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Role of ABC transporter Bcrp 1 in maintenance of pluripotency in mouse embryonic stem cells

Research Project

Project/Area Number 18580283
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Applied animal science
Research InstitutionKinki University

Principal Investigator

MITANI Tasuku  Kinki University, Institute of Advanced technology, Associate Professor (10322265)

Co-Investigator(Kenkyū-buntansha) SAEKI Kazuhiro  Kinki University, School of Biology-Oriented Science and Technology, Professor (10298937)
MATSUMOTO Kazuya  Kinki University, School of Biology-Oriented Science and Technology, Professor (20298938)
TAGUCHI Yoshitomo  Kinki University, School of Biology-Oriented Science and Technology, Lecturer (70309269)
Project Period (FY) 2006 – 2007
Project Status Completed (Fiscal Year 2007)
Budget Amount *help
¥3,290,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥390,000)
Fiscal Year 2007: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2006: ¥1,600,000 (Direct Cost: ¥1,600,000)
KeywordsABC transporter / Bcrp 1 / ES cells / Oct3 / 4 / Flowcytometry / Differentiation / isoform / Bcrp1 / リアルタイムPCR / 胚性幹細胞 / Oct4
Research Abstract

In this research, we aimed to define the role of ABC transporter Bcrpl in the mechanisms of maintenance of pluripotency in mouse embryonic stem cells. Expression profile of Bcrpl protein and mRNA in undifferentiated and differentiated ES cells was examined by flowcytometry and RTP-CR analysis. Moreover, we developed modified dual luciferase reporter assay system to evaluate RNAi efficacy.
1. Correlation of the expression of Bcrpl with Oct3/4 in mouse ES cells.
Flowcytometry analysis showed the expression of Bcrpl positively correlated with Oct3/4, a transcription factor regulating undifferentiated status, in mouse ES cells. Moreover, the expression of Bcrpl as well as Oct3/4 in ES cells decreased after in intro differentiation.
2. Expression of Bcrpl mRNA isoforms in ES cells.
Mouse Bcrpl mRNA has three isoforms (termed isoform A, B and C) and the differential expression of Bcrpl mRNA isoforms is alternatively regulated in the stem cells and somatic cells. The expression pattern of Bcrpl mRNA isoforms in various tissues in adult mice and undifferentiated ES cells was analyzed by RT-PCR. The transcript of isoform B was strongly expressed in all tissues and ES cells. The isoform A was also expressed in the most tissues and ES cells but isoform C was alternatively transcribed and was quite low in ES cells. After induction of in vitro differentiation, all Bcrpl mRNA isoforms immediately decreased and various differentiation markers appeared thereafter.
3. Development of modified dual luciferase reporter assay system to evaluate RNAi efficacy.
In order to establish knockdown ES cell lines against each isoforms of Bcrpl mRNA for functional analysis, we developed modified dual luciferase reporter assay system to evaluate RNAi efficacy. Reporter-based luciferase assay system represented positive correlation with Western blot analysis in the reduction of target protein by shRNA expression vector in mouse ES cells.

Report

(3 results)
  • 2007 Annual Research Report   Final Research Report Summary
  • 2006 Annual Research Report
  • Research Products

    (114 results)

All 2008 2007 2006 Other

All Journal Article (40 results) (of which Peer Reviewed: 16 results) Presentation (70 results) Remarks (2 results) Patent(Industrial Property Rights) (2 results)

  • [Journal Article] Expression of transcription factors specific to the trophoblast lineage in mouse somatic nuclear transfer embryos2008

    • Author(s)
      Mitani T, et. al.
    • Journal Title

      Reprod.Fert.Dev. 20

      Pages: 103-104

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
    • Peer Reviewed
  • [Journal Article] Cis-acting elements (E-box and NBE) in the promoter region of three maternal genes (Histone Hloo,Nucleoplasmin 2,and Zygote Arrest 1) are required for oocyte-specific gene expression in the mouse2008

    • Author(s)
      Tsunemoto K, et. al.
    • Journal Title

      Mol.Reprod.Dev. March

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
    • Peer Reviewed
  • [Journal Article] Identification of ZAG1,a novel protein expressed in mouse preimplantation,and its putative roles in zygotic activation2008

    • Author(s)
      Matsuoka T, et. al.
    • Journal Title

      J.Reprod.Dev. March

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
    • Peer Reviewed
  • [Journal Article] マウス胚性幹細胞におけるBcrp1 mRNAアイソフォームの発現2008

