The role of non-coding RNAin developmental toxicity by dioxin
| Project/Area Number |
18580298
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Basic veterinary science/Basic zootechnical science
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| Research Institution | Rakuno Gakuen University |
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Principal Investigator |
TERAOKA Hiroki Rakuno Gakuen University, School of Veterinary Medicine, Professor (50222146)
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| Co-Investigator(Kenkyū-buntansha) |
ENDO Daiji Rakuno Gakuen University, School of Veterinary Medicine, Professor (40168828)
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| Project Period (FY) |
2006 – 2007
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| Project Status |
Completed (Fiscal Year 2007)
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| Budget Amount *help |
¥3,830,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥330,000)
Fiscal Year 2007: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2006: ¥2,400,000 (Direct Cost: ¥2,400,000)
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| Keywords | zebrafish / non-coding RNA / dioxin / TCD / developmental toxicology / aryl hydrocarbon receptor / cytochrome P450 / 循環毒性 |
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| Research Abstract |
1) Development of novel DOP-PCR method with transcriptome as a target: We developed a novel degenerate oligonucleotide-primed PCR (DOP-PCR) with high reproducibility. We used mono-primers for DOP-PCR, as there were many sets of inverse-repeat sequence adequate for PCR reaction in transcriptome of zebrafish (Danio rerio). Primed part of primers (specific sequence part) was designed to obtain about 10 bands for each PCR reaction, using our developed computer program.
2) Differential display approach in search for transcripst increased by TCDD : We carried out differential display based on DOP-PCR described above and found CYP1C1 and 1C2 were dramatically upregulated by TCDD exposure. Gene knockdown of CYP1C2 rescued mesencephalic circulation from TCDD-induced failure, suggesting the possible rule of CYP1C2 in TCDD developmental toxicity.
3) Application of DOP-PCR for other fish species with zebrafish primers: DOP-PCR with the primers designed for zebrafish could be used for reproducible PCR products with medaka (Oryzias latips) and other 8 fish species. The same strategy could be applicable for various mammals or birds using DOP-PCR primers designed for rodents and domestic fowls.
4) Effects of dioxin on miRNA transcriptional status: Microarray media with 272 different miRNA spots was used to clarify the effect of TCDD on miRNA transcriptinal status. About 10 miRNA species showed two-fold changes or more in trasciptational level by TCDD exposure at 48 and 72 hours post fertilization, respectively. Most of these miRNA speceis overlap between two stages.
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Report
(3 results)
Research Products
(69 results)
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[Journal Article] Functional disorder of the retina in manganese-deficient Japanese quail revealed by electroretinography using a contact lens electrode with built-in light source2008
Author(s)
Endo K, Itoh N, Maehara S, Shinozaki A, Imagawa T, Uehara M, Mizuno S, Sasaki J, Hiraga T, Teraoka, H
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Journal Title
J Vet Med Sci 70
Pages: 139-144
NAID
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] Heavy metal contamination status of Japanese cranes(Grus japonensis) in east Hokkaido, Japan -Extensive mercury pollution2007
Author(s)
Teraoka H, Kumagai Y, Iwai H, Haraguchi K, Ohba T, Nakai K, Satoh H, Sakamoto M, Momose K, Masatomi H, Hiraga, T
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Journal Title
Environ Toxicol Chem 26
Pages: 307-312
Description
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[Journal Article] Enhancement of cytotoxicity against Vero E6 cells persistently infected with SARS-CoV by Mycoplasma fermentans2007
Author(s)
Mizutani T, Fukushi S, Kenri T, Sasaki Y, Ishii K, Endoh D, Zamoto A, Saijo M, Kurane I, Morikawa, S
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Journal Title
Arch Virol 152
Pages: 1019-1025
Description
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[Journal Article] Rapid determination of viral RNA sequences in mosquitoes collected in the field2007
