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An analysis of the role of protein kinase PKN3 in the progress of cancer and a development of new anticancer agent

Research Project

Project/Area Number 18590294
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Pathological medical chemistry
Research InstitutionKobe University

Principal Investigator

MUKAI Hideyuki  Kobe University, Biosignal Research Center, Associate Professor (80252758)

Project Period (FY) 2006 – 2007
Project Status Completed (Fiscal Year 2007)
Budget Amount *help
¥4,020,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥420,000)
Fiscal Year 2007: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2006: ¥2,200,000 (Direct Cost: ¥2,200,000)
KeywordsRho / small GTPase / PKN3 / protein kinase / cancer / oncogenesis / knockout mice
Research Abstract

We prepared protein kinase PKN3 knockout mice to analyze the function of PKN3 in vivo. PKN3 knockout mice did not show any significant macroscopic morphological abnormalities and problems in reproductive potential and the feeding behavior. We isolated embryonic fibroblasts from PKN3 knockout mice and examined the wound healing activity and RasV12 induced focus forming activity, comparing with the wild type mice. And we crossed PKN3 knockout mice with pten conditional knockout mice which are susceptible to malignant neoplasms, and examined the effect of PKN3 on the tumorigenesis and metastasis. In order to prepare a specific inhibitor for PKN3, we prepared various deletion mutants and point mutants of PKN3 and analyzed the activation mechanism of PKN3 by checking the protein phosphorylation activity of the enzyme. We also checked the effect of PDK1 on the stability and the activation mechanism of PKN3.

Report

(3 results)
  • 2007 Annual Research Report   Final Research Report Summary
  • 2006 Annual Research Report
  • Research Products

    (18 results)

All 2008 2007 2006 Other

All Journal Article (8 results) (of which Peer Reviewed: 4 results) Presentation (5 results) Book (3 results) Remarks (2 results)

  • [Journal Article] Calcineurin-mediated dephosphorylation of c-Jun Ser-243 is required for c-Jun protein stability and cell transformation2008

    • Author(s)
      Huang CC
    • Journal Title

      Oncogene 27

      Pages: 2422-2429

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Calcineurin-mediated dephosphorylation of c-Jun Ser-243 is required for c-Jun protein stability and cell transformation2007

    • Author(s)
      Huang CC
    • Journal Title

      Oncogene 27号

      Pages: 2422-2429

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
    • Peer Reviewed
  • [Journal Article] Fragmentation of Protein Kinase N (PKN) in the Hydrocephalic Rat Brain. Acta Histochem Cytochem.2007

    • Author(s)
      Okii N
    • Journal Title

      Acta Histochem Cytochem. 40

      Pages: 113-121

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
    • Peer Reviewed
  • [Journal Article] Fragmentation of Protein Kinase N(PKN)in the Hydrocephalic Rat Brain2007

    • Author(s)
      Okii, N, Amano, T, Seki, T, Matsubayashi, H, Mukai, H, Ono, Y, Kurisu, K, Sakai, N
    • Journal Title

      Acta Histochem Cytochem 40

      Pages: 113-21

    • NAID

      110006633421

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] Fragmentation of Protein Kinase N(PKN)in the Hydrocephalic Rat Brain. Acta Histochem Cytochem.2007

    • Author(s)
      Okii N
    • Journal Title

      Acta Histochem Cytochem. 40

      Pages: 113-121

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Purification and Kinase assay of PKN2006

    • Author(s)
      Mukai, H.
    • Journal Title

      Methods in Enzymology 406

      Pages: 234-250

    • NAID

      120000946410

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary 2006 Annual Research Report
  • [Journal Article] Purification, kinase assay of PKN2006

    • Author(s)
      Mukai, H, Ono, Y
    • Journal Title

      Methods Enzymol 406

      Pages: 234-50

    • NAID

      120000946410

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] Calcineurin-mediated dephosphorylation of c-Jun Ser-243 is required for c-Jun protein stability and cell transformation

    • Author(s)
      Huang, CC, Wang, JM, Kikkawa, U, Mukai, H, Shen, MR, Morita, I, Chen, BK, Chang, WC
    • Journal Title

      Oncogene(2007) (in press)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] PKN特異的認識モチーフの同定とPKNリン酸化活性阻害ペプチドの開発2007

    • Author(s)
      志賀一博
    • Organizer
      BMB2007
    • Place of Presentation
      パシフィコ横浜
    • Year and Date
      2007-12-11
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Annual Research Report 2007 Final Research Report Summary
  • [Presentation] バイオアップデート-Web2.0自律更新型日本語オープンアクセスジャーナルの公開と研究者口コミ情報の共有-2007

    • Author(s)
      向井秀幸
    • Organizer
      BMB2007
    • Place of Presentation
      パシフィコ横浜
    • Year and Date
      2007-12-11
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] An identification of the substrate recognition motif and a development of inhibitory peptide for PKN2007

    • Author(s)
      Shiga, K, Ueki, K, Hutti, J, Maeda, T, Turk, B, Ono, Y, Mukai, H
    • Organizer
      BMB2007
    • Place of Presentation
      Pacifico Yokohama
    • Year and Date
      2007-12-11
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] A Web 2.0 self-revisable Japanese open access journal and a shari ng of buzz information among biologists2007

    • Author(s)
      Mukai, H, Kuno, Y
    • Organizer
      BMB2007
    • Place of Presentation
      Pacifico Yokohama
    • Year and Date
      2007-12-11
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] バイオアップデートー-Web2.0自律更新型日本語オープンアクセスジャーナルの公開と研究者口コミ情報の共有-2007

    • Author(s)
      向井秀幸
    • Organizer
      2007年12月11日
    • Place of Presentation
      パシフィコ横浜
    • Year and Date
      2007-12-11
    • Related Report
      2007 Annual Research Report
  • [Book] ゲノム科学2008

    • Author(s)
      向井秀幸
    • Publisher
      京都廣川書店(in press)
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Book] Genome Science2008

    • Author(s)
      Mukai, H., et. al.
    • Publisher
      Kyoto Hirokawa Publishig Inc(in press)
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Book] ゲノム科学2008

    • Author(s)
      向井秀幸
    • Publisher
      京都廣川書店(In press)
    • Related Report
      2007 Annual Research Report
  • [Remarks] 「研究成果報告書概要(和文)」より

    • URL

      http://biouodate.jp

    • Related Report
      2007 Final Research Report Summary
  • [Remarks]

    • URL

      http://bioupdate.jp

    • Related Report
      2007 Annual Research Report

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Published: 2006-04-01   Modified: 2016-04-21  

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