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Molecular and functional analysis of ATP-sensitive K^+ channel on the nuclear envelope

Research Project

Project/Area Number 18590232
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field General pharmacology
Research InstitutionChiba University

Principal Investigator

NAKAYA Haruaki  Chiba University, Graduate School of Medicine, Professor (60113594)

Co-Investigator(Kenkyū-buntansha) SATO Toshiaki  Chiba University, Graduate School of Medicine, Associate Professor (60244159)
OGURA Takehiko  Chiba University, Graduate School of Medicine, Assistant Professor (00292673)
Project Period (FY) 2006 – 2007
Project Status Completed (Fiscal Year 2007)
Budget Amount *help
¥4,010,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥510,000)
Fiscal Year 2007: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
Fiscal Year 2006: ¥1,800,000 (Direct Cost: ¥1,800,000)
Keywordsnuclear envelop / ATP-sensitive K^+ channel / sufonylurea drug / K^+ channel opener / Heart / 薬理学 / 生理学 / 細胞・組織
Research Abstract

This study was undertaken to test whether ATP-sensitive K^+ (K_<ATP> ) channel exists on the nuclear envelope of cardiac cells and to determine the functional molecules and physiological roles of K_<ATP> channel on the nuclear envelope. Using binding assay of glibenclamide-BODIPY-FL to sulfonylurea receptor we tested the existence of K_<ATP> channel on the nuclear envelope. We could observe the binding of glibenclamide-BODIPY-FL colocalized with Hoechst, a nuclear marker, using confocal microscopy, suggesting the existence of sufonylurea receptor on the nuclear envelope of cardiomyocytes. The single channel activity was recorded from the nuclear envelope of isolated nuclei using patch clamp techniques. Although several kinds of single channel activity could be recorded from the nuclear envelope, the channel activity was not influenced by glibenclamide, tolbutamide, or pinacidil. Nuclear Ca^<2+> signal was measured using confocal microscopy and the Ca^<2+> sensitive fluorescence dye Fluo-3/AM. Neither glibenclamide nor tolbutamide affected Ca^<2+> concentration in the nuclear envelope. Thus, even if K_<ATP> channel exists on the nuclear envelope of cardiac cells, the density or physiological significance of the channel may not be so large.

Report

(3 results)
  • 2007 Annual Research Report   Final Research Report Summary
  • 2006 Annual Research Report
  • Research Products

    (44 results)

All 2008 2007 2006

All Journal Article (28 results) (of which Peer Reviewed: 11 results) Presentation (13 results) Book (3 results)

  • [Journal Article] Infarct size limitation by adrenomedullin: protein kinase A but not PI3-kinase is linked to mitochondrial Kc_a channels.2008

    • Author(s)
      Nishida H.
    • Journal Title

      Cardiovasc. Res 77

      Pages: 398-405

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
    • Peer Reviewed
  • [Journal Article] Takayasu arteritis evaluated by multi-slice computed tomography in an old man.2008

    • Author(s)
      Fujita T.
    • Journal Title

      Int. J. Cardiol. 125

      Pages: 286-287

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
    • Peer Reviewed
  • [Journal Article] Conformational switch of angiotensin II type 1 receptor underlying mechanical stress-induced activation.2008

    • Author(s)
      Yasuda N.
    • Journal Title

      EMBO Rep. 9

      Pages: 179-186

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
    • Peer Reviewed
  • [Journal Article] Infarct size limitation by adrenomedullin: protein kinase a but not PI3-kinase is linked to mitochondrial Kc_a channels2008

    • Author(s)
      Nishida H.
    • Journal Title

      Cardiovasc. Res 77

      Pages: 398-405

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] Takayasu arteritis evaluated by multi-slice computed tomography in an old man2008

    • Author(s)
      Fujita T.
    • Journal Title

      Int. J. Cardiol 125

      Pages: 286-287

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] Conformational switch of angiotensin II type 1 receptor underlying mechanical stress-induced activation2008

    • Author(s)
      Yasuda N.
    • Journal Title

      EMBO Rep 9

      Pages: 179-186

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] Infarct size limitation by adrenomedullin:protein kinase A but not PI3-kinase is linked to mitochondrial K_ca channels2008

    • Author(s)
      Nishida H.
    • Journal Title

      Cardiovasc.Res 77

      Pages: 398-405

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Takayasu arteritis evaluated by multi-slice computed tomography in an old man2008

    • Author(s)
      Fujita T
    • Journal Title

      Int.J.Cardiol 125

      Pages: 286-287

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Conformational switch of angiotensin II type l receptor underlyingmechanlcal stress-induced activation2008

    • Author(s)
      Yasuda N.
    • Journal Title

      EMBO Rep 9

      Pages: 179-186

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Effects of azimilide on the muscarinic acetylcholine receptor-operated K^+ current and experimental atrial fibrillation in guinea-pig hearts.2007

    • Author(s)
      Nishida A.
    • Journal Title

      J. Pharmacol. Sci. 105

      Pages: 229-239

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
    • Peer Reviewed
  • [Journal Article] Effects of azimilide on the muscarinic acetylcholine receptor-operated K^+ current and experimental atrial fibrillation in guinea-pig hearts2007

    • Author(s)
      Nishida A.
    • Journal Title

      J. Pharmacol. Sci 105

      Pages: 229-239

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] Effects of azimilide on the muscarinic acetylcholine receptor-operated K^+current and experimental atrial fibrillation in guinea-pig hearts2007

    • Author(s)
      Nishida A.
    • Journal Title

      J.Pharmacol.Sci 105

      Pages: 229-239

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Journal Article] 抗心房細動薬の展望-新しい標的を求めて-2007

    • Author(s)
      中谷 晴昭
    • Journal Title

      Heart View 11

      Pages: 64-70

    • Related Report
      2007 Annual Research Report
  • [Journal Article] ATP感受性K^+チャネル2007

    • Author(s)
      中谷 晴昭
    • Journal Title

      日薬理誌 130

      Pages: 80-83

    • NAID

      10019813403

    • Related Report
      2007 Annual Research Report
  • [Journal Article] 抗血小板薬シロスタゾールの電気生理学的作用とその意義2007

    • Author(s)
      中谷 晴昭
    • Journal Title

      臨床と研究 84

      Pages: 113-118

    • Related Report
      2007 Annual Research Report
  • [Journal Article] 心房細動薬物治療の標的分子2007

    • Author(s)
      中谷 晴昭
    • Journal Title

      PM基礎・治療 27

      Pages: 43-47

    • Related Report
      2007 Annual Research Report
  • [Journal Article] KATPチャネルと血管攣縮2007

    • Author(s)
      中谷 晴昭
    • Journal Title

      医学のあゆみ 223

      Pages: 1146-1150

    • Related Report
      2007 Annual Research Report
  • [Journal Article] 心筋細胞K^+チャンネルおよびミトコンドリアに対するベプリジルの作用2007

    • Author(s)
      中谷晴昭
    • Journal Title

      心電図 27・1

      Pages: 21-29

    • Related Report
      2006 Annual Research Report
  • [Journal Article] A key role for the subunit SUR2B in the preferential activation of vascular K_<ATP> channels by isoflurane2006

    • Author(s)
      Fujita H.
    • Journal Title

      Br. J. Pharmacol. 149

      Pages: 573-580

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
    • Peer Reviewed
  • [Journal Article] Randomized study of angiotensin II type 1 receptor blocker vs dihydropydine calcium antagoist for the treatment of paroxysmal atrial fibrillation in patients with hypertentnsion. -The J-RHYTHM II study design for the investigation of upstream therapy for atrial fibrillation-.2006

    • Author(s)
      Yamashita Y.
    • Journal Title

      Circ. J. 70

      Pages: 1318-1321

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
    • Peer Reviewed
  • [Journal Article] Effects of sulfonylureas on mitochondrial ATP-sensitive K^+ channels in cardiac myocytes: implications for sulfonylurea controversy2006

    • Author(s)
      Sato T.
    • Journal Title

      Diabetes Metab Res Rev 22

      Pages: 341-347

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
    • Peer Reviewed
  • [Journal Article] A key role for the subunit SUR2B in the preferential activation of vascular K_<ATP> channels by isoflurane2006

    • Author(s)
      Fujita H.
    • Journal Title

      Br. J. Pharmacol 149

      Pages: 573-580

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] Randomized study of angiotensin II type 1 receptor blocker vs dihydropyridine calcium antagonist for the treatment of paroxysmal atrial fibrillation in patients with hypertentnsion.-The J-RHYTHM II study design for the investigation of upstream therapy for atrial fibrillation2006

    • Author(s)
      Yamashita Y
    • Journal Title

      Circ. J. 70

      Pages: 1318-1321

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] Effects of sulfonylureas on mitochondrial ATP-sensitive K^+ channels in cardiac myocytes: implications for sulfonylurea controversy2006

    • Author(s)
      Sato T.
    • Journal Title

      Diabetes Metab Res Rev. 22

      Pages: 341-347

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] A key role for the subunit SUR2B in the preferential activation of vascular K_ATP channels by isoflurane2006

    • Author(s)
      Fujita H.
    • Journal Title

      Br.J.Pharmacol. 149・5

      Pages: 573-580

    • Related Report
      2006 Annual Research Report
  • [Journal Article] Randomized study of angiotension II type 1 receptor blocker vs dihydropiridine calcium antagonist for the treatment of paroxysmal atrial fibrillation in patients with hypertentnsion. -The J-RHYTHM II studey design for the investigation of upstream therapy for atrial fibrillation-2006

    • Author(s)
      Yamashita Y.
    • Journal Title

      Circ.J. 70・10

      Pages: 1318-1321

    • Related Report
      2006 Annual Research Report
  • [Journal Article] Effects of sulfonylureas on mitochondrial ATP-sensitive K^+ channels in cardiac myocytes : implications for sulfonylurea controversy2006

