• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

Elucidation of physiological finction of anandamide, an endogenous marijuana-like substance, in mammals

Research Project

Project/Area Number 18590297
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Pathological medical chemistry
Research InstitutionKanazawa University (2007)
Kagawa University (2006)

Principal Investigator

OKAMOTO Yasuo  Kanazawa University, Graduate Schcool of Medicine, Associate Professor (80293877)

Co-Investigator(Kenkyū-buntansha) UEKI Masaaki  Kagawa University, School of Medicine, Associate Professor (20213332)
UENO Masaki  Kagawa University, School of Medicine, Associate Professor (30322267)
Project Period (FY) 2006 – 2007
Project Status Completed (Fiscal Year 2007)
Budget Amount *help
¥3,690,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2007: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2006: ¥2,000,000 (Direct Cost: ¥2,000,000)
KeywordsN-Acylethanolamine / N-Acvlphosphatidvlethanolamine / Anandaroide / Endocannobinoid / Phospholipase D
Research Abstract

N-Acylethanolamines (NAEs) constitute a large and diverse class of signaling lipids that includes the endogenous cannabinoid anandamide. It is widely accepted that in mammalian tissues all NAEs are principally released from their corresponding N-acyl phosphatidylethanolamines (NAPEs) by the catalysis by a membrane-associated phospholipase D generally abbreviated as NAPE-PLD. Recently, NAPE-PLD was identified as a candidate enzyme involved in the biosynthesis of NAEs by us. To understand the physiological and pathophysiological significance of anandamide and other bioactive NAEs in mammals, we generated and characterized mice with a targeted disruption in the NAPE-PLD gene (NAPE-PLD-1). (1) lb generate mice lacking NAPE-PLD, exon 3 of the NAPE-PLD gene was removed by Cre/loxP system. (2) NAPE-PLD-1- were born at the expected Mendelian frequency, were viable and healthy, and showed no overt differences in their cage behavior compared to wild type littermates. (3) RT-PCR and Western blotting with anti-NAPE-PLD antibodies confirmed the absence of NAPE-PLD mRNA and protein in tissues from NAPE-PLO-h. (4) Tissues from NAPE FED" showed significantly lower NAPE-PLD activity with an N-palmitoyl-PE as substrate compared to wild type tissues. (5) Significant reductions in the levels of saturated NAEs were observed in NAPE-PLO-I brains. On the other hand, complementary profiles of NAPEs were found in NAPE-PLD+/+ and NAPE-PLD-1- brains, with the latter samples possessing 15-20-fold higher levels of saturated N-acyl NAPEs, These data suggest that NAPE-PLD is a principal enzyme responsible for the conversion of NAPEs to NAEs in mammals.

Report

(3 results)
  • 2007 Annual Research Report   Final Research Report Summary
  • 2006 Annual Research Report
  • Research Products

    (73 results)

All 2008 2007 2006 Other

All Journal Article (25 results) (of which Peer Reviewed: 14 results) Presentation (48 results)

  • [Journal Article] The expression of osteopontin is increased in vessels with blood-brain barrier impairment2008

    • Author(s)
      Iwanaga, Y.
    • Journal Title

      Neuropathl. Appl. Neurobiol. 34

      Pages: 145-154

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
    • Peer Reviewed
  • [Journal Article] The stimulatory effect of phosphatidylethanolamine on N-acylphosphatidylethanolamine-hydrolyzing phospholipase D(NAPE-PLD).2008

    • Author(s)
      Wang, J.
    • Journal Title

      Neuropharmacology 54

      Pages: 8-15

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
    • Peer Reviewed
  • [Journal Article] The expression of osteopontin is increased in vessels with blood-brain barrier impairment2008

    • Author(s)
      Iwanaga, Y
    • Journal Title

      Neuropathl. Appl. Neurobiol 34

      Pages: 145-154

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] The stimulatory effect of phosphatidylethanolamine on N-acylphosphatidylethanolamine-hydrolyzing phospholipase D (NAPE-PLD)12008

    • Author(s)
      Wang, J
    • Journal Title

      Neuropharmacology 54

      Pages: 8-15

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] The stimulatory effect of phosphatidylethanolamine on N-acylphosphatidylethanolamine-hydrolyzing phospholipase D (NAPE-PLD)2008

