| Project/Area Number |
18590206
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
General physiology
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| Research Institution | Fukushima Medical University |
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Principal Investigator |
HAZAMA Akihiro Fukushima Medical University, School of Medicine, Professor (60218394)
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| Co-Investigator(Kenkyū-buntansha) |
KATSUDA Shin-ichiro Fukushima Medical University, School of Medicine, Lecturer (80285022)
YAMASAKI Masao Fukushima Medical University, School of Medicine, Associate Professor (10192395)
MIYAKE Masao Fukushima Medical University, school of Medicine, Research Associate (00381385)
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| Project Period (FY) |
2006 – 2007
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| Project Status |
Completed (Fiscal Year 2007)
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| Budget Amount *help |
¥3,930,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥330,000)
Fiscal Year 2007: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2006: ¥2,500,000 (Direct Cost: ¥2,500,000)
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| Keywords | atherosclerosis / Cl-channel / endothelial cells / amphotericin B / cvto-protection / ion channel / high NaC1 diet / Cl-channel blocker / 細胞障害 / 塩素シオン / 塩素イオン |
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| Research Abstract |
We established the primary culture of the endothelial cells from rabbits. We examined the role of ion channels in the cytoprotective effects. We applied amphotericin B on the endothelial cells which caused cell damage by the enhancement of the membrane permeability and tested different ionic conditions and ion channel blockers. We found that Cl- channel blockers, NPPB and DIDS, inhibited endothelial cell damage caused by amphotericin B. We also found that Cl- replacement by other organic anions suppressed cell-damage by amphotericin B. Cellular damage caused by hydrogen peroxide was also inhibited by Cl- channel blockers or Cl- free conditions. These results suggest that Cl- influx via Cl- channels might cause the endothelial cell damage during the formation of atherosclerosis. To access this question, we applied high NaC1 diet to the atherosclerosis model rabbit (KHC rabbit) for 6 months to check the formation of atherosclerosis. Surprisingly we found. that the formation of atherosclerosis was suppressed by the high NaC1 diet. We are investigating the mechanisms of this effect.
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