Author(s)
Kihara Y, Satho T, Eshita Y, Sakai K, Kotaki A, Takasaki T, Rongsriyam Y, Komalamisra N, Srisawat R, Lapcharoen P, Sumroiphon S, Iwanaga S, Ushijima H, Endoh D, Miyata T, Sakata A, Kashige N, Miake F, Fukushi S, Saijo M, Kurane I, Morikawa S, Mizutani, T
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Journal Title
J Virol Methods 146
Pages: 372-374
Description
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[Journal Article] Rapid genome sequencing of RNA viruses
Author(s)
Mizutani T, Endoh D, Okamoto M, Shirato K, Shimizu H, Arita M, Fukushi S, Saijo M, Sakai K, Lim, CK, Ito M, Nerome R, Takasaki T, Ishii K, Suzuki T, Kurane I, Morikawa S, Nishimura, H
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Journal Title
Emerg Infect Dis. 13
Pages: 322-324
NAID
Description
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[Journal Article] Muscarinic receptor subtypes involved in carbachol-induced contraction of mouse uterine smooth muscle
Author(s)
Kitazawa T, Hirama R, Masunaga K, Nakamura T, Asakawa K, Cao J, Teraoka H, Unnno T, Komori S, Yamada M, Wess J, Taneike, T
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Journal Title
Naunyn-Schmiedeberg Arch Pharmacol (in press)
NAID
Description
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[Presentation] Improved differential display analysis based on degenerate oligonucleotide- primed PCR2008
Author(s)
Teraoka H, Igarashi K, Itoh S, Ogawa A, Kubota A, Abou, Elmagd M, Hiraga T, Endoh, D
Organizer
45th Annual Meeting of Society of Toxicology
Place of Presentation
Convention Center, Seattle, WA, USA
Year and Date
2008-03-20
Description
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[Presentation] Extensive and sever mercury contamination in Japanese cranes in Eastern Hokkaido, Japan : Retrospective estimates of merucury cotamination using feathers from stuffed specimens2007
Author(s)
Teraoka H, Tagami Y, Matsumoto F, Ikoma S, Shimura R, Inoue M, Momose K, Masatomi H, Hiraga, T
Organizer
10th Annual Meeting of Japan Society of Endocrine Disrupters Research
Place of Presentation
Omiya Sonic City Hall, Saitama
Year and Date
2007-12-10
Description
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[Presentation] Mercury pollution of Japanese crane in east Hokkaido, Japan2007
Author(s)
Tagami Y, Hoshi M, Kato M, Uebayashi A, Shimura R, Inoue M, Momose K, Masatomi Y, Hiraga T, Teraoka, H
Organizer
145th Annual Meeting for the Japanese Society of Veterinary Science
Place of Presentation
Rakuno Gakuen U, Ebetsu
Year and Date
2007-09-03
Description
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[Presentation] Anatomy of trachea of Tancho, Grus japonensis2007
Author(s)
Mizuta S, Shimura S, Inoue M, Uebayashi A, Momose K, Masatomi Y, Teraoka H, Hiraga, T
Organizer
145th Annual Meeting for the Japanese Society of Veterinary Science
Place of Presentation
Rakuno Gakuen U, Ebetsu
Year and Date
2007-09-02
Description
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[Presentation] Involvement of cycloxygenase-2-thromboxane pathway in pericardial edema caused by 2, 3, 7, 8-tetrachlorodibenzo-p-dioxin in developing zebrafish2007
Author(s)
Teraoka H, Furuya M, Dong, W, Harada Y, Peterson, RE, Hiraga, T
Organizer
11th international Congress of Toxicology
Place of Presentation
Convention Center, Montreal, Canada
Year and Date
2007-07-18
Description
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[Presentation] Specific activation of aryl hydrocarbon receptor(AHR) soforms by 2, 3, 7, 8-tetrachlorodibenzo-p-dioxin in red seabream(Pagrus major) embryo2006
Author(s)
Kim, E-Y, Yamauchi M, Iwata H, Teraoka H, Hiraga T, Tanabe, S
Organizer
20th IUBMB International Congress of Biochemistry and Molecular Biology
Place of Presentation
Convention Center, Kyoto
Year and Date
2006-06-22
Description
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[Presentation] Involvement of cyclooxygenase 2 in mesencephalic circulation failure and apoptosis evoked by 2, 3, 7, 8-tetrachlorodibenzo-p-dioxin in developingzebrafish2006
Author(s)
Teraoka H, Dong, W, Kounosu N, Furuya M, Nonoyama K, Hiraga, T
Organizer
7th International Conference on Zebrafish Development and Genetics
Place of Presentation
U. Wisconsin, Madison, WI, US
Year and Date
2006-06-17
Description
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