Analysis of West Nile virus neuroinvasion using in vitro blood-brain barrier models
| Project/Area Number |
18580302
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Applied veterinary science
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| Research Institution | Hokkaido University |
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Principal Investigator |
KIMURA Takashi Hokkaido University, Research Center for Zoonosis Control, Associate Professor (90261338)
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| Co-Investigator(Kenkyū-buntansha) |
YOSHII Kentaro Hokkaido University, Graduate School of Veterinary Medicine, Assistant Professor (50421988)
SAWA Hirofumi Hokkaido University, Research Center for Zoonosis Control, Professor (30292006)
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| Project Period (FY) |
2006 – 2007
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| Project Status |
Completed (Fiscal Year 2007)
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| Budget Amount *help |
¥4,010,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥510,000)
Fiscal Year 2007: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
Fiscal Year 2006: ¥1,800,000 (Direct Cost: ¥1,800,000)
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| Keywords | virus / central nervous sysytem |
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| Research Abstract |
1. The pathway used by West Nile virus (WNV) to leave the bloodstream and invade the central nervous system is poorly understood. To investigate how WNV cross the blood-brain barrier (BBB), we used confluent human umbilical vein endothelial cells (HUVEC) cultures on transwell inserts as an in vitro BBB model.
2. The structural protein genes (C-PrM-E region) of WNV 6-LP strain and Eg 101 strain were cloned into the pCMV expression vector. Sequential transfection of WNV sub-genomic replicon having an EGFP expression cassette and the vector that expressed the structural proteins led to the secretion of virus-like particles (VLPs). VLPs established a single-round of infection without production of progeny, and the physical structure of the VLPs was similar to that of infectious virions.
3. In vitro BBB model was infected with VLPs having structural proteins of high vilulent 6LP strain (6LP-VLPs) or VLPs having structural proteins of low vilurent Eg 101 strain (Eg-VLPs). By 24 h after infection, 10% of 6LP-VLPs had crossed in vitro BBB, whereas no Eg-VLPs seemd to traverse. Thus, the ability of WNV to cross BBB may correlate at least in part with viral virulence. Exposure of HUVEC to 6LP strain did not alter the localization of tight junction protein ZO-1, indicating that the passage of WNV across the BBB is not caused by the disruption of tight junction. On the other hand, infection of HUVEC with 6LP strain was inhibited by Chlorpromazine, an inhibitor of clathrin-coated pit formation at the plasma membrane. These results suggest the possibility that WNV may cross BBB by transcytosis via a clathrin-mediated endocytic pathway.
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Report
(3 results)
Research Products
(19 results)
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[Journal Article] Pharmacological cdk inhibitor R-Roscovitine suppresses JC virus proliferation.2008
Author(s)
Orba, Y., Sunden, Y., Suzuki, T., Nagashima, K., Kimura, T., Tanaka, S., Sawa, H.
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Journal Title
Virology 370(1)
Pages: 173-83
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] Differential susceptibility of equine and mouse brain microvascular endothelial cells to equine herpesvirus 1 infection.2006
Author(s)
Hasebe, R., Kimura, T., Nakamura, K., Ochiai, K. Okazaki, K., Wada, R. and Umemura, T.
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Journal Title
Archives of Virology 151(4)
Pages: 775-86
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] Cerebellar Hypoplasia Associated with an Avian Leukosis Virus Inducing Fowl Glioma.2006
Author(s)
Toyoda, T., Ochiai, K., Hatai, M., Murakami, E., Ono, T., Kimura, T. and Umemura, T.
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Journal Title
Veterinary Pathology 43(3)
Pages: 294-301
Description
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[Journal Article] Efficacy of Intracerebral Immunization against Pseudorabies Virus in Mice.2006
Author(s)
Shin, J., Sakoda, Y., Kim, J.-H., Tanaka, T., Kida, H., Kimura, T., Ochiai, K. and Umemura, T.
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Journal Title
Microbiology and Immunology 50(10)
Pages: 823-830
NAID
Description
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[Presentation] Infectious entry of equine herpesvirus-1 into host cells through different endocytic pathways.2007
Author(s)
Hasebe, R., Kimura, T., Sawa, H., Wada, R. and Umemura, T.
Organizer
American Society for Virology 26^<th> Annual Meeting
Place of Presentation
Oregon State University, Corvallis, Oregon, U.S.A.
Year and Date
2007-07-15
Description
「研究成果報告書概要(欧文)」より
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