Analysis of lipid-droplet-formation mechanisms related withlipid-metabolic enzymes in lipid droplets

• Project/Area Number: 18590072
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Biological pharmacy
• Research Institution: Iwate Medical University (2007-2008); Teikyo University (2006)
• Principal Investigator: FUJIMOTO Yasuyuki Iwate Medical University, 薬学部, 准教授 (60317724)
• Project Period (FY): 2006 – 2008
• Project Status: Completed (Fiscal Year 2008)
• Budget Amount: ¥4,160,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥660,000); Fiscal Year 2008: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2007: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2006: ¥1,300,000 (Direct Cost: ¥1,300,000)
• Keywords: 脂肪滴 / アシルCoA合成酵素 / 中性脂質 / 脂肪酸 / 脂質代謝 / GFP / 蛍光標識 / 中世脂質 / リパーゼ

Research Abstract

本研究によって、哺乳類細胞の脂肪滴上で中性脂質合成が行われている可能性が提示され、その際脂肪滴上のアシルCoA合成酵素が重要な役割を果たすことが示唆された。ヒト肝由来培養細胞をモデルとした場合、細胞の周縁部に存在する脂肪滴で中性脂質合成が盛んであるとの状況証拠が得られた。また、脂肪滴特異的脂質代謝酵素に緑色蛍光蛋白を融合させた蛋白は、生細胞中で発現させると脂肪滴表面に蛍光が観察されることから、脂肪滴の動態観察に有効と考えられた。

