Morphofimctional analysis ofGalanin-lice Peptide on regulation offeeding and enegy metabolism

• Project/Area Number: 18590176
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: General anatomy (including Histology/Embryology)
• Research Institution: Showa University
• Principal Investigator: GUAN Jian-Lian Showa University, Dept. of Anatomy, Sch. of Med., Assistant Professor (20276538)
• Co-Investigator(Kenkyū-buntansha): SHIODA Seiji Showa University, Department of Analomy School of Medicine, Professor (80102375) ; ARATA Satoru Showa Univ., Center for Biotechnology, Associate Professor (20159502) ; OSAKA Toshimasa National Institute of Health and Nutrition, 栄養所要量研究部, Senior Researcher (30152101)
• Project Period (FY): 2006 – 2007
• Project Status: Completed (Fiscal Year 2007)
• Budget Amount: ¥3,820,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥420,000); Fiscal Year 2007: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000); Fiscal Year 2006: ¥2,000,000 (Direct Cost: ¥2,000,000)
• Keywords: Galanin-like peptide / afferent neuron / energy metabolism / Transgenic mice / neuron network

Research Abstract

The hypothalamus plays a critical role in regulation of feeding behavior, energy metabolism and reproduction. A novel peptide discovered in porcine hypothalamus has 60 amino acid peptide and a non-amidated C-terminus. Since a portion of this peptide is homology with galanin, it is named galanin-like peptide (GALP). GALP is mainly produced in the hypothalamic arcuate nucleus. GALP acts in the central nervous system, where it is involved in the regulation of feeding behavior. GALP-producing neurons were shown to make neuron networks with several feeding related peptide-producing neurons in the hypothalamus. Since GALP controls food intake and energy balance, it is possible that GALP is an important factor in development of obesity. Furthermore, we have succeeded to make transgenic (Tg) mice that express enhanced green fluorescence (EGFP) in GALP-containing neurons and also to make Tg mice that express EGFP in afferent GALP neurons in brain. As a result, we have first shown that GALP-containing neurons make synaptic input from the pyriform cortex and amygdala. Therefore, GALP may involve in feeding behavior, energy metabolism and reproduction, further studies on the morphology and function of GALP should continue to demonstrate further the understanding in brain function.

