| Project/Area Number |
18590222
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Environmental physiology (including Physical medicine and Nutritional physiology)
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| Research Institution | Siebold University of Nagasaki |
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Principal Investigator |
SURUGA Kazuhito Siebold University of Nagasaki, Faculty of Nursing and Nutritional, Associate Professor (70315852)
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| Project Period (FY) |
2006 – 2007
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| Project Status |
Completed (Fiscal Year 2007)
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| Budget Amount *help |
¥3,750,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥450,000)
Fiscal Year 2007: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2006: ¥1,800,000 (Direct Cost: ¥1,800,000)
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| Keywords | Vitamin A / β-carotene / HNF-4 / HNF-1 / small intestine / absorption / phosphorylation / C2BBe1 |
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| Research Abstract |
Human intestinal-like cell line C2BBe1 cells expressed higher levels of BCMO1, CRBPII and other vitamin A-related genes' transcripts than parental Caco-2 cells. Human CRBPII gene transcription was synergistically stimulated by HNF-4α and intestinal-specific transcription factors (CDX2 and GATAs). Human BCMO1 gene transcription was competitively regulated by HNF-4α and HNF-1α on the same distal promoter element. Moreover, PMA significantly decreased CRBPII and BCMO1 gene expression levels through PKC-ERK-mediated down-regulation of nuclear HNF-4α and HNF-1 levels in C2BBe1 cells. These transcription factors are also involved in the differentiation-induced vitamin A and β-carotene absorption and metabolism in the human small intestinal cells. In conclusion, HNF-4α and/or HNF-1α may be pivotal transcription factors involving in the bioavailability and homeostasis of human small intestinal vitamin A and β-carotene.
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