| Project/Area Number |
18590175
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
General anatomy (including Histology/Embryology)
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| Research Institution | Kyorin University |
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Principal Investigator |
KANAI Masami Kyorin University, Faculty of Medicine, Assistant professor (70321883)
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| Co-Investigator(Kenkyū-buntansha) |
YONEKAWA Hiromichi Tokyo Metropolitan Institute of Medical Science, 東京都臨床医学研究所, Vice director (30142110)
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| Project Period (FY) |
2006 – 2007
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| Project Status |
Completed (Fiscal Year 2007)
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| Budget Amount *help |
¥3,880,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥480,000)
Fiscal Year 2007: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Fiscal Year 2006: ¥1,800,000 (Direct Cost: ¥1,800,000)
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| Keywords | endoderm / mouse / development and differentiation / gut formation / germ cells / 始原生殖細胞 / Sox17 |
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| Research Abstract |
The Sox (Sryrelated HMG box) gene family was first identified through its homology to the high mobility group (HMG) box of the sex detemininating gene of SRY In this project, we have studied that the role of Sox17 on the mouse endoderm, and especially of interaction between primodial germ cells (PGCs) and primary gut. The migration of PGCs are observed after the gastrulaion stage of the embryo and they have moved from allantois to the genital ridge through hind gut. PGCs are initially highly motile but restricted to the hindgut region, from where they then migrate out and accumulate in the genital ridge. However, the mutant of Sox17 shows the accumulation of PGCs around the allantois. The number of PGCs are not affected by Sox17 null mutant. Therefore, we have generated the chimera between CAG-EGFP blastocyst and Sox 17-/- ES cells, and observed the ineraction of PGCs and hindgut cells.
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