    • Author(s)
      Wang, J.
    • Journal Title

      Neuropharmacology 54

      Pages: 8-15

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Journal Article] The expression of osteopontin is increased in vessels with blood-brain barrier impairment2008

    • Author(s)
      Iwanaga, Y.
    • Journal Title

      Neuropathl.Appl.Neurobiol. 34

      Pages: 145-154

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Discovery and characterization of a Ca^<2+>-independent phosphatidylethanolamine N-acyltransferase generating the anandamide precursor and its congeners2007

    • Author(s)
      Jin, X.-H.
    • Journal Title

      J. Biol. Chem. 282

      Pages: 3614-3623

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
    • Peer Reviewed
  • [Journal Article] Proteolytic activation and plycosylation of N-acylethanolamine-hydrolyzing acid amidase, a lysosomal enzyme involved in the endocannabinoid metabolism.2007

    • Author(s)
      Zhao, L.-Y.
    • Journal Title

      Biochim. Biophys. Acta 1771

      Pages: 1397-1405

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
    • Peer Reviewed
  • [Journal Article] Biosynthetic pathways of the endocannabinoid anandamide.2007

    • Author(s)
      Okamoto, Y.
    • Journal Title

      Chem. Biodivers. 4

      Pages: 1842-1857

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
    • Peer Reviewed
  • [Journal Article] Predominant expression of lysosomal N-acylethanolamine-hydrolyzing acid amidase in macrophages revealed by immunochemical studies.2007

    • Author(s)
      Tsuboi, K.
    • Journal Title

      Biochim. Biophys. Acta 1771

      Pages: 623-632

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
    • Peer Reviewed
  • [Journal Article] Accumulation of triosephosphate isomerase, with sequence homology to Beta amyloid peptides, in vessel walls of the newborn piglet hippocampus.2007

    • Author(s)
      Kusaka, T.
    • Journal Title

      Microsc. Res. Tech. 70

      Pages: 648-655

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
    • Peer Reviewed
  • [Journal Article] Discovery and characterization of a Ca2+independent phosphatidylethanolamine N-acyltransferase generating the anandamide precursor and its congeners2007

    • Author(s)
      Jin, X. H
    • Journal Title

      J. Biol. Chem 282

      Pages: 3614-3623

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] Proteolytic activation and glycosylation of N-acylethanolamine-hydrolyzing acid amidase, a lysosomal enzyme involved in the endocannabinoid metabolism2007

    • Author(s)
      Zhao, L.Y
    • Journal Title

      Biochim. Biophys. Acta 1771

      Pages: 1397-1405

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] Biosynthetic pathways of the endocannabinoid anandamide2007

    • Author(s)
      Okamoto, Y
    • Journal Title

      Chem. Biodivers 4

      Pages: 1842-1857

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] Predominant expression of lysosomal N-acylethanolamine-hydrolyzing acid amidase in macrophages revealed by immunochemical studies2007

    • Author(s)
      Tsuboi, K
    • Journal Title

      Biochim. Biophys. Acta 1771

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] Accumulation of triosephosphate isomerase, with sequence homology to Beta amyloid peptides, in vessel walls of the newborn Piglet hippocampus2007

    • Author(s)
      Kusaka, T
    • Journal Title

      Microsc. Res. Tech 70

      Pages: 648-655

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] Proteolytic activation and glycosylation of N-acylethanolamine-hydrolyzing acid amidase, alysosomal enzyme involved in the endocannabinoid metabolism2007

    • Author(s)
      Zhao, L.Y.
    • Journal Title

      Biochim.Biophys.Acta 1171

      Pages: 1397-1405

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Biosynthetic pathways of the endocannabinoid anandamide2007

    • Author(s)
      Okamoto, Y.
    • Journal Title

      Chem.Biodivers 4

      Pages: 1842-1857

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Predominant expression of lysosomal N-acylethanolamine-hydrolyzing acid amidase in macrophages recealed by immunochemical studies2007

    • Author(s)
      Tsuboi, K.
    • Journal Title

      Biochim.Biophys.Acta 1771

      Pages: 623-632

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Accumulation of triosephosphate isomerase, with sequence homology to Beta amyloid peptides, in vessel walls of the newboron piglet hippocampus2007