    • Author(s)
      川村 紘子, 他
    • Journal Title

      近畿大学先端技術総合研究所紀要 13

      Pages: 29-40

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] ウシ雄性生殖細胞におけるゴノサイトおよび精原幹細胞の同定2008

    • Author(s)
      森木 甲子郎, 他
    • Journal Title

      近畿大学先端技術総合研究所紀要 13

      Pages: 41-50

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Annual Research Report 2007 Final Research Report Summary
  • [Journal Article] 体細胞由来クローンマウスの成育および生殖能力の検討とマイクロサテライトマーカーによる遺伝的背景の解析2008

    • Author(s)
      森田 真裕, 他
    • Journal Title

      近畿大学先端技術総合研究所紀要 13

      Pages: 51-59

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Annual Research Report 2007 Final Research Report Summary
  • [Journal Article] Expression of transcription factors specific to the trophoblast lineage in mouse somatic nuclear transfer embryos.2008

    • Author(s)
      Mitani T, et. al.
    • Journal Title

      Reprod. Fert. Dev. 20

      Pages: 103-104

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] Cis-acting elements(E-box and NBE) in the promoter region of three maternal genes(Histone H1oo, Nucleoplasmin 2, and Zygote Arrest 1) are required for oocyte-specific gene expression in the mouse.2008

    • Author(s)
      Tsunemoto K, et. al.
    • Journal Title

      Mol. Reprod. Dev. (Publised on line)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] Identification of ZAG1, a novel protein expressed in mouse preimplantation, roles in zygotic activation.2008

    • Author(s)
      Matsuoka T, et. al.
    • Journal Title

      J. Reprod. Dev. and its putative (Publised on line)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] Expression of Bcrp1 mRNA isoforms of mouse embryonic stem cells2008

    • Author(s)
      Kawamura H, et. al.
    • Journal Title

      Mem. Inst. Adv. Technol., Kinki Univ. 13

      Pages: 29-40

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] Expression of stem and germ cell markers in juvenile bovine testis and its primary culture2008

    • Author(s)
      Moriki K, et. al.
    • Journal Title

      Mem. Inst. Adv. Technol., Kinki Univ. 13

      Pages: 41-50

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] Growth and fertility of cloned mouse by somatic cell nuclear transfer and microsatellite analysis of its genetic background2008

    • Author(s)
      Morita M, et. al.
    • Journal Title

      Mem. Inst. Adv. Technol., Kinki Univ. 13

      Pages: 51-59

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] Identification of ZAG1,a novel protein expressed in mousepreimplantation,and its putative roles in zygotic activation2008

    • Author(s)
      Matsuoka T, et. al.
    • Journal Title

      J.Reprod.Dev 54

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Expression oftranscription factors specific to the trophoblast lineagein mouse somatic nuclear transfer embryos2008

    • Author(s)
      Mitani T, et. al.
    • Journal Title

      Reprod.Fert.Dev 20

      Pages: 103-104

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Journal Article] マウス胚性幹細胞におけるBcrp1mRNAアイソフォームの発現2008

    • Author(s)
      川村 紘子, 他
    • Journal Title

      近畿大学先端技術総合研究所紀要 13

    • Related Report
      2007 Annual Research Report
  • [Journal Article] Transgenic siRNA increases the disease duration of ALS in a mouse model2007

    • Author(s)
      Yokota T, et. al.
    • Journal Title

      Archives Neurol. 64

      Pages: 145-146

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
    • Peer Reviewed
  • [Journal Article] Differentiation of hepatocyte-like cells from mouse embryonic stem cells in a monolayer culture system2007

    • Author(s)
      Mitani T, et. al.
    • Journal Title

      Reprod.Fert.Dev. 19

      Pages: 230-230

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
    • Peer Reviewed
  • [Journal Article] Abnormal distribution of chromosomes in first division of nuclear transferred mouse embryos2007

    • Author(s)
      Kawasumi M, et. al.
    • Journal Title

      J.Reprod.Dev. 53

      Pages: 615-622

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
    • Peer Reviewed
  • [Journal Article] Primate embryonic stem cells proceed to early gametogenesis in vitro2007

    • Author(s)
      Teramura T, et. al.
    • Journal Title

      Cloning Stem Cells 9

      Pages: 144-156

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Annual Research Report 2007 Final Research Report Summary
    • Peer Reviewed
  • [Journal Article] H1foo is indispensable for meiotic maturation of the mouse oocyte2007

