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Development of new gene therapy using gas plasma

Research Project

Project/Area Number 18590312
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Human genetics
Research InstitutionKyushu University

Principal Investigator

BABA Haruhisa  Kyushu University, 大学病院, 助教 (30419577)

Co-Investigator(Kenkyū-buntansha) TAKADA Hidetoshi  九州大学, 大学病院, 特任准教授 (70294931)
KATAYAMA Yoshiki  九州大学, 工学研究院, 教授 (70284528)
IHARA Kenji  九州大学, 大学病院, 講師 (80294932)
Project Period (FY) 2006 – 2008
Project Status Completed (Fiscal Year 2008)
Budget Amount *help
¥4,190,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥690,000)
Fiscal Year 2008: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2007: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2006: ¥1,200,000 (Direct Cost: ¥1,200,000)
Keywords遺伝子導入 / CD34陽性細胞 / プラズマ法
Research Abstract

我々はプラズマによる遺伝子導入法を開発した。これを用いて、癌細胞などの種々のcell line及びCD34陽性細胞への至的遺伝子導入法を検討した。遺伝子導入時に使用する培養液として、リン酸バッファー、MEM、RPMI、無血清培養液を比較検討した結果、リン酸バッファーと無血清培養液とが遺伝子導入効率が最も高いことが判明した。しかしリン酸バッファーを用いた場合、死細胞の割合が高かった。CD34陽性細胞への遺伝子導入効率は、プラズマ発生の条件や細胞培養液の条件を変えても、接着性細胞と比較して、著しく低かった。今後はCD34陽性細胞などの浮遊細胞での遺伝子導入効率を上げることが課題である。

Report

(4 results)
  • 2008 Annual Research Report   Final Research Report ( PDF )
  • 2007 Annual Research Report
  • 2006 Annual Research Report
  • Research Products

    (58 results)

All 2009 2008 2007 2006 Other

All Journal Article (45 results) (of which Peer Reviewed: 6 results) Presentation (13 results)

  • [Journal Article] FBP17 Mediates a Common Molecular Step in the Formation of Podosomes and Phagocytic Cups in Macrophages2009

    • Author(s)
      Tsuboi S, Takada H, Hara T, Mochizuki N, Funyu T, Saitoh H, Terayama Y, Yamaya K, Ohyama C, Nonoyama S, Ochs HD.
    • Journal Title

      J Biol Chem 284

      Pages: 8548-56

    • Related Report
      2008 Final Research Report
  • [Journal Article] Cardiovascular Complications Associated with Chronic Active Epstein-Barr Virus Infection2009

    • Author(s)
      Muneuchi J, Ohga S, Ishimura M, Ikeda K, Yamaguchi K, Nomura A, Takada H, Abe Y, Hara T.
    • Journal Title

      Pediatr Cardiol. (Epub ahead of print)

    • Related Report
      2008 Final Research Report
  • [Journal Article] FBP17 mediates a common molecular step in the formation of podosomes and phagocytic cups in macrophages.2009

    • Author(s)
      Tsuboi s, Takada H, et al.
    • Journal Title

      Journal of Biological Chemistry 284

      Pages: 8548-56

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Lipopolysaccharide-induced monocytic cell death for the diagnosis of mild neonatal-onset multisystem inflammatory disease2008

    • Author(s)
      Takada H, Ishimura M, Inada H, Ohga S, Kusuhara K, Moroi Y, Furue M, Hara T.
    • Journal Title

      J Pediatr 152

      Pages: 885-7

    • Related Report
      2008 Final Research Report
  • [Journal Article] Epstein-Barr virus load in cerebrospinal fluid of patients with chronic active Epstein-Barr virus infection2008

    • Author(s)
      Ohga S, Sanefuji M, Ishimura M, Nomura A, Torisu H, Kira R, Takada H, Mizuno Y, Kazuyama Y, Hara T.
    • Journal Title

      Pediatr Infect Dis J. 27

      Pages: 1027-30

    • Related Report
      2008 Final Research Report
  • [Journal Article] Sjogren's syndrome-associated meningoencephalomyelitis : cerebrospinal fluid cytokine levels and therapeutic utility of tacrolimus2008

