| Project/Area Number |
18580292
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Basic veterinary science/Basic zootechnical science
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| Research Institution | University of Miyazaki |
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Principal Investigator |
YASUDA Masahiro University of Miyazaki, Faculty of Agriculture, assistant professor (10336290)
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| Project Period (FY) |
2006 – 2007
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| Project Status |
Completed (Fiscal Year 2007)
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| Budget Amount *help |
¥3,800,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥300,000)
Fiscal Year 2007: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2006: ¥2,500,000 (Direct Cost: ¥2,500,000)
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| Keywords | ruminant / primary lymphoid organ / secondary lvmphoid organ / lymphatic follicle / B-cell / immunogrobulin gene / receptor editing / 空腸パイエル板 / 回腸パイエル板 / レセプターエデイテイング / 多様性 |
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| Research Abstract |
Ruminants' gut-associated lymphoid tissues are broadly classified into ileal and jejunal Peyer's patch. We isolated single lymphatic follicles from ileal and jejunal Peyer's patches and examined thirteen cytokines mRNA expression in the individual follicles using RT-PCR. Especially, IL-2, IL-4 and IL-13 cytokine mRNA was expressed in all examined jejunal Peyer's patch follicle, but not in ileal Peyer's patch follicle. Differences of cytokine mRNA expression facilitate good understanding the differential immune reaction of primary and secondary lymphoid organs. And then, we showed that the immunoglobulin lambda light chain receptor editing could contribute to make the dominant light chain repertoire and very high in-frame ratio of Vlambda-Jlambdal. Finally, we examined morphological characteristics of the full length of calf ileal PP using gross and microscopic anatomical techniques. Most areas of ileal PP consisted of densely packed lymphatic follicles contained very few follicular T-cell and associated with only scant interfollicular areas. However, the proximal end of ileal PP consisted of widely dispersed lymphatic follicles contained many follicular T-cell and associated with large interfollicular areas. The histological architectures of the proximal end of ileal PP strongly resembled those of the secondary lymphoid tissue in calf.
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