    • Author(s)
      Furuya M, et. al.
    • Journal Title

      J.Reprod.Dev. 53

      Pages: 895-902

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
    • Peer Reviewed
  • [Journal Article] A mouse and embryonic stem cells derived from a single embryo2007

    • Author(s)
      Teramura T, et. al.
    • Journal Title

      Cloning Stem Cells 9

      Pages: 485-494

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
    • Peer Reviewed
  • [Journal Article] マウス体細胞核移植胚における転写因子Cdx2ならびにOct3/4の発現解析2007

    • Author(s)
      西脇 恵, 他
    • Journal Title

      近畿大学先端技術総合研究所紀要 12

      Pages: 33-42

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] Transgenic siRNA increases the disease duration of ALS in a mouse model.2007

    • Author(s)
      Yokota T, et. al.
    • Journal Title

      Archives Neurol. 64

      Pages: 145-146

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] Differentiation of hepatocyte-like cells from mouse embryonic stem cells in a monolayer culture system.2007

    • Author(s)
      Mitani T, et. al.
    • Journal Title

      Reprod. Fert. Dev. 19

      Pages: 230-230

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] Abnormal distribution of chromosomes in first division of nuclear transferred mouse embryos.2007

    • Author(s)
      Kawasumi M, et. al.
    • Journal Title

      J. Reprod. Dev. 53

      Pages: 615-622

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] Primate embryonic stem cells proceed to early gametogenesis in vitro.2007

    • Author(s)
      Teramura T, et. al.
    • Journal Title

      Cloning Stem Cells 9

      Pages: 144-156

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] H1foo is indispensable for meiotic maturation of the mouse oocyte.2007

    • Author(s)
      Furuya M, et. al.
    • Journal Title

      J. Reprod. Dev. 53

      Pages: 895-902

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] A mouse and embryonic stem cells derived from a single embryo.2007

    • Author(s)
      Teramura T, et. al.
    • Journal Title

      Cloning Stem Cells 9

      Pages: 485-494

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] Expression of transcription factors Cdx2 and Oct3/4 in mouse somatic nuclear transfer embryos.2007

    • Author(s)
      Nishiwaki M, et. al.
    • Journal Title

      Mem. Inst. Adv. Technol., Kinki Univ. 12

      Pages: 33-42

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] Abnormal distribution of chromosomes in first division of nuclear transferred mouse embryos2007

    • Author(s)
      Kawasumi M, et. al.
    • Journal Title

      J.Reprod.Dev 53

      Pages: 615-622

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Transgenic siRNA increases the disease duration of ALS in a mouse model2007

    • Author(s)
      Yokota T, et. al.
    • Journal Title

      Archives Neurol 64

      Pages: 145-146

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Hlfoo is indispensable for meiotic maturation of the mouse oocyte2007

    • Author(s)
      Furuya M, et. al.
    • Journal Title

      J.Reprod.Dev 53

      Pages: 895-902

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Journal Article] A mouse and embryonic stem cells derivedfrom a single embryo2007

    • Author(s)
      Teramura T, et. al.
    • Journal Title

      Cloning Stem Cells 9

      Pages: 485-494

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Transgenic siRNA increases the disease duration of ALS in amouse model.2007

    • Author(s)
      Yokota T et al.
    • Journal Title

      Archives Neurol. 64

      Pages: 145-146

    • Related Report
      2006 Annual Research Report
  • [Journal Article] Differentiation of hepatocyte-like cells from mouse embryonic stem cells in a monolayer culture system.2007

    • Author(s)
      Mitani T et al.
    • Journal Title

      Reprod. Fert. Dev. 19

      Pages: 230-230

    • Related Report
      2006 Annual Research Report
  • [Journal Article] Abnormal distribution of chromosomes in first division of nuclear transferred mouse embryos.2007

    • Author(s)
      Kawasumi M et al.
    • Journal Title

      J. Reprod. Dev. (in press)

    • Related Report
      2006 Annual Research Report
  • [Journal Article] Primate embryonic stem cells proceed to early gametogenesis in vitro.2007

    • Author(s)
      Teramura T et al.
    • Journal Title

      Cloning Stem Cells (accepted)

    • Related Report
      2006 Annual Research Report
  • [Journal Article] Inhibition of Oct4 expression in mouse preimplantation embryos using morpholino antisense oligonucleotides2006

    • Author(s)
      Tsuji I, et. al.
    • Journal Title

      Tohoku J.Exp.Med. 208

      Pages: 333-342

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
    • Peer Reviewed
  • [Journal Article] Inhibition of Oct4 expression in mouse preimplantation embryos using morpholino antisense oligonucleotides.2006