Research Products

• [Journal Article] Molecular pathology in atherosclerosis : the mechanism how cholesteryl ester accumulates in atheromatous aorta (2008)
  • Author(s): Takano T., Itabe H., Mori M., Kimura J., Nakagami K., Sato R., Hashita R., Yagyu Y., Mineo C., Amanuma K., Imanaka T., Higashi Y., Fujimoto Y. and Fujita E
  • Journal Title: Yakugaku Zasshi 128 Pages: 1383-1401
  • NAID: 110006935748
• [Journal Article] Functional dissection of transmembrane domains of human TAP-like(ABCB9) (2008)
  • Author(s): Kamakura A, Fujimoto Y., Motohashi Y., Ohashi K., Ohashi-Kobayashi A. and Maeda M
  • Journal Title: Biochem. Biophys. Res. Commun 377(3) Pages: 847-851
  • Peer Reviewed
• [Journal Article] Up-regulation of microtubule-associated protein 2 accompanying the filial imprinting of domestic chicks (Gallus gallus domesticus) (2008)
  • Author(s): Yamaguchi S., Fujii-Taira I., Murakami A., Hirose N., Aoki N., Izawa E., Fujimoto Y., Takano T., Matsushima T. and Homma K. J
  • Journal Title: Brain Res. Bulletin 76(3) Pages: 282-288
  • Peer Reviewed
• [Journal Article] J. Gene expression profile in cerebrum in the filial imprinting of domestic chicks(Gallus gallus domesticus) (2008)
  • Author(s): Yamaguchi S., Fujii-Taira I., Katagiri S., Izawa E., Fujimoto Y., Takeuchi H., Takano T., Matsushima T. and Homma K.
  • Journal Title: Brain Res. Bulletin 76(3) Pages: 275-281
  • Peer Reviewed
• [Journal Article] アシル-CoA合成酵素に着目した細胞内中性脂質蓄積機構の研究 (2008)
  • Author(s): 藤本康之
  • Journal Title: 医科学応用研究財団研究報告 25 Pages: 308-311
• [Journal Article] Gene expression profile in cerebrum in the filial imprinting of domestic chicks (Gallus gallus domesticus). (2008)
  • Author(s): Yamaguchi S., Fujii-Taira I., Kalagiri S., Izawa E., Fujimoto Y., Takeuchi H., Takano T., Malsushima T., Homma K, J.
  • Journal Title: Brain Res. Bulletin 76(3) Pages: 275-281
  • Peer Reviewed
• [Journal Article] Up-regulation of microtubule-associated protein 2 accompanying the filial imprinting of domestic chicks (Gallus gallus domesticus). (2008)
  • Author(s): Yamaguchi S., Fujii-Taira I., Murakami A., Hirose N., Aoki N., Izawa E., Fujimoto Y. Takano T., Matsushima T., Homma K. J.
  • Journal Title: Brain Res. Bulletin 76(3) Pages: 282-288
  • Peer Reviewed
• [Journal Article] Functional dissection of transmembrane domains of human TAP-like (ABCB9) (2008)
  • Author(s): Kamakura A., Fujimoto Y., Motohashi Y., Ohashi K., Ohashi-Kobayashi A, Maeda M.
  • Journal Title: Biochem. Biophys. Res. Commun. 377(3) Pages: 847-851
  • Peer Reviewed
• [Journal Article] Molecular pathology in atherosclerosis : the mechanism how cholesterylester accumulates in atheromatous aorta. (2008)
  • Author(s): Takano T., Itabe H., Mori M., Kimura J., Nakagami K., Sato R., Hashita R., Yagyu Y., Mineo C., Amanuma K., Imanaka T., Higashi Y., Fuiimoto Y., Fujita E.
  • Journal Title: Yakugaku Zasshi. 128 Pages: 1383-1401
• [Journal Article] Involvement of ACSL in local synthesis of neutral lipids in cytoplasmic lipid droplets in human hepatocyte HuH7 (2007)
  • Author(s): Fujimoto Y., Itabe H., Kinoshita T., Homma K. J., Onoduka J., Mori M., Yamaguchi S., Makita M., Higashi Y., Yamashita A. and Takano T.
  • Journal Title: J. Lipid Res 48(6) Pages: 1280-1292
  • Peer Reviewed
• [Journal Article] J. In vivo gene transfer into newly-hatched chick brain by electroporation (2007)
  • Author(s): Yamaguchi S., Katagiri S., Hirose N., Fujimoto Y., Mori M., Fujii-Taira I., Takano T., Matsushima T. and Homma K.
  • Journal Title: NeuroReport 18(8) Pages: 735-739
  • Peer Reviewed
• [Journal Article] Involvement of ACSL in local synthesis of neutral lipids in cytoplasmic lipid droplets in human hepatocyte HuH7. (2007)