    • Author(s)
      Kusaka, T.
    • Journal Title

      Microsc.Res.Tech. 70

      Pages: 648-655

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Discovery and characterization of a Ca^<2+>-independent phosphatidylethanolamine N-acyltransferase generating the anandamide-precursor and its congeners2007

    • Author(s)
      Jin, X.-H., et al.
    • Journal Title

      Journal of Biological Chemistry 282・6

      Pages: 3614-3623

    • Related Report
      2006 Annual Research Report
  • [Journal Article] Predominant expression of lysosomal N-acylethanolamine-hydrolyzing acid amidase in macrophages revealed by immunochemical studies2007

    • Author(s)
      Tsuboi, K. et al.
    • Journal Title

      Biochimica et Biophysica Acta (in press)

    • Related Report
      2006 Annual Research Report
  • [Journal Article] Functional analysis of the purified anandamide-generating phospholipase D as a member of the metallo-β-lactamase family2006

    • Author(s)
      Wang, J.
    • Journal Title

      J. Biol. Chem. 281

      Pages: 12325-12335

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
    • Peer Reviewed
  • [Journal Article] Functional analysis of the purified anandamide -generating phospholipase D as a member of the metallo-B-lactamase family2006

    • Author(s)
      Wang, J
    • Journal Title

      J. Biol. Chem 281

      Pages: 12325-12335

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] Functional analysis of the purified anandamide-generating phospholipase Das a member of the metallo-β-lactamase family2006

    • Author(s)
      Wang, J., et al.
    • Journal Title

      Journal of Biological Chemistry 281・18

      Pages: 12325-12335

    • Related Report
      2006 Annual Research Report
  • [Presentation] 新規リソソーム酵素としてのN-アシルエタノールアミン水解酸性アミダーゼの性状解析2007

    • Author(s)
      上田夏生
    • Organizer
      第318回脂溶性ビタミン総合研究委員会
    • Place of Presentation
      香川県さぬき市
    • Year and Date
      2007-12-17
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Annual Research Report 2007 Final Research Report Summary
  • [Presentation] Characteization of N-acylethanolamine -hydrolyzing acid amidase as a novel lysosomal enzyme2007

    • Author(s)
      Ueda, N
    • Organizer
      The 318th The Meeting of fat-soluble Vitamin
    • Place of Presentation
      Sanuki, Kagawa
    • Year and Date
      2007-12-07
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] アナンダミド前駆体N-アシルホスファチジルエタノールアミンのマクロファージ貪食抑制効果2007

    • Author(s)
      一木万奈美
    • Organizer
      第25回日本生化学会北陸支部例会
    • Place of Presentation
      金沢市
    • Year and Date
      2007-05-26
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] Inhibitatory effect of N-acylphosphatidylethanolamine on macrophage phagocytosis2007

    • Author(s)
      Ichiki, M
    • Organizer
      The 25th Hokuriku branch of the Japanese Biochemical Society
    • Place of Presentation
      Kanazawa
    • Year and Date
      2007-05-26
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] アナンダミド前駆体N-アシルボスファチジルエタノールアミンのマクロファージ貪食抑制効果2007

    • Author(s)
      一木万奈美
    • Organizer
      第25回日本生化学会北陸支部例会
    • Place of Presentation
      金沢市
    • Year and Date
      2007-05-26
    • Related Report
      2007 Annual Research Report
  • [Presentation] レシチン・レチノール・アシルトランスフェラーゼにホモロジーを示す機能未知のタンパク質はホスファチジルエタノールアミンN-アシルトランスフェラーゼとして作用する2007

    • Author(s)
      上田夏生
    • Organizer
      第315回脂溶性ビタミン総合研究委員会
    • Place of Presentation
      東京都
    • Year and Date
      2007-03-16
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] The protein with a homology to lecithin-retinol acyltransferase functions as a phosphatidylethanolamine N-acyltransferase2007

    • Author(s)
      Ueda, N
    • Organizer
      The 312th The Meeting of the fat-soluble Vitannia
    • Place of Presentation
      Tokyo
    • Year and Date
      2007-03-16
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] 内因性マリファナ様物質アナンダミドの生合成に係わる新規N-アシル転移酵素の同定と性状解析2007

