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Development of screening system for antibody-displayed phages by photo panning method : isolation of anti-amyloid antibody

Research Project

Project/Area Number 18590114
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Drug development chemistry
Research InstitutionKyushu University of Health and Welfare

Principal Investigator

SADAKANE Yutaka  Kyushu University of Health and Welfare, School of Pharmaceutical Sciences, Full-time instructor (60293304)

Co-Investigator(Kenkyū-buntansha) KAWAHARA Masahiro  Kyusyu University of Health Welfare, School of Pharmaceutical Sciences, Professor (40224828)
KONOHA Keiko  Kyusyu University of Health Welfare, School of Pharmaceutical Sciences, Research associate (00369175)
Project Period (FY) 2006 – 2007
Project Status Completed (Fiscal Year 2007)
Budget Amount *help
¥3,910,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥510,000)
Fiscal Year 2007: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
Fiscal Year 2006: ¥1,700,000 (Direct Cost: ¥1,700,000)
Keywordsphotoreaction / diazirine / antibody-displayed phage / amyloid / proteome / panning / screening / peptide polymerization
Research Abstract

We developed a method for an efficient cloning of display phages using photoreactive ligand, which was based on the combination of two heterogeneous techniques, photoaffinity labeling and panning of display phages. Photoaffinity labeling introduces a cross-link between a ligand and its specific binding proteins for fixing their affinity relationship through a covalent bond. We used 3-ary1-3-1rifluoromethyl-diazirine as a photophore because it appears to come closest to satisfy the criteria required for useful photophore. In our photochemical panning, the photoreactive ligand baring carbene-generating photophore, diazirine functions as a barb on a hook, onto which ligands are placed as fish bait Thus, the cross-linked phages stay on the ligand even after intensive washing, whereas almost all the unspecific binding phages are removed.
In this study, we developed the photoreactive panning method for screening of antibody-displayed phage, which was categorized in M13 filamentous phage. We succeeded the panning of antibody-displayed phage by using avidine-magnet beads and photoreactive antigen peptide, which had diazirine as a photophore, and biotin at N-terminal for a binding tag for avidine. Our developed panning methods enabled to clone the target displayed phage with higher sensitivity than the traditional panning did.
We found that a certain peptide fragment of prion protein inhibited the growth of rat primary culture cell of hippocannpus, and also that N-terminal structure of Alzheimer's beta-amyloid peptide altered spontaneously. We synthesized the peptide N-temiinal of which altered and tried to isolate the antibody-displayed phage that was reacted with such alternation. The synthesized peptide was immunized to five mice and their serums were obtained. We confirmed the production of antibody in the senims by ELISA with antigen.

Report

(3 results)
  • 2007 Annual Research Report   Final Research Report Summary
  • 2006 Annual Research Report
  • Research Products

    (110 results)

All 2008 2007 2006 2005 Other

All Journal Article (46 results) (of which Peer Reviewed: 20 results) Presentation (59 results) Remarks (2 results) Patent(Industrial Property Rights) (3 results) (of which Overseas: 2 results)

  • [Journal Article] Endeavors to make the photophore,diazirine easy to use2007

    • Author(s)
      Y.sadakane
    • Journal Title

      YAKUGAKU ZASSHI 127

      Pages: 1693-1699

    • NAID

      110006402953

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
    • Peer Reviewed
  • [Journal Article] ヒトタンパク質アミノ酸配列中に潜在する生物活性ペプチド2007

    • Author(s)
      中込 和哉, 定金 豊
    • Journal Title

      ぶんせき 2007-2

      Pages: 66-73

    • NAID

      10018521643

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
    • Peer Reviewed
  • [Journal Article] Differential susceptibility of alpha A-and alpha B-crystallin to gamma-ray irradiation2007

    • Author(s)
      N.Fujii, T.Nakamura, Y.Sadakane, T.Saito, N.Fujii
    • Journal Title

      BBA-Proteins and Proteomics 1774

      Pages: 345-350

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
    • Peer Reviewed
  • [Journal Article] Age related changes of alpha-crystallin aggregate in human lens2007

    • Author(s)
      N.Fujii, Y.Shimmyo, M.Sakai, Y.Sadakane, T.Nakamura, Y.Morimoto, T.Kinouchi, Y.Goto, K.Lampi.
    • Journal Title

      Amino acids 32

      Pages: 87-94

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
    • Peer Reviewed
  • [Journal Article] Aluminum and human health:its intake,bioavailability and neurotoxicity2007

    • Author(s)
      M.Kawahara, K.Konoha, T.Nagata, Y.Sadakane
    • Journal Title

      Biomed.Res.Trace Elements 18

      Pages: 111-120

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
    • Peer Reviewed
  • [Journal Article] Protective activity of the extracts from Japanese eel(Anguilla japonica)against zinc-induced neuronal cell death:Carnosine and an unknown substance2007

    • Author(s)
      Y.Sadakane, K.Konoha, T.Nagata, M.Kawahara
    • Journal Title

      Trace Nutrient Res. 24

      Pages: 1-4

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
    • Peer Reviewed
  • [Journal Article] Neurotoxicity of aluminum hydroxyl polymer on primary cultured neurons of rat cerebral cortex2007

    • Author(s)
      K.Konoha, T.Nagata, Y.Sadakane, M.Kawahara
    • Journal Title

      Biomed.Res.Trace Elements 18

      Pages: 393-397

    • NAID

      10021097781

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
    • Peer Reviewed
  • [Journal Article] Protective substances against zinc-induced neuronal death after ischemia:carnosine a target for drug of vascular type of dementia2007

    • Author(s)
      M.Kawahara, K.Konoha, T.Nagata, Y.Sadakane
    • Journal Title

      Recent Patents on CNS Drug Discovery 2

      Pages: 145-149

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
    • Peer Reviewed
  • [Journal Article] Neurotoxicity of zinc:the involvement of calcium homeostasis and carnosine2007

    • Author(s)
      M.Kawahara, K.Konoha, Y.Sadakane
    • Journal Title

      Biomed Res Trace Elements 18

      Pages: 26-34

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
    • Peer Reviewed
  • [Journal Article] Endeavors to make the photophore, diazirine easy to use.2007

    • Author(s)
      Y. Sadakane
    • Journal Title

      YAKUGAKU ZASSHJ(with peer review.) 127

      Pages: 1693-1699

    • NAID

      110006402953

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] Bioactive peptides derived from amino acids sequences in human proteins2007

    • Author(s)
      K. Nakagomi, Y. Sadakane
    • Journal Title

      Bunseki, (with peer review) 2

      Pages: 66-73

    • NAID

      10018521643

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] Differential susceptibility of alpha A-and alpha B-crystallin to gammaray irradiation.2007

    • Author(s)
      N. Fujii, T. Nakamura, Y. Sadakane, T. Saito, N. Fujii
    • Journal Title

      BBA-Proteins and Proteomics 1774

      Pages: 345-350

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] Age related changes of aipha-crystallin aggregate in human lens.2007

    • Author(s)
      N. Fujii, Y. Shimmyo, M. Sakai, Y. Sadakane, T. Nakamura, Y. Morimoto, T. Kinouchi, Y. Goto, K. Lampi
    • Journal Title

      Amino acids, (with peer review.) 32

      Pages: 87-94

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] Aluminum and human health : its intake, bioavailability and neurotoxicity.2007

    • Author(s)
      M. Kawahara, K. Konoha, T. Nagata, Y. Sadakane
    • Journal Title

      Biomed. Res. Trace Elements, (with peer review.) 18

      Pages: 111-120

    • NAID

      130004456835

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] Protective activity of the extracts from Japanese eel(Anguilla japonica) against zinc-induced neuronal cell death : Carnosine and an unknown substance.2007

    • Author(s)
      Y. Sadakane, K. Konoha, T. Nagata, M. Kawahara
    • Journal Title

      Trace Nutrient Res.(with peer review.) 24

      Pages: 1-4

    • NAID

      40015808263

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] Neurotoxicity of aluminum hydroxyl polymer on primary cultured neurons of rat cerebral cortex.2007

    • Author(s)
      K. Konoha, T. Nagata, Y. Sadakane, M. Kawahara
    • Journal Title

      Biomed. Res. Trace Elements, (with peer review.) 18

      Pages: 393-397

    • NAID

      10021097781

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] Protective substances against zinc-induced neuronal death after ischemia : camosine a target for drug of vascular type of dementia.2007

    • Author(s)
      M. Kawahara, K. Konoha, T. Nagata, Y. Sadakane
    • Journal Title

      Recent Patents on CNS Drug Discovery, (with peer review.) 2

      Pages: 145-149

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] Neurotoxicity of zinc : the involvement of calcium homeostasis and camosine.2007

    • Author(s)
      M. Kawahara, K. Konoha, Y. Sadakane
    • Journal Title

      Biomed. Res. Trace Elements, (with peer review.) 18

      Pages: 26-34

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] Protective activity of the extracts from Japanese eel(Anguilla japonica)against zinc-induced neuronal cell death:Carnosine and an unknown substance2007

    • Author(s)
      Sadakane Y., Konoha K., Nagata T., Kawahara M
    • Journal Title

      Trace Nutrient Res. 24

      Pages: 111-120

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Neurotoxicity of aluminum hydroxyl polymer on primary cultured neurons of rat cerebral cortex2007

    • Author(s)
      Konoha K, Nagata T, Sadakane Y, Kawahara M
    • Journal Title

      Biomed.Res.Trace Elements 18

      Pages: 393-397

    • NAID

      10021097781

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Endeavors to make the photophore,diazirine easy to use2007

    • Author(s)
      Y.Sadakane
    • Journal Title

      YAKUGAKU ZASSHI 127

      Pages: 1693-1699

    • NAID

      110006402953

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Simple and Versatile Method for Tagging Phenyldiazirine Photophores2006

    • Author(s)
      H.Nakashima, M.Hashimoto, Y.Sadakane, T.Tomohiro, Y.Hatanaka
    • Journal Title

      J.Am.Chem.Soc. 128

      Pages: 15092-15093

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary 2006 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Photoaffinity labeling of opioid-binding cell-adhesion molecule (OBCAM) by phenyldiazirine-integrated ligand2006

    • Author(s)
      Y.sadakane, E.Kitamoto, T.Tomohiro, K.Konoha, M.Kawahara, Y.Hatanaka
    • Journal Title

