• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

FUNCTIONAL RESEARCH FOR THYMOSIN BETA 4 IN TOOTH GERM DEVELOPMENT

Research Project

Project/Area Number 18590169
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field General anatomy (including Histology/Embryology)
Research InstitutionKyushu University

Principal Investigator

KOBAYASHI Ieyoshi  Kyushu University, Faculty of Dental Science, Associate Professor (40243951)

Co-Investigator(Kenkyū-buntansha) SAKAI Hidetaka  Kyushu University, Faculty of Dental Science, Professor (80136499)
KIYOSHIMA Tamotsu  Kyushu University, Faculty of Dental Science, Assistant Professor (20264054)
Project Period (FY) 2006 – 2007
Project Status Completed (Fiscal Year 2007)
Budget Amount *help
¥3,920,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥420,000)
Fiscal Year 2007: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2006: ¥2,100,000 (Direct Cost: ¥2,100,000)
Keywordsembyologir / morphology / tooth germ / knockdown method / thvmosin beta 4 / nucleolin / anti-sense nucleic acid method / Runx2 / Thymosin beta 4
Research Abstract

Odontogenesis is a complex developmental process that is mediated by a series of epithelial-mesenchymal interactions. We previously detected several intensely expressed genes, e.g. phosphoglycerate kinase 1(Pgk 1), Nucleolin, thymosin beta 4 (Tβ4) and so on at the initial stages of tooth development. Tβ4 was found to be differentially expressed in embryonic day 12 (E12) mouse mandible. Tβ4 was expressed in the dental epithelium at E10.5-18. At E18. In this research project, the function of Tβ4 in developing tooth germ was checked by using Tβ4 antisense phosphorothioated oligonucleotide (AS-S-ODN) in cultured E11 mouse mandible and E 15 tooth germ. Complete suspension of tooth germ formation was observed after 8 days culture of E11 mandible. Inhibition of differentiation of mesenchymal cells to odontoblasts cells was observed after 8 days culture of E15 tooth germ. Deposition of enamel and dentin matrix was also inhibited in the tooth germs cultured with AS-S-ODN. Semi quantitative RT-P … More CR was performed to confirm the inhibition of expression of Tβ4 mRNA, and also to check the effect of Tβ4 AS-ODN on the expression of matrix metalloproteinase (MMP) -2/-9, dentin sialophosphoprotein, dentin marix protein 1, amelogenin and enamelin genes in odontogenic cell culture. Inhibition of Tβ4, MMP-2/-9 and odontogenic factors were detected in Tβ4 AS-S-ODN treated cells. This inhibition assay demonstrates that Tβ4 has important role in morphogenesis of tooth germ and in dentin matrix formation. This result represents that Tβ4 may play role in odontogenesis by controlling expressions of MMPs and odontogenic factors.
In an inhibition assay for Nucleolin, Nucleolin AS S-ODN treatment resulted in an arrest of tooth germ growth at the cap-like stage in cultured E15 mandible. This inhibition assay indicated that Nucleolin took part in the tooth germ initiation and morphogenesis during early stage of tooth germ development. The strong expression of Pgkl mRNA was also seen in theodontogenic epithelial cells and surroundingmesenchymal cells of the tooth germ at E10.5-18.0. Pgkl protein formed 84-kDa protein complex in the embryonic organs. These results suggested this complex to be formed with glyceraldehyde-3-phosphate dehydrogenase(GAPDH), and Pgklplays some functional roles in the development of tooth germ by forming protein complex with GAPDH. We also reported Nucleolin and Pgkl functional roles in the development of tooth in journals (Journal of Biological Chemistry & Histology and Histopathology). Less

Report

(3 results)
  • 2007 Annual Research Report   Final Research Report Summary
  • 2006 Annual Research Report
  • Research Products

    (26 results)

All 2008 2007 2006 2004 Other

All Journal Article (10 results) (of which Peer Reviewed: 6 results) Presentation (14 results) Remarks (2 results)

  • [Journal Article] Glycolytic enzyme Pgk1 is strongly expressed in the developing tooth germ of the mouse lower first molar2008