    • Author(s)
      Hoshina T, Yamaguchi Y, Ohga S, Kira R,Ishimura M, Takada H, Tanaka T, Hara T.
    • Journal Title

      J Neurol Sci 267

      Pages: 182-6

    • Related Report
      2008 Final Research Report
  • [Journal Article] Thromboembolic complications in splenectomized patients with dominantly inherited beta-thalassemia2008

    • Author(s)
      Ohga S, Ideguchi H, Kato J, Ishimura M,Takada H, Harada N, Kawanaka H, Hattori Y, Kang D, Hamasaki N, Hara T.
    • Journal Title

      Acta Haematol 120

      Pages: 31-5

    • Related Report
      2008 Final Research Report
  • [Journal Article] Epstein-Barr virus-associated meningoencephalomyelitis : intrathecal reactivation of the virus in an immunocompetent child2008

    • Author(s)
      Sanefuji M, Ohga S, Kira R, Nomura A,Torisu H, Takada H, Kusuhara K, Hara T.
    • Journal Title

      J Child Neurol 23

      Pages: 1072-7

    • Related Report
      2008 Final Research Report
  • [Journal Article] 血球貪食症候群の発症機序2008

    • Author(s)
      高田英俊、大賀正一、原寿郎
    • Journal Title

      サイトカイン血液・腫瘍科 57[suppl.6]

      Pages: 40-5

    • Related Report
      2008 Final Research Report
  • [Journal Article] EBウイルスに伴う血球貪食症候群2008

    • Author(s)
      大賀正一、石村匡崇、菅尚浩、野村明彦、高田英俊、原寿郎
    • Journal Title

      発症機序血液・腫瘍科 57[Suppl. 6]

      Pages: 116-21

    • Related Report
      2008 Final Research Report
  • [Journal Article] 自然免疫による免疫不全症2008

    • Author(s)
      高田英俊、原寿郎
    • Journal Title

      小児科臨床 61

      Pages: 1783-9

    • Related Report
      2008 Final Research Report
  • [Journal Article] インターフェロンガンマ受容体異常症と類似疾患2008

    • Author(s)
      高田英俊、原寿郎
    • Journal Title

      小児内科40増刊号小児疾患診療のための病態生理 1

      Pages: 1356-9

    • Related Report
      2008 Final Research Report
  • [Journal Article] Disseminated Bacillus Calmette-Guerin lymphadenitis in a patient with gp91phox(-)chronic granulomatous disease 25 years after vaccination2008

    • Author(s)
      Kusuhara K, Ohga S, Hoshina T, Saito M,Sasaki Y, Ishimura M, Takada H, Fujita M, Hara T.
    • Journal Title

      Eur J Pediatr (Epub ahead of print)

    • Related Report
      2008 Final Research Report
  • [Journal Article] Fulminant sepsis/meningitis due to Haemophilus influenzae in a protein C-deficient heterozygote treated with activated protein C therapy. Eur J Pediatr2008

    • Author(s)
      Ishimura M, Saito M, Ohga S, Hoshina T,Baba H, Urata M, Kira R, Takada H, Kusuhara K, Kang D, Hara T.
    • Journal Title

      (Epub ahead of print)

    • Related Report
      2008 Final Research Report
  • [Journal Article] Lack of association between E148Q MEFV variant and Kawasaki disease2008

    • Author(s)
      Yamaguchi K, Ikeda K, Ihara K, Takada H, Kusuhara K, Hara T.
    • Journal Title

      Hum Immunol Nov19(Epub ahead of print)

    • Related Report
      2008 Final Research Report
  • [Journal Article] Complement component 9 deficiency is not associated with the development of gastric carcinoma and leukaemia2008

    • Author(s)
      Kuroki-Nagamatsu R, Koga Y, Suminoe A,Matsuzaki A, Ihara K, Oki E, Kakeji Y, Maehara Y, Hara T
    • Journal Title

      Molecular Immunology 45

      Pages: 4176-4176

    • Related Report
      2008 Final Research Report
  • [Journal Article] Association study between B- and T-lymphocyte attenuator gene and type 1 diabetes mellitus or systemic lupus erythematosus in the Japanese population2008

