 52

      Pages: 773-779

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] Park EY Identification of epitope on DNA-binding protein expressed in insect cell infected by baculovirus2006

    • Author(s)
      Suzuki, M., Kohsaka, T
    • Journal Title

      Molecular Biology Reports 33

      Pages: 97-102

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] Identification of relaxin and its receptor LGR7 in the boar testis2006

    • Author(s)
      Maroyama et al.
    • Journal Title

      Proceedings of XIIth AAAP Animal Science Congress 12

      Pages: 521-524

    • Related Report
      2006 Annual Research Report
  • [Journal Article] Effect of relaxin on motility, acrosome reaction and viability of cryopreserved boar spermatozoa2006

    • Author(s)
      Miah A et al.
    • Journal Title

      Reproductive Medicine and Biology 5

      Pages: 215-220

    • Related Report
      2006 Annual Research Report
  • [Presentation] Identification of relaxin and its receptor LGR7 in the boar testis2006

    • Author(s)
      K. Maruyama, et. al.
    • Organizer
      XIIth AAAP Animal Science Congress
    • Place of Presentation
      韓国釜山
    • Year and Date
      2006-09-21
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] Identification of relaxin and its receptor LGR7 in the boar testis.2006

    • Author(s)
      K. Maruyama, S. Kato, T. Aoshima, Suchin, H. Itoh, K. Yogo, T. Kawarasaki, K. Yamada, Y. Kitayam, T. Kohsaka
    • Organizer
      XIIth AAAP Animal Science Congress
    • Place of Presentation
      Korea
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Book] ホルモンハンドブック 新訂eBook版(日本比較内分泌学会編)2007

    • Author(s)
      高坂哲也(分担執筆)
    • Total Pages
      2464
    • Publisher
      南江堂
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Book] ホルモンハンドブック新訂eBook版(日本比較内分泌学会編)2007

    • Author(s)
      高坂 哲也(分担執筆)
    • Total Pages
      2464
    • Publisher
      南江堂
    • Related Report
      2007 Annual Research Report

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Published: 2006-04-01   Modified: 2016-04-21  

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