  • Author(s): Fujimoto Y., Itabe H., Kinoshita T., Homma K.J., Onoduka J., Mori M., Yamaguchi S., Makita M., Higashi Y., Yamashita A. and Takano T.
  • Journal Title: J. Lipid Res. 48 Pages: 1280-1292
  • Peer Reviewed
• [Journal Article] In vivo gene transfer into newly-hatched chick brain by electroporation. (2007)
  • Author(s): Yamaguchi S., Katagiri S., Hirose N., Fujimoto Y., Mori M., Fujii-Taira I., Takano T., Matsushima T., Homma K.J.
  • Journal Title: Neuro Report (in press)
• [Journal Article] Long-chain Fatty Acids Induce Lipid Droplet Formation in a Cultured Human Hepatocyte in a Manner Dependent of Acyl-CoA Synthetase (2006)
  • Author(s): Fujimoto Y., Onoduka J., Homma K. J., Yamaguchi S., Mori M., Higashi Y., Makita M., Kinoshita T., Noda J., Itabe H., and Takano T.
  • Journal Title: Biol. Pharm. Bull 29(11) Pages: 2174-2180
  • NAID: 110004840387
  • Peer Reviewed
• [Journal Article] Crystal structure of Vigna radiata cytokinin-specific binding potein in complex with zeatin (2006)
  • Author(s): Pasternak O., Bujacz G. D., Fujimoto Y., Hashimoto Y., Jelen F., Otlewski J., Sikorski M.M., and Jaskolski M.
  • Journal Title: Plant Cell 18 Pages: 1-13
  • Peer Reviewed
• [Journal Article] Crystal structure of Vigna radiata cytokinin-specific binding potein in complex with zeatin. (2006)
  • Author(s): Pasternak O., Bujacz G.D., Fujimoto Y., Hashimoto Y., Jelen F., Otlewski J., Sikorski M., Jaskolski M.
  • Journal Title: Plant Cell 18 Pages: 1-13
• [Journal Article] Long-chain Fatty Acids Induce Lipid Droplet Formation in a Cultured Human Hepatocyte in a Manner Dependent of Acyl-CoA Synthetase. (2006)
  • Author(s): Fujimoto Y., Onoduka J., Homma K.J., Yamaguchi S., Mori M., Higashi Y., Makita M., Kinoshita T., Noda J., Itabe H., Takano T.
  • Journal Title: Biol.Pharm.Bull 29 Pages: 2174-2180
  • NAID: 110004840387
• [Presentation] ABC輸送体TAPL(ABCB9)のlysosome局在 (2008)
  • Author(s): 藤本康之、前田正知
  • Organizer: 第31回日本分子生物学会年会・第81回日本生化学会大会合同大会
  • Place of Presentation: 神戸
  • Year and Date: 2008-12-12
• [Presentation] ABC輸送体TAPL(ABCB9)のlysosome局在 (2008)
  • Author(s): 藤本康之, 前田正知
  • Organizer: 第31回日本分子生物学会年会・第81回日本生化学会大会 合同大会
  • Place of Presentation: 神戸国際会議場
  • Year and Date: 2008-12-12
• [Presentation] 心筋細胞分化系におけるgata-4遺伝子の発現制御 (2008)
  • Author(s): 石橋拓也、大橋一晶、牛島弘雅、藤本康之、前田正知
  • Organizer: 第47回日本薬学会東北支部大会
  • Place of Presentation: 岩手医科大学薬学部
  • Year and Date: 2008-10-26
• [Presentation] 脂肪滴のアシルCoA合成酵素 (2007)
  • Author(s): 本康之、板部洋之、木下哲昭、本間光一、山口真二、小野塚潤、山下純、前田正知、高野達哉
  • Organizer: 第30回日本分子生物学会年会・第80回日本生化学会大会合同大会
  • Place of Presentation: パシフィコ横浜
  • Year and Date: 2007-12-15
• [Presentation] MAP2(Microtubule-Associated Protein 2)の遺伝子発現と鳥類記憶学習の相関 (2007)
  • Author(s): 松井隆剛、村上明男、山口真二、藤本康之、松島俊也、高野達哉、本間光一
  • Organizer: 日本薬学会第127年会
  • Place of Presentation: 富山国際会議場
  • Year and Date: 2007-03-28
• [Presentation] In vivoエレクトロポレーション法を用いたニワトリ雛大脳領域への遺伝子導入 (2007)
  • Author(s): 片桐幸子、廣瀬直樹、山口真二、藤本康之、松島俊也、高野達哉、本間光一
  • Organizer: 日本薬学会第127年会
  • Place of Presentation: 富山国際会議場
  • Year and Date: 2007-03-28
• [Presentation] PROTEASOME-DEPENDENT PATHWAY IS INVOLVED IN RAPID DEGRADATION OF ADIPOSE DIFFERENTIATION-RELATED PROTEIN DURING REGRESSION OF INTRACELLULAR LIPID DROPLETS (2006)
  • Author(s): Sasabe N., Takano T., Fujimoto Y., Itabe H., and Arai H.
  • Organizer: 20th IUBMB International Congress of Biochemistry and molecular Biology
  • Place of Presentation: Kyoto
• [Presentation] ADIPOSE DIFFERENTIATION-RELATED PROTEIN(ADRP)IS A KEY COMPONENT OF LIPID STORAGE AND MOBILIZATION IN MACROPHAGE FOAM CELLS (2006)
  • Author(s): Itabe H., Masuda Y, Mori M., Fujimoto Y., Arai H. and Takano T.
  • Organizer: 20th IUBMB International Congress of Biochemistry and molecular Biology
  • Place of Presentation: Kyoto