| Project/Area Number |
18580338
|
|---|
| Research Category |
Grant-in-Aid for Scientific Research (C)
|
| Allocation Type | Single-year Grants |
|---|
| Section | 一般 |
|---|
| Research Field |
Applied molecular and cellular biology
|
|---|
| Research Institution | Nara Institute of Science and Technology |
|---|
Principal Investigator |
KATO Ko Nara Institute of Science and Technology, Graduate School of Biological Sciences, Assistant Professor (80283935)
|
|---|
| Project Period (FY) |
2006 – 2007
|
| Project Status |
Completed (Fiscal Year 2007)
|
| Budget Amount *help |
¥3,950,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2007: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2006: ¥2,000,000 (Direct Cost: ¥2,000,000)
|
|---|
| Keywords | Translational regulation / Abiotic stress / Arabidopsis thaliana / DNA microarray / Polvsome profile |
|---|
| Research Abstract |
Drastic translational changes have been observed in plants under various abiotic stress conditions such as elevated temperature, high salinity, drought, and O2 deprivation. Common features of responses at the translational level to above stresses are global repression of translation and the existence of a fraction of differentially regulated mRNAs that are refractory to the repression. Less is known, however, about the regulation or regulatory networks at the translational levels compared to the transcriptional levels.
In this study, we carried out genome-wide analysis of translational regulation in Arabidopsis (Arabidopsis thaliana) suspension cell culture subjected to elevated temperature (heat stress) and high salinity (salt stress) conditions using DNA microarray by comparing the translation state of individual mRNAs before and after the application of a stimulus. On that basis, translational regulations under the both stress conditions were compared. It was expected that this comparative analysis would allow gaining a better understanding of the mechanism, the regulatory network, or biological significance of translational control in plants response to abiotic stress than previous reports focused on a single abiotic stress. Our results showed that both elevated temperature and high salinity elicit the cells to translation repression of most mRNA species, while some were defied to or sensitive to the repression. Notably, we found that the behavior of individual mRNA species at the translational levels were similar between in plants response to both stresses.
|
