Influence of disease stage and pharmacotherapy on gene delivery

• Project/Area Number: 18590143
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Medical pharmacy
• Research Institution: Nagasaki University
• Principal Investigator: SASAKI Hitoshi Nagasaki University, 医学部・歯学部附属病院, 教授 (00170689)
• Co-Investigator(Kenkyū-buntansha): TO Hideto 長崎大学, 医学部・歯学部附属病院, 准教授 (90346809)
• Project Period (FY): 2006 – 2008
• Project Status: Completed (Fiscal Year 2008)
• Budget Amount: ¥3,970,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥570,000); Fiscal Year 2008: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000); Fiscal Year 2007: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2006: ¥1,500,000 (Direct Cost: ¥1,500,000)
• Keywords: 遺伝子 / デリバリー / 疾病 / 肝臓 / カチオン性高分子 / 肝炎 / 肝切除 / 遺伝子治療 / 遺伝子デリバリー / カチオン性リポソーム / ウイルス性肝炎モデル / 薬剤性肝炎モデル / ガラクトサミン / 遣伝子 / 遣伝子治療

Research Abstract

我々は代表的な遺伝子導入ベクターを用いて、疾患の病態が及ぼす遺伝子導入効率への影響を検討した。その結果、数種の疾患モデルマウスにおける遺伝子発現形態は正常マウスとは大きく異なり、疾患の病態や病期が遺伝子発現量に大きく影響することを明らかにした。また、この遺伝子発現量の変化は、疾患の種類や遺伝子導入ベクターの構成成分によって大きく異なることを見いだし、疾患毎に最適な遺伝子導入ベクターの選択が必要であることを実証した。

