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Development of a clinical application of Cordyceps sinensis as an antimetastatic agent utilizing the properties of its active ingredient

Research Project

Project/Area Number 18590127
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Environmental pharmacy
Research InstitutionMukogawa Women's University

Principal Investigator

NAKAMURA Kazuki  Mukogawa Women's University, Department of Pharmacy, Professor Associate (20299093)

Project Period (FY) 2006 – 2007
Project Status Completed (Fiscal Year 2007)
Budget Amount *help
¥2,800,000 (Direct Cost: ¥2,500,000、Indirect Cost: ¥300,000)
Fiscal Year 2007: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2006: ¥1,500,000 (Direct Cost: ¥1,500,000)
KeywordsCordyceps sinensis / cordycepin / adenosine cleaminase inhibitor / mouse B16-B16 melanoma cells / spontaneous metastatic model / survival / マウスメラノーマB16-BL6 / 高転移性がん細胞
Research Abstract

We demonstrated that cordycepin (3'-deoxyadenosine) is an active ingredient of water extracts of the fruiting bodies of cultured Cordyceps sinensis (WECS). In the first year of this project, I investigated the adjuvant effect of 2'-deoxycoformycin (DCF), an adenosine deaminase inhibitor, on the antitumor actions of WECS and cordycepin using mouse B16-BL6 melanoma and Lewis lung carcinoma cells in vitro. As a result, DCF at 5 μM reinforced the antitumor effect of WECS and cordycepin, three and three hundred-fold, respectively. DCF alone at 5 μM was wholly ineffective on the growth curves of both cell lines. In the second year of this project, we evaluated the antimetastatic effect of WECS on a spontaneous metastatic mouse model, prepared by inoculation with B16-BL6 cells into the footpad of the right hind leg. Two weeks after inoculation, the primary tumor was completely resected. DCF (0.05 mg/kg) alone or DCF plus WECS (10 mg/kg) administered intraperitoneally for one week after tumor inoculation and for one week after primary tumor resection did not prolong the survival of model mice, while WECS (10 mg/kg) alone administered intraperitoneally for the same, duration significantly prolonged mouse survival compared with that of control mice, as shown on Kaplan-Meier analysis. Survival time of control mice was less than 53 days in all cases, while three of six mice that were administered WECS (10 mg/kg) survived over 110 days. None of the mice administered WECS (10 mg/kg) showed adverse systemic effects. WECS at 3 mg/kg did not show any antimetastatic action, while WECS at 100 mg/kg significantly reduced the body weights of mice as an adverse effect. In conclusion, after removing its inactive components, WECS should be a candidate for clinical use as an antimetastatic agent.

Report

(3 results)
  • 2007 Annual Research Report   Final Research Report Summary
  • 2006 Annual Research Report
  • Research Products

    (26 results)

All 2008 2007 2006 Other

All Journal Article (9 results) (of which Peer Reviewed: 4 results) Presentation (14 results) Book (1 results) Remarks (2 results)

  • [Journal Article] Cordycepin(3'-deoxyadenosine) inhibits the growth of B16-BL6 mouse melanoma cells through the stimulation of adenosine A_3 receptor followed by glycogen synthase kinase-3 β activation and cyclin D_1 suppression2008

    • Author(s)
      Noriko Yoshikawa, Kazuki Nakamura
    • Journal Title

      Naunyn Schmiedebergs Arch Pharmacol (in press)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
    • Peer Reviewed
  • [Journal Article] Cordycepin(3'-deoxyadenosine) inhibits the growth of B16-BL6 mouse melanoma cells through the stimulation of adenosine A_3 receptor followed by glycogen synthase kinase-3βactivation and cyclin D1 suppression2008

    • Author(s)
      Noriko Yoshikawa, Kazuki Nakamura, et. al.
    • Journal Title

      Naunyn Schmiedebergs Arch Pharmacol (in press)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] Cordycepin(3'-deoxyadenosine)inhibits the growth of B16-BL6 mouse melanoma cells through the stimulation of adenosine A_3 receptor followed by glycogen synthase kinase-3β activation and cyclin D_1 suppression2008

    • Author(s)
      Noriko Yoshikawa, Kazuki Nakamura
    • Journal Title

      Naunyn Schmiedebergs Arch Pharmacol (in press)

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Reinforcement of antitumor effect of Cordyceps sinensis by 2'-deoxycoformycin,an adenosine deaminase inhibitor2007

    • Author(s)
      Noriko Yoshikawa, Kazuki Nakamura
    • Journal Title

      In Vivo 21

      Pages: 291-296

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
    • Peer Reviewed
  • [Journal Article] Cordycepin and Cordyceps sinensis reduce the growth of human promyelocytic leukaemia cells through the Wnt signalling pathway2007

    • Author(s)
      Noriko Yoshikawa, Kazuki Nakamura
    • Journal Title

      Clin Exp Pharmacol Physiol 34

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Annual Research Report 2007 Final Research Report Summary
    • Peer Reviewed
  • [Journal Article] Reinforcement of antitumor effect of Cordyceps sinensis by 2'-deoxycoformycin, an adenosine deaminase inhibitor2007

    • Author(s)
      Noriko Yoshikawa, Kazuki Nakamura, et. al.
    • Journal Title

      In Vivo 21

      Pages: 291-296

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] Cordycepin and Cordyceps sinensis reduce the growth of human promyelocytic leukaemia cells through the Wnt signalling pathway2007

    • Author(s)
      Noriko Yoshikawa, Kazuki Nakamura, et. al.
    • Journal Title

      Clin Exp Pharmacol Physiol 34

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] Cordyceps sinensis2007

    • Author(s)
      Kazuki Nakamura
    • Journal Title

      Science Forum, Guide Book for Safety of Functional Foods

      Pages: 340-342

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] Reinforcement of Antitumor Effect of Cordyceps Sinensis by 2'-Deoxycoformycin, an Adenosine Deaminase Inhibitor2007

    • Author(s)
      Noriko Yoshikawa, Kazuki Nakamura
    • Journal Title

      in vivo 21・2

      Pages: 291-296

    • Related Report
      2006 Annual Research Report
  • [Presentation] 4種のマウスメラノーマ細胞における転移先臓器指向性の違いについて2008

    • Author(s)
      久保 えり香, 中村 一基
    • Organizer
      第81回日本薬理学会年会
    • Place of Presentation
      横浜市
    • Year and Date
      2008-03-17
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Annual Research Report 2007 Final Research Report Summary
  • [Presentation] Cordycepin(3'-deoxyadenosine)reduces the growth of B16-BL6 mouse melanoma cells through the adenosine A_3 receptor followed by Wnt signaling pathway2007

    • Author(s)
      Kazuki Nakamura
    • Organizer
      The 5th International Receptor Symposium
    • Place of Presentation
      Shizuoka,Japan
    • Year and Date
      2007-05-10
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] Cordycepin(3'-deoxyadenosine) reduces the growth of B16-BL6 mouse melanoma cells through the adenosine A_3 receptor followed by Wnt signaling pathway2007

    • Author(s)
      Kazuki Nakamura, et. al.
    • Organizer
      The 5th International Receptor Symposium
    • Place of Presentation
      Shizuoka, Japan
    • Year and Date
      2007-05-10
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] Cordycepin(3'-deoxyadenosine)reduces the growth of B 16-BL6 mouse melanomacells through the adenosine A_3 receptor followed by Wnt signaling pathway2007

    • Author(s)
      Kazuki Nakamura
    • Organizer
      The sth International Receptor Symposium
    • Place of Presentation
      Shizuoka,Japan
    • Year and Date
      2007-05-10
    • Related Report
      2007 Annual Research Report
  • [Presentation] Potential for liver metastasis did not correlate with that for lung metastasis in a study using four different mouse melanoma cell lines2007

    • Author(s)
      Erika Kubo, Noriko Yoshikawa, Kazuki Nakamura, et. al.
    • Organizer
      The 81th annual meeting of The Japanese Pharmacological Society
    • Place of Presentation
      Yokohama, Japan
    • Year and Date
      2007-03-17
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] 冬虫夏草の成分コーディセピンによるマウスメラノーマ細胞のがん転移抑制には血小板凝集抑制が関与する2007

    • Author(s)
      吉川 紀子, 中村 一基
    • Organizer
      第80回日本薬理学会年会
    • Place of Presentation
      名古屋市
    • Year and Date
      2007-03-16
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] Anti-metastatic effect of cordycepin, an ingredient of Cordyceps sinensis, is associated with inhibition of platelet aggregation induced by mouse melanoma cells2007

    • Author(s)
      Noriko Yoshikawa, Kazuki Nakamura, et. al.
    • Organizer
      The 80th annual meeting of The Japanese Pharmacological Society
    • Place of Presentation
      Nagoya, Japan
    • Year and Date
      2007-03-16
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] Cordycepin,an active ingredient of Cordyceps sinensis,reduces the growth of human promyelocytic leukemia cells2006

    • Author(s)
      Noriko Yoshikawa, Kazuki Nakamura
    • Organizer
      The 2nd International Symposium on Lifestyle Related Diseases
    • Place of Presentation
      Nishinomiya,Japan
    • Year and Date
      2006-10-22
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] Cordycepin an active ingredient of Cordyceps sinensis, reduces the growth of human promyelocytic leukemia cells2006

    • Author(s)
      Noriko Yoshikawa, Kazuki Nakamura, et. al.
    • Organizer
      The 2nd International Symposium on Lifestyle Related Diseases
    • Place of Presentation
      Nishinomiya, Japan
    • Year and Date
      2006-10-22
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] Difference in adhesive potential to substrate adhesive molecules(collagen IV,Laminin or fibronectin)among B16-F0,-F1,-F10 and -BL6 mouse melanoma cell lines2006

    • Author(s)
      Noriko Yoshikawa, Kazuki Nakamura
    • Organizer
      The 18th Japan-Korea Joint Seminar on Pharmacology
    • Place of Presentation
      Fukui,Japan
    • Year and Date
      2006-09-23
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] Difference in adhesive potential to substrate adhesive molecules(collagen IV, laminin or fibronectin) among B 16-FO,-F1,-F10 and-BL6 mouse melanoma cell lines2006

    • Author(s)
      Noriko Yoshikawa, Kazuki Nakamura, et. al.
    • Organizer
      The 18th Japan-Korea Joint Seminar on Pharmacology
    • Place of Presentation
      Fukui, Japan
    • Year and Date
      2006-09-23
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] Cordycepin,an active ingredient of Cordyceps sinensis,inhibits tumor growth by stimulating adenosine A_3 receptor2006

    • Author(s)
      Noriko Yoshikawa, Kazuki Nakamura
    • Organizer
      The 15th World Congress of Pharmacology
    • Place of Presentation
      Beijing,China
    • Year and Date
      2006-07-03
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] Cordycepin, an active ingredient of Cordyceps sinensis, inhibits tumor growth by stimulating adenosine As receptor2006

    • Author(s)
      Noriko Yoshikawa, Kazuki Nakamura, et. al.
    • Organizer
      The 15th World Congress of Pharmacology
    • Place of Presentation
      Beijing, China
    • Year and Date
      2006-07-03
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] マウスメラノーマ細胞の転移能における細胞径及びintegrin β_1タンパク発現の関与2006

    • Author(s)
      吉川 紀子, 中村 一基
    • Organizer
      第109回日本薬理学会近畿部会
    • Place of Presentation
      倉敷市
    • Year and Date
      2006-06-16
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Book] 機能性食品の安全性ガイドブック(冬虫夏草)2007

    • Author(s)
      中村 一基
    • Publisher
      サイエンスフォーラム社
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Annual Research Report 2007 Final Research Report Summary
  • [Remarks] 「研究成果報告書概要(和文)」より

    • URL

      http://ph.mukogawa-u.ac.jp/~yakuri1/

    • Related Report
      2007 Final Research Report Summary
  • [Remarks]

    • URL

      http://ph.mukogawa-u.ac.jp/~yakuri1/

    • Related Report
      2007 Annual Research Report

URL: 

Published: 2006-04-01   Modified: 2016-04-21  

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