| Project/Area Number |
18590273
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
General medical chemistry
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| Research Institution | Nagasaki University |
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Principal Investigator |
KISHINO Tatsuya Nagasaki University, Center for Frontier Life Sciences, Division of Functional Genomics, Associate Professor (70315232)
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| Project Period (FY) |
2006 – 2007
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| Project Status |
Completed (Fiscal Year 2007)
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| Budget Amount *help |
¥4,070,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥570,000)
Fiscal Year 2007: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
Fiscal Year 2006: ¥1,600,000 (Direct Cost: ¥1,600,000)
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| Keywords | imprinting / epigenetics / DNA methvyation / histone modifications / neuron / Ube3a / 神経細胞 / MeCP2 |
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| Research Abstract |
Most imprinted genes display parental-origin-specific gene expression in all tissue where they are transcribed, however some genes are imprinted in a tissue-specific manner. Genes that show brain-specific imprinting or brain-specific lack of imprinting present a unique opportunity to study the process of imprinting during tissue differentiation. In this project, I analyzed brain cell lineage-specific imprinting using a primary brain cell culture system and discussed imprinting-associated epigenetic modifications in neurons. I focused on the imprinted genes, Grb10, and Snrpn-Ube3a, which. show neuron-specific imprinting and analyzed DNA methylation, histone modifications and antisense RNA.
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