| Project/Area Number |
18590193
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
General anatomy (including Histology/Embryology)
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| Research Institution | Kitasato University |
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Principal Investigator |
YAMASHINA Shohei Kitasato University, School of Medicine, Emeritus Professor (90013987)
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| Co-Investigator(Kenkyū-buntansha) |
KADOYA Yuichi Kitasato Univ., School of Medicine, Assistant Professor (10185887)
TAMAKI Hideaki Kitasato Univ., School of Medicine, Assistant Professor (30155246)
HAYASHI Keiko Kitasato Univ., School of Medicine, Research Associate (30240211)
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| Project Period (FY) |
2006 – 2007
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| Project Status |
Completed (Fiscal Year 2007)
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| Budget Amount *help |
¥3,650,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥150,000)
Fiscal Year 2007: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000)
Fiscal Year 2006: ¥3,000,000 (Direct Cost: ¥3,000,000)
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| Keywords | Embryology / Histogenesis / Cellular Differentiation / 3-D reconstruction / Secretory Organ / Tissue Stem Cell / Intercalated Duct Cell / Pancreas / 発生学・形態形成学 / 実験形態学 / 組織学 / 細胞微細形態学 / 細泡分化・組織形成 |
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| Research Abstract |
1. Identification of tissue stem cells in the salivary gland In the rat parotid gland after repeated injection by isoproterenol, differentiation potential was studied by means of multiple immunohistochemistry. As a result, followings were concluded. (1) Terminal cells in the intercalated duct differentiate toward acinar cells at 3rd days after IPR treatment. (2) Differentiation was not demonstrated in cells in other portion of intercalated duct by the experimental system.
2. Differntiation potential of intercalated duct cells after the partial pancreatectomy During the course of regeneration after the partial pancreatectomy, 3-D reconstruction was performed from serial section after the treatment of multiple immunohistochemistry of cytokeratin 20 and insulin. As a result, it was found that cells at the terminal portion of intercalated duct differentiated toward acinar cells and cells at the branching portion of intercalated duct differentiated into endocrine cells.
3. Determination of endocrine stem cells in the developing rat pancreas Development of pancreatic islets was investigated by means of 3-D reconstruction treated by multiple immunohistochemistry. Glucagon positive cells were found to appear in the various portion of tubulo-acinar structure during 15 to 17 days in embryonic days, and plural endocrine cells gathered to form a single islet, indicating that the endocrine cells in a islet were not a clone but multiple origin.
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