    • Author(s)
      Jun-ya Honda, et. al.
    • Journal Title

      Histology and Histopathology 23

      Pages: 423-432

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
    • Peer Reviewed
  • [Journal Article] Glycolytic enzyme Pgk 1 is strongly expressed in the developing tooth germ of the mouse lower first molar2008

    • Author(s)
      Jun-ya, Honda, et. al.
    • Journal Title

      Histology and Histopathology 23

      Pages: 423-432

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] Glycolytic enzyme Pgkl is strongly expressed in,the developing tooth germ of the mouse lower first molar2008

    • Author(s)
      Jun-ya Honda, Ieyoshi Kobayashi, Tamotsu Kiyoshima, Hidetaka Sakai, et. al.
    • Journal Title

      Histology and Histopathology 23

      Pages: 423-432

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Age-dependent changes in cell prohjbration and oell death in the periodontal tissue and the submandibular gland in mice:A comparison with other tissues and organs2007

    • Author(s)
      Norio Enoki, et. al.
    • Journal Title

      Journal of Molecular Histology 38

      Pages: 321-332

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
    • Peer Reviewed
  • [Journal Article] Functional Implication of Nucleolin in the Mouse First Molar Development2007

    • Author(s)
      Ming Xie, et. al.
    • Journal Title

      Journal of Biological Chemistry 282

      Pages: 23275-23283

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
    • Peer Reviewed
  • [Journal Article] Age-dependent changes in cell proliferation and cell death in the periodontal tissue and the submandibular gland in mice : A comparison with other tissues and organs2007

    • Author(s)
      Norio, Enoki, et. al.
    • Journal Title

      Journal of Molecular Histology 38

      Pages: 321-332

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] Functional Implication of Nucleolin in the Mouse First Molar Development2007

    • Author(s)
      Ming, Xie, et. al.
    • Journal Title

      Journal of Biological Chemistry 282

      Pages: 23275-23283

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] Functional Implication of Nucleolin in the Mouse First Molar Development2007

    • Author(s)
      Ming Xie, Ieyoshi Kobayashi, Tamotsu Kiyoshima, Hidetaka Sakai, et. al.
    • Journal Title

      Journal of Biological Chemistry 282

      Pages: 23275-23283

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Transcription promoter activity of the human S100A7 gene in oral squamous cell carcinoma cell lines2006

    • Author(s)
      Hideaki Fukuzawa, et. al.
    • Journal Title

      Biochem. Biophys. Acta 1759

      Pages: 171-176

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
    • Peer Reviewed
  • [Journal Article] Transcription promoter activity of the human S100A7 gene in oral squamous cell carcinoma cell lines2006

    • Author(s)
      Hideaki, Fukuzawa, et. al.
    • Journal Title

      Biochem. Biophys 1759

      Pages: 171-176

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] Protogenin a new member of the immunoglobulin superfamily, is implicated in the development of mouse lower first molar2008

    • Author(s)
      Tamotsu Kiyoshima, Keiko Fukiwake, Ieyoshi Kobayahsi, Kengo Nagata, Hidetaka Sakai
    • Organizer
      The Joint Meeting of The 3rd Symposium on"Oral Health Science" and the 3rd Symposium on "Dental and Craniofacial Morphogenesis and Tissue Regeneration"
    • Place of Presentation
      Japan(Fukuoka)
    • Year and Date
      2008-01-26
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] Roles of thymosin beta 4 and nucleolin in the developing tooth germ of the mouse lower first Inolar2008

    • Author(s)
      Ieyoshi Kobayahsi, Tamotsu Kiyoshima, Kengo Nagata, Hidetaka Sakai
    • Organizer
      The Joint Meeting of The 3rd Symposium on"Oral Health Science" and the 3rd Symposium on "Dental and Craniofacial Morphogenesis and Tissue Regeneration"
    • Place of Presentation
      Japan(Fukuoka)
    • Year and Date
      2008-01-26
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] Keiko Fukiwake, Ieyoshi Kobayashi, Kengo Ngata, Hidetaka Sakai, Protogenin, a new member of the imnaunoglobulin superfamily is implicated in the development of mouse lower first molar2008