    • Author(s)
      Inuo M, Ihara K, Matsuo T, Kohno H, Hara T
    • Journal Title

      Int J Immunogenet (in press)

    • Related Report
      2008 Final Research Report
  • [Journal Article] Genetic analysis of MMP gene polymorphisms in patients with Kawasaki disease2008

    • Author(s)
      Ikeda K, Ihara K, Yamaguchi K, Muneuchi J, Ohno T, Mizuno Y, Hara T
    • Journal Title

      Pediatr Res 63(2)

      Pages: 182-5

    • Related Report
      2008 Final Research Report
  • [Journal Article] Lipopolysaccharide-induced monocytic cell death for the diagnosis of mild neonatal-onset multisystem inflammatory disease2008

    • Author(s)
      Takada H, Ishimura M, et al.
    • Journal Title

      Journal of Pediatrics 152

      Pages: 885-887

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Lipopolysaccharide-induced monocytic cell death for the diagnosis of mild neonatal-onset multisystem inflammatory disease2008

    • Author(s)
      Takada H, Ishimura M, et. al.
    • Journal Title

      Journal of Pediatrics (印刷中)

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Journal Article] PD-1 gene haplotype is associated with the development of type 1 diabetes mellitus in Japanese children2007

    • Author(s)
      Ni R, Ihara K, Miyako K, Kuromaru R,Inuo M, Kohno H, Hara T
    • Journal Title

      Hum Genet 121(2)

      Pages: 223-32

    • Related Report
      2008 Final Research Report
  • [Journal Article] The Leu544Ile Polymorphism of the growth hormone receptor gene affects the serum cholesterol levels during GH deficiency2007

    • Author(s)
      Ihara K, Inuo M, Kuromaru R, Miyako K,Kohno H, Kinukawa N, Hara T
    • Journal Title

      Clin Endocrinol 67

      Pages: 212-217

    • Related Report
      2008 Final Research Report
  • [Journal Article] Novel mutations in five Japanese patients with 3-methylcrotonyl-CoA carboxylase deficiency2007

    • Author(s)
      Uematsu M, Sakamoto O, Sugawara N,Kumagai N, Morimoto T, Yamaguchi S,Hasegawa Y, Kobayashi H, Ihara K,Yoshino M, Watanabe Y, Inokuchi T,Yokoyama T, Kiwaki K, Nakamura K, EndoF, Tsuchiya S, Ohura T
    • Journal Title

      J Hum Genet 52(12)

      Pages: 1040-3

    • NAID

      10020022418

    • Related Report
      2008 Final Research Report
  • [Journal Article] Tissue inhibitor of metalloproteinase 2 and coronary artery lesions in Kawasaki disease2007

    • Author(s)
      Furuno K, Takada H, Yamamoto K, Ikeda K, Ohno T, Khajoee V, Mizuno Y, Hara T.
    • Journal Title

      J Pediatr 151

      Pages: 155-60

    • Related Report
      2008 Final Research Report
  • [Journal Article] Dominant-negative mutations in the DNA-binding domain of STAT3 cause hyper-IgE syndrome2007

    • Author(s)
      Minegishi Y, Saito M, Tsuchiya S, Tsuge I, Takada H, Hara T, Kawamura N, Ariga T, Pasic S, Stojkovic O, Metin A,Karasuyama H
    • Journal Title

      Nature 448

      Pages: 1058-62

    • Related Report
      2008 Final Research Report
  • [Journal Article] Selective predisposition to bacterial infections in IRAK-4-deficient children : IRAK-4-dependent TLRs are otherwise redundant in protective immunity2007

    • Author(s)
      Ku CL, von Bernuth H, Picard C, Zhang SY, Chang HH, Yang K, Chrabieh M,Issekutz AC, Cunningham CK, Gallin J, Holland SM, Roifman C, Ehl S, Smart J, Tang M, Barrat FJ, Levy O, McDonald D, Day-Good NK, Miller R, Takada H, Hara T, Al-Hajjar S, Al-Ghonaium A, Speert D, Sanlaville D, Li X, Geissmann F, Vivier E, Marodi L, Garty BZ, Chapel H, Rodriguez-Gallego C, Bossuyt X, Abel L, Puel A, Casanova JL.
    • Journal Title

