| Project/Area Number |
18590118
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Environmental pharmacy
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| Research Institution | Nihon Pharmaceutical University |
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Principal Investigator |
KITAMURA Shigeyuki Nihon Pharmaceutical University, School of Pharmacy, Professor (40136057)
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| Co-Investigator(Kenkyū-buntansha) |
FUJIMOTO Nariaki Hiroshima University, Reseanch Institute for Radiation Biology and Medicine, Associate Professor (40243612)
KASHIWAGI Akihiko Hiroshima University, Graduate School of Sciences, Professor (50106796)
OHTA Shigeru Hiroshima University, Graduate Shool of Biomedical Scienoes, Professor (60160503)
SUGIHARA Kazumi Hiroshima University, Graduate Shool of Biomedical Sciences, Research Associate (20271067)
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| Project Period (FY) |
2006 – 2007
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| Project Status |
Completed (Fiscal Year 2007)
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| Budget Amount *help |
¥3,680,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥180,000)
Fiscal Year 2007: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000)
Fiscal Year 2006: ¥2,900,000 (Direct Cost: ¥2,900,000)
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| Keywords | Endocrine disruptor / Brominated flame retardant / Tetrabromobisphenol A / Polybromodiphenyl ether / Thyroid hormonal activity / Estrogenic activity / Metabolic activation / Amphibian metamorphosis / ブロム化難燃剤 / ポリブロモジフェニールエーテル / 甲状腺ホルモン / 内分泌撹乱作用 |
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| Research Abstract |
Endocrine-disrupting activity of some brominated flame retardants was examined. 1) The thyroid hormone-disrupting activity of tetrabromobisphenol A, tetrachlorobisphenol A, some 4-hydroxylated polybromodiphenyl ethers was observed in the assay of binding to thyroid hormone receptor. These chemicals also showed thyroid hormonal effect on GH3 cell. However, polybrominated diphenyl ethers and bisphenol A did not exhibit the activity. Especially, tetrabromobisphenol A exhibited the highest thyroid hormonal activity. 3, 5-Substituted bromide is an essential factor for the thyroid hormonal activity. 2) 4-Hydroxy-2, 2', 4-tribromodiphenyl ether exhibited estrogenic activity in the reporter assay using human breast cancer cell line MCF-7. In this case, 3, 5-dibromo substitution decreased the estrogenic activity. 3) The action of these chemicals was also estimated by amphibian metamorphosis using tadpole. The tail of tadopole was shortened by the addition of triiodothyronine (T3). These chemicals showed antagonistic effect against the T3-induced tadpole tail regression. These facts suggest that hydroxylated brominated flame retardants exhibited endocrine-disrupting effect in the worldwide life.
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