A study on lipid droplet biogenesis in mammalian cells
| Project/Area Number |
18590092
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Biological pharmacy
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| Research Institution | National Institute of Infectious Diseases |
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Principal Investigator |
FUKASAWA Masayoshi National Institute of Infectious Diseases, 細胞化学部, 室長 (20291130)
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| Project Period (FY) |
2006 – 2009
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| Project Status |
Completed (Fiscal Year 2009)
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| Budget Amount *help |
¥4,090,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥690,000)
Fiscal Year 2009: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000)
Fiscal Year 2008: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2007: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2006: ¥1,100,000 (Direct Cost: ¥1,100,000)
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| Keywords | 生化学 / 脂肪滴 / 脂質 / アセチルCoAカルボキシラーゼ / 変異株 |
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| Research Abstract |
Lipid droplets are cellular lipid storage organelles involved not only in lipid homeostasis but also in a variety of diseases. However, little is known about cellular factors involved in the biogenesis of lipid droplets. In this study, we performed proteomic profiling of lipid droplet proteins in human liver cell lines and identified more than 80 proteins by 1D-SDSPAGE/MALDI-TOF mass spectrometry (MS) or direct nano-flow liquid chromatography-MS/MS. I also found that acetyl-CoA carboxylase 1 has an essential role in lipid droplet biogenesis in cultured mammalian cells.
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Report
(6 results)
Research Products
(22 results)
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[Journal Article] Proteomic profiling of lipid droplet proteins in hepatoma cell lines expressing hepatitis C virus core protein.2006
Author(s)
Sato, S., Fukasawa, M., Yamakawa, Y., Natsume, T., Suzuki, T., Shoji, I., Aizaki, H., Miyamura, T., Nishijima, M.
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Journal Title
J. Biochem. 139
Pages: 921-930
NAID
Related Report
Peer Reviewed
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[Journal Article] Proteomic profiling of lipid droplet proteins in hepatoma cell lines expressing hepatitis C virus core protein2006
Author(s)
Sato, S., Fukasawa, M., Yamakawa, Y., Natsume, T., Suzuki, T., Shoji, I., Aizaki, H., Miyamura, T. and shijima, M
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Journal Title
J. Biochem 139
Pages: 921-930
NAID
Related Report
Peer Reviewed
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