    • Author(s)
      Tsuji I, et. al.
    • Journal Title

      Tohoku J. Exp. Med. 208

      Pages: 333-342

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] Inhibition of Oct4 expression in mouse preimplantation embryos using morpholino antisense oligonucleotides.2006

    • Author(s)
      Tsuji I et al.
    • Journal Title

      Tohoku J. Exp. Med. 208

      Pages: 333-342

    • Related Report
      2006 Annual Research Report
  • [Presentation] Expression of transcription factors specific to the trophoblast lineage in mouse somatic nuclear transfer embryos2008

    • Author(s)
      Mitani T, et. al.
    • Organizer
      34th Annual Conference of the International Embryo Transfer Society
    • Place of Presentation
      Denver,U.S.A.
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] Effects of trichostatin A on DNA methylation in cloned bovine embryos2008

    • Author(s)
      Iwamoto D, et. al.
    • Organizer
      34th Annual Conference of the International Embryo Transfer Society
    • Place of Presentation
      Denver,U.S.A.
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] Production of cloned bovine embryos derived from amniotic cells of pregnant cows2008

    • Author(s)
      Taniguchi S, et. al.
    • Organizer
      34th Annual Conference of the International Embryo Transfer Society
    • Place of Presentation
      Denver,U.S.A.
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] In vitroにおける単為発生/雄性発生胚由来ES細胞からの三胚葉由来機能細胞の獲得2008

    • Author(s)
      小野寺 勇太, 他
    • Organizer
      第7回日本再生医療学会総会
    • Place of Presentation
      名古屋
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] 雌性単為発生胚と雄性発生胚由来ES細胞から誘導したインスリン産生細胞の機能性の検討2008

    • Author(s)
      武内 大輝, 他
    • Organizer
      第7回日本再生医療学会総会
    • Place of Presentation
      名古屋
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] Expression of Bcrp1 mRNA isoforms during in vitro differentiation of mouse embryonic stem cells.(accepted)2008

    • Author(s)
      Kawamura H, et. al.
    • Organizer
      第41回日本発生生物学会(国際発生生物学会共催)
    • Place of Presentation
      徳島
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] Expression of stem and germ cell markers in juvenile bovine testis and its primary culture.(accepted)2008

    • Author(s)
      Moriki K, et. al.
    • Organizer
      第41回日本発生生物学会(国際発生生物学会共催)
    • Place of Presentation
      徳島
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] Expression of the genes implicated in the embryonic development during peri-implantation period in mouse somatic cell nuclear transfer embryos.(accepted)2008

    • Author(s)
      Morita M, et. al.
    • Organizer
      第41回日本発生生物学会(国際発生生物学会共催)
    • Place of Presentation
      徳島
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] Expression of the genes implicated in the development of the trophoblast lineage in mouse somatic cell nuclear transfer embryos.(accepted)2008

    • Author(s)
      Mitani T, et. al.
    • Organizer
      16th International Congress of Animal Reproduction
    • Place of Presentation
      Budapest,Hungary
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] ABCトランスポーターBcrp1 mRNAアイソフォームの発現様式(発表予定)2008

    • Author(s)
      川村 紘子, 他
    • Organizer
      第26回日本受精着床学会総会
    • Place of Presentation
      福岡
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] 幹細胞マごカーおよび生殖細胞マーカーを用いた若齢ウシ清爽な居並びに初代培養したウシ精巣細胞におけるゴノサイトおよび精原幹細胞の同定(発表予定)2008

    • Author(s)
      森木 甲子郎, 他
    • Organizer
      第26回日本受精着床学会総会
    • Place of Presentation
      福岡
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] トリコスタチンA処理によるマウス体細胞核移植胚の着床初期における転写因子の発現(発表予定)2008

    • Author(s)
      森田 真裕, 他
    • Organizer
      第26回日本受精着床学会総会
    • Place of Presentation
      福岡
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] Expression of transcription factors specific to the trophoblast lineage in mouse somatic nuclear transfer embryos.2008

    • Author(s)
      Mitani T, et. al.
    • Organizer
      34th Annual Conference of the International Embryo Transfer Society
    • Place of Presentation
      Denver. USA
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] Effects of trichostatin A on DNA methylation in cloned bovine embryos.2008

    • Author(s)
      Iwamoto D, et. al.
    • Organizer
      34th Annual Conference of the International Embryo Transfer Society
    • Place of Presentation
      Denver, USA
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] Production of cloned bovine embryos derived from amniotic cells of pregnant cows.2008