      J Exp Med 204

      Pages: 2407-22

    • Related Report
      2008 Final Research Report
  • [Journal Article] 本邦初のInterleukin-1 receptor associated kinase 4欠損症兄弟例の臨床的特徴2007

    • Author(s)
      吉川秀人、北村太郎、渡辺周永、虻川大樹、稲垣徹史、武山淳二、今泉益栄、高田英俊、原 寿郎
    • Journal Title

      日本小児科学会雑誌 111

      Pages: 750-4

    • NAID

      10019547650

    • Related Report
      2008 Final Research Report
  • [Journal Article] Successful self-infusion of activated prothrombin complex concentrate for prophylaxis in a child with a factor VIII inhibitor2007

    • Author(s)
      Ohga S, Nomura A, Takada H, Suga N, Hara T.
    • Journal Title

      Am J Hematol 82

      Pages: 145-9

    • Related Report
      2008 Final Research Report
  • [Journal Article] Early-onset sarcoidosis mimicking refractory cutaneous histiocytosis2007

    • Author(s)
      Ohga S, Ichino K, Urabe K, Ishimura M,Takada H, Nishikomori R, Furue M, Hara T.
    • Journal Title

      Pediatr Blood Cancer 50

      Pages: 723-6

    • Related Report
      2008 Final Research Report
  • [Journal Article] Malignant hepatic tumor occurring2007

    • Author(s)
      Ishimura M, Ohga S, Nagatoshi Y,Okamura J, Tajiri T, Kohashi K, Oda Y,Takada H, Hara T.
    • Journal Title

      10 yrs after a histocompatible sibling donor bone marrow transplantation for severe aplastic anemia. Pediatr Transplant 11

      Pages: 945-9

    • Related Report
      2008 Final Research Report
  • [Journal Article] 結核菌/非定型抗酸菌に対する易感染性とIL-12/IFN-・経路2007

    • Author(s)
      楠原浩一、高田英俊、原寿郎
    • Journal Title

      日本サルコイドーシス/肉芽腫性疾患学会雑誌 27

      Pages: 9-19

    • Related Report
      2008 Final Research Report
  • [Journal Article] 血球貪食症候群2007

    • Author(s)
      大賀正一、野村明彦、高田英俊、原寿郎
    • Journal Title

      小児内科 39

      Pages: 2078-81

    • Related Report
      2008 Final Research Report
  • [Journal Article] 慢性活動性EBウイルス感染症-病態と治療-2007

    • Author(s)
      大賀正一、石村匡崇、野村明彦、高田英俊、原寿郎
    • Journal Title

      日本小児皮膚科学会雑誌 26

      Pages: 69-73

    • NAID

      10019963951

    • Related Report
      2008 Final Research Report
  • [Journal Article] 自己炎症性症候群2007

    • Author(s)
      原寿郎、高田英俊、楠原浩一
    • Journal Title

      小児科臨床 60

      Pages: 1505-16

    • NAID

      10018652826

    • Related Report
      2008 Final Research Report
  • [Journal Article] Dominant-negative mutations in the DNA-binding domain of STAT3 cause hyper-IgE syndrome2007

    • Author(s)
      Minegishi Y, -, Takada H, et. al.
    • Journal Title

      Nature 448

      Pages: 1058-1062

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Selective predisposition to bacterial infections in IRAK-4-deficient children:IRAK-4-dpendent TLRs are otherwise redundant protective immunity2007

    • Author(s)
      Ku CL, -, Takada, et. al.
    • Journal Title

      Journal of Experimental Medicine 204

      Pages: 2407-2422

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Association study of polymorphisms in SOCS family genes with type 1 diabetes mellitus2006

    • Author(s)
      Ni R, Ihara K, Miyako K, Takemoto M, Ishimura M, Kohno H, Matsuura N, Yoshimura A, Hara T
    • Journal Title

      Int J Immunogenet 33(1)