    • Author(s)
      金星華
    • Organizer
      第48回日本生化学会中国・四国支部例会
    • Place of Presentation
      高知市
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] N-アシルPEを生成する新規N-アシル転移酵素の同定と性状解析2007

    • Author(s)
      岡本安雄
    • Organizer
      第49回日本脂質生化学会
    • Place of Presentation
      札幌市
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Annual Research Report 2007 Final Research Report Summary
  • [Presentation] Discovery of a calcium-independent phosphatidylethanolamine N-acyltransferase2007

    • Author(s)
      Ueda, N.
    • Organizer
      International Cannabinoid Research Society 17th Annual Symposium on the Cannabinoids
    • Place of Presentation
      Saint-Sauveur, Quebec, Canada
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] Immunochemical studies reveal predominant expression of lysosomal N-acylethanolamine-hydrolyzing acid amidase in macrophages2007

    • Author(s)
      Tsuboi, K.
    • Organizer
      International Cannabinoid Research Society 17th Annual Symposium on the Cannabinoids
    • Place of Presentation
      Saint-Sauveur, Quebec, Canada
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] 抗炎症脂質N-アシルエタノールアミンを加水分解する酸性アミダーゼのマクロファージにおける発現2007

    • Author(s)
      上田夏生
    • Organizer
      第28回日本炎症・再生医学会
    • Place of Presentation
      東京都新宿区
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] レシチン・レチノール・アシル転移酵素構造類似タンパク質のホスファチジルエタノールアミンN-アシル転移酵素としての同定2007

    • Author(s)
      金星華
    • Organizer
      第30回日本分子生物学会年会・第80回日本生化学会大会合同大会
    • Place of Presentation
      横浜市
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] 生体膜成分とリン脂質によるN-アシルホスファチジルエタノールアミン水解ホスホリパーゼD(NAPE-PLD)の活性化2007

    • Author(s)
      王 俊
    • Organizer
      第30回日本分子生物学会年会・第80回日本生化学会大会合同大会
    • Place of Presentation
      横浜市
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] N-アシルエタノールアミン水解酸性アミダーゼの免疫化学的解析2007

    • Author(s)
      趙 麗穎
    • Organizer
      第30回日本分子生物学会年会・第80回日本生化学会大会合同大会
    • Place of Presentation
      横浜市
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] Identification and characterization of a phosphatidylethanolamine N-acyltransferase generating the anandamide an endogenous marijuana-like substance2007

    • Author(s)
      Jin, X. H
    • Organizer
      The 48th Chugoku/Shikoku branch of the Japanese Biochemical Society
    • Place of Presentation
      Kochi
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] Identification and characterization of a novel phosphatidylethanolamine Nacyltransferase generating N-acylphosphatidylethanolamine2007

    • Author(s)
      Okamoto, Y
    • Organizer
      The 49th Japanese conference on the biochemistry of lipids
    • Place of Presentation
      Sapporo
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] Discovery of a calcium-independent phosphatidylethanolamine N-acyltransferase (iNAT)2007

    • Author(s)
      Ueda, N
    • Organizer
      International Cannabinoid Research Society 17th Annual Symposium on the Cannabinoids
    • Place of Presentation
      Saint-Sauveur, Quebec, Canada
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] Identification of the protein with a homology to lecithin-retinol acyltransferase as phosphatidylethanolamine N-acyltransferase2007

    • Author(s)
      Jin, X. H
    • Organizer
      BMB2007 (The 30st Annual Meeting of MBSJ & The 80st Annunl Meeting of JBS
    • Place of Presentation
      Yokohama
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] Activation of N-acylphoshatidyletanolamine-hydrolyzing phospholipase D (NAPE-PLD) by membrane lipids and phosphatidylethanolamine2007

    • Author(s)
      Wang, J
    • Organizer
      BMB2007 (The 30st Anual Meeting of MBSJ & The 80st Annunl Meeting of JBS
    • Place of Presentation
      Yokohama
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] Immunocheraical studies of N-acylethanolamine -hydrolyzing acid amidase2007

    • Author(s)
      Zhao, L. Y
    • Organizer
      BMB2007 (The 30st Annual Meeting of MBSJ & The 80st Annunl Meeting of JBS
    • Place of Presentation
      Yokohama
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] 内因性マリファナ様物質アナンダミドの生合成に係わる新規N一アシル転移酵素の同定と性状解析2007