    • Author(s)
      Taniguchi S, et. al.
    • Organizer
      34th Annual Conference of the International Embryo Transfer Society
    • Place of Presentation
      Denver, USA
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] Expression of Bcrpl mRNA isoforms during in vitro differentiation of mouse embryonic stem cells.(accepted)2008

    • Author(s)
      Kawamura H, et. al.
    • Organizer
      41st Annual Meeting for the Japanese Society of Developmental Biologists
    • Place of Presentation
      Tokushima, Japan
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] Expression of stem and germ cell markers in juvenile bovine testis and its primary culture.(accepted)2008

    • Author(s)
      Moriki K, et. al.
    • Organizer
      41st Annual Meeting for the Japanese Society of Developmental Biologists
    • Place of Presentation
      Tokushima, Japan
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] Expression of the genes implicated in the embryonic development during pefi-implantation period in mouse somatic cell nuclear transfer embryos.(accepted)2008

    • Author(s)
      Morita M, et. al.
    • Organizer
      41st Annual Meeting for the Japanese Society of Developmental Biologists
    • Place of Presentation
      Tokushima, Japan
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] Expression of the genes implicated in the development of the trophoblast lineage in mouse somatic cell nuclear transfer embrvos.(acceoted)2008

    • Author(s)
      Mitani T, et. al.
    • Organizer
      16th International Congress of Animal Reproduction
    • Place of Presentation
      Budapest, Hungary
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] Expression of the genes ilnplicated in the development of the trophoblastlineage in mouse somatic cell nuclear transfer embryos.(accepted)2008

    • Author(s)
      Mitani T, et. al.
    • Organizer
      16th International Congress of Animal Reproduction
    • Place of Presentation
      Budapest,Hungary
    • Related Report
      2007 Annual Research Report
  • [Presentation] Expression of Berp1 mRNA isoforms during in vitro differemtiation of mouseembryonic stem cells.(accepted)2008

    • Author(s)
      Kawamura H, et. al.
    • Organizer
      第41回日本発生生物学会(国際発生生物学会共催)
    • Place of Presentation
      徳島
    • Related Report
      2007 Annual Research Report
  • [Presentation] Expression of steln and germ cell markers in juvenile bovine testis and itsprimary culture.(accepted)2008

    • Author(s)
      Moriki K, et. al.
    • Organizer
      第41回日本発生生物学会(国際発生生物学会共催)
    • Place of Presentation
      徳島
    • Related Report
      2007 Annual Research Report
  • [Presentation] Expression of the genes implicated in the embryonic development duringperi-implantation period in mouse somatic cell nuclear transfer embryos.(accepted)2008

    • Author(s)
      Morita M, et. al.
    • Organizer
      第41回日本発生生物学会(国際発生生物学会共催)
    • Place of Presentation
      徳島
    • Related Report
      2007 Annual Research Report
  • [Presentation] Expression of transcription factors specific to the trophoblast Iineagein mouse somatic nuclear transfer embryos2008

    • Author(s)
      Mitani T, et. al.
    • Organizer
      34th Annual Conference of the International Embryo Transfer Society
    • Place of Presentation
      Denver,U.S.A.
    • Related Report
      2007 Annual Research Report
  • [Presentation] Effects of trichostatin A on DNA methylation in cloned bovine embryos.2008

    • Author(s)
      Iwamoto D, et. al.
    • Organizer
      34th Annual Conference of the International Embryo Transfer Society
    • Place of Presentation
      Denver,U.S.A.
    • Related Report
      2007 Annual Research Report
  • [Presentation] Production ofcloned bovine embryos derived from amniotic cells of pregnant cows2008

    • Author(s)
      Taniguchi s, et. al.
    • Organizer
      34th Annual Conference of the International Embryo Transfer Society
    • Place of Presentation
      Denver,U.S.A.
    • Related Report
      2007 Annual Research Report
  • [Presentation] Differentiation of hepatocyte-like cells from mouse embryonic stem cells in a monolayer culture system2007

    • Author(s)
      Mitani T, et. al.
    • Organizer
      33rd Annual Conference of the International Embryo Transfer Society
    • Place of Presentation
      京都
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] Effects of trichostatin A on development of bovine somatic cell nuclear transfer embryos2007

    • Author(s)
      Iwamoto D, et. al.
    • Organizer
      33rd Annual Conference of the International Embryo Transfer Society
    • Place of Presentation
      京都
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] DNA methylation profiles of upstream elements of Oct-3/4 gene in in vitro fertilization (IVF) and somatic cell nuclear-transferred(SCNT) embryos2007

    • Author(s)
      Kawasumi M, et. al.
    • Organizer
      33rd Annual Conference of the International Embryo Transfer Society
    • Place of Presentation
      京都
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] Identification and characterization of the 5'-flanking region of three mouse maternal genes(Histone Hloo,Nucleoplasmin2,and Zygote arrest1):Transcriptional activity in mouse oocytes2007

    • Author(s)
      Tsunemoto K, et. al.
    • Organizer
      33rd Annual Conference of the International Embryo Transfer Society
    • Place of Presentation
      京都
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] Effect of aging on amounts of DNA methyltransferase mRNA in mouse spermatozoa2007

    • Author(s)
      Kato H, et. al.
    • Organizer
      33rd Annual Conference of the International Embryo Transfer Society
    • Place of Presentation
      京都
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] 単為発生ES細胞からの機能細胞の獲得2007

    • Author(s)
      小野寺 勇太, 他
    • Organizer
      第6回日本再生医療学会総会
    • Place of Presentation
      横浜
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] マウス体細胞核移植胚における転写因子Cdx2およびOct3/4の発現2007

    • Author(s)
      西脇 恵, 他
    • Organizer
      第40回日本発生生物学会大会
    • Place of Presentation
      福岡
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Annual Research Report 2007 Final Research Report Summary
  • [Presentation] マウス体細胞核移植胚における栄養外胚葉系譜での遺伝子発現解析2007

    • Author(s)
      西脇 恵, 他
    • Organizer
      第25回日本受精着床学会総会
    • Place of Presentation
      仙台
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Annual Research Report 2007 Final Research Report Summary
  • [Presentation] In vitroにおける雌雄単為発生胚由来ES細胞の分化能の解析2007

    • Author(s)
      小野寺 勇太, 他
    • Organizer
      第25回日本受精着床学会総会
    • Place of Presentation
      仙台
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Annual Research Report 2007 Final Research Report Summary
  • [Presentation] 培養ウシ栄養膜細胞の効率的な単離および核移植2007

    • Author(s)
      谷口 俊仁, 他
    • Organizer
      第14回日本胚移植研究会大会
    • Place of Presentation
      鹿児島
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] 過排卵処理したマウス卵巣におけるプロテオーム解析2007

    • Author(s)
      松本 和也, 他
    • Organizer
      第14回日本胚移植研究会大会
    • Place of Presentation
      鹿児島
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] マウス体細胞核移植胚における栄養外胚葉細胞系譜における遺伝子発現2007

    • Author(s)
      西脇 恵, 他
    • Organizer
      第100回日本繁殖生物学会大会
    • Place of Presentation
      東京
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Annual Research Report 2007 Final Research Report Summary
  • [Presentation] マウス排卵卵子における網羅的タンパク質発現(プロテオーム)解析2007

    • Author(s)
      野老 美紀子, 他
    • Organizer
      第100回日本繁殖生物学会大会
    • Place of Presentation
      東京
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Annual Research Report 2007 Final Research Report Summary
  • [Presentation] Rhophilin-2遺伝子はマウス受精卵における第1分裂の細胞質分裂に関与している2007

    • Author(s)
      松岡 俊樹, 他
    • Organizer
      第100回日本繁殖生物学会大会
    • Place of Presentation
      東京
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] モルホリノアンチセンスオリゴを用いたマウス着床前胚のOct4遺伝子発現抑制2007

    • Author(s)
      辻 勲, 他
    • Organizer
      第52回日本生殖医学会総会
    • Place of Presentation
      秋田
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Annual Research Report 2007 Final Research Report Summary
  • [Presentation] siRNA transgenic mice as a tool for studying neurological diseases2007

    • Author(s)
      Sasaguri H, et. al.
    • Organizer
      34th Annual Meeting of Society for Neuroscience
    • Place of Presentation
      San Diego,U.S.A.
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] マウスES細胞における非B型DNAによるshRNA発現活性化に対する効果2007

    • Author(s)
      三谷 匡, 他
    • Organizer
      第30回日本分子生物学会大会
    • Place of Presentation
      横浜
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Annual Research Report 2007 Final Research Report Summary
  • [Presentation] マウス体細胞核移植胚における栄養外胚葉分化に関する転写因子の発現2007

    • Author(s)
      西脇 恵, 他
    • Organizer
      第30回日本分子生物学会大会
    • Place of Presentation
      横浜
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Annual Research Report 2007 Final Research Report Summary
  • [Presentation] クロマチン工学のストラテジー2007

