The establishment of telomerase control technology and applicationfor medical regeneration

• Project/Area Number: 18590265
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: General medical chemistry
• Research Institution: Osaka University
• Principal Investigator: ISHIKAWA Kazuhiko Osaka University, 医学部・附属病院, 助教 (60379245)
• Co-Investigator(Kenkyū-buntansha): RAKUGI Hiromi 大阪大学, 大学院・医学系研究科, 教授 (20252679) ; OGIHARA Toshio 大阪大学, 大学院・医学系研究科, 名誉教授 (60107042)
• Project Period (FY): 2006 – 2008
• Project Status: Completed (Fiscal Year 2008)
• Budget Amount: ¥4,100,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥600,000); Fiscal Year 2008: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2007: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2006: ¥1,500,000 (Direct Cost: ¥1,500,000)
• Keywords: hTERT / テロメア / 血管内皮細胞 / 老化 / テロメラーゼ

Research Abstract

長期発現ベクターpEBcにテロメラーゼサブユニットであるhTERT遺伝子を組み込み、血管内皮細胞に導入する事で、長期に遺伝子発現が維持され、強い抗老化、抗酸化ストレス作用を有する細胞を得た。またhTERT遺伝子が、テロメラーゼ活性の変化を介さずに、直接的に細胞接着因子の発現やNF-κB活性を上昇させ、抗アポトーシス効果を発揮している事が明らかとなった。

Research Products

• [Journal Article] Klotho Suppresses TNF-a-Induced Expression of Adhesion Molecules in the Endothelium and Attenuates NF-κB Activation (2009)
  • Author(s): Maekawa Y, Ishikawa K, Rakugi H et.al.
  • Journal Title: Endocrine (in press)
  • Peer Reviewed
• [Journal Article] Akt activation prevents Apop-1 induced death of cells. (2008)
  • Author(s): Sun X, et al
  • Journal Title: Biochem Biophys Res Commun. 377(4) Pages: 1097-1101
  • Peer Reviewed
• [Journal Article] Anti-oxidative effect of Klotho on endothelial cells through cAMP activation (2007)
  • Author(s): H Rakugi, Ishikawa K, Ogihara T et.al.
  • Journal Title: Endocrine 31(1) Pages: 82-87
  • Peer Reviewed
• [Journal Article] Klotho gene delivery suppresses oxidative stress in vivo (2007)
  • Author(s): Ohta J, Rakugi H, Ishikawa K, Ogihara T et.al.
  • Journal Title: Geriatrics & Gerontology International 7(3) Pages: 293-299
  • NAID: 10020021335
  • Peer Reviewed
• [Journal Article] Klotho gene delivery suppresses oxidative stress in vivo (2007)
  • Author(s): J Ohta, et. al.
  • Journal Title: Geriatrics & Gerontology International 7(3) Pages: 293-299
  • Peer Reviewed
• [Journal Article] Anti-oxidative effect of Klotho on endothelial cells through cAMP activation (2007)
  • Author(s): H Rakugi, et. al.
  • Journal Title: Endocrine 31(1) Pages: 82-87
  • Peer Reviewed
• [Journal Article] Klotho protein promotes adipocyte differentiation (2006)
  • Author(s): Chihara Y, Rakugi H, Ishikawa K, Ogihara T et.al.
  • Journal Title: Endocrinology 147(8) Pages: 3835-3842
  • Peer Reviewed
• [Journal Article] Anti-apoptotic and anti-senescence effects of Klotho on vascular endothelial cells (2006)
  • Author(s): Ikushima M, Rakugi H, Ishikawa K, Ogihara T et.al.
  • Journal Title: Biochem Biophys Res Commun 339(3) Pages: 827-832
  • Peer Reviewed
• [Journal Article] Klotho protein promotes adipocyte differentiation. (2006)
  • Author(s): Chihara Y, Ishikawa K et al.
  • Journal Title: Endocrinology 147(8) Pages: 3835-3842
• [Journal Article] Anti-apoptotic and anti-senescence effects of Klotho on vascular endothelial cells. (2006)
  • Author(s): Ikushima M, Ishikawa K et al.
  • Journal Title: Biochem Biophys Res Commun 339(3) Pages: 827-832
• [Presentation] 血管内皮細胞におけhTERTの抗アポトーシス作用 (2007)
  • Author(s): 木田岩男
  • Organizer: 第49回日本老年医学会学術集会
  • Place of Presentation: 札幌
  • Year and Date: 2007-06-22
• [Presentation] 血管内皮細胞におけるhTERTの抗アポトーシス作用 (2007)
  • Author(s): 木田 岩男
  • Organizer: 第49回日本老年医学会学術集会
  • Place of Presentation: 札幌、北海道
  • Year and Date: 2007-06-22
• [Presentation] The effect of aging and oxidative stress on hTERT expression of vascular endothelial cells (2006)
  • Author(s): Ishikawa K
  • Organizer: The 21^<st> Scientific Meeting of the International Society of Hypertension
  • Place of Presentation: 福岡
  • Year and Date: 2006-10-17
• [Presentation] 血管内皮細胞におけるhTERTの新規機能解明 (2006)
  • Author(s): 石川一彦
  • Organizer: 日本老年医学会学術総会
  • Place of Presentation: 金沢
  • Year and Date: 2006-06-09