| Project/Area Number |
18590190
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
General anatomy (including Histology/Embryology)
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| Research Institution | UNIVERSITY OF MIYAZAKI |
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Principal Investigator |
SUGANUMA Tatsuo UNIVERSITY OF MIYAZAKI, 理事・副学長 (60115350)
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| Co-Investigator(Kenkyū-buntansha) |
SAWGUCHI Akira UNIVERSITY OF MIYAZAKI, MEDICINE, ASSOCIATE PROFESSOR (30336292)
IDE Soyuki UNIVERSITY OF MIYAZAKI, MEDICINE, INSTRUCTOR (20244212)
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| Project Period (FY) |
2006 – 2007
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| Project Status |
Completed (Fiscal Year 2007)
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| Budget Amount *help |
¥3,850,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥450,000)
Fiscal Year 2007: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2006: ¥1,900,000 (Direct Cost: ¥1,900,000)
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| Keywords | HIGH-PRESSURE FREEZING / GASTRIC MUCOSA / ELECTRON MICROSCOPE / GASTRIC-PIT PARIETAL CELL / ISOLATED GASTRIC MUCOSA / STIMULATION OF ACID SECRETION / HISTOCHEMISTRY / AUTOPHAGOSOME / 粘液 / 壁細胞 / ラット |
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| Research Abstract |
To elucidate a functional transformation of gastric parietal cells, we have newly developed an isolated rat gastric mucosa model whose parietal cells exhibited a reverting process from the active to the resting state of acid secretion. Immunohistochemistry of proton pump demonstrated a progressive translocation of proton pump from the apical to the cytoplasmic region. At the ultrastructural level, the apical membranes were subsequently internalized into the cytoplasm forming unique penta-laminar membranes. They then formed a number of multilamellar autophagosomes that were intensely labeled for proton pump. The parietal cells exhibited well-developed Golgi apparatus and lysosomal compartments involving the multilamellar membranes. The present findings provide new insights into the functional transformation in gastric parietal cells reverting to their resting conformation.
It is clinicopathologically important to elucidate the cell kinetics for the maintenance of normal gastric epithelium. In rat gastric mucosa isolated after stimulation, a number of cells were exfoliated into the gastric lumen of the pit region. It was undertaken to clarify the origin of exfoliated cells and their histochemical profiles by taking the advantages of cryotechniques. Most of the exfoliated cells were identified as pit-parietal cells labeled with both peanut-lectin and anti-proton pump antibody. Quantitative analysis verified a time-dependent increase in the number of exfoliated cells in the gastric mucosa isolated after stimulation. Most of the exfoliated cells were negative to the apoptotic markers such as TUNEL staining and caspase-3. Ultrastructurally, autophagusome-like structures consisting of proton pump positive membranes were frequently seen in the exfoliated pit-parietal cells. In addition, the pit-parietal cell exfoliation was accompanied by sealing of their basal portion with the cytoplasmic processes of adjacent surface mucous cells.
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