    • Author(s)
      大山 隆, 他
    • Organizer
      第30回日本分子生物学会大会
    • Place of Presentation
      横浜
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Annual Research Report 2007 Final Research Report Summary
  • [Presentation] クロマチン工学による外来遺伝子の安定的発現2007

    • Author(s)
      棚瀬 潤一, 他
    • Organizer
      第30回日本分子生物学会大会
    • Place of Presentation
      横浜
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Annual Research Report 2007 Final Research Report Summary
  • [Presentation] RNA干渉を用いた遺伝性疾患の遺伝子治療2007

    • Author(s)
      横田 隆徳, 他
    • Organizer
      第30回日本分子生物学会大会
    • Place of Presentation
      横浜
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Annual Research Report 2007 Final Research Report Summary
  • [Presentation] マウス受精卵における分裂機構へのrhophilin-2遺伝子の関与2007

    • Author(s)
      松岡 俊樹, 他
    • Organizer
      第30回日本分子生物学会大会
    • Place of Presentation
      横浜
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Annual Research Report 2007 Final Research Report Summary
  • [Presentation] マウスMII期卵母細胞における網羅的タンパク質発現(プロテオーム)解析2007

    • Author(s)
      野老 美紀子, 他
    • Organizer
      第30回日本分子生物学会大会
    • Place of Presentation
      横浜
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Annual Research Report 2007 Final Research Report Summary
  • [Presentation] トランスジェニックマウス(Tg マウス)由来卵子及び着床前胚における母性遺伝子のプロモーター解析2007

    • Author(s)
      遠藤 法眞, 他
    • Organizer
      第30回日本分子生物学会大会
    • Place of Presentation
      横浜
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] Differentiation of hepatocyte-like cells from mouse embryonic stem cells in a monolayer culture system.2007

    • Author(s)
      Mitani T, et. al.
    • Organizer
      33rd Annual Conference of the International Embryo Transfer Society
    • Place of Presentation
      Kyoto, Japan
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] Effects of trichostatin A on development of bovine somatic cell nuclear transfer embryos.2007

    • Author(s)
      Iwamoto D, et. al.
    • Organizer
      33rd Annual Conference of the International Embryo Transfer Society
    • Place of Presentation
      Kyoto, Japan
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] DNA methylation profiles of upstream elements of Oct-3/4 gene in in vitro fertilization(IVF) and somatic cell nuclear-transferred(SCNT) embryos.2007

    • Author(s)
      Kawasumi M, et. al.
    • Organizer
      33rd Annual Conference of the International Embryo Transfer Society
    • Place of Presentation
      Kyoto, Japan
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] Identification and characterization of the 5'-flanking region of three mouse maternal genes(Histone Hloo, Nucleoplasmin2, and Zygote arrest 1) : Transcriptional activity in mouse oocytes.2007

    • Author(s)
      Tsunemoto K, et. al.
    • Organizer
      33rd Annual Conference of the International Embryo Transfer Society
    • Place of Presentation
      Kyoto, Japan
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] Effect of aging on amounts of DNA methyltransferase mRNA in mouse spermatozoa.2007

    • Author(s)
      Kato H, et. al.
    • Organizer
      33rd Annual Conference of the International Embryo Transfer Society
    • Place of Presentation
      Kyoto, Japan
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] transgenic mice as a tool for studying neurological diseases.2007

    • Author(s)
      Sasaguri H, et. al.
    • Organizer
      34th Annual Meeting of Society for siRNA Neuroscience.
    • Place of Presentation
      San Diego, USA
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] トランスジェニックマウス(Tgマウス)由来卵子及び着床前胚における母性遺伝子のプロモーター解析2007

    • Author(s)
      遠藤 法眞, 他
    • Organizer
      第30回日本分子生物学会大会
    • Place of Presentation
      横浜
    • Related Report
      2007 Annual Research Report
  • [Presentation] Rh ophilin-2遺伝子はマウス受精卵における第1分裂の細胞質分裂に関与している2007

    • Author(s)
      松岡 俊樹, 他
    • Organizer
      第100回日本繁殖生物学会大会
    • Place of Presentation
      東京
    • Related Report
      2007 Annual Research Report
  • [Presentation] Expression of maternal gene promoter driven expression vectors in mouse oocytes and fertilized eggs2006

    • Author(s)
      Tsunemoto K, et. al.
    • Organizer
      20th IUBMB Int'l.Congr.Biochem.Mol.Biol.and 11th FAOBMB Congr.
    • Place of Presentation
      京都
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] ウシ栄養膜細胞由来核移植胚作製のための体外受精胚のバイオプシー手法の検討2006

