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The Regulatory Mechanism of Differentiation and Growth by Prostaglandin D2 in Pigment Cells

Research Project

Project/Area Number 18590254
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field General medical chemistry
Research InstitutionTohoku University

Principal Investigator

TAKEDA Kazuhisa  Tohoku University, Tohoku University, School of Medicine, Research Associate (30311559)

Co-Investigator(Kenkyū-buntansha) SHIBAHARA Shigeki  Tohoku University, School of Medicine, Professor (70206142)
Project Period (FY) 2006 – 2007
Project Status Completed (Fiscal Year 2007)
Budget Amount *help
¥4,020,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥420,000)
Fiscal Year 2007: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2006: ¥2,200,000 (Direct Cost: ¥2,200,000)
KeywordsMITF / Prostaglandin D synthase / melanocytes / transcription factor / Prostaglandin D2 / melanoma / tumor suppressor gene / growth
Research Abstract

Microphthalmia-associated transcription factor (Mitf) is responsible for differentiation of melanocytes. The black-eyed white (Mi-bw) mouse, a recessive Mitf mutant, lacks melanocytes in hair follicles and inner ear due to the impaired expression of Mitf, thereby exhibiting white coat color and deafness. By cDNA microarray analysis between wild type and Mi-bw mouse skin, we have identified lipocalin-type prostaglandin D synthase (L-PGDS) as a new melanocyte marker. L-PGDS exerts various biological actions in part via production of prostaglandin D_2 (PGD_2). Interestingly L-PGDS is expressed in normal human epidermal melanocytes, but not in human melanoma cell lines, as judged by northern blot and RT-PCR analyses. Such unexpected findings prompted us to examine whether L-PGDS is a potential tumor suppressor. Accordingly, we examined the effect of PGD_2 on the cell growth of human melanoma cells, using HMV-II, SK-MEL-28, 624mel, and G361 cell lines. The growth of melanoma cells was significantly repressed by the treatment with PGD_2, but not with each agonist specific for the PGD_2 receptor, DP1 or DP2, suggesting that PGD_2 may repress the growth of melanoma cells through a mechanism independent of the known PGD_2 receptor. Thus, the loss of L-PGDS expression in melanoma cells may be beneficial for their growth. These results suggest that L-PGDS may function as a potential tumor suppressor in human melanoma cells.

Report

(3 results)
  • 2007 Annual Research Report   Final Research Report Summary
  • 2006 Annual Research Report
  • Research Products

    (15 results)

All 2007 2006 Other

All Journal Article (10 results) (of which Peer Reviewed: 5 results) Presentation (4 results) Remarks (1 results)

  • [Journal Article] Neuroendocrine functions of melanocytes: Beyond the skin-deep melanin marker2007

    • Author(s)
      Takeda, Kazuhisa
    • Journal Title

      Tohoku Journal of Experimental Medicine 211

      Pages: 201-221

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
    • Peer Reviewed
  • [Journal Article] Augmented chemosensitivity in black-eyed white Mitfmi-bw mice, lacking melanocytes.2007

    • Author(s)
      Takeda, Kazuhisa
    • Journal Title

      Journal of Biochemistory (Tokyo) 141・3

      Pages: 327-333

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
    • Peer Reviewed
  • [Journal Article] Neuroendocrine functions of melanocytes: Beyond the skin-deep melanin marker2007

    • Author(s)
      Takeda K et. al.
    • Journal Title

      Tohoku Journal of Experimental Medicine 211

      Pages: 201-221

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] Augmented chemosensitivity in black-eyed white Mitftni-bw mice, lacking melanocytes2007

    • Author(s)
      Takeda K et. al.
    • Journal Title

      Journal of Biochemistory (Tokyo) 141-3

      Pages: 327-333

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] Neuroendocrine functions of melanocytes: Beyond the skin-deep melanin marker2007

    • Author(s)
      Takeda, Kazuhisa
    • Journal Title

      Tohoku Journal of Experimental medicine 211

      Pages: 201-221

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Augmented chemosensitivity in black-eyed white Mitfmi-bw mice, lacking melanocytes.2007

    • Author(s)
      Takeda, Kazuhisa
    • Journal Title

      Journal of Biochemistory(Tokyo) 141・3

      Pages: 327-333

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Neuroendocrine functions of melanocytes : Beyond the skin-deepmelanin marker2007

    • Author(s)
      Takeda K et al.
    • Journal Title

      Tohoku Journal of Experimental medicine 211

      Pages: 201-221

    • Related Report
      2006 Annual Research Report
  • [Journal Article] Augmented chemosensitivity in black-eyed white Mitfmi-bw mice, lacking melanocytes.2007

    • Author(s)
      Takeda K et al.
    • Journal Title

      Journal of Biochemistory (Tokyo) 141・3

      Pages: 327-333

    • Related Report
      2006 Annual Research Report
  • [Journal Article] Lipocalin-type prostaglandin D synthase as a melanocyte marker regulated by MITF2006

    • Author(s)
      Takeda, Kazuhisa
    • Journal Title

      Biochem. Biophys. Res. Commun. 339

      Pages: 1098-1106

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
    • Peer Reviewed
  • [Journal Article] Lipocalin-type prostaglandin D synthase as a melanocyte marker regulated by MITF2006

    • Author(s)
      Takeda K et. al.
    • Journal Title

      Biochem. Biophys. Res. Commun 339

      Pages: 1098-1106

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] PROSTAGLANDIN D_2 REPRESSES THE GROWTH OF HUMAN MELANOMA CELLS; REVISITED WITH MI-BW MOUSE2007

    • Author(s)
      Takeda, Kazuhisa
    • Organizer
      第21回日本色素細胞学会年次学術大会
    • Place of Presentation
      藤田保健衛生大学
    • Year and Date
      2007-12-08
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Annual Research Report 2007 Final Research Report Summary
  • [Presentation] PROSTAGLANDIN D_2 REPRESSES THE GROWTH OF HUMAN MELANOMA CELLS; REVISITED WITH MI-BW MOUSE2007

    • Author(s)
      Takeda K
    • Organizer
      The 21^<st> Annual meeting of the Japanese Society for Pigment Cell Research
    • Place of Presentation
      Fujita Health Science University, Toyoake city, Japan
    • Year and Date
      2007-12-08
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] Lipocalin-type prostaglandin D synthase as a Potential tumor suppressor in human melanoma cells2006

    • Author(s)
      Takeda, Kazuhisa
    • Organizer
      International Union of Biochemistry & Molecular Biology 2006
    • Place of Presentation
      横浜パシフィコ
    • Year and Date
      2006-06-22
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] Lipocalin-type prostaglandin D synthase as a potential tumor suppressor in human melanoma cells2006

    • Author(s)
      Takeda K
    • Organizer
      International Union of Biochemistry and Molecular Biology 2006
    • Place of Presentation
      Pacifico Yokohama
    • Year and Date
      2006-06-22
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Remarks]

    • URL

      http://www.mbap.med.tohoku.ac.jp

    • Related Report
      2007 Annual Research Report

URL: 

Published: 2006-04-01   Modified: 2016-04-21  

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