| Budget Amount *help |
¥4,090,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥690,000)
Fiscal Year 2009: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000)
Fiscal Year 2008: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2007: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2006: ¥1,100,000 (Direct Cost: ¥1,100,000)
|
|---|
| Research Abstract |
Lipid droplets are cellular lipid storage organelles involved not only in lipid homeostasis but also in a variety of diseases. However, little is known about cellular factors involved in the biogenesis of lipid droplets. In this study, we performed proteomic profiling of lipid droplet proteins in human liver cell lines and identified more than 80 proteins by 1D-SDSPAGE/MALDI-TOF mass spectrometry (MS) or direct nano-flow liquid chromatography-MS/MS. I also found that acetyl-CoA carboxylase 1 has an essential role in lipid droplet biogenesis in cultured mammalian cells.
|