    • Author(s)
      谷口 俊仁, 他
    • Organizer
      第13回日本胚移植研究会大会
    • Place of Presentation
      広島
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] Sperm chromatin structure assay法によるウシ精子DNA断片化の解析2006

    • Author(s)
      甲田 俊夫, 他
    • Organizer
      第13回日本胚移植研究会大会
    • Place of Presentation
      広島
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] マウス体細胞核移植胚および体外受精胚におけるOct-3/4遺伝子転写調節領域のメチル化2006

    • Author(s)
      川澄 みゆり, 他
    • Organizer
      第99回日本繁殖生物学会大会
    • Place of Presentation
      名古屋
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] 母性発現遺伝子(Histone Hloo(Hloo),Nucleoplasmin2(Npm2),Zygote arrest1(Zar1))のプロモーター領域の解析2006

    • Author(s)
      常本 和伸, 他
    • Organizer
      第99回日本繁殖生物学会大会
    • Place of Presentation
      名古屋
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] マウス体細胞核移植胚における転写因子Cdx2の発現に関する免疫細胞化学的解析2006

    • Author(s)
      西脇 恵, 他
    • Organizer
      第24回日本受精着床学会総会
    • Place of Presentation
      軽井沢
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] 母性発現遺伝子(Histone Hloo(Hloo),Nucleoplasmin2(Npm2),Zygote arrest1(Zar1))のプロモーター解析2006

    • Author(s)
      常本 和伸, 他
    • Organizer
      第24回日本受精着床学会総会
    • Place of Presentation
      軽井沢
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] 単為発生由来ES細胞からの機能細胞分化誘導2006

    • Author(s)
      小野寺 勇太, 他
    • Organizer
      第51回日本生殖医学会総会
    • Place of Presentation
      大阪
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] In vitro differentiation of mouse embryonic stem cells into hepatocyte-like cells using monolayer-culture system2006

    • Author(s)
      Mitani T, et. al.
    • Organizer
      3rd Annual Conference of Asian Reproductive Biotechnology Society
    • Place of Presentation
      Hanoi,Vietnam
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] マウス体細胞核移植胚における転写因子Cdx2の発現について2006

    • Author(s)
      西脇 恵, 他
    • Organizer
      日本分子生物学会2006フォーラム
    • Place of Presentation
      名古屋
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] Expression of maternal gene promoter driven expression vectors in mouse oocytes and fertilized eggs.2006

    • Author(s)
      Tsunemoto K, et. al.
    • Organizer
      20th IUBMB Int'l. Congr. Biochem. Mol. Biol. and 11th FAOBMB Congr.
    • Place of Presentation
      Kyoto, Japan
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] In vitro differentiation of mouse embryonic stem cells into hepatocyte-like cells using monolayer-culture system.2006

    • Author(s)
      Mitani T, et. al.
    • Organizer
      3rd Annual Conference of Asian Reproductive Biotechnology Society
    • Place of Presentation
      Hanoi, Vietnam
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Remarks] 「研究成果報告書概要(和文)」より

    • URL

      http://rais.itp.kindai.ac.jp/researchdb/

    • Related Report
      2007 Final Research Report Summary
  • [Remarks]

    • URL

      http://rais.itp.kindai.ac.jp/researchdb/

    • Related Report
      2007 Annual Research Report
  • [Patent(Industrial Property Rights)] 標的内因性遺伝子の発現が抑制されたES細胞の選抜方法及び該選抜方法を利用したトランスジェニック動物の作製方法2007

    • Inventor(s)
      横田 隆徳, 笹栗 弘貴, 水澤 英洋, 三谷 匡, 安齋 政幸
    • Industrial Property Rights Holder
      近畿大学
    • Industrial Property Number
      2007-118962
    • Filing Date
      2007-04-27
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Patent(Industrial Property Rights)] 標的内因性遺伝子の発現が抑制されたES細胞の選抜方法及び該選抜方法を利用したトランスジェニック動物の作製方法2007

    • Inventor(s)
      横田 隆徳・笹栗 弘貴・水澤 英洋・三谷 匡・安齋 政幸
    • Industrial Property Rights Holder
      近畿大学
    • Industrial Property Number
      2007-118962
    • Filing Date
      2007-04-27
    • Related Report
      2007 Annual Research Report

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Published: 2006-04-01   Modified: 2016-04-21  

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