Morphofunctional studies on the Protein4.1B deficient mouse body
| Project/Area Number |
18590181
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
General anatomy (including Histology/Embryology)
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| Research Institution | University of Yamanashi |
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Principal Investigator |
TERADA Nobuo University of Yamanashi, 大学院・医学工学総合研究部, 准教授 (60293461)
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| Project Period (FY) |
2006 – 2008
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| Project Status |
Completed (Fiscal Year 2008)
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| Budget Amount *help |
¥3,940,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥540,000)
Fiscal Year 2008: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2007: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2006: ¥1,600,000 (Direct Cost: ¥1,600,000)
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| Keywords | 分子形態学 / プロテイン4.1 / 膜骨格 / 免疫組織化学 / 凍結技法 / 細胞接着 / 生体内凍結 / 共焦点ラマン顕微法 / 細胞膜 / 遺伝子欠損マウス / 電子顕微鏡 / 接着 / ラマン顕微鏡 / レプリカ技法 / 膜骨格蛋白 / 膜内蛋白 / イオン共輸送体 / Protein4.1 |
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| Research Abstract |
赤血球膜裏側の血管ずり応力に抗する「膜骨格」構成蛋白プロテイン4.1B の臓器組織での局在と病態変化を研究した。4.1B は腎臓でナトリウム-重炭酸イオン共輸送体と、さらに副腎髄質ホルモン分泌細胞で膜貫通細胞接着蛋白TSLC1 と結合していた。また急速凍結による電子顕微鏡3次元観察により膀胱上皮傘細胞のダイナミン蛋白による伸縮機構を明らかにした。さらに生体内凍結技法により血管内ヘモグロビン分子構造変化を瞬時に止めて酸素飽和度の分布をラマン顕微鏡で得た。
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Report
(4 results)
Research Products
(76 results)
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[Journal Article] Dispensable role of protein 4.1B/DAL-1 in rodent adrenal medulla regarding generation of pheochromocytoma and plasmalemmal localization of TSLC12009
Author(s)
Ohno N, Terada N, Komada M, Saitoh S, Costantini F, Pace V, Germann PG, Weber K, Yamakawa H, Ohara O, Ohno S.
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Journal Title
Related Report
Peer Reviewed
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[Journal Article] Interaction of membrane skeletal protein, protein 4.1B and p55, and sodium bicarbonate cotransporter1 in mouse renal S1-S2 proximal tubules.2007
Author(s)
Terada N, Ohno N, Saitoh S, Seki G, Komada M, Suzuki T, Yamakawa H, Soleimani M, Ohno S.
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Journal Title
J Histochem Cytochem 55(12)
Pages: 1199-206
Related Report
Peer Reviewed
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[Journal Article] Conservation and alteration of chromosome territory arrangements in thyroid carcinoma cell nuclei2007
Author(s)
Murata S, Nakazawa T, Ohno N, Terada N, Iwashina M, Mochizuki K, Kondo T, Nakamura N, Yamane T, Iwasa S, Ohno S, Katoh R.
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Journal Title
Thyroid 17(6)
Pages: 489-96
Related Report
Peer Reviewed
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[Journal Article] Interaction of membrane skeletal protein, protein 4.1B and p55, and sodium bicarbonate cotransporterl in mouse renal S1-S2 proximal tubules.2007
Author(s)
Terada N, Ohno N, Saitoh S, Seki G, Komada M, Suzuki T, Yamakawa H, Soleimani M, Ohno S.
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Journal Title
J Histochem Cytochem 55
Pages: 1199-1206
Related Report
Peer Reviewed
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