Development of In Situ Methylation-Specific PCR
| Project/Area Number |
18590330
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Human pathology
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| Research Institution | Yamaguchi University |
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Principal Investigator |
KAWAUCHI Shigeto Yamaguchi University, Graduate School of Medicine, Associate Professor (80284511)
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| Co-Investigator(Kenkyū-buntansha) |
SASAKