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Research on the elucidation for the mechanism of nonspecific production of cyanide

Research Project

Project/Area Number 18590130
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Environmental pharmacy
Research InstitutionNational Research Institute of Police Science

Principal Investigator

YASUO Seto  National Research Institute of Police Science, Third Department of Forensic Science, Vice Director (10154668)

Project Period (FY) 2006 – 2007
Project Status Completed (Fiscal Year 2007)
Budget Amount *help
¥4,010,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥510,000)
Fiscal Year 2007: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
Fiscal Year 2006: ¥1,800,000 (Direct Cost: ¥1,800,000)
KeywordsCyanide / Hazardous substance / Analytical chemistry / Poisoning case / Reaction mechanism / Active oxygen / Organic chemistry / Forensic toxicolo
Research Abstract

(1) Quantification of cyanide
Static head-space gas chromatographic method was established for quantifying cyanide using porous polymer capillary column and nitrogen-phosphors detector, which enabled determination of ng/ml level of cyanide without interference of sample matrix.
(2) Production of cyanide from thiocyanate
Considerable levels of cyanide (several to several decade μM) were produced from mM levels of thiocyanate in the presence of hydrogen peroxide, hydroperoxide radical and nitrite under strong acidic condition. In contrast, under the weak acidic condition production of cyanide was not significant (less than 1 μM).
(3) Production of cyanide from beverage samples
From the 50℃ incubation mixture of the commercial instant coffee drink (Nescafe Goldbrend Original, 0.15%) and nitrite (50 mM), 1.2 and 0.4 p.g/mL cyanide was produced in the presence of 10% phosphoric acid and pH 5.0 buffer, respectively. Also from those of oolong tea, 2.9 and 0.5 μg/mL cyanide was produced. Cyanide (1.7〜3.4 mg/mL) was also produced from the reaction mixture of nitrite and natural polyphenol compounds such as chlorogenic acid, caffeic acid, catechin, epicatechin, tannic acid, gallic acid and L-epinephrine. From the synthetic polyphenols, cyanide (0.5〜6.7 μg/mL, 0.1〜15 mg/mL) was also produced. The produced amount of cyanide was dependent on the substrate concentrations of nitrite and polyphenol, pH, temperature and incubation time. Production of cyanide was ascertained by the spectrophotometric method.
(4) Conclusion
Nitrite was reacted with body-fluid thiocyanate and non-nitrogenous polyphenol compounds under acidic conditions, producing significant level of cyanide in the special conditions. Forensic toxicologists should pay attention to this type of the artifactual cyanide production.

Report

(3 results)
  • 2007 Annual Research Report   Final Research Report Summary
  • 2006 Annual Research Report
  • Research Products

    (14 results)

All 2007 2006

All Journal Article (6 results) (of which Peer Reviewed: 3 results) Presentation (8 results)

  • [Journal Article] 毒劇物中毒事件における危機管理と分析化学の役割2007

    • Author(s)
      瀬戸康雄
    • Journal Title

      分析化学 56

      Pages: 981-991

    • NAID

      110006570390

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
    • Peer Reviewed
  • [Journal Article] Analytical chemistry contributing to crisis management in poisoning cases2007

    • Author(s)
      Yasuo Seto
    • Journal Title

      Bunseki Kagaku 56

      Pages: 981-991

    • NAID

      110006570390

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] 毒劇物中毒事件における危機管理と分析化学の役割2007

    • Author(s)
      瀬戸 康雄
    • Journal Title

      分析化学 56

      Pages: 981-991

    • NAID

      110006570390

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Journal Article] 安全安心な社会を目指す危機管理分析化学2006

    • Author(s)
      瀬戸康雄
    • Journal Title

      バイオサイエンスとインダストリー 64

      Pages: 494-499

    • NAID

      10018069244

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
    • Peer Reviewed
  • [Journal Article] Analytical chemistry in crisis management aimed for realizing safe and reliable society2006

    • Author(s)
      Yasuo Seto
    • Journal Title

      Bioscience and Industry 64

      Pages: 494-499

    • NAID

      10018069244

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] 安全安心な社会を目指す危機管理分析化学2006

    • Author(s)
      瀬戸 康雄
    • Journal Title

      バイオサイエンスとインダストリー 64.9

      Pages: 494-499

    • NAID

      10018069244

    • Related Report
      2006 Annual Research Report
  • [Presentation] シアンの食品試料からの非特異的生成について2007

    • Author(s)
      瀬戸康雄
    • Organizer
      日本分析化学会第56年会
    • Place of Presentation
      徳島
    • Year and Date
      2007-09-21
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] Nonspecific production of cynide from beverage samples2007

    • Author(s)
      Yasuo Seto
    • Organizer
      56th Annual Meeting of Japanese Society for Analytical Chemistry
    • Place of Presentation
      Tokushima
    • Year and Date
      2007-09-21
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] シアンの食品試料からの非特異的生成について2007

    • Author(s)
      瀬戸 康雄, 他
    • Organizer
      日本分析化学会第56年会
    • Place of Presentation
      徳島市
    • Year and Date
      2007-09-19
    • Related Report
      2007 Annual Research Report
  • [Presentation] Pitfalls in cyanide detection in postmortem toxicology : Two case example2007

    • Author(s)
      瀬戸康雄
    • Organizer
      45th International Congress on Forensic Toxicology(TIAFT)
    • Place of Presentation
      シアトル(合衆国)
    • Year and Date
      2007-08-29
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] Pitfalls in cyanide detection in postmortem toxicology : Two case example2007

    • Author(s)
      Yasuo Seto
    • Organizer
      45th International Congress on Forensic Toxicology(TIAFT)
    • Place of Presentation
      Seattle(USA)
    • Year and Date
      2007-08-29
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] Pitfalls in cyanide deternmination in postmortem toxicology:two case examples2007

    • Author(s)
      瀬戸 康雄, 他
    • Organizer
      45th International Congress on Forensic Toxicology(TLAFT)
    • Place of Presentation
      シアトル(合衆国)
    • Year and Date
      2007-08-29
    • Related Report
      2007 Annual Research Report
  • [Presentation] 毒劇物中毒事件の危機管理に対応する分析化学(依頼講演)2007

    • Author(s)
      瀬戸康雄
    • Organizer
      第68回日本分析化学討論会
    • Place of Presentation
      宇都宮
    • Year and Date
      2007-05-19
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] Analytical chemistry contributing to crisis management in poisoning sases2007

    • Author(s)
      Yasuo Seto
    • Organizer
      68th Annual Spring Meeting of Japanese Society for Analytical Chemistry
    • Place of Presentation
      Utsunomiya
    • Year and Date
      2007-05-19
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary

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Published: 2006-04-01   Modified: 2016-04-21  

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