    • Author(s)
      Sato T.
    • Journal Title

      Diabetes Metab Res Rev 22・5

      Pages: 341-347

    • Related Report
      2006 Annual Research Report
  • [Journal Article] 心不全に伴うイオンチャンネルのリモデリングとその治療戦略2006

    • Author(s)
      中谷晴昭
    • Journal Title

      医学のあゆみ 心不全UPDATE 218・14

      Pages: 1201-1206

    • Related Report
      2006 Annual Research Report
  • [Presentation] 薬物の催不整脈作用の評価とその意義2008

    • Author(s)
      中谷晴昭
    • Organizer
      第81回日本薬理学会年会
    • Place of Presentation
      横浜
    • Year and Date
      2008-03-17
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Annual Research Report 2007 Final Research Report Summary
  • [Presentation] Evaluation of arrhythmogenic properties of new drugs2008

    • Author(s)
      Nakaya H.
    • Organizer
      The 81st Annual Meeting of The Japanese Pharmacological Society
    • Place of Presentation
      Yokohama
    • Year and Date
      2008-03-17
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] ATP感受性K^+チャネルと不整脈2007

    • Author(s)
      中谷晴昭
    • Organizer
      循環器研究フォーラム
    • Place of Presentation
      つくば
    • Year and Date
      2007-11-01
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] Role of ATP-sensitive K^+ channels in genesis of arrhythmias2007

    • Author(s)
      Nakaya H.
    • Organizer
      Cardiovascular Research form
    • Place of Presentation
      Tukuba
    • Year and Date
      2007-11-01
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] マウス洞房結節細泡におけるATP感受性K^+チャネルの電気生理学的役割の解析2007

    • Author(s)
      福崎紘一
    • Organizer
      第24回日本心電学会学術集会
    • Place of Presentation
      名古屋
    • Year and Date
      2007-10-05
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] Electrophysiological roles of ATP-sensitive K^+ channels in mouse sinoatrial node2007

    • Author(s)
      Fukuzaki K.
    • Organizer
      The 24th Annual Meeting of the Japanese Society of Electrocardiol-ogy
    • Place of Presentation
      Nagoya
    • Year and Date
      2007-10-05
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] マウス洞房結節細胞におけるATP感受性K+チャネルの電気理学的役割の解析2007

    • Author(s)
      福崎 紘一
    • Organizer
      第24回日本心電学会学術集会
    • Place of Presentation
      名古屋
    • Year and Date
      2007-10-05
    • Related Report
      2007 Annual Research Report
  • [Presentation] Role of ATP-sensitive K^+ channels in cardioprotection2006

    • Author(s)
      中谷晴昭
    • Organizer
      2nd World Congress 2006 International Academy of Cardiovascular Sciences
    • Place of Presentation
      札幌
    • Year and Date
      2006-07-14
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] Role of ATP-sensitive K^+ channels in cardioprotection2006

    • Author(s)
      Nakaya H.
    • Organizer
      2nd World Congress 2006 Inter-national Academy of Cardiovascu-lar Sciences
    • Place of Presentation
      Sapporo
    • Year and Date
      2006-07-14
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] 心筋細胞K^+チャネルおよびミトコンドリアに対するベプリジルの作用2006

    • Author(s)
      中谷晴昭
    • Organizer
      第23回日本心電学会学術集会
    • Place of Presentation
      東京
    • Year and Date
      2006-07-07
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] Effect of bepridil on cardiac K^+ channels and mitochondria2006

    • Author(s)
      Nakaya H.
    • Organizer
      The 23th Annual Meeting of the Japanese Society of Electrocardiol-ogy
    • Place of Presentation
      Yokyo
    • Year and Date
      2006-07-07
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] 遺伝子欠損動物を用いた洞房結節細胞におけるATP感受性K^+チャネルの役割の解析2006

    • Author(s)
      福崎紘一
    • Organizer
      第114回日本薬理学会関東部会
    • Place of Presentation
      千葉
    • Year and Date
      2006-06-10
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] Analysis of pathophysiological role of ATP-sensitive K^+ channel in sinoatrial node cells using Kir6.2-deficient mice2006

    • Author(s)
      Fukuzaki K.
    • Organizer
      The 114th Kanto Area Regional Meeting of the Japanese Pharmacological Society
    • Place of Presentation
      chiba
    • Year and Date
      2006-06-10
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Book] QT間隔の診かた・考え方2007

    • Author(s)
      中谷 晴昭
    • Total Pages
      259
    • Publisher
      医学書院
    • Related Report
      2007 Annual Research Report
  • [Book] Medical Topics series不整脈20072007

    • Author(s)
      中谷 晴昭
    • Total Pages
      215
    • Publisher
      メディカルレビュー社
    • Related Report
      2007 Annual Research Report
  • [Book] (訳)カラー図解 これならわかる薬理学 Color Altas of Pharmacology 3^rd edition, revised and expanded.2006

    • Author(s)
      佐藤俊明
    • Total Pages
      381
    • Publisher
      メディカル・サイエンス・インターナショナル
    • Related Report
      2006 Annual Research Report

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Published: 2006-04-01   Modified: 2016-04-21  

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