| Budget Amount *help |
¥2,970,000 (Direct Cost: ¥2,700,000、Indirect Cost: ¥270,000)
Fiscal Year 2007: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2006: ¥1,800,000 (Direct Cost: ¥1,800,000)
|
|---|
| Research Abstract |
Adiponectin is a representative adipokine produced by adipocytes. Plasma adiponectin concentration decresses in obese people, and it is closely related to pathophysiology of diabetes mellitus in human medicine. We carried out cDNA cloning of feline adiponectin and adiponectin receptor-1 (AD-R1) using RT-PCR method, and revealed that amino-acid sequences of these molecules were highly identical to those of other species. Adiponectin expressed exclusively in adipose tissue, and AD-R1 expressed in all organs tested. Especially, extra-cellular domains of AD-R1 was completely (100%) identical to that of human, suggesting that the cat can be a experimental model for the human diabetes study focusing on AD-R1 as a molecular target.
Adiponectin ELISA kit for mice and rats using polyclonal antibody was tested for measurement of feline adiponectin. We demonstrated reproducibility, dilution linearity and recovery test, then it was concluded that this kit was available for measurement of feline adiponectin. Using this ELISA kit, we measured the plasma samples collected from patients visiting the veterinary medical center. We compared adiponectin levels among the cats with various body condition scores, and it was revealed that blood adiponectin is decreased in obese cats in a same way with human beings.
We measured 2 diabetic cats visiting the veterinary medical center repeatedly, and found that plasma adiponectin values increase when plasma fructosamine decrease, and decrease when fructosamine increase suggesting the possibility that adiponectin can be another index for monitoring of diabetes in diabetic cats.
|
