Investigation of the dynamics of group A rotaviruses in nature and analysis of a risk for new prevalence of these viruses
| Project/Area Number |
18580307
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Applied veterinary science
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| Research Institution | Gifu University |
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Principal Investigator |
SUGIYAMA Makoto Gifu University, Faculty of Applied Biological Sciences, Professor (80196774)
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| Co-Investigator(Kenkyū-buntansha) |
KITAGAWA Hitoshi Gifu University, Faculty ofApplied Biological Sciences, Professor (70144003)
ITO Naoto Gifu University, Faculty of Applied Biological Sdences, Associate professor (20334922)
ASANO Makoto Gifu University, Faculty of Applied Biological Sciences, Associate professor (30377692)
MIYAZAWA Kiyoshi Gifu University, Faculty of Applied Biological Sdences, Professor (20012031)
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| Project Period (FY) |
2006 – 2007
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| Project Status |
Completed (Fiscal Year 2007)
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| Budget Amount *help |
¥4,010,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥510,000)
Fiscal Year 2007: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
Fiscal Year 2006: ¥1,800,000 (Direct Cost: ¥1,800,000)
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| Keywords | Group A rotavirus / Epidemiologieal investigati / Cattle / Wild boar / P genotvne / Reassortment / Infectious dynamics |
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| Research Abstract |
We investigated the prevalence and infection of group A rotaviruses in the healthy calves and wild boars which were thought that various rotaviruses are contagious at high frequency by our previous seroepidemiological study. One of 135 samples of intestinal tract contents of wild boars, which were captured in Aichi, Gifu and Gunma Prefecture from 2003 to 2006, was positive in RT-nested PCR method for VP4 gene of rotavirus. From the analysis of the partial nucleotide sequence of its VP4 gene, it is genetically close to GBV strain bearing P[3] which was isolated from a goat in Korea. We also investigated rotavirus VP4 genes in 2146 fecal samples collected from healthy calves in Gifu Prefecture in June, 2007 from April, 2006. Of these, 44 positive samples for rotavirus VP4 gene were analyzed genetically and these viruses were thought to belong to P[5], P [11], P[14] and new P genotypes. The rotavirus genes were detectable in adult cattle, which have a low sensitivity for rotaviruses, and in the summer of the non-season. From the result in analysis of partial VP7 gene of these viruses, it was found that a reassortment event occurred in rotaviruses detected among healthy calves in the same farm. In conclusion, we observed that rotavirus infection and shedding occurred in healthy adult cattle in the summer season. Moreover, we are able to show the possibility of outbreak of diarrhea by rotaviruses in asymptomatic animals and by new type viruses produced by a reassortment.
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Report
(3 results)
Research Products
(5 results)
