Physiological Significance of the T-type Ca channels in smooth muscle cells.

• Project/Area Number: 18590213
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: General physiology
• Research Institution: Fukuoka Dental College
• Principal Investigator: KITAMURA Kenji Fukuoka Dental College, 歯学部, 教授 (30112345)
• Co-Investigator(Renkei-kenkyūsha): OHKUBO Tsuyako 福岡歯科大学, 歯学部, 講師 (40099065)
• Project Period (FY): 2006 – 2008
• Project Status: Completed (Fiscal Year 2008)
• Budget Amount: ¥4,130,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥630,000); Fiscal Year 2008: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2007: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2006: ¥1,400,000 (Direct Cost: ¥1,400,000)
• Keywords: イオンチャネル / Caチャネル / シグナル伝達 / 電流 / 発現 / T型Caチャネル / クモ毒 / カルシウムチャネル / キメラチャネル / α1H / α1G / ProTX-I / 生理学 / 薬理学 / 細胞組織 / 循環器・高血圧 / 平滑筋 / 細胞・組織

Research Abstract

平滑筋細胞の収縮はCaチャネルから流れ込むCa^<2+>量に依存し、収縮に必要なCa流入に関与するL型とL型の制御を行うT型に分けられる。平滑筋細胞にはα1G及びα1Hの2種のT型Caチャネルが存在し、妊娠経過や病態によって発現量が変化する。両T型Caチャネルは近似したチャネル特性を持つが、クモ毒であるProTx-Iはα1Gに特異的な抑制を示す。このことから、両T型Caチャネルが共存することで、毒物等の侵入による機能不全を回避するシステムを構成していると考えられた。

Research Products

• [Journal Article] Molecular and immunohistochemical studies in expression of voltage-dependent Ca channels in dorsal root gangria from storeptozotocin-induced diabetic mice. (2006)
  • Author(s): Umeda M., Ohkubo T., Ono J., Fukuizumi T., Kitamura K.
  • Journal Title: Life Science 79 Pages: 1995-2000
• [Journal Article] Molecular and immunohistochemical studies in expression of voltage-dependent Ca channels in dorsal root gangria from storeptozotocin-induced diabetic mice (2006)
  • Author(s): Umeda M., Ohkubo T., Ono J., Fukuizumi T., Kitamura K.
  • Journal Title: Life Science 79 Pages: 1995-2000
• [Journal Article] Tarantula toxin ProTx-I differentiates between human T-type voltage-gated Ca^<2+> channels Cav3.1 and Cav3.2.
  • Author(s): Ohkubo, T., Kitamura K.
  • Journal Title: (submitted.) Pages: 0-0
  • NAID: 130000253386
• [Presentation] Tarantula toxin ProTx-1 differentiates between voltage-gated T-type Ca^<2+> channels hCav3.1 and hCav3.2. (2009)
  • Author(s): Ohkubo T., Kitamura K.
  • Organizer: 36th International Congress of Physiological Sciences
  • Place of Presentation: Kyoto, Japan
• [Presentation] Tarantula toxin ProTx-I differentiates between voltage-gated T-type Ca2+ channels hCav3.1 and hCav3.2. (2009)
  • Author(s): T. Ohkubo, K. Kitamura
  • Organizer: 36th International Congress of Physiological Sciences
  • Place of Presentation: Kyoto, japan
• [Presentation] The ProTx-1 receptor site of T-type Ca^<2+> channels. (2008)
  • Author(s): Ohkubo T.
  • Organizer: 日本薬理学会
  • Place of Presentation: 横浜
  • Year and Date: 2008-03-18
• [Presentation] The ProTX-I receptor site of T-type Ca2+ channels (2008)
  • Author(s): Ookubo Tsuyako
  • Organizer: 日本薬理学会
  • Place of Presentation: 横浜
  • Year and Date: 2008-03-18