Research Products

• [Journal Article] Cationic liposomes-mediated plasmid DNA delivery in murine hepatitis induced by carbon tetrachloride (2009)
  • Author(s): Yoshioka T, Yoshida S, Kurosaki T, Teshima M, Nishida K, Nakamura J, Nakashima M, To H, Kitahara T, Sasaki H
  • Journal Title: J Liposome Res 23 Pages: 1-7
  • NAID: 120006984575
  • Peer Reviewed
• [Journal Article] Cationic liposomes-mediated plasmid DNA delivery in murine hepatitis induced by carbon tetrachloride. (2009)
  • Author(s): Yoshioka T, et al.
  • Journal Title: J Liposome Res. 23 Pages: 1-7
  • Peer Reviewed
• [Journal Article] Influence of murine hepatitis induced by D-(+)-galactosamine hydrochloride and lipopolysaccharide on gene expression of polyethylenimine/plasmid DNA polyplex (2008)
  • Author(s): Miyanaga K, Yoshioka T, Nakagawa H, Kitahara T, To H, Ichikawa N, Nakashima M, Nishida K, Nakamura J, Sasaki H
  • Journal Title: Biol Pharm Bull 31 Pages: 1585-9
  • NAID: 110006839022
  • Peer Reviewed
• [Journal Article] Influence of murine hepatitis induced by D-(+)-galactos amine hydrochloride and lipopolysaccharide on gene expression of polyethylenimihe/plasmid DNA polyplex. (2008)
  • Author(s): Miyanaga K, et al.
  • Journal Title: Biol Pharm Bull. 31 Pages: 1585-9
  • Peer Reviewed
• [Journal Article] Spleen-selective gene transfer following the administration of naked plasmid DNA onto the spleen surface in mice (2007)
  • Author(s): J.Nakamura, S.Fumoto, R.Kawanami, H.Sasaki, et. al.
  • Journal Title: Biol.Pharm.Bull. 30 Pages: 941-945
  • NAID: 110006273294
  • Peer Reviewed
• [Journal Article] Evaluation of enhanced peritoneum permeability in methylglyoxal-treated rats as a diagnostic method for peritoneal damage (2007)
  • Author(s): S.Fumoto, Y.Nakashima, K.Nishida, H.Sasaki, et. al.
  • Journal Title: Pharmaceutical Research 24 Pages: 1891-1896
  • Peer Reviewed
• [Journal Article] Unilateral lung-selective gene transfer following the administration of Naked plasmid DNA onto the pulmonary pleural surface in mice (2007)
  • Author(s): J.Nakamura, S.Fumoto, K.Ariyoshi, H.Sasaki, et. al.
  • Journal Title: Biol.Pharm.Bull. 30 Pages: 729-732
  • NAID: 110006271214
  • Peer Reviewed
• [Journal Article] Influence of disease stage on polyethylenimine-mediated plasmid DNA delivery in murine hepatitis (2006)
  • Author(s): Sasaki H, Yoshida S, Kitahara T, Yoshioka T, Nakagawa H, Nakamura T, Ichikawa N, Nishida K, Nakamura J, Nakashima M
  • Journal Title: Int J Pharm 318 Pages: 139-45
  • Peer Reviewed
• [Journal Article] Partial hepatectomy enhances polyethylenimine-mediated plasmid DNA delivery (2006)
  • Author(s): Tada Y, Kitahara T, Yoshioka T, Nakamura T, Ichikawa N, Nakashima M, Nishida K, Nakamura J, Sasaki H
  • Journal Title: Biol Pharm Bull 29 Pages: 1712-6
  • NAID: 110005664015
  • Peer Reviewed
• [Journal Article] Influence of disease stage on polyethyleniine-mediated plasmid DNA delivery in murine fepatitis (2006)
  • Author(s): H.Sasaki, S.Yoshida, T.Kitahara, T.Yoshioka, H.Nakagawa
  • Journal Title: Inter.J.Pharmaceu 318 Pages: 139-145
• [Journal Article] Partial hepatectomy enhances polyethylenimine-mediated plasmid DNA delivery (2006)
  • Author(s): Y.Tada, T.Kitahara, T.Yoshioka, T.Nakamura, H.Sasaki
  • Journal Title: Biol.Pharm.Bull. 29 Pages: 1712-1716
  • NAID: 110005664015
• [Journal Article] Stomach-selective gene transfer following the administration of naked plasmid DNA onto the gastric serosal surface in mice (2006)
  • Author(s): J.Nakamura, S.Fumoto, K.Shoji, Y.Kodama, H.Sasaki
  • Journal Title: Biol.Pharm.Bull. 29 Pages: 2082-2086
  • NAID: 110004810296
• [Journal Article] Prolonged blood concentrationof prednisolone after intravenous injection of liposomal palmitoyl prednisolone (2006)
  • Author(s): M.Teshima, S.Fumoto, K.Nishida, J.Nakamura, H.Sasaki
  • Journal Title: J.Control.Release 112 Pages: 320-328
• [Journal Article] PEGylated liposomes loading palmitoyl prednisolone for prolonged blood concentration of prednisolone (2006)
  • Author(s): M.Teshima, S.Kawakami, S.Fumoto, K.Nishida, H.Sasaki
  • Journal Title: Biol.Pharm.Bull. 29 Pages: 1436-1440
  • NAID: 110005602317
• [Journal Article] In vitro activity of lauric acid or myristylamine in combination with six antimicrobial agents against MRSA (2006)
  • Author(s): T.Kitahara, Y.Aoyama, Y.Hirakata, S.Kanmihira, H.Sasaki
  • Journal Title: Int.J.Antimicrob.Agents 27 Pages: 51-57
• [Presentation] Influence of murine hepatitis induced by D-(+)-galactos amine hydrochloride and lipopolysaccharide on gene explession of polyethyleneimine/pDNA polyplex (2008)
  • Author(s): Sasaki, H, et al.
  • Organizer: 2008 AAPS Annual Meeting and Exposition.
  • Place of Presentation: Atlanta, USA
  • Year and Date: 2008-11-19
• [Presentation] Effect of disease stage on gene expression by polyplex and lipoplex in murine hepatitis induced by carbon tetrachloride (2008)
  • Author(s): Teshima, M., Yoshida, S., Fumoto, S., Nishida, K., Nakamura, J., Nakashima, M., Hamamoto, T., To, H., Kitahara, T., Sasaki, H.
  • Organizer: 23rd JSSX Annual Meeting
  • Place of Presentation: Kumamoto, Japan
• [Presentation] Influence of murine hepatitis induced by D-(+)-galactosamine hydrochloride and lipopolysaccharide on gene explession of polyethyleneimine/pDNA polyplex (2008)
  • Author(s): Sasaki, H., Kurosaki, T., To, H., Kitahara, T.
  • Organizer: 2008 AAPS Annual Meeting and Exposition
  • Place of Presentation: Atlanta, USA
• [Presentation] Topics of Individualised Medicine (2007)
  • Author(s): H.Sasaki
  • Organizer: World Congress of Pharmacy and Pharmaceutical Sciences 2007
  • Place of Presentation: Beijing,China
  • Year and Date: 2007-09-06
• [Presentation] Ichikawa N : Delivery timing of plasmid DNA with non-viral carrier in murine hepatitis (2006)
  • Author(s): Sasaki H, Yoshida S, Kitahara T, To H
  • Organizer: 21st FAPA congress
  • Place of Presentation: Yokohama, Japan
• [Presentation] 肝再生の遺伝子デリバリーに対する影響-肝切除マウスにおけるpolyethylenimine/pDNA複合体の遺伝子発現 (2006)
  • Author(s): 山内浩子, 夛田勇巳, 一川暢宏, 籐秀人, 佐々木均, 中嶋幹郎, 西田孝洋, 中村純三
  • Organizer: 日本薬学会第126年会
  • Place of Presentation: 仙台
• [Presentation] Polyethylenimineを用いた遺伝子デリバリーへの病態の影響-四塩化炭素誘発肝障害マウスにおける検討 (2006)
  • Author(s): 佐々木均, 山内浩子, 夛田勇巳, 一川暢宏, 籐秀人, 中嶋幹郎, 西田孝洋, 中村純三
  • Organizer: 日本薬学会第126年会
  • Place of Presentation: 仙台
• [Presentation] Partial hepatectomy enhances polyethylenimine-mediated plasmid DNA delivery (2006)
  • Author(s): Sasaki H
  • Organizer: The 66th World Congress of Pharmacy and Pharmaceutical Sciences(FIP 2006)
  • Place of Presentation: Salvador Bahia, Brazil