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Analysis of gene expression profile in Werner and questionable Werner syndromes

Research Project

Project/Area Number 18590080
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Biological pharmacy
Research InstitutionKanazawa Medical University

Principal Investigator

ISHIGAKI Yasuhito  Kanazawa Medical University, 付置研究所, 講師 (20232275)

Co-Investigator(Kenkyū-buntansha) TAKEGAMI Tsutomu  金沢医科大学, 付置研究所, 教授 (10113490)
二階堂 修  金沢学院短期大学, 食物栄養学科, 教授 (60019669)
松井 忍  金沢医科大学, 付置研究所, 教授 (00064600)
Co-Investigator(Renkei-kenkyūsha) NIKAIDO Osamu  金沢学院短期大学, 食物栄養学科, 教授 (60019669)
MATSUI Shinobu  金沢医科大学, 付置研究所, 教授 (00064600)
Project Period (FY) 2006 – 2008
Project Status Completed (Fiscal Year 2008)
Budget Amount *help
¥4,010,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥510,000)
Fiscal Year 2008: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2007: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2006: ¥1,800,000 (Direct Cost: ¥1,800,000)
Keywords早期老化 / Werner症候群 / DNAマイクロアレイ / RNAi / 変異遺伝子 / 老化 / マイクロアレイ / 遺伝子 / NMD
Research Abstract

ウェルナー症候群(WS)は日本人に高頻度でみられる劣性遺伝疾患で早期老化症状を特徴とする。本研究ではWSの網羅的な遺伝子発現解析を行うと同時に、臨床症状を示しながら細胞の分裂寿命が短縮していない亜群の早期老化症状を引き起こす原因遺伝子あるいは発現クラスターの解析を行うことによって、新しい老化の分子モデルを提案することである。培養細胞における網羅的遺伝子発現データを解析し老化プロファイルを明らかにした。

Report

(4 results)
  • 2008 Annual Research Report   Final Research Report ( PDF )
  • 2007 Annual Research Report
  • 2006 Annual Research Report
  • Research Products

    (42 results)

All 2009 2008 2007 2006 Other

All Journal Article (19 results) (of which Peer Reviewed: 16 results) Presentation (19 results) Book (2 results) Remarks (2 results)

  • [Journal Article] Ventromedial hypothalamic lesions change the expression of neuron-related genes and immune-related genes in rat liver.2009

    • Author(s)
      Kiba T, Kintaka Y, Suzuki Y, Nakata E, Ishigaki Y, Inoue S
    • Journal Title

      Neurosci Lett 455

      Pages: 14-16

    • Related Report
      2008 Final Research Report
    • Peer Reviewed
  • [Journal Article] スタテンの免疫調節作用2009

    • Author(s)
      高橋孝
    • Journal Title

      Medical Practice 26

      Pages: 681-681

    • Related Report
      2008 Annual Research Report
  • [Journal Article] The Kampo Medicines Orengedokuto, Bofutsushosan and Boiogito have different activities to regulate gene expressions in differentiated rat white adipocytes: Comprehensive analysis of genetic profiles.2008

    • Author(s)
      Yamakawa J, Ishigaki Y, Takanao F, Takahashi T, Yoshida J, Moriya J, Takata T, Tatsuno T, Sasaki K, Ohta T, Takegami T, Yoshizaki F
    • Journal Title

      Biol Pharm Bull 31

      Pages: 2083-2089

    • Related Report
      2008 Final Research Report
    • Peer Reviewed
  • [Journal Article] Multiple shRNA expressions in a single plasmid vector improve RNAi against the XPA gene.2008

    • Author(s)
      Nagao A, Zhao X, Takegami T, Nakagawa H, Matsui S, Matsunaga T, Ishigaki Y
    • Journal Title

      Biochem Biophys Res Commun 370

      Pages: 301-305

    • Related Report
      2008 Final Research Report
    • Peer Reviewed
  • [Journal Article] Changes of neuron-specific and apoptosis gene expression levels after ventromedial hypothalamic lesions in rat intestine.2008

    • Author(s)
      Kiba T, Kintaka Y, Suzuki Y, Nakata E, Osaka T, Ishigaki Y, Inoue S
    • Journal Title

      Exp Biol Med 233

      Pages: 1368-1378

    • Related Report
      2008 Final Research Report
    • Peer Reviewed
  • [Journal Article] The liquid culture filtrates of entomogenous fungus Paecilomyces tenuipes and its glycoprotein constituent protects against anemia in mice treated with 5-fluorouracil.2008