      J.KUHW 8

      Pages: 179-185

    • NAID

      110006407510

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] Phenyldiazirine unit for converting peptides,proteins,DNAs,and sugars to photoaffinity ligands2006

    • Author(s)
      Y.sadakane, M.Kaneda, H.Nakashima, T.Tomohiro, K.Konoha, M.Kawahara, Y.Hatanaka
    • Journal Title

      Photomedicine and Photobiology 28

      Pages: 11-12

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] Photochemical fishing approaches for identifying target proteins and elucidating the structure of a ligand-binding region using carbene-gene rating photoreactive probes2006

    • Author(s)
      Y.Sadakane, Y.Hatanaka
    • Journal Title

      Anal.Sci. 22

      Pages: 209-218

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
    • Peer Reviewed
  • [Journal Article] Isolation of novel cathepsin B inhibitory peptides from enzymatic hydrolysates of human proteins2006

    • Author(s)
      H.Koyama;M.Yasuda, K.Mawatari, K.Kaneko, Y.Sadakane, Y.Hatanaka, K.Nakagomi
    • Journal Title

      Peptide Sciences 43

      Pages: 313-314

    • NAID

      10020530123

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
    • Peer Reviewed
  • [Journal Article] Analysis of Structural Alternations of L-Asp and L-Asn Residues in Peptides Related to Neuronal Disease2006

    • Author(s)
      Y.Sadakane, S.Kumamoto, K.Konoha, M.Kawahara, K.Nakagomi
    • Journal Title

      Peptide Sciences 43

      Pages: 284-284

    • NAID

      10020529985

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
    • Peer Reviewed
  • [Journal Article] Carnosine protects GT1-7 cell against zinc-induced neurotoxicity:a possible candidate for treatment for vascular type of dementia2006

    • Author(s)
      K.Konoha, Y.Sadakane, M.Kawahara
    • Journal Title

      Trace Nutrients Research 23

      Pages: 1-8

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
    • Peer Reviewed
  • [Journal Article] Zinc neurotoxicity and its role in neurodegenerative diseases2006

    • Author(s)
      K.Konoha, Y.Sadakane, M.Kawahara
    • Journal Title

      J.Health Sci. 52

      Pages: 1-8

    • NAID

      110006163207

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
    • Peer Reviewed
  • [Journal Article] Kinetic study of racemization of aspartyl residues in recombinant human α A-crystallin2006

    • Author(s)
      T Nakamura, Y Sadakane, N.Fujii
    • Journal Title

      BBA-Proteins and Proteomics 1764

      Pages: 800-806

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
    • Peer Reviewed
  • [Journal Article] Pore formation and distuption of calciumhomeostasis by amyloidogenic peptides including Alzheimer's beta-amyloid protein,prion protein fragment peptide, and alpha-synuclein fragment2006

    • Author(s)
      K.Konoha, Y.Sadakane, M.Kawahara
    • Journal Title

      Alzheimer's Disease:New Advances

      Pages: 323-327

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
    • Peer Reviewed
  • [Journal Article] Simple and Versatile Method for Tagging Phenyldiazirine Photophores.2006

    • Author(s)
      H. Nakashima, M. Hashimoto, Y. Sadakane, T. Tomohiro, Y. Hatanaka
    • Journal Title

      J Am. Chem. Soc.(with peer review) 128

      Pages: 15092-15093

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] Hatanaka : Phenyldiazirine unit for converting peptides, proteins, DNAs, and sugars to photoaffinity ligands2006

    • Author(s)
      Y. Sadakane, M. Kaneda, H. Nakashima, T. Tomohiro, K. Konoha, M. Kawahara, Y. Hatanaka
    • Journal Title

      Photomedicine and Photobiology(without peer review.) 28

      Pages: 11-12

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] Photochemical fishing approaches for identifying target proteins and elucidating the structure of a ligand-binding region using carbene-generating photoreactive probes.2006

    • Author(s)
      Y. Sadakane, Y. Hatanaka
    • Journal Title

      Anal. Sci.(with peer review.) 22

      Pages: 109-218

    • NAID

      10017246234

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] Isolation of novel cathepsin B inhibitory peptides from enzymatic hydrolysates of human proteins.2006

    • Author(s)
      H. Koyama ; M. Yasuda, K. Mawatari, K. Kaneko, Y. Sadakane, Y. Hatanaka, K. Nakagomi
    • Journal Title

      Peptide Sciences.(with peer review.) 43

      Pages: 313-314

    • NAID

      10020530123

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] Analysis of Structural Alternations of L-Asp and L-Asn Residues in Peptides Related to Neuronal Disease.2006

    • Author(s)
      Y. Sadakane, S. Kumamoto, K. Konoha, M. Kawahara, K. Nakagomi
    • Journal Title

      Peptide Sciences.(with peer review.) 43

      Pages: 284-284

    • NAID

      10020529985

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] Carnosine protects GT 1-7 cell against zinc-induced neurotoxicity : a possible candidate for treatment for vascular type of dementia.2006

    • Author(s)
      K. Konoha, Y. Sadakane, M. Kawahara
    • Journal Title

      Trace Nutrients Research, (with peer review.) 23

      Pages: 1-8

    • NAID

      40015330447

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] Zinc neurotoxicity and its role in neurodegenerative diseases2006

    • Author(s)
      K. Konoha, Y. Sadakane, M. Kawahara
    • Journal Title

      J. Health Sd.(with peer review.) 52

      Pages: 1-8

    • NAID

      110006163207

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] Kinetic study of racemization of aspartyl residues in recombinant human αA-crystallin.2006

    • Author(s)
      T Nakamura, Y Sadakane, N. Fujii
    • Journal Title

      BBA - Proteins and Proteomics, (with peer review.) 1764

      Pages: 800-806

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] Pore formation and distuption of calciumhomeostasis by amyloidogenic peptidesincluding Alzheimer's beta-amyloid protein, prion protein fragment peptide, and aipha-synuclein fragment.2006

    • Author(s)
      K. Konoha, Y. Sadakane, M. Kawahara
    • Journal Title

      Alzheimer r Disease : New Advances(with peer review.)

      Pages: 323-327

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] Photochemical fishing approaches for identifying target proteins and elucidating the structure of a ligand-binding region using carbene-generating photoreactive probes2006

    • Author(s)
      Y.Sadakane, Y.Hatanaka
    • Journal Title

      Anal.Sci. 22

      Pages: 209-218

    • NAID

      10017246234

    • Related Report
      2006 Annual Research Report
  • [Journal Article] Photoaffinity labeling of opioid-binding cell-adhesion molecule(OBCAM) by phenyldiazirine-integrated ligand.2006

    • Author(s)
      Y.sadakane, E.Kitamoto, T.Tomohiro, K.Konoha, M.Kawahara, Y.Hatanaka
    • Journal Title

      J.KUHW 8

      Pages: 179-185

    • NAID

      110006407510

    • Related Report
      2006 Annual Research Report
  • [Journal Article] Analysis of Structural Alternations of L-Asp and L-Asn Residues in Peptides Related to Neuronal Disease.2006

    • Author(s)
      Y.Sadakane, S.Kumamoto, K.Konoda, M.Kawahara, K.Nakagomi
    • Journal Title

      Peptide Sciences 43

      Pages: 284-284

    • NAID

      10020529985

    • Related Report
      2006 Annual Research Report
  • [Journal Article] Carnosine protects GT1-7 cell against zinc-induced neurotoxicity : a possible candidate for treatment for vascular type of dementia.2006

    • Author(s)
      K.Konoha, Y.Sadakane, M.Kawahara
    • Journal Title

      Trace Nutrients Research 23

      Pages: 1-8

    • NAID

      40015330447

    • Related Report
      2006 Annual Research Report
  • [Journal Article] Kinetic study of racemization of aspartyl residues in recombinant human α A-crystallin.2006

    • Author(s)
      T Nakamura, Y Sadakane, N. Fujii
    • Journal Title

      BBA-Proteins and Proteomics 1764

      Pages: 800-806

    • Related Report
      2006 Annual Research Report
  • [Journal Article] Y. Hatanaka : Photoaffinity labeling of opioid-binding cell-adhesion molecule(OBCAM) by phenyldiazirine-integrated ligand.2005

    • Author(s)
      Y. Sadakane, E. Kitamoto, T. Tomohiro, K. Konoha, M. Kawahara, Y. Hatanaka
    • Journal Title

      J. KUHW(without peer review.) 8

      Pages: 179-185

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] 亜鉛による神経細胞死:クエン酸の抑制活性とそのメカニズムについて2008

    • Author(s)
      定金 豊, 木葉 敬子, 永田 哲也, 川原 正博
    • Organizer
      日本薬学会第128年会
    • Place of Presentation
      横浜
    • Year and Date
      2008-03-27
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] 亜鉛による培養神経細胞死に対する金属イオンの阻害メカニズムの検討2008

    • Author(s)
      木葉 敬子, 永田 哲也, 定金 豊, 川原 正博
    • Organizer
      日本薬学会第128年会
    • Place of Presentation
      横浜
    • Year and Date
      2008-03-27
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] プリオン蛋白による培養神経細胞に対する毒性メカニズムの検討2008

    • Author(s)
      永田 哲也, 定金 豊, 木葉 敬子, 川原 正博
    • Organizer
      日本薬学会第128年会
    • Place of Presentation
      横浜
    • Year and Date
      2008-03-27
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] 光反応基ジアジリンを利用した生化学的手法の開拓2008

    • Author(s)
      定金 豊, 畑中 保丸
    • Organizer
      日本薬学会第128年会
    • Place of Presentation
      横浜
    • Year and Date
      2008-03-27
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] Zinc-induced neuronal cell death : inhibitory activity of citric acid and its Zinc-inducedmechanism.2008

    • Author(s)
      Y. Sadakane, K. Konoha, T. Nagata, M.Kawahara
    • Organizer
      128th Annual Meeting of the Pharmaceutical Society of Japan
    • Place of Presentation
      Yokohama, Japan
    • Year and Date
      2008-03-27
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] Inhibitory mechanism of metal ionsonzinc-induced neurotoxicity of culturedneuronal cells.2008

    • Author(s)
      K. Konoha, Y. Sadakane, T. Nagata, M. Kawahara
    • Organizer
      128th Annual Meeting of the Pharmaceutical Society of Japan
    • Place of Presentation
      Yokohama, Japan
    • Year and Date
      2008-03-27
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] Molecular mechanism of neurotoxicity induced by prion protein peptide.2008

    • Author(s)
      T. Nagata, Y.Sadakane, K. Konoha, M.Kawahara
    • Organizer
      128th Annual Meeting of the Pharmaceutical Society of Japan
    • Place of Presentation
      Yokohama, Japan
    • Year and Date
      2008-03-27
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] Endeavors to make the photophore, diazirine easy to use : Anapplication to biochemical methodology.2008

    • Author(s)
      Y. Sadakane, Y. Hatanaka
    • Organizer
      128th Annual Meeting of the Pharmaceutical Society of Japan
    • Place of Presentation
      Yokohama, Japan
    • Year and Date
      2008-03-27
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] 光反応基ジアジリンを利用した生化学的手法の開拓2008

    • Author(s)
      定金 豊、畑中 保丸
    • Organizer
      日本薬学会128年会シンポジウム(招待講演)
    • Place of Presentation
      横浜・パシフィコ横浜
    • Year and Date
      2008-03-27
    • Related Report
      2007 Annual Research Report
  • [Presentation] クエン酸による神経細胞死抑制活性とその作用機構の解明2007

    • Author(s)
      定金 豊, 木葉 敬子, 永田 哲也, 川原 正博
    • Organizer
      第24回日本薬学会九州支部大会
    • Place of Presentation
      福岡
    • Year and Date
      2007-12-14
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] Investigation of the mechanism of zinc-induced neuronal cell death by using citrate.2007

    • Author(s)
      Y. Sadakane, K. Konoha, T. Nagata, M. Kawahara
    • Organizer
      24th Annual Meeting of Kyushu branch of the Pharmaceutical Society of Japan
    • Place of Presentation
      Fukuoka, Japan
    • Year and Date
      2007-12-14
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] ヒトαB-クリスタリン変異体中のAsp36およびAsp62残基のラセミ化反応速度の解析と構造活性相関2007

    • Author(s)
      中村 徹, 酒井 美世, 定金 豊, 芳賀 達也, 後藤 祐児, 木野内 忠稔, 佐藤 剛, 藤井 紀子
    • Organizer
      第3回D-アミノ酸研究会
    • Place of Presentation
      徳島
    • Year and Date
      2007-09-14
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] L-β-アスパラギン酸含有ペプチドの逆相HPLC定量法によるアスパラギン酸残基の異性化速度の解析:タンパク質の高次構造との関係2007

    • Author(s)
      定金 豊, 木葉 敬子, 永田 哲也, 川原 正博, 中込 和哉, 藤井 紀子
    • Organizer
      第3回D-アミノ酸研究会
    • Place of Presentation
      徳島
    • Year and Date
      2007-09-14
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] Kinetic analysis of rate constants of racemization at Asp36 and Asp62 residues in human alpha B-crystallin mutants, and their structure-function relationship.2007

    • Author(s)
      T. Nakamura, M. Sakai, Y. Sadakane, T. Haga, Y. Goto, T. Kinouchi, T. Sato, N. Fujii
    • Organizer
      3rd D-amino acid research meeting
    • Place of Presentation
      Tokushima, Japan
    • Year and Date
      2007-09-14
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] Analysis of alternation of L-Asp residues in recombinant human alpha-crystallin by reversed-phase HPLC.2007

    • Author(s)
      Y. Sadakane, K. Konoha, T. Nagata, M. Kawahara, K. Nakagomi, N. Fujii
    • Organizer
      3rd D-amino acid research meeting
    • Place of Presentation
      Tokushima, Japan
    • Year and Date
      2007-09-14
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] アルミニウムのラット大脳皮質培養神経細胞に対する毒性の化学形態による比較2007

    • Author(s)
      木葉 敬子, 永田 哲也, 定金 豊, 川原 正博
    • Organizer
      第18回日本微量元素学会
    • Place of Presentation
      福井
    • Year and Date
      2007-07-05
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] Structural comparison of Aluminum-toxicity for rat primary culture cell of cerebral cortex.2007

    • Author(s)
      K. Konoha, T. Nagata, Y. Sadakane, M. Kawahara
    • Organizer
      18th Japan Society for Biomedical Research on Trace Elements
    • Place of Presentation
      Fukui, Japan
    • Year and Date
      2007-07-05
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] 亜鉛による培養神経細胞死メカニズムの検討2007

    • Author(s)
      木葉 敬子, 永田 哲也, 定金 豊, 川原 正博
    • Organizer
      第17回金属の関与する生体関連反応シンポジウム
    • Place of Presentation
      京都
    • Year and Date
      2007-06-21
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] Mechanism for zinc-induced neuronal cell death.2007

    • Author(s)
      K. Konoha, T. Nagata, Y. Sadakane, M. Kawahara
    • Organizer
      17th Symposium on Role of Metals in Biological Reactions, Biology and Medicine
    • Place of Presentation
      Kyoto, Japan
    • Year and Date
      2007-06-21
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] 農産物・魚介類中における脳血管性認知症予防物質の探索と単離,構造解析2007

