| Project/Area Number |
18590195
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
General anatomy (including Histology/Embryology)
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| Research Institution | Keio University |
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Principal Investigator |
YAMASHITA Shuji Keio University, School of Medicine, Assistant professor (90050666)
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| Project Period (FY) |
2006 – 2007
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| Project Status |
Completed (Fiscal Year 2007)
|
| Budget Amount *help |
¥3,860,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥360,000)
Fiscal Year 2007: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2006: ¥2,300,000 (Direct Cost: ¥2,300,000)
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| Keywords | DES / malformation / extracellular matrix / angiogenesis / uterus / immunohistochemistry / collagen / MMP |
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| Research Abstract |
1. Matrix metalloproteinase (MMP) expression
The mRNA expression of MMP-2 and MMP-9 was shown to decrease by using RT-PCR in the uteri of 10-day-old mice exposed to diethylstilbestrol neonatally (DES-mice)than those in vehicle-treated control mice. Immunohistochemical study demonstrated that MMP-2 expression significantly decreased in the uteri of DES-mice. In situ zymography using fluorescence-labeled gelatin, enzyme activity of MMP-2 was not detected in the uteri of both experimental groups, although granulocytes showing MMP-9 enzyme activity increased in the DES-mice uteri.
2. Tissue inhibitor of metalloproteinase (TIMP)expression
RT-PCR showed that no significant differences in the mRNA levels of TIMP-1, -2, -3 and -4 between the control and DES-mice.
3. Collagen expression
The mRNA expression of collagen type-I, -III and -IV was shown to be almost the same in uteri of the control and DES-mice by using RT-PCR.
4. Vascular endothelial growth factor (VEGF) expression
Immunohistoichemical study showed that VEGF expression in the uterine stromal cells was much lower level than in those of controls.
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