Detection and identification of cancer-related glycoproteins by glycomic approaches

• Project/Area Number: 18590045
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Physical pharmacy
• Research Institution: National Institute of Health Sciences
• Principal Investigator: KAWASAKI Nana National Institute of Health Sciences, 生物薬品部, 室長 (20186167)
• Co-Investigator(Kenkyū-buntansha): TAKAKURA Disuke 国立医薬品食品衛生研究所, 生物薬品部, 博士研究員
• Project Period (FY): 2006 – 2008
• Project Status: Completed (Fiscal Year 2008)
• Budget Amount: ¥4,100,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥600,000); Fiscal Year 2008: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2007: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2006: ¥1,500,000 (Direct Cost: ¥1,500,000)
• Keywords: 腫瘍マーカー / 大腸癌 / 乳癌 / 膵癌 / ヘテロ核リボ核酸タンパク質 / 糖鎖 / 糖鎖抗原 / 診断マーカー / グライコミクス / LC / MS / シアリルルイスX / 癌細胞 / 電気泳動 / レクチンカラム

Research Abstract

大腸癌等に特異的に発現しているシアリルルイスx付加タンパク質として,ヘテロ核リボ核酸タンパク質A3が示唆された.

Research Products

• [Journal Article] The significance of glycosylation analysis in development of biopharmaceuticals (2009)
  • Author(s): Nana Kawasaki, Satsuki Itoh, Noritaka Hashii, Daisuke Takakura, Yuan Qin, Huang Xiaoyu, Teruhide Yamaguchi
  • Journal Title: Biol. Pharm. Bull. (印刷中)
  • NAID: 130000117140
  • Peer Reviewed
• [Journal Article] Glycosylation analysis of IgLON family proteins in rat brain by liquid chromatography/multiple-stage mass spectrometry (2008)
  • Author(s): Satsuki Itoh, Akiko Hachisuka, Nana Kawasaki, Noritaka Hashii, Reiko Teshima, Takao Hayakawa, Toru Kawanishi, Teruhide Yamaguchi
  • Journal Title: Biochemistry 47 Pages: 10132-10154
  • Peer Reviewed
• [Journal Article] Harazono, Daisuke Takakura, Teruhide Yamaguchi Mass spectrometry for analysis of carbohydrate heterogeneity in characterization and evaluation of glycoprotein products (2008)
  • Author(s): Nana Kawasaki, Satsuki Itoh, Noritaka Hashii, Akira
  • Journal Title: Trends in Glycosci. Glycotech. 20 Pages: 97-116
  • Peer Reviewed
• [Journal Article] Laminin-1 is a novel carrier glycoprotein of non-sulfated HNK-1 epitope in mouse kidney, (2008)
  • Author(s): Yasuhiko Kizuka, Kyoko Kobayashi, Shinako Kakuda, Nakajima Yukari, Nana Kawasaki, Shougo Oka
  • Journal Title: Glycobiology (In press)
  • Peer Reviewed
• [Journal Article] Glycosylation and ligand-binding activities of rat plasma fibronectin during liver regeneration after partial hepatectomy. (2008)
  • Author(s): Kotone Sano, Miho Asahi, Maiko Yanagiboshi, Noritaka Hashii, Satsukiltoh, Nana Kawasaki, Yasunori Miyamoto, Haruko Ogawa
  • Journal Title: Carbohydrate Res. (In press)
  • Peer Reviewed
• [Journal Article] Study on the quality control of cell therapy product:Determination of N-glycolylneuraminic acid incorporated into human cells by nano-flow liquid chromatography/Furrier transformation ion cyclotron resonance mass spectrometry. (2007)
  • Author(s): Noritaka Hashii, Nana Kawasaki, Yukari Matsuishi, Masashi Toyoda, Yoko Katagiri, Satsuki Itoh, Akira Harazono, Akihiro Umezawa, Teruhide Yamaguchi
  • Journal Title: J.Chromatogr.A 1160 Pages: 263-269
  • Peer Reviewed
• [Journal Article] Glycosylation-dependent interaction of Jacalin with CD45 induces T lymphocyte activiation and Th1/Th2 cytokine secretion (2007)
  • Author(s): Baba, M., Ma, B.Y., Nonaka, M., Matsuishi, Y., Hirano, M., Nakamura, N., Kawasaki, N., Kawasaki, N., Kawasaki, T.
  • Journal Title: J. Leukoc. Biol. (In press)
• [Journal Article] Mass spectrometry of glycoprotein glycans : HUPO HGPI (Human Proteome Organization Human Disease Glycomics/Proteome Initiative) Multi-institutional study (2007)
  • Author(s): Wada, Y., Azadi, P., Costello, C.E., Dell, A., Geyer, R., Kakehi, K., Karlsson, N.G., Kato, K., Kawasaki, N., et al.
  • Journal Title: Glycobiology 17 Pages: 411-422
• [Journal Article] LC/MS^n for glycoproteome analysis : Glycosylation analysis and peptide sequencing of glycopeptides (2007)
  • Author(s): Kawasaki, N., Itoh, S., Yamaguchi, T.
  • Journal Title: Methods in Molecular Biology (In press)
• [Journal Article] Deletion of core fucosylation on α3β1 integrin down-regulates its functions (2006)
  • Author(s): Zhao, Y., Itoh, S., Wang, X., Isaji, T., Miyoshi, E., Kariya, Y., Miyazaki, K., Kawasaki, N., Taniguchi, N., Gu, J.
  • Journal Title: J. Biol. Chem. 281 Pages: 38343-38350
• [Journal Article] N-acetylglucosaminyltransferase III antagonizes the effect of N-acetylglucosaminyltransferase V on α3β1 integrin-mediated cell migration. (2006)
  • Author(s): Zhao, Y., Nakagawa, Y., Itoh, S., Inamori, K., Isaji, T., Kariya, Y., Kondo, A., Miyoshi, E., Miyazaki, K., Kawasaki, N., Taniguchi, N., Gu, J.
  • Journal Title: J. Biol. Chem. 281 Pages: 32122-32130
• [Journal Article] LC/MS strategies in the characterization of glycoproteins, (2006)
  • Author(s): Kawasaki, N., Itoh, S., Kawanishi, T.
  • Journal Title: Encyclopedia of mass spectrometry 8 Pages: 923-930
• [Presentation] LC/MSを用いた糖鎖の微量かつ網羅的解析と創薬への応用 (2008)
  • Author(s): 川崎 ナナ
  • Organizer: 日本薬学会第128年会
  • Place of Presentation: 横浜
• [Book] 「抗体医薬品の最前線」植田充美監修,シーエムシー(東京) (2007)
  • Author(s): 川崎 ナナ, 伊藤 さつき, 山口 照英
  • Publisher: 抗体のLC/MS.2007
• [Book] バイオ医薬品の品質, 安全性評価. 早川堯夫監修
  • Author(s): 川崎ナナ, 早川堯夫
  • Publisher: エル・アイ・シー(印刷中)
• [Patent(Industrial Property Rights)] 腫瘍の検査法 (2009)
  • Inventor(s): 川崎ナナ, 高倉大輔, 橋井則貴, 山口照英
  • Industrial Property Rights Holder: 財団法人ヒューマンサイエンス振興財団
  • Filing Date: 2009-05-28