    • Author(s)
      川原 正博, 木葉 敬子, 永田 哲也, 定金 豊
    • Organizer
      第24回微量栄養素研究会シンポジウム
    • Place of Presentation
      京都
    • Year and Date
      2007-06-07
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] Purification and structural determination of the suppression substance for cerebrovascular dementia in farm products and seafoods.2007

    • Author(s)
      M. Kawahara, K. Konoha, T. Nagata, Y. Sadakane
    • Organizer
      24th Symposium of Trace Nutrients Research
    • Place of Presentation
      Kyoto, Japan
    • Year and Date
      2007-06-07
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] カルノシンによる神経細胞死抑制活性とその作用機構の解明2007

    • Author(s)
      定金 豊, 木葉 敬子, 川原 正博
    • Organizer
      日本薬学会第127年会
    • Place of Presentation
      富山
    • Year and Date
      2007-03-28
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] マンゴー(Mangifera indica L.)果実抽出液の神経細胞死抑制活性成分の同定2007

    • Author(s)
      木葉 敬子, 定金 豊, 川原 正博
    • Organizer
      日本薬学会第127年会
    • Place of Presentation
      富山
    • Year and Date
      2007-03-28
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] レトロインバルソ型カテプシンB阻害ペプチドの合成2007

    • Author(s)
      小山 裕也, 若宮 哲郎, 安田 誠, 馬渡 健一, 金子 希代子, 中込 和哉, 箕浦 憲彦, 定金 豊, 畑中 保丸
    • Organizer
      日本薬学会第127年会
    • Place of Presentation
      富山
    • Year and Date
      2007-03-28
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] 光反応性Pt-DNAによる結合タンパク質のアフィニティー解析2007

    • Author(s)
      友廣 岳則, 石原 史朗, 神宮寺 秀幸, 定金 豊, 畑中 保丸
    • Organizer
      日本薬学会第127年会
    • Place of Presentation
      富山
    • Year and Date
      2007-03-28
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] Inhibitory activity and mechanisms of carnosine against the zinc-induced neurotoxicity of immortalized hypothalamic neurons.2007

    • Author(s)
      Y. Sadakane, K. Konoha, M. Kawahara
    • Organizer
      127th Annual Meeting of the Pharmaceutical Society of apan
    • Place of Presentation
      Toyama, Japan
    • Year and Date
      2007-03-28
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] Characterization of inhibitory compounds in Mangifera indica L.fruit extract, which suppress the zinc-induced neurotoxicity of immortalized hypothalamic neurons.2007

    • Author(s)
      K. Konoha, Y. Sadakane, M. Kawahara
    • Organizer
      127th Annual Meeting of the Pharmaceutical Society of apan
    • Place of Presentation
      Toyama, Japan
    • Year and Date
      2007-03-28
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] Synthesis of Cathepsinn B inhibitory retro-inverso peptidyl analogues.2007

    • Author(s)
      Y. Koyama, T. Wakamiya, M. Yasuda, K. Mawatari, K. Kaneko, K. Kazuya, N. Minoura, Y. Sadakane, Y. Hatanaka
    • Organizer
      127th Annual Meeting of the Pharmaceutical Society of apan
    • Place of Presentation
      Toyama, Japan
    • Year and Date
      2007-03-28
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] Photoaffinity-Based Binding Analysis of CDDP-Damaged DNA-Protein Complex.2007

    • Author(s)
      T. Tomohiro, S. Ishihara, H. Jinguji, Y. Sadakane, Y. Hatanaka
    • Organizer
      127th Annual Meeting of the Pharmaceutical Society of apan
    • Place of Presentation
      Toyama, Japan
    • Year and Date
      2007-03-28
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] Zinc-induced apoptosis of GT1-7 cells are protected by mango(Mangifera indica L.)fruit:a candidate for treatment of neurodegeneration after ischemia2007

    • Author(s)
      M.Kawahara, K.Konoha, T.Nagata, Y.Sadakane
    • Organizer
      Neuroscience 2007
    • Place of Presentation
      San Diego,USA
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] Zinc-induced apoptosis of GT1-7 cells are protected by mango(Mangifera indica L.) fruit : a candidate for treatment of neurodegeneration after ischemia.2007

    • Author(s)
      M. Kawahara, K. Konoha, T. Nagata, Y. Sadakane
    • Organizer
      Neuroscience 2007
    • Place of Presentation
      San Diego, USA
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] 亜鉛神経毒性を指標としたスクリーニングによる脳血管性認知症予防物質の探索2006

    • Author(s)
      定金 豊, 木葉 敬子, 川原 正博
    • Organizer
      第23回日本薬学会九州支部大会
    • Place of Presentation
      熊本
    • Year and Date
      2006-12-09
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] 光反応基ジアジリンを利用した生物学的手法の開発2006

    • Author(s)
      定金 豊
    • Organizer
      第23回日本薬学会九州支部大会
    • Place of Presentation
      熊本
    • Year and Date
      2006-12-09
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] Screening with zinc-induced neuronal degradation for the substances for suppression of cerebrovascular dementia.2006

    • Author(s)
      Y. Sadakane, K. Konoha, M. Kawahara
    • Organizer
      23rd Annual Meeting of Kyushu branch of the Pharmaceutical Society of Japan
    • Place of Presentation
      Kumamoto, Japan
    • Year and Date
      2006-12-09
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] Development of biological methods using photoreactive group, diazirine.2006

    • Author(s)
      Y. Sadakane
    • Organizer
      23rd Annual Meeting of Kyushu branch of the Pharmaceutical Society of Japan
    • Place of Presentation
      Kumamoto, Japan
    • Year and Date
      2006-12-09
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] 脳血管性認知症の予防物質の探索:マンゴーとウナギの有用性2006

    • Author(s)
      定金 豊, 木葉 敬子, 川原 正博
    • Organizer
      第6回OUSフオーラム2006
    • Place of Presentation
      岡山
    • Year and Date
      2006-11-24
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] Screening of suppression substance for cerebrovascular dementia : usefulness of mango fruit and eel.2006

    • Author(s)
      Y. Sadakane, K. Konoha, M. Kawahara
    • Organizer
      6th OUS Forum 2006
    • Place of Presentation
      Okayama, Japan
    • Year and Date
      2006-11-24
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] L-AspとL-Asn残基の構造変化分析手法の開発:コンフォメーション病に関わるペプチドの分析2006

    • Author(s)
      定金 豊, 木葉 敬子, 川原 正博, 中込 和哉
    • Organizer
      第2回D-アミノ酸研究会
    • Place of Presentation
      京都
    • Year and Date
      2006-09-08
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] Development of analytical method for structure alternation of L-Asp and L-Asn : peptides concerned with conformation disease.2006

    • Author(s)
      Y. Sadakane, K. Konoha, M. Kawahara, K. Nakagomi
    • Organizer
      2nd D-amino acid research meeting
    • Place of Presentation
      Kyoto, Japan
    • Year and Date
      2006-09-08
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] 海産物由来ペプチドの分析と脳血管性認知症予防物質のスクリーニング2006

    • Author(s)
      木葉 敬子, 定金 豊, 川原 正博
    • Organizer
      第19回バイオメディカル分析科学シンポジウム
    • Place of Presentation
      福岡
    • Year and Date
      2006-08-02
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] コンフォメーション病に関わるペプチドでのL-AspとL-Asn残基の構造変化分析手法2006

    • Author(s)
      定金 豊, 木葉 敬子, 川原 正博, 中込 和哉
    • Organizer
      第19回バイオメディカル分析科学シンポジウム
    • Place of Presentation
      福岡
    • Year and Date
      2006-08-02
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] Screening of the substances for suppression of cerebrovascular dementia and analysis for the peptide from seafood.2006

    • Author(s)
      K. Konoha, Y. Sadakane, M. Kawahara
    • Organizer
      19th Biomedical analytical Science Symposium
    • Place of Presentation
      Fukuoka, Japan
    • Year and Date
      2006-08-02
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] Analytical method for structure alternation of L-Asp and L-Asn in the peptides concerned with conformation disease.2006

    • Author(s)
      Y. Sadakane, K. Konoha, M. Kawahara, K. Nakagomi
    • Organizer
      19th Biomedical analytical Science Symposium
    • Place of Presentation
      Fukuoka, Japan
    • Year and Date
      2006-08-02
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] Pore formation and distuption of calciumhomeostasis by amyloidogenic peptides including Alzheimer's beta-amyloid protein, prion protein fragment peptide, and alpha-synuclein fragment.2006

    • Author(s)
      K. Konoha, Y. Sadakane, M. Kawahara
    • Organizer
      The 10th International Conference on Alzheimer's Disease and Related Disorders
    • Place of Presentation
      Madrid, Spain
    • Year and Date
      2006-07-28
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] 光反応基ジアジリンによる分子生物学的手法の開拓2006

    • Author(s)
      定金 豊, 畑中 保丸
    • Organizer
      第28回日本光医学・光生物学会
    • Place of Presentation
      徳島
    • Year and Date
      2006-07-07
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] Endeavor for methods in molecular biology with photoreactive group, diazirine.2006

    • Author(s)
      Y. Sadakane, Y. Hatanaka
    • Organizer
      28th Japan photomedical and photobiology
    • Place of Presentation
      Tokushima, Japan
    • Year and Date
      2006-07-07
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] 亜鉛神経毒性スクリーニングによる脳血管性認知症予防物質の探索2006

    • Author(s)
      木葉 敬子, 定金 豊, 川原 正博
    • Organizer
      第23回微量栄養素研究会シンポジウム
    • Place of Presentation
      京都
    • Year and Date
      2006-06-16
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] Screening of the substances for suppression of cerebrovascular dementia using zinc-induced neuronal degradation.2006

    • Author(s)
      K. Konoha, Y. Sadakane, M. Kawahara
    • Organizer
      23rd Symposium of Trace Nutrients Research
    • Place of Presentation
      Kyoto, Japan
    • Year and Date
      2006-06-16
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] マンゴー果実抽出液が神経細胞の自殺をくいとめる〜未熟果実の利用法2006

    • Author(s)
      定金 豊, 木葉 敬子, 川原 正博
    • Organizer
      第5回産学官連携推進会議
    • Place of Presentation
      京都
    • Year and Date
      2006-06-10
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] Inhibition of neuronal apoptosis by mango fruits-utilization of immature fruit-2006

    • Author(s)
      Y. Sadakane, K. Konoha, M. Kawahara
    • Organizer
      5th Congress of innovation with Academia, Industry and Government
    • Place of Presentation
      Kyoto, Japan
    • Year and Date
      2006-06-10
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] Chemical Getaway for Profiling DNA Binding Proteins2006

    • Author(s)
      Y.Hatanaka, Y.Sadakane, T.Takagi, and T.Tomohiro
    • Organizer
      18th International Symposium on Chemistry, Biology and Medicine
    • Place of Presentation
      Cyprus
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] Pore formation and distuption of calciumhomeostasis by amyloidogenic peptides including Alzheimer's beta-amyloid protein,prion protein fragment peptide,and alpha-synuclein fragment2006

    • Author(s)
      K.Konoha, Y.Sadakane, M.Kawahara
    • Organizer
      The 10th International Conference on Alzheimer's Disease and Related Disorders
    • Place of Presentation
      Madrid,Spain
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] Carnosine protects against zinc-induced apoptosis of GT1-7 cells and cultured rat hippocampal neurons:a candidate for the drugs for neurode generation after ischemia2006

    • Author(s)
      K.Konoha, Y.Sadakane, M.Kawahara
    • Organizer
      Neuroscience 2006
    • Place of Presentation
      Atlanta,USA
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] Photoaffinity-Based Analysis of Proteins Complexes Related to Cisplatin-Damaged DNA2006

    • Author(s)
      T.Tomohiro, H.Jinguji, F.Ishihara, Y.Sadakane and Y.Hatanaka
    • Organizer
      3rd Asian Biological Inorganic Chemistry Conference
    • Place of Presentation
      Nanjing,China
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] Analysis of Structural Alternations of L-Asp and L-Asn Residues in Peptides Related to Neuronal Disease2006

    • Author(s)
      Y.Sadakane, S.Kumamoto, K.Konoha, M.Kawahara, K.Nakagomi
    • Organizer
      43JPS/PEM4
    • Place of Presentation
      Yokohama,Japan
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] Chemical Getaway for Profiling DNA Binding Proteins.2006

    • Author(s)
      Y. Hatanaka, Y. Sadakane, T. Takagi, T. Tomohiro
    • Organizer
      18th International Symposium on Chemistry, Biology and Medicine
    • Place of Presentation
      Cyprus
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] Carnosine protects against zinc-induced apoptosis of GT1-7 cells and cultured rat hippocampal neurons : a candidate for the drugs for neurodegeneration after ischemia.2006

    • Author(s)
      K. Konoha Y. Sadakane, M. Kawahara
    • Organizer
      Neuroscience 2006
    • Place of Presentation
      Atlanta, USA
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] Photoaffinity-Based Analysis of Proteins Complexes Related to Cisplatin-Damaged DNA.2006

    • Author(s)
      T. Tomohiro, H. Jinguji, F. Ishihara, Y. Sadakane and Y. Hatanaka
    • Organizer
      3rd Asian Biological Inorganic Chemistry Conference
    • Place of Presentation
      Nanjing, China
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] Analysis of Structural Alternations of L-Asp and L-Asn Residues in Peptides Related to Neuronal Disease.2006

    • Author(s)
      Y. Sadakane, S. Kumamoto, K. Konoha, M. Kawahara, K. Nakagomi
    • Organizer
      43JPS/PEM4
    • Place of Presentation
      Yokohama, Japan
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Remarks] 「研究成果報告書概要(和文)」より

    • URL

      http://www.d4.dion.ne.jp/~sadafami/

    • Related Report
      2007 Final Research Report Summary
  • [Remarks]

    • URL

      http://www.d4.dion.ne.jp/~sadafami/

    • Related Report
      2007 Annual Research Report
  • [Patent(Industrial Property Rights)] 光反応基を利用したパニング法およびそれに用いるキット(PANNING METHOD UTILIZING PHOTOREACTIVE GROUP AND KIT FOR THE METHOD)2007

    • Inventor(s)
      畑中 保丸, 定金 豊
    • Industrial Property Rights Holder
      国立大学法人富山大学
    • Filing Date
      2007-01-22
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
    • Overseas
  • [Patent(Industrial Property Rights)] 光反応基を利用したパニング法およびそれに用いるキット2007

    • Inventor(s)
      畑中保丸 定金豊
    • Industrial Property Rights Holder
      国立大学法人富山大学
    • Filing Date
      2007-01-22
    • Related Report
      2006 Annual Research Report
  • [Patent(Industrial Property Rights)] NUCLEIC ACID DERIVATIVES HAVING PHENYLDIAZIR IDINE MOIETIES AND PROCESS FOR PRODUCTION THEREOF, NUCLEOTIDE DERIVATIVES HAVING PHENYLD IAZIRIDINE MOIETIES AND PROCESS FOR PRODUCTI ON THEREOF, METHOD FOR ANALYSIS OF PROTEIN, AND PROCESS FOR PREPAR ATION OF2006

    • Inventor(s)
      Yasumaru HATANAKA, Yutaka SADAKANE, Masaki, KANEDA
    • Industrial Property Rights Holder
      National University Corporation University of Toyama
    • Filing Date
      2006-05-08
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
    • Overseas

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Published: 2006-04-01   Modified: 2016-04-21  

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