| Project/Area Number |
18590188
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
General anatomy (including Histology/Embryology)
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| Research Institution | Kyushu University |
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Principal Investigator |
HIROSE Eiji Kyushu University, Department of Developmental Molecular Anatomy, Graduate School of Medical Sciences, Assistant Professor (40380620)
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| Co-Investigator(Kenkyū-buntansha) |
INAI Tetsuichiro KYUSHU UNIVERSITY, Department of Developmental Molecular Anatomy, Graduate School of Medical Sciences, Associate Professor (00264044)
SHIBATA Yosaburo KYUSHU UNIVERSITY, Department of Developmental Molecular Anatomy, Graduate School of Medical Sciences, Professor (90037482)
NISHITANI Hideo University of Hyogo, Laboratory of Biological Signaling, Graduate School of Life Science, Professor (40253455)
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| Project Period (FY) |
2006 – 2007
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| Project Status |
Completed (Fiscal Year 2007)
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| Budget Amount *help |
¥3,800,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥300,000)
Fiscal Year 2007: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2006: ¥2,500,000 (Direct Cost: ¥2,500,000)
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| Keywords | Anatomy / Cell Histology / Ultrastructure of the cell / Genetics / G protein / Xenonus laevis / Xenopus leavis |
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| Research Abstract |
For the first step of investigating the functions of Rag gene family molecules in nuclear division process, we isolated the homologues of Rag gene from African clawed frog, Xenopus laevis (XRag1 and XRag2). A multiple alignment and phylogenetic tree indicated that all eukaryotes carry two closely related genes. Together with binding of these two gene products in human and yeast previously reported, XRag1 and XRag2 could form the minimal functional complex in vivo. However, a level of XRag1 and XRag2 transcripts were not linked revealed by RT-PCR. XRag2 expression showed rapid decrease after Mid Blastula Transition (MBT) in contrast to the almost constant level of XRag2. Then, we confirmed the spatial expression patterns of two genes by in situ hybridization. In early stages before MBT, both XRag1 and XRag2 are expressed in animal hemisphere but XRag2 is decreased after stage 8 and no longer detectable in stage 12. Simultaneous expression of XRag1 and XRag2 was detected again after stage 25, tail bud stage in neuronal tissues (eye and ear primordium). In summary, (1) XRag1 and XRag2 shared incompatible genetic functions. (2) During stage 10-25, only XRag1 is expressed. This suggests XRag1 homodimer (or homooligomer) is the functional unit in these stages. (3) Thus, expression ratio of XRag1 and XRag2 may regulate the switching of the cell division mode at MBT. (4) In later stages, these genes are specifically expressed in neuronal tissues, suggesting new XRag1 and XRag2 roles in the development of peripheral nervous system.
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