Research Products

• [Journal Article] Visualization of ghrelin-producing neurons in the hypothalamic arcuate nucleus using ghrelin-EGFP transgenic mice (2008)
  • Author(s): Kageyama H.
  • Journal Title: Regul Pept. 145 Pages: 116-121
  • Description: 「研究成果報告書概要(和文)」より
  • Peer Reviewed
• [Journal Article] Synaptic relationships between proopiomelanocortin-and ghrelin-containing neurons in the rat arcuate nucleus (2008)
  • Author(s): Guan JL.
  • Journal Title: Regul Pept. 145 Pages: 128-132
  • Description: 「研究成果報告書概要(和文)」より
  • Peer Reviewed
• [Journal Article] Synaptic interaction between ghrelin-and ghrelin-containing neurons in the rat hypothalamus (2008)
  • Author(s): Hori Y.
  • Journal Title: Regul Pept. 145 Pages: 122-127
  • Description: 「研究成果報告書概要(和文)」より
  • Peer Reviewed
• [Journal Article] Visualization of ghrelin-producing neurons in the hypothalamic arcuate nucleus using ghrelin-EGFP transgenic mice (2008)
  • Author(s): Kageyama H.
  • Journal Title: Regul Pept. 145・1-3 Pages: 116-121
  • Peer Reviewed
• [Journal Article] Synaptic relationships between proopiomelanocortin- and ghrelin-containing neurons in the rat arcuate nucleus (2008)
  • Author(s): Guan JL.
  • Journal Title: Regul Pept. 145・1-3 Pages: 128-132
  • Peer Reviewed
• [Journal Article] Synaptic interaction between ghrelin- and ghrelin-containing neurons in the rathypothalamus (2008)
  • Author(s): Hori Y.
  • Journal Title: Regul Pept. 145・1-3 Pages: 122-127
  • Peer Reviewed
• [Journal Article] Morphological interaction between galanin-like peptide- and dopamine-containing neurons in the rat arcuate nucleus (2008)
  • Author(s): Kageyama H.
  • Journal Title: Regul Pept. 145・1-3 Pages: 165-168
  • Peer Reviewed
• [Journal Article] Neuronal interactions between neuropeptide W- and orexin- or melanin-concentrating hormone-containing neurons in the rat hypothalamus (2008)
  • Author(s): Takenoya F.
  • Journal Title: Regul Pept. 145・1-3 Pages: 159-164
  • Peer Reviewed
• [Journal Article] Peptidomic identification and biological validation of neuroendocrine regulatory peptide-1 and -2 (2007)
  • Author(s): Yamaguchi H.
  • Journal Title: J Biol Chem. 282・36 Pages: 323-331
  • Peer Reviewed
• [Journal Article] synaptic relationships proopiomelanocortin-and ghrelin-containing neurons in the rat a arcuate nucleus (2007)
  • Author(s): Guan JL.
  • Journal Title: Rregul Pept (in press)
• [Journal Article] ガラニン様ペプチド(GALP)の接触調節及びエネルギー代謝調節機構 (2006)
  • Author(s): 影山晴秋
  • Journal Title: 内分泌代謝・糖尿病科 22・4 Pages: 394-400
• [Journal Article] Neuronal interactions between galanin-like peptide(GALP)-and luteinizing hormone-releasing hormone(LHRH)-containing neurons. (2006)
  • Author(s): Takenoya F.
  • Journal Title: Peptides 27 Pages: 2885-2893
• [Journal Article] Galanin-like peptide promotes feeding behavior via orexin neurons in the rat hypothalamus. (2006)
  • Author(s): Kageyama H.
  • Journal Title: J Neuroendocrinol 18 Pages: 33-41
• [Journal Article] Peripheral ghrelin transmits starvation signals through the noradrenergic pathway from the hindbrain to the hypothalamus. (2006)
  • Author(s): Date Y.
  • Journal Title: Cell Metab 4 Pages: 323-331
• [Journal Article] Pituitary adenylate cyclase-activating.polypeptide(PACAP)decreases neuronal cell death after focal ischemia associated with interlerukin-6(IK-6). (2006)
  • Author(s): Ohtaki H.
  • Journal Title: Proc Natl A cad Sci 103 Pages: 7486-7493
• [Journal Article] Pleiotropic Functions of PACAP in the CNS : Neuroprotection and Neurodevelopment. (2006)
  • Author(s): Shioda S.
  • Journal Title: Ann NY Acad Sci 1070 Pages: 550-560
• [Journal Article] Central administration of melanin-concentrating hormone(MCH) suppresses food intake,but not locomotion activity, ina the goldfish, Carassius auratus (2006)
  • Author(s): Matsuda K.
  • Journal Title: Neurosci Lett 399 Pages: 259-263
• [Journal Article] Direct involvement of orexinergic system in the activation of mesolimbic dopamine pathway and its related behavior induced by morphine. (2006)
  • Author(s): Narita M.
  • Journal Title: J Neurosci 26 Pages: 398-405
• [Journal Article] Neuropeptide W is present in antral G cells of rat, mouse, and human stomach. (2006)
  • Author(s): Mondal MS.
  • Journal Title: J Endocrinol 188 Pages: 49-57
• [Journal Article] 2006 Galanin-like peptide promotes feeding behaviour via activation of orexinergic neurones in the rat lateral hypothalamus
  • Author(s): Kageyama, H, Kita, T, Toshinai, K, Guan, JL, Date, Y, Takenoya, F, Kato, S, Matsumoto, H, Ohtaki, T, Nakazato M, Shioda, S
  • Journal Title: J Neuroendocrinol 18 Pages: 33-41
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] 2006 Central administration of melanin-concentrating hormone(MCH) suppresses food intake, but not locomotor activity, in the goldfish, Carassius auratus
  • Author(s): Matsuda, K, Shimakura, S, Maruyama, K, Miura, T, Uchiyama, M, Kawauchi, H, Shioda, S, Takahashi, A
  • Journal Title: Neurosci Lett 399 Pages: 259-63
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] 2006 Neural interaction between galanin-like peptide(GALP)- and luteinizing hormone-releasing hormone(LHRH)-containing neurons
  • Author(s): Takenoya, F, Guan, JL, Kato, M, Sakuma, Y, Kintaka, Y, Kitamura, Y, Kitamura, S, Okuda, H, Takeuchi, M, Kageyama, H, Shioda, S
  • Journal Title: Peptides 27 Pages: 2885-93
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] 2006 Peripheral ghrelin transmits orexigenic signals through the noradrenergic pathway from the hindbrain to the hypothalamus
  • Author(s): Date, Y, Shimbara, T, Koda, S, Toshinai, K, Ida, T, Murakami, N, Miyazato, M, Kokame, K, Ishizuka, Y, Ishida, Y, Kageyama, H, Shioda, S, Kangawa, K, Nakazato, M
  • Journal Title: Cell Metab 4 Pages: 323-31
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] 2008 Visualization of ghrelin-producing neurons in the hypothalamic arcuate nucleus using ghrelin-EGFP transgenic mice
  • Author(s): Kageyama, H, Kitamura, Y, Hosono, T, Kintaka, Y, Seki, M, Takenoya, F, Hori, Y, Nonaka, N, Arata, S, Shioda, S
  • Journal Title: Regul Pept 145 Pages: 116-21
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] 2008 Synaptic interaction between ghrelin- and ghrelin-containing neurons in the rat hypothalamus
  • Author(s): Hori, Y, Kageyama, H, Guan, JL, Kohno, D, Yada, T, Takenoya, F, Nonaka, N, Kangawa, K, Shioda, S, Yoshida, T
  • Journal Title: Regul Pept 145 Pages: 122-7
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] 2008 Synaptic relationships between proopiomelanocortin- and ghrelin-containing neurons in the rat arcuate nucleus
  • Author(s): Guan, JL, Okuda, H, Takenoya, F, Kintaka, Y, Yagi, M, Wang, L, Seki, M, Hori, Y, Kageyama, H, Shioda, S
  • Journal Title: Regul Pept 145 Pages: 128-32
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] 2008 Neuronal interactions between neuropeptide W- and orexin- or melanin-concentrating hormone-containing neurons in the rat hypothalamus
  • Author(s): Takenoya, F, Kitamura, S, Kageyama, H, Nonaka, N, Seki, M, Itabashi, K, Date, Y, Nakazato, M, Shioda, S
  • Journal Title: Regul Pept 145 Pages: 159-64
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] 2008 Morphological interaction between galanin-like peptide- and dopamine-containing neurons in the rat arcuate nucleus
  • Author(s): Kageyama, H, Takenoya, F, Hori, Y, Yoshida, T, Shioda, S
  • Journal Title: Regul Pept 145 Pages: 165-8
  • Description: 「研究成果報告書概要(欧文)」より
• [Presentation] 摂食調節ニューロンの求心性神経回路網の形態機能解析 (2008)
  • Author(s): 影山 晴秋
  • Organizer: 第113回日本解剖学会総会、全国学術集会
  • Place of Presentation: 大分大学医学部
  • Description: 「研究成果報告書概要(和文)」より
• [Presentation] 摂食調節ニューロンの求心性神経回路網の形態機能解析 (2008)
  • Author(s): 影山 晴秋
  • Organizer: 第113回日本解剖学会総会・全国学術集会
  • Place of Presentation: 大分大学医学部
• [Presentation] Cre-LoxPシステムによるCaMKH発現ニューロンのニューロンネットワークの解析 (2008)
  • Author(s): 影山 晴秋
  • Organizer: 第113回日本解剖学会総会・全国学術集会
  • Place of Presentation: 大分大学医学部