      Pages: 7-10

    • Related Report
      2008 Final Research Report
  • [Journal Article] Phenotypic and genetic analyses of T-cell-mediated immunoregulation in patients with Type 1 diabetes2006

    • Author(s)
      Tsutsumi Y, Jie X, Ihara K, Nomura A,Kanemitsu S, Takada H, Hara T
    • Journal Title

      Diabet Med 23(10)

      Pages: 1145-50

    • Related Report
      2008 Final Research Report
  • [Journal Article] Human tyrosine kinase 2 deficiency reveals its requisite roles in multiple cytokine signals involved in innate and acquired immunity.2006

    • Author(s)
      Minegishi Y, Takada H (7番目)ら
    • Journal Title

      Immunity 25・5

      Pages: 745-55

    • Related Report
      2006 Annual Research Report
  • [Journal Article] Phenotypic and genetic analysis of T-cell-mediated immunoregulation in patients with type 1 diabetes.2006

    • Author(s)
      Tsutsumi Y, Ihara K (3番目), Takada H (6番目)ら
    • Journal Title

      Diabet Med 23・10

      Pages: 1145-50

    • Related Report
      2006 Annual Research Report
  • [Journal Article] PD-1 gene haplotype is associated with the development of type 1 diabetes mellitus in Japanese children.2006

    • Author(s)
      Ni R, Ihara K ら
    • Journal Title

      Hum genet 26・5

      Pages: 417-20

    • Related Report
      2006 Annual Research Report
  • [Journal Article] Reduced gene expression of clustered ribosomal proteins in Diamond-Blackfan anemia patients without RPS19 gene mutations.2006

    • Author(s)
      Kaga Y, Takada H (4番目)ら
    • Journal Title

      J Pediatr Hematol Oncol 28・6

      Pages: 355-61

    • Related Report
      2006 Annual Research Report
  • [Journal Article] Delayed separation of the umbilical cord in two siblings with interleukin-1 receptor-associated kinase 4 deficiency.2006

    • Author(s)
      Takada H ら
    • Journal Title

      J Pediatr 148・4

      Pages: 546-8

    • Related Report
      2006 Annual Research Report
  • [Journal Article] Association study of polymorphisms in SOCS family genes with type 1 diabetes mellitus2006

    • Author(s)
      Ni R, Ihara K ら
    • Journal Title

      Int J Immunogenet 33・1

      Pages: 7-10

    • Related Report
      2006 Annual Research Report
  • [Journal Article] Lack of association between E148Q MEFV variant and Kawasaki disease.

    • Author(s)
      Yamaguchi K, ---Takada H, et al.
    • Journal Title

      Human Immunology (印刷中)

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Presentation] Novel primary immunodeficiency syndrome with a developmental defect of pro-Bcells, NK cells and plasmacytoid dendritic cells2008

    • Author(s)
      Takada H
    • Organizer
      第38回日本免疫学会
    • Place of Presentation
      京都
    • Year and Date
      2008-12-02
    • Related Report
      2008 Annual Research Report
  • [Presentation] NEMO mutation as a cause of familial occurrence of female Behcet's disease patisnts2008

    • Author(s)
      Hara T, Takada H
    • Organizer
      XX International Congress of Genetics
    • Place of Presentation
      Berlin, Germany
    • Related Report
      2008 Final Research Report
  • [Presentation] Pathophysiology and treatment of Diamond-Blackfan anemia SYMPOSIUM 4 Hematological disorders in mature and premature infants by JSPS-NRCT program and the 18th Annual Meeting of the Japan Society of Obstetrical2008

    • Author(s)
      Ohga S, Koga Y, Ishimura M, Takada H, Hara T
    • Organizer
      Gynecological and Neonatal Hematology
    • Place of Presentation
      Fukuoka
    • Related Report
      2008 Final Research Report
  • [Presentation] Toshiro Hara : Novel primary immunodeficiency syndrome with a developmental defect of pro-B cells2008