    • Author(s)
      Takata T, Tanaka T, Yahagi N, Yahagi R, Tsuchida H, Ishigaki Y, Tomosugi N, Fushiya S, Takano F, Ohta T
    • Journal Title

      Biol Pharm Bull 31

      Pages: 1565-1573

    • Related Report
      2008 Final Research Report
    • Peer Reviewed
  • [Journal Article] SNPs ジェノタイピングアレイを用いた超微細染色体構造異常検出にむけた基礎的検討 -先天性多発奇形・精神発達遅延の症例の場合-2008

    • Author(s)
      尾崎 守、石垣靖人
    • Journal Title

      金沢医科大誌 33

      Pages: 1-12

    • Related Report
      2008 Final Research Report
    • Peer Reviewed
  • [Journal Article] Changes of neuron-specific and apoptosis gene expression levels after ventromedial hypothalamic lesions in rat intestine2008

    • Author(s)
      Takayoshi Kiba
    • Journal Title

      Exp. Biol. Med. 233

      Pages: 1368-1378

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Journal Article] 膵癌細胞における塩酸ゲムシタビンによるアポトーシス関連遺伝子発現の変化.2007

    • Author(s)
      島崎 猛夫, 石垣 靖人, 源 利成, 元雄 良治
    • Journal Title

      膵臓 22

      Pages: 14-20

    • NAID

      10024366179

    • Related Report
      2008 Final Research Report
    • Peer Reviewed
  • [Journal Article] High-quality RNA extraction from rat pancreas for microarray analysis.2007

    • Author(s)
      Kiba T, Kintaka Y, Nakada E, Suzuki Y, Inoue S, Ishigaki Y
    • Journal Title

      Pancreas 35

      Pages: 98-100

    • Related Report
      2008 Final Research Report
    • Peer Reviewed
  • [Journal Article] 膵癌細胞における塩酸ゲムシタビンによるアポトーシス関連遺伝子発現の変化2007

    • Author(s)
      島崎 猛夫ら
    • Journal Title

      膵臓 22-1

      Pages: 14-20

    • Related Report
      2006 Annual Research Report
  • [Journal Article] High quality RNA extraction from rat pancreas for microarray analysis.2007

    • Author(s)
      Kiba et al.
    • Journal Title

      Pancreas (In press)

    • Related Report
      2006 Annual Research Report
  • [Journal Article] Gene expression profiling in rat liver after VMH lesioning.

    • Author(s)
      Kiba T, Kintaka Y, Suzuki Y, Nakata E, Ishigaki Y, Inoue S
    • Journal Title

      Exp Biol Med

    • Related Report
      2008 Final Research Report
    • Peer Reviewed
  • [Journal Article] Isolation of N-acetylneuraminic acid and N-glycolylneuraminic acid from Pleurocybella porrigens.

    • Author(s)
      Takata Takanobu
    • Journal Title

      J. Health Sci. (印刷中)

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Ventromedial hypothalamic lesions change the expression of neuron-related genes and immune-related genes in rat liver.

    • Author(s)
      Takayoshi Kiba
    • Journal Title

      Neurosci. Lett. (印刷中)

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Gene expression profiling in rat liver after VMH lesioning.

    • Author(s)
      Takayoshi Kiba
    • Journal Title

      Exp. Biol. Med. (印刷中)

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Multiple shRNA expressions in a single plasmid vector improve RNAi against the XPA gene

    • Author(s)
      Akihiro Nagao
    • Journal Title

      Biochemical and Biophysical Research Communications (印刷中)

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Bioreductive activation of quinone anti-tumor drugs by mitochondrial VDAC1

    • Author(s)
      Eriko Shimamura
    • Journal Title

      Anatomical Science International (印刷中)

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Journal Article] SNPsジェノタイピングアレイを用いた超微細染色体構造異常検出にむけた基礎的検討

    • Author(s)
      尾崎 守
    • Journal Title

      金沢医科大学雑誌 (印刷中)

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Presentation] 早期老化症候群の網羅的遺伝子発現解析2009

    • Author(s)
      石垣靖人、中川秀昭、竹上 勉、友杉直久、後藤眞
    • Organizer
      日本薬学会第129年会
    • Place of Presentation
      京都
    • Year and Date
      2009-03-27
    • Related Report
      2008 Annual Research Report 2008 Final Research Report
  • [Presentation] Werner症候群におけるマイクロRNAの網羅的解析2008