    • Author(s)
      Tamotsu, Kiyoshima
    • Organizer
      The Joint Meeting of the 3th Symposium on "Oral Health Science" and the 3th Symposium on "Dental and Craniofacial Morphogenesis and Tissue Regeneration"
    • Place of Presentation
      Japan, (Fukuoka)
    • Year and Date
      2008-01-26
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] Tamotsu Kiyoshima, Kengo Ngata, Hidetaka Sakai, Roles of thymosin beta 4 and nucleolin in the developing tooth germ of implicated in the development of mouse lower first molar the mouse lower first molar2008

    • Author(s)
      Ieyoshi, Kobayashi
    • Organizer
      The Joint Meeting of the 3rd Symposium on "Oral Health Science" and the 3th Symposium on "Dental and Craniofacial Morphogenesis and Tissue Regeneration"
    • Place of Presentation
      Japan, (Fukuoka)
    • Year and Date
      2008-01-26
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] 歯胚発に関わる新規遺伝子Clone15の機能解析2007

    • Author(s)
      吹譯 景子, 清島 保, 小林 家吉, 榎 規雄, 寺田 善博, 坂井 英隆
    • Organizer
      第49回歯科基礎医学会学術大会&総会
    • Place of Presentation
      札幌
    • Year and Date
      2007-08-31
    • Related Report
      2007 Annual Research Report
  • [Presentation] 歯胚発生過程におけるNucleolinおよびThymosin beta 4 の役割について2007

    • Author(s)
      小林 家吉, 清島 保, 吹譯 景子, 榎 規雄, 永田 健吾, 坂井 英隆
    • Organizer
      第49回歯科基礎医学会学術大会&総会
    • Place of Presentation
      札幌
    • Year and Date
      2007-08-30
    • Related Report
      2007 Annual Research Report
  • [Presentation] Transcriptional Regulation of S100A7 in Oral Squamous Cell Carchloma2006

    • Author(s)
      Tamotsu Kiyoshima
    • Organizer
      The International Association for Dental Research(84th
    • Place of Presentation
      Australia(Brisbane)
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] Roles of Thymosin beta 4 in Developing tooth Germ2006

    • Author(s)
      Ieyoshi Kobayashi
    • Organizer
      The 16th International Microscopy Congress
    • Place of Presentation
      Japan(Sapporo)
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] Expression and Possible Functional Role of Nucleolin in The Mouse First Molar Development2006

    • Author(s)
      Ming Xie
    • Organizer
      The 16th International Microscopy Congress
    • Place of Presentation
      Japan(Sapporo)
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] Transcriptional Regulation of S 100A7 in Oral Squamous Cell Carcinoma2006

    • Author(s)
      Tamotsu, Kiyoshima
    • Organizer
      The International Association for Dental Research(84th)
    • Place of Presentation
      Australia, (Brisbane)
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] Possible Function of Thymosin beta 4 in Developing Tooth Germ2006

    • Author(s)
      Keiko, Takahashi
    • Organizer
      The International Association for Dental Research(84th)
    • Place of Presentation
      Australia, (Brisbane)
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] Roles of ymosin beta 4 in Developing Too Germ2006

    • Author(s)
      Ieyoshi, Kobayashi
    • Organizer
      The 16th International Microscopy Congress
    • Place of Presentation
      Japan, (Sapporo)
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] Expression and Possible Functional Role of Nucleolin in The Mouse First Molar Development2006

    • Author(s)
      Ming, Xie
    • Organizer
      The 161 International Microscopy Congress
    • Place of Presentation
      Japan, (Sapporo)
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] Possible Function of Thymosin beta 4 in tooth Development2004

    • Author(s)
      Keiko Takahashi
    • Organizer
      The International Association for Dental Research(84th
    • Place of Presentation
      Australia(Brisbane)
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Remarks] 「研究成果報告書概要(和文)」より

    • URL

      http://hyoka.ofc.kyushu-u.ac.jp/search/details/KOO1037/research.html

    • Related Report
      2007 Final Research Report Summary
  • [Remarks]

    • URL

      http://hyoka.ofc.kyushu-u.ac.jp/search/detais/k001037/research.html

    • Related Report
      2007 Annual Research Report

URL: 

Published: 2006-04-01   Modified: 2016-04-21  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi