| Budget Amount *help |
¥1,150,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥150,000)
Fiscal Year 2007: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000)
Fiscal Year 2006: ¥500,000 (Direct Cost: ¥500,000)
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| Research Abstract |
ACh-induced membrane responses in vascular endothelial cells that have been reported vary between preparations from a sustained hyperpolarization to a transient hyperpolarization followed by a depolarization; the reason for this variation is unknown. Using the perforated of conventional whole-cell clamp technique, we investigated ACh-induced membrane currents in freshly isolated endothelial layers having a resting membrane potential of less negative than -10 mV. A group of cells was electrically isolated using a wide-bore micropipette, and their membrane potential was well controlled. ACh activated K^+ and Cl^- currents simultaneously. The K^+ current was blocked by a combination of charybdotoxin and apamin and appears to result from the opening of Ca^<2+> activated intermediate conductance potassium channels (IK_(Ca)) and small conductance potassium channels (SK_(Ca). The Cl^- current was partially blocked by tamoxifen, niflumic acid, or DIDS and appears to be produced by Ca^<2+> activated Cl^- channels. When the pipettes contained 20 mM Cl^-, the ACh-induced K^+ conductance started decreasing during a 1-min application of ACh while the Cl^-conductance continued, making the ACh-induced hyperpolarization sustained. When the pipettes contained 150 mM Cl^-, both conductances started decreasing during a 1-min application of ACh, making the ACh-induced hyperpolarization small and transient. [Cl-]_i, is very likely modified by experimental procedures such as the cell isolation and the intracellular dialysis with the pipette solution. Such a variability in [Cl-]_i, may be one of the reasons for the variations in the ACh-induced membrane response.
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