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Analysis of cDNA library of Echionococcus multilocularis adult worm for its control measures in dog

Research Project

Project/Area Number 18580313
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Applied veterinary science
Research InstitutionHokkaido Institute of Public Health

Principal Investigator

YAGI Kinpei  Hokkaido Institute of Public Health, 感染症センター生物科学部, 主任研究員 (70414323)

Co-Investigator(Kenkyū-buntansha) KATOH Yoshinobu  北海道立衛生研究所, 感染症センター生物科学部, 主任研究員 (00414326)
MATSUMOTO Jun  日本大学, 生物資源科学部, 講師 (70296169)
OKU Yuzaburo  国立大学法人北海道大学・, (連合)獣医学研究科, 准教授 (60133716)
SUZUKI Tomohiro  北海道立衛生研究所, 感染症センター生物科学部, 研究職員 (10414327)
GOTO Akiko  北海道立衛生研究所, 感染症センター生物科学部, 研究職員 (60414322)
Research Collaborator KOUGUCHI Hirokazu  北海道立衛生研究所, 感染症センター生物科学部, 研究職員 (50435567)
Project Period (FY) 2006 – 2008
Project Status Completed (Fiscal Year 2008)
Budget Amount *help
¥4,030,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥630,000)
Fiscal Year 2008: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2007: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2006: ¥1,300,000 (Direct Cost: ¥1,300,000)
Keywords人獣共通感染症 / エキノコックス症 / イヌ / 成虫 / cDNAライブラリー / 血清診断 / ワクチン
Research Abstract

北海道のエキノコックス症(多包虫症)は、住民の健康への脅威となっている。本症の実験的研究は、本症の解決に必須であるが、危険を伴うため情報が限られていた。我々は、感染リスクのある成虫ステージに着目し、特殊感染実験施設を用いて、新鮮なエキノコックス成虫のcDNAライブラリーを作成し、対策に有用な遺伝子と蛋白の探索を行った。その結果、1035個のクローンの塩基配列を決定し、その中から新規遺伝子emY162を発見、その有用性を実験的に検証した。

Report

(4 results)
  • 2008 Annual Research Report   Final Research Report ( PDF )
  • 2007 Annual Research Report
  • 2006 Annual Research Report
  • Research Products

    (17 results)

All 2009 2008 2007

All Journal Article (6 results) (of which Peer Reviewed: 6 results) Presentation (9 results) Patent(Industrial Property Rights) (2 results)

  • [Journal Article] Characterization of emY162 encoding an immunogenic protein cloned from an adult worm-specific cDNA library of Echino- coccus multilocularis2008

    • Author(s)
      KATOH Y, KOUGUCHI H, MASTUMOTO J, GOTO A, SUZUKI T, OKU Y, YAGI K,
    • Journal Title

      Biochim Biophys Acta 1780(1)

      Pages: 1-6

    • Related Report
      2008 Final Research Report
    • Peer Reviewed
  • [Journal Article] Experimental studies on Echinococcus multilocularis in Japan, focusing on biohazardous stages of the parasite.2008

    • Author(s)
      MATSUMOTO J, YAGI K.
    • Journal Title

      Exp Parasitol 119(4)

      Pages: 534-41

    • Related Report
      2008 Final Research Report
    • Peer Reviewed
  • [Journal Article] Experimental studies on Echinococcus multilocularis in Japan, focusing on biohazardous stages of the parasite2008

    • Author(s)
      MATSUMOTO J, YAGI K
    • Journal Title

      Experimental Parasitology 119

      Pages: 534-41

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Characterization of emY162 encoding an immunogenic protein cloned from an adultworm-specific cDNA library of Echinococcus multilocularis2008

    • Author(s)
      Katoh, et. al.
    • Journal Title

      Biochim Biophys Acta. 1780

      Pages: 1-6

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Journal Article] The vaccination potential of EMY162 antigen against Echinococcus multilocularis infection2007

    • Author(s)
      KOUGUCHI H, MASTUMOTO J, KATOH Y, OKU Y, SUZUKI T. and YAGI K.
    • Journal Title

      Biochem Biophys Res Commun. 363(4)

      Pages: 915-20

    • Related Report
      2008 Final Research Report
    • Peer Reviewed
  • [Journal Article] The vaccination potential of EMY162 antigen against Echinococcus multi locularis infection2007

    • Author(s)
      Kouguchi, et. al.
    • Journal Title

      Biochem Biophys Res Commun. 363

      Pages: 915-920

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Presentation] 多包条虫metacestodeに対するワクチン開発に関する研究2009

    • Author(s)
      党 志勝、八木欣平、奥祐三郎、孝口裕一、梶野喜一、渡辺淳一、中尾亮、Hiroyaki Wakagari, Atsushi Toyoda、杉本千尋
    • Organizer
      日本獣医学会学術集会
    • Place of Presentation
      栃木県宇都宮市 栃木県総合文化センター
    • Year and Date
      2009-04-03
    • Related Report
      2008 Final Research Report
  • [Presentation] EMY162 protein as a vaccine candidate to reduce level of alveolar hydaitid disease.2008

    • Author(s)
      KATOH Y, KOUGUCHI H, MASTUMOTO J, SUZUKI T and YAGI K.
    • Organizer
      II World Conference of Magic Bullets(EHRLICH II)
    • Place of Presentation
      Nuremberg, Germany
    • Year and Date
      2008-10-09
    • Related Report
      2008 Final Research Report
  • [Presentation] 組換えEMY162抗原の中間宿主における多包条虫感染防御効果2008

    • Author(s)
      八木 欣平
    • Organizer
      第145回日本獣医学会学術集会
    • Place of Presentation
      神奈川県相模原市
    • Year and Date
      2008-03-29
    • Related Report
      2007 Annual Research Report
  • [Presentation] 組換えEMY162抗原の中間宿主における多包条虫感染防御効果2008

    • Author(s)
      孝口裕一,松本 淳,加藤芳伸,奥 祐三郎,鈴木智宏,八木欣平
    • Organizer
      第145回日本獣医学会
    • Place of Presentation
      つくば市 麻布大学
    • Related Report
      2008 Final Research Report
  • [Presentation] EMY162 protain as a vaccine candidate to reduce level of alveolar hydaitid disease2008

    • Author(s)
      KATOH Y, et al.
    • Organizer
      II World Conference of Magic Bullets(EHRLICH II)
    • Place of Presentation
      Neuremberg, Germany
    • Related Report
      2008 Annual Research Report
  • [Presentation] 組換えEMY162抗原の中間宿主における多包条虫感染防御効果2008

    • Author(s)
      孝口裕一, 松本淳, 他
    • Organizer
      第145回日本獣医学会
    • Place of Presentation
      神奈川県相模原市麻布大学キャンパス
    • Related Report
      2008 Annual Research Report
  • [Presentation] Characterization of a novel secretory-protein cloned from an adult worm-specific cDNA library of Echinococcus multilocularis2007

    • Author(s)
      Katoh, et. al.
    • Organizer
      22nd International Congress of Hydatidology
    • Place of Presentation
      ギリシア共和国アテネ
    • Year and Date
      2007-05-18
    • Related Report
      2007 Annual Research Report
  • [Presentation] Characterization of novel secretory- protain cloned from an adult worm-specific cDNA library of Echinococcus multilo- cularis2007

    • Author(s)
      KATOH Y, KOUGUCHI H, MASTUMOTO J, GOTO A, SUZUKI T, OKU Y and YAGI K.
    • Organizer
      XXII International congress of hydatidology
    • Place of Presentation
      Athens, Greek
    • Year and Date
      2007-05-16
    • Related Report
      2008 Final Research Report
  • [Presentation] 多包虫症対策に有用な新規タンパク質探索のための多包条虫成虫cDNAライブラリー構築2007

    • Author(s)
      加藤芳伸, 孝口裕一, 松本 淳, 後藤明子, 鈴木智宏, 奥 祐三郎,八木欣平
    • Organizer
      第76回日本寄生虫学会
    • Place of Presentation
      吹田市 大阪大学医学部
    • Related Report
      2008 Final Research Report
  • [Patent(Industrial Property Rights)] 多包条虫由来の新規蛋白質2007

    • Inventor(s)
      奥祐三郎、松本淳、八木欣平、加藤芳伸、孝口裕一、鈴木智宏、後藤明子
    • Industrial Property Rights Holder
      国立大学法人 北海道大学、北海道
    • Industrial Property Number
      2007-022365
    • Filing Date
      2007-01-31
    • Related Report
      2008 Final Research Report
  • [Patent(Industrial Property Rights)] 多包条虫由来の新規蛋白質2007

    • Inventor(s)
      八木欣平 他6名
    • Industrial Property Rights Holder
      北海道, 北海道大学
    • Industrial Property Number
      2007-022365
    • Filing Date
      2007-01-31
    • Related Report
      2006 Annual Research Report

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Published: 2006-04-01   Modified: 2016-04-21  

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