    • Author(s)
      金星華
    • Organizer
      第48回日本生化学会中国・四国支部例会
    • Place of Presentation
      高知市
    • Related Report
      2007 Annual Research Report
  • [Presentation] Discovery of a calcium-independent phosphatidylethanolamin N-acyltransferase (iNAT)2007

    • Author(s)
      Ueda, N.
    • Organizer
      International Cannabinoid Research Society1 7th Annual Symposium on the Cannabinoids
    • Place of Presentation
      Saint-Sauveur, Quebec, Canada
    • Related Report
      2007 Annual Research Report
  • [Presentation] Immunochemical studies receal predominant expression of lysosomal N-acyleanolamine-hydrolyzing acid amidase in macrophages2007

    • Author(s)
      Tsuboi, K.
    • Organizer
      International Cannabinoid Research Society 17th Annual Symposium on the Cannabinoids
    • Place of Presentation
      Saint-Sauveur, Quebec, Canada
    • Related Report
      2007 Annual Research Report
  • [Presentation] 抗炎症脂質N-アシルエタノールアミンを加水分解する酸性アミダーゼのマクロファージにおける発現2007

    • Author(s)
      上田夏生
    • Organizer
      第28回臼本炎症・再生医学会
    • Place of Presentation
      東京都新宿区
    • Related Report
      2007 Annual Research Report
  • [Presentation] レシチン・レチノール・アシル転移酵素構造類似タンパク質のボスファチジルエタノールアミンN-'アシル転移酵素としての同定2007

    • Author(s)
      金星華
    • Organizer
      第30回日本分子生物学会年会・第80回日本生化学会大会合同大会
    • Place of Presentation
      横浜市
    • Related Report
      2007 Annual Research Report
  • [Presentation] 生体膜成分とリン脂質によるN-アシルボスファチジルエタノールアミン水解ボスホリパーゼD(NAPE-PLD)の活性化2007

    • Author(s)
      王 俊
    • Organizer
      第30回日本分子生物学会年会・第80回日本生化学会大会合同大会
    • Place of Presentation
      横浜市
    • Related Report
      2007 Annual Research Report
  • [Presentation] N-アシルエタノ-ルアミン水解酸性アミダ-ゼの免疫化学的解析2007

    • Author(s)
      趙 麗穎
    • Organizer
      第30回日本分子生物学会年会・第80回日本生化学会大会合同大会
    • Place of Presentation
      横浜市
    • Related Report
      2007 Annual Research Report
  • [Presentation] N-アシルホスファチジルエタノールアミンを特異的に加水分解するホスホリパーゼD(NAPE-PLD)の組換え酵素の性状解析・2006

    • Author(s)
      森下淳
    • Organizer
      第47回日本生化学会中国・四国支部例会
    • Place of Presentation
      松江市
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] Molecular biological studies on the anandamide-generating phospholipase D(NAPE-PLD)2006

    • Author(s)
      Ueda, N.
    • Organizer
      2nd International Conference on Nonmammalian Eicosanoids, Bioactive Lipids and Plant Oxylipins
    • Place of Presentation
      Berlin, Germany
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] アナンダミドを遊離する新規ホスホリパーゼDの組換え体の精製と性状解析2006

    • Author(s)
      岡本安雄
    • Organizer
      日本ビタミン学会第58回大会
    • Place of Presentation
      徳島市
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] アナンダミドを含むN-アシルエタノールアミンを遊離する新規ホスホリパーゼDの組換え酵素の大腸菌からの精製と性状解析2006

    • Author(s)
      岡本安雄
    • Organizer
      第48回日本脂質生化学会
    • Place of Presentation
      東京都江東区
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] Molecular. characterization of a novel lysosomal enzyme hydrolyzing anandamide and other bioactive N-acylethanolamines2006

    • Author(s)
      Tsuboi, K.
    • Organizer
      20th IUBMB International Congress of Biochemistry and Molecular Biology and 11th FAOBMB Congress
    • Place of Presentation
      Kyoto
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] Purification and characterization of the recombinant anandamide-generating phospholipase D2006

    • Author(s)
      Wang, J.
    • Organizer
      20th IUBMB International Congress of Biochemistry and Molecular Biology and 11th FAOBMB Congress
    • Place of Presentation
      Kyoto
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] Purification and characterization of recombinant NAPE-PLD expressed in Escherichiacoli2006

    • Author(s)
      Okamoto, Y.
    • Organizer
      International Cannabinoid Research Society 16th Annual Symposium on the Cannabinoids
    • Place of Presentation
      Tihany, Hungary
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] マクロファージにおける酸性アミダーゼによる抗炎症脂質N-アシルエタノールアミンの加水分解2006

    • Author(s)
      坪井一人
    • Organizer
      第27回日本炎症・再生医学会
    • Place of Presentation
      東京都新宿区
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] Expression analysis of N-acylethanolamine-generating phospholipase D in rat brain2006

    • Author(s)
      Morishita, J.
    • Organizer
      2006 International Symposium on Cell Signaling and Gene Regulation
    • Place of Presentation
      Tainan, Taiwan
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] Molecular. cloning and characterization of a novel lysosomal enzyme hydrolyzing anandamide and other bioactive N-acylethanolamines2006

    • Author(s)
      Tsuboi, K.
    • Organizer
      2006 International Symposium on Cell Signaling and Gene Regulation
    • Place of Presentation
      Tainan, Taiwan
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] Characterization of the recombinant N-acylphoshatidyletanolamine-hydrolyzing phospholipase D (NAPE-PLD)2006

    • Author(s)
      Morishita, J
    • Organizer
      The 47th Chugoku/Shikoku branch of the Japanese Biochemical Society
    • Place of Presentation
      Matsue
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] Molecular biological studies on the anandamide-generating phospholipase D (NAPE-PLD)2006

    • Author(s)
      Ueda, N
    • Organizer
      2nd International Conference on Nonmammalian Eicosanoids, Bioactive Lipids and Plant Oxylipins
    • Place of Presentation
      Berlin, Germany
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] Purification and characterization of the recombinant enzyme of a novel phospholipase D releasing anadamide2006

    • Author(s)
      Okamoto, Y
    • Organizer
      The 58th Annual Meeting of the Vitamin Society of Japan
    • Place of Presentation
      Tokushima
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] Purification and characterization of the recombinant enzyme of a novel phospholipase D releasing anadamide and other N-acylethanolamine from Escherichia coli2006

    • Author(s)
      Okamoto, Y
    • Organizer
      The 48th Japanese conference on the biochemistry of lipids
    • Place of Presentation
      Tokyo
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] Molecular.characterization of a novel lysosomal enzyme hydrolyzing anandamide and other bioactive Nacylethanolamines2006

    • Author(s)
      Tsuboi, K
    • Organizer
      20th IUBMB International Congress of Biochemistry and Molecular Biology and llth FAOBMB Congress
    • Place of Presentation
      Kyoto
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] Purification and characterization of the recombinant anandamide-generating phospholipase D2006

    • Author(s)
      Wang, J
    • Organizer
      20th IUBMB International Congress of Biochemistry and Molecular Biology and llth FAOBMB Congress
    • Place of Presentation
      Kyoto
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] Purification and characterization of recombinant NAPE-PLD expressed in Escherichia coli2006

    • Author(s)
      Okamoto, Y
    • Organizer
      International Cannabinoid Research Society 16th Annual Symposium on the Cannabinoids
    • Place of Presentation
      Tihany, Hungary
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] Molecular.cloning and characterization of a novel lysosomal enzyme hydrolyzing anandamide and other bioactive N-acylethanolamines2006

    • Author(s)
      Tsuboi, K
    • Organizer
      2006 International Symposium on Cell Signaling and Gene Regulation
    • Place of Presentation
      Tainan, Taiwan
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] Hydrolysis of anti-inflammatory lipid N-acylethanolamine by acid amidase in macrophages

    • Author(s)
      Tsuboi, K
    • Organizer
      The 27th Annual Meeting of the Japanese Society of Inflammation and Regeneration
    • Place of Presentation
      Tokyo
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] Expression of anti-inflammatory lipid N-acylethanolamine -hydrolyzing acid amidase in macrophages

    • Author(s)
      Tsuboi, K
    • Organizer
      The 28th Annual Meeting of the Japanese Society of Inflammation and Regeneration
    • Place of Presentation
      Tokyo
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary

URL: 

Published: 2006-04-01   Modified: 2016-04-21  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi