Genes regulating B cell domionant infiltration into autoimmune sialitis lesions and their intergenic interaction

• Project/Area Number: 18580286
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Basic veterinary science/Basic zootechnical science
• Research Institution: Hokkaido University
• Principal Investigator: TAKIGUCHI Mltsuyoshi (2007-2008) Hokkaido University, 大学院・獣医学研究科, 教授 (70261336); 今野 明弘 (2006) 北海道大学, 大学院獣医学研究科, 助手 (00271651)
• Co-Investigator(Kenkyū-buntansha): 滝口 満善 酪農学園大学, 獣医学部, 教授 (70261336)
• Project Period (FY): 2006 – 2008
• Project Status: Completed (Fiscal Year 2008)
• Budget Amount: ¥4,090,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥690,000); Fiscal Year 2008: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000); Fiscal Year 2007: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2006: ¥1,100,000 (Direct Cost: ¥1,100,000)
• Keywords: 遺伝 / 自己免疫 / シェーグレン症候群 / IQIマウス / 唾液線炎 / B細胞 / Ica1 / Ifi202 / 唾液腺炎 / Ical / マウスモデル / QTL

Research Abstract

シェーグレン症候群(Sjogren's syndrome ; SS)モデルIQIマウスにおけるB細胞優勢の下顎腺炎(B細胞性下顎腺炎)を遺伝学的に解析した。その結果、B細胞性下顎腺炎の量的形質遺伝子座(QTL)は少なくとも3個有り、単独で効果を発揮する主要QTL(autoimmune sialitis in IQImice, associated locus 1 ; Asq1)が第6染色体前端に、Asq1の効果を増強するエピスタシスQTLが第3染色体後方(Asq2)に、また、逆にAsq1の効果を抑制する抵抗性遺伝子座が第1染色体後方(Asq3)に位置することを明らかになった。Asq1の1LOD区間内に位置するIca1に注目し、そのcDNA塩基配列を対照C57BL/6(B6)と比較した結果、IQI-B6間で6個の一塩基多型があり、2個のアミノ酸多型が生じていた。また、ウエスタンブロット法では、IQエマウスの血清中に抗Ica1自己抗体の存在を示すバンドカミ検出された。以上の結果より、IQIマウスにおいて、Ica1に対するB細胞自己免疫が成立していることが明らかになり、原因として、1ca1の塩基多型の可能性が挙げられた。

Research Products

• [Journal Article] Quantitative contrast-enhanced edtrasonograpby of canine spleen (2009)
  • Author(s): Nakamura K, Sasaki N, Yoshikawa M, Ohta H, Hwang Shiang-Jyi, Mimura T, Yamasaki M, Takiguchi M
  • Journal Title: Vet Radiol Ultrasound 50 Pages: 104-108
• [Journal Article] Quantitative contrast-enhanced ultrasonography of canine spleen (2009)
  • Author(s): Nakamura K, Sasaki N, Yoshika wa M, Ohta H, Hwang Shiang-J yi, Mimura. T, yamasaki M, Takiguchi M.
  • Journal Title: Vet Radiol Ultrasound 50 Pages: 104-108
  • Peer Reviewed
• [Journal Article] Aldosterone-, corticosterone-and cortisol-secreating adrenocortical carcinoma in a dog : Case report (2008)
  • Author(s): Machida T, Uchida E, Matsuda K, Hirayama K, Yoshii K, Takiguchi M, Taniyama H
  • Journal Title: J. Vet Med Sci 70 Pages: 317-820
  • Peer Reviewed
• [Journal Article] Flow cytoometry to evaluate the Wel of Babesia gibsoni parasitemia in vivo and in vitro by using the fluorescent nucleic acid stain SYT016 (2008)
  • Author(s): Yamasaki M, Hwang S-J, Ohta H, Yamato O, Maede Y, Takiguchi M
  • Journal Title: Jpn. J, Vet. Res 55 Pages: 129-138
  • Peer Reviewed
• [Journal Article] Aldosterone-, corticosterone-and cortisol-secreating adrenocortical carcinoma in a dog : Case report (2008)
  • Author(s): Machida T, Uchida E, Matsuda K, Hirayama K, Yoshii K, Takiguchi M, Taniyama H.
  • Journal Title: J Vet Med Sci 70 Pages: 317-320
  • Peer Reviewed
• [Journal Article] Flow cytometry to evaluate the level of Babesia gibsoni parasitemia in vivo and in vitro by using the fluorescent nucleic acid stain SYTO 16 (2008)
  • Author(s): Yamasaki M, Hwang S-J,. Ohta H, Yamato O, Maede Y, Takiguchi. M
  • Journal Title: Jpn J Vet Res 55 Pages: 129-136
  • NAID: 120000951648
  • Peer Reviewed
• [Journal Article] Identification of a quantitative trait locus regulating B cell-dominant infiltration into autoimmune sialitis lesions of the IQI mouse model of primary Sjtigren's syndrome (2007)
  • Author(s): KorkA2, Takiguchi M, Takada K, Usami T, Azumi K, Kubota H, Inaba M, Saegusa j., Ken Y
  • Journal Title: Immunogenetics 69 Pages: 858-859
  • Peer Reviewed
• [Journal Article] Identification of a quantitative trait locus regulating B cell-dominant infiltration into autoimmune sialitis lesions of the IQI mouse model of primary Sjogren's syndrome (2007)
  • Author(s): Konno A, Takiguchi M, Takada K, Usami T, Azumi K, Kubota H, Inaba M, Saegusa J, Kon Y
  • Journal Title: Immunogenetics 59 Pages: 853-859
  • Peer Reviewed