    • Author(s)
      Hidetoshi Takada, Yojiro Arinobu,Masataka Ishimura, Koichi Akashi
    • Organizer
      NK cells and plasmacytoid dendritic cells第38回日本免疫学会
    • Place of Presentation
      京都
    • Related Report
      2008 Final Research Report
  • [Presentation] ISHIKAWA Fumihiko : Development of functional human B cells in NOD/SCID/IL2r・KO mice and the application of the xenotransplant model in studying human immunodeficiency2008

    • Author(s)
      Takehiko Doi, TAKADA Hidetoshi,KANEGANE Hirokazu, TOMIZAWA Mariko,NAKAYAMA Toshinori, OHARA Osamu, MIYAWAKI Toshio, HARA Toshiro
    • Organizer
      第38回日本免疫学会総会・学術集会
    • Place of Presentation
      京都
    • Related Report
      2008 Final Research Report
  • [Presentation] NEMO mutation and Behcet disease2007

    • Author(s)
      高田 英俊, ら
    • Organizer
      日本免疫学会
    • Place of Presentation
      大阪
    • Year and Date
      2007-10-19
    • Related Report
      2007 Annual Research Report
  • [Presentation] Kobayashi M, Miyawaki T: Genetic backgrounds of patients with susceptibility to mycobacterial infections in Japan2007

    • Author(s)
      Hara T, Takada H, Hoshina T, Sasaki Y, Saito M, Nomura A, Kusuhara K,
    • Organizer
      25th International Congress of Pediatrics
    • Place of Presentation
      Athens, Greece
    • Related Report
      2008 Final Research Report
  • [Presentation] Malignant hepatic tumor occurring 10 years after a histocompatible sibling donor bone marrow transplantation for severe aplastic anemia 14th AnnualMeeting of the Japan Childhood2007

    • Author(s)
      Ishimura M, Ohga S, Nagatoshi Y, Okamura J, Oda Y, Takada H, Hara T
    • Organizer
      Aplastic Anemia Study Group
    • Place of Presentation
      Nagoya, Japan
    • Related Report
      2008 Final Research Report
  • [Presentation] Congenital pure red cell aplasia as a human ribosomopathy SESSION I2007

    • Author(s)
      Ohga S, Koga Y, Ishimura M, Nomura A,Takada H, Hara T
    • Organizer
      Prenatal and Pediatric Research for Future Development of Cure, Care and Prevention of Congenital Disease The 18th Fukuoka International Symposium on Pediatric/Maternal-Child Health Research September 1st
    • Place of Presentation
      Fukuoka,Japan
    • Related Report
      2008 Final Research Report
  • [Presentation] Central nervous system reactivation of Epstein-Barr virus(EBV)in chronic active EBV infection and EBV-associated encephalitis The 3rd Congress of Asian Society for Pediatric Research2007

    • Author(s)
      Ohga S, Sanefuji M, Ishimura M, Nomura A, Torisu H, Kira R, Takada H, Mizuno Y, Kazuyama Y, Hara T
    • Place of Presentation
      Tokyo, Japan
    • Related Report
      2008 Final Research Report
  • [Presentation] Clinical outcomes of cardiac complications associated with chronic active Epstein-Barr virus infection(CAEBV)2007

    • Author(s)
      Muneuchi J, Ohga S, Ishimura M,Yamawaki K, Kanaya Y, Takada H, Hara T
    • Organizer
      The 3rd Congress of Asian Society for Pediatric Research(Oral)
    • Place of Presentation
      Tokyo, Japan
    • Related Report
      2008 Final Research Report
  • [Presentation] Malignant hepatic tumor occurring 10 years after a histocompatible sibling donor bone marrow transplantation for severe aplastic anemia2007

    • Author(s)
      Ishimura M, Ohga S, Nagatoshi Y,Okamura J, Tajiri T, Kohashi K, Oda Y,Takada H, Hara T
    • Organizer
      The 3rd Congress of Asian Society for Pediatric Research
    • Place of Presentation
      Tokyo, Japan
    • Related Report
      2008 Final Research Report
  • [Presentation] IRAK4 deficiency in Japan Symposium for PID in ASIA

    • Author(s)
      Takada H
    • Place of Presentation
      Yokohama
    • Related Report
      2008 Final Research Report

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Published: 2006-04-01   Modified: 2016-04-21  

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