    • Author(s)
      石垣靖人
    • Organizer
      第29回北陸臨床遺伝研究会
    • Place of Presentation
      永平寺町
    • Year and Date
      2008-11-15
    • Related Report
      2008 Final Research Report
  • [Presentation] SNPsアレイによるCNV解析の基礎的検討2008

    • Author(s)
      尾崎 守, 石垣靖人, 柿沼宏明
    • Organizer
      北陸臨床遺伝研究会
    • Place of Presentation
      永平寺町
    • Year and Date
      2008-11-15
    • Related Report
      2008 Final Research Report
  • [Presentation] 膵癌に対するGSK3β阻害剤とジェムシタビンによる治療効果:臨床試験を目指した基礎的検討2008

    • Author(s)
      島崎猛夫, 石垣靖人, 中谷直喜, 中島日出夫, 友杉直久, 田中卓二, 麦 威, 川上和之, 源 利成, 元雄良治
    • Organizer
      第67回日本癌学会学術総会
    • Place of Presentation
      名古屋
    • Year and Date
      2008-10-29
    • Related Report
      2008 Final Research Report
  • [Presentation] 日本脳炎ウイルス感染による宿主遺伝子発現およびmiRNA動態への影響2008

    • Author(s)
      竹上 勉, 村上 学, 佐藤杏子, 太田隆英, 石垣靖人
    • Organizer
      第15回トガ・フラビ・ペスチウイルス研究会
    • Place of Presentation
      岡山
    • Year and Date
      2008-10-25
    • Related Report
      2008 Final Research Report
  • [Presentation] SNPs検出アレイによるCNV解析の基礎的検討2008

    • Author(s)
      尾崎 守, 石垣靖人, 柿沼宏明
    • Organizer
      日本人類遺伝学会
    • Place of Presentation
      横浜
    • Year and Date
      2008-09-28
    • Related Report
      2008 Final Research Report
  • [Presentation] 膵癌の臨床試験を目指したGSK3β阻害剤と酸塩ゲムシタビンの併用療法に関する基礎的解析2008

    • Author(s)
      島崎猛夫, 石垣靖人, 夏 啓勝, 中谷直喜, 中島日出夫, 友杉直久, 田中卓二, 麦 威, 川上和之, 源 俊成, 元雄良治
    • Organizer
      第19回日本消化器癌発生学会総会
    • Place of Presentation
      別府
    • Year and Date
      2008-08-28
    • Related Report
      2008 Final Research Report
  • [Presentation] Analysis of gene expression in Japanese encephalitis virus infected cells using microarray.2008

    • Author(s)
      T.Takegami, M.Murakami, M.Sato, T.Ota, Y.Ishigaki
    • Organizer
      15th International Congress of Virology
    • Place of Presentation
      トルコ共和国イスタンブール
    • Year and Date
      2008-08-15
    • Related Report
      2008 Final Research Report
  • [Presentation] ヒト細胞におけるNMD関連因子の免疫電顕法による解析2008

    • Author(s)
      石垣靖人, 趙霞, 栗原孝行, 和泉伸一
    • Organizer
      第10回RNAミーティング
    • Place of Presentation
      札幌
    • Year and Date
      2008-07-24
    • Related Report
      2008 Final Research Report
  • [Presentation] 日本脳炎ウイルス感染に伴う宿主遺伝子発現の網羅的解析2008

    • Author(s)
      竹上 勉, 村上 学, 佐藤 杏子, 太田隆英, 石垣靖人
    • Organizer
      第12回神経ウイルス研究会
    • Place of Presentation
      屋久島
    • Year and Date
      2008-07-18
    • Related Report
      2008 Final Research Report
  • [Presentation] SNPs検出型DNAマイクロアレイによる染色体解析2007

    • Author(s)
      石垣靖人, 尾崎守, 柿沼宏明
    • Organizer
      第28回北陸先天異常研究会
    • Place of Presentation
      金沢
    • Year and Date
      2007-12-01
    • Related Report
      2008 Final Research Report
  • [Presentation] Combined effect of gemcitabine and GSK3βinhibitor against human pancreas cancer: GSK3βas a new therapeutic molecular target.2007

    • Author(s)
      T.Shimasaki, Y.Ishigaki, X.Zhao X, N.Tomosugi, T.Tanaka, W.Mai, K.Kawakami, T.Minamoto, Y.Motoo
    • Organizer
      38th Annual Meeting of the American Pancreatic Association
    • Place of Presentation
      米国シカゴ
    • Year and Date
      2007-11-01
    • Related Report
      2008 Final Research Report
  • [Presentation] Combined effect of gemcitabine and GSK3βinhibitor against pancreas cancer resulting in altered gene expression profiles.2007

    • Author(s)
      T.Shimasaki, Y.Ishigaki, X.Zhao, N.Tomosugi, T.Tanaka, W.Mai, K.Kawakami, T.Minamoto, Y.Motoo
    • Organizer
      The Second Meeting of Asian-Oceanic Pancreatic Association(AOPA)
    • Place of Presentation
      神戸
    • Year and Date
      2007-10-18
    • Related Report
      2008 Final Research Report
  • [Presentation] Combined effect of gemcitabine and GSK3βinhibitor against human pancreas cancer: GSK3β as a new therapeutic target.2007

    • Author(s)
      T.Shimasaki, Y.Ishigaki, H.Nakajima, N.Tomosugi, T.Tanaka, K.Kawakami, T.Minamoto, Y.Motoo
    • Organizer
      第66回日本癌学会学術総会
    • Place of Presentation
      横浜
    • Year and Date
      2007-10-04
    • Related Report
      2008 Final Research Report
  • [Presentation] 塩酸ゲムシダビンとGSK3β阻害剤併用による膵癌細胞の増殖抑制効果とその分子機構2007

    • Author(s)
      島崎猛夫, 中島日出夫, 石垣靖人, 本西 哲, 友杉直久, 田中卓二, 川上和之, 源 利成, 元雄良治
    • Organizer
      第38回日本膵臓学会大会
    • Place of Presentation
      福岡
    • Year and Date
      2007-06-28
    • Related Report
      2008 Final Research Report
  • [Presentation] DNA microarray analysis of Werner syndrome and questionable Werner syndrome.2007

    • Author(s)
      Yasuhito Ishigaki
    • Organizer
      The 1^<st> Anglo-Japanese Werner syndrome consortium meeting.
    • Place of Presentation
      東京
    • Year and Date
      2007-03-20
    • Related Report
      2008 Final Research Report
  • [Presentation] DNAマイクロアレイによるナンセンス変異遺伝子検出2006

    • Author(s)
      石垣靖人, 趙 霞, 柿沼宏明, 竹上 勉, 松井 忍
    • Organizer
      第27回北陸先天異常研究会学術集会
    • Place of Presentation
      内灘
    • Year and Date
      2006-11-26
    • Related Report
      2008 Final Research Report
  • [Presentation] 遺伝疾患原因遺伝子の探索2006

    • Author(s)
      石垣靖人
    • Organizer
      金沢医科大学医学会第42回学術集会
    • Place of Presentation
      内灘
    • Year and Date
      2006-07-15
    • Related Report
      2008 Final Research Report
  • [Presentation] DNA microarray analysis with NMD suppression in humana cells carrying nonsense mutation.2006

    • Author(s)
      Y.Ishigaki, Z.Xia
    • Organizer
      20th International Congress of Biochemistry and Molecular Biology
    • Place of Presentation
      京都
    • Year and Date
      2006-06-19
    • Related Report
      2008 Final Research Report
  • [Book] Optimized gene silencing by co-expression of multiple shRNAs in a single vector. "RNA Interference: From Biology to Clinical Application" in Methods in Molecular Biology Series

    • Author(s)
      Ishigaki Y, Nagao A, Matsunaga T
    • Publisher
      Humana press
    • Related Report
      2008 Final Research Report
  • [Book] RNA Interference : From Biology to Clinical Application

    • Author(s)
      Yasuhito Ishigaki
    • Publisher
      Humana press(印刷中)
    • Related Report
      2008 Annual Research Report
  • [Remarks] 石垣靖人: 網羅的解析手法による疾患解析、Ingenuity Pathways Analysis (IPA) ユーザーミーティング、東京(2008年8月28日、招待講演)

    • Related Report
      2008 Final Research Report
  • [Remarks] 石垣靖人:チップテクノロジーによる疾患解析、ポストゲノム研究フォーラム、金沢(2007年6月28日、招待講演)

    • Related Report
      2008 Final Research Report

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Published: 2006-04-01   Modified: 2016-04-21  

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