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Molecular and Cell Biological Studies on Functional Proteins of Skeletal Muscle

Research Project

Project/Area Number 18590180
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field General anatomy (including Histology/Embryology)
Research InstitutionGunma University

Principal Investigator

YORIFUJI Hiroshi  Gunma University, GRADUATE SCHOOL OF MEDICINE, PROFESSOR (00158544)

Co-Investigator(Kenkyū-buntansha) SATO Mahito  GUNMA UNIVERSITY, GRADUATE SCHOOL OF MEDICINE, ASSISTANT PROFESSOR (60375532)
TAJIKA Yuki  GUNMA UNIVERSITY, GRADUATE SCHOOL OF MEDICINE, ASSISTANT PROFESSOR (90400738)
Project Period (FY) 2006 – 2007
Project Status Completed (Fiscal Year 2007)
Budget Amount *help
¥3,960,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥360,000)
Fiscal Year 2007: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2006: ¥2,400,000 (Direct Cost: ¥2,400,000)
Keywordsskeletal muscle / VAMP / regeneration / ontogeny / cytoskeletal protein / 筋再生
Research Abstract

Functional proteins of skeletal muscles were studied firm two aspects. One is the turnover of membrane proteins and we concentrated on the control proteins of membrane fusion, especially VAMP (Vesicle Associate Membrane Proteins) family proteins. In mature skeletal muscle of the rat, strong expression of VAMP2 was found in satellite cells. Regenerating muscles which had been injected cardiotoxin showed bright VAMP2 fluorescence at 3 days after injection. During ontogenesis, it appeared in myoblasts of the somites at embyonic day 12 (E12), and remained positive in multi-nucleated myotube at E16. VAMP2 may be involved in myogenesis. In mature skeletal muscles, VAMP1 was found at the nerve terminals of neuromuscular junctions and VAMP3 in endothelial cells of endo- and peri-mysium. The other is an analysis of I vivo function of a cytoskeletal protein of the skeletal muscle. Knockout mice were tried to be generated Heterozygote mice have been obtained and are now under mating to get homozygote animals.

Report

(3 results)
  • 2007 Annual Research Report   Final Research Report Summary
  • 2006 Annual Research Report
  • Research Products

    (10 results)

All 2008 2007

All Journal Article (4 results) (of which Peer Reviewed: 2 results) Presentation (6 results)

  • [Journal Article] VAMP2 is expressed in muscle satellite cells and up-regulated during muscle regeneration.2007

    • Author(s)
      Tajika Y, Sato M, Murakami T, Takata K & Yorifuji H.
    • Journal Title

      Cell and Tissue Research 328

      Pages: 573-581

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
    • Peer Reviewed
  • [Journal Article] VAMP2 is expressed in muscle satellite cell and up-regulated during imauscle regeneration2007

    • Author(s)
      Tajika Y, Sato M, Murakami T, Takata K & Yorifuji H
    • Journal Title

      Cell and Tissue Research 328(3)

      Pages: 573-581

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] VAMP2 is expressed in muscle satellite cells and up-regulated during muscle regeneration2007

    • Author(s)
      Tajika Y, Sato M, Murakami T, Takata K & Yorifuji H.
    • Journal Title

      Cell and Tissue Research 328

      Pages: 573-581

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Journal Article] VAMP2 is expressed in muscle satellite cells and up-regulated during muscle regeneration.2007

    • Author(s)
      Y.Tajika, M.Sato, T.Murakami, K.Takata, H.Yorifuji
    • Journal Title

      Cell and Tissue Research (in press web上では既に掲載済)

    • Related Report
      2006 Annual Research Report
  • [Presentation] マウス骨格筋組織におけるVAMP5の分布2008

    • Author(s)
      多鹿友喜、佐藤真人、村上 徹、依藤 宏
    • Organizer
      第113回日本解剖学会総会・全国学術集会
    • Place of Presentation
      大分大学医学部
    • Year and Date
      2008-03-27
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Annual Research Report 2007 Final Research Report Summary
  • [Presentation] Expression of VAMP5 in mouse skeletal muscle2008

    • Author(s)
      Yuki Tajika, Mahito Sato, Tohru Murakami, Hiroshi Yorifuji
    • Organizer
      113th Annual Meeting of Japanese Association of Anatomists
    • Place of Presentation
      Ohita
    • Year and Date
      2008-03-27
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] 体節におけるVAMP2の発現2007

    • Author(s)
      多鹿友喜、佐藤真人、村上 徹、依藤 宏
    • Organizer
      第48回日本組織細胞化学会
    • Place of Presentation
      甲府市総合市民会館
    • Year and Date
      2007-09-28
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Annual Research Report 2007 Final Research Report Summary
  • [Presentation] Expression of VAMP2 in somite2007

    • Author(s)
      Yuki Tajika, Mahito Sato, Tohru Murakami, Hiroshi Yorifuji
    • Organizer
      48th Annual Meeting of Japan Society if Histochemistry and Cytochemistry
    • Place of Presentation
      Kofu
    • Year and Date
      2007-09-28
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] 筋衛星細胞および再生筋におけるVesicle-Associated Membrane Protein-2 (VAMP2)の発現と分布2007

    • Author(s)
      多鹿友喜、佐藤真人、村上 徹、依藤 宏
    • Organizer
      第112回日本解剖学会総会・全国学術集会
    • Place of Presentation
      大阪国際会議場
    • Year and Date
      2007-03-29
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] Expression of Vesicle-Associated Membrane Protein-2 (VAMP2) in muscle satellite cells and regenerating myotubes2007

    • Author(s)
      Yuki Tajika, Mahito Sato, Tohru Murakami, Hiroshi Yorifuji
    • Organizer
      112th Annual Meeting of Japanese Association of Anatomists
    • Place of Presentation
      Osaka
    • Year and Date
      2007-03-29
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary

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Published: 2006-04-01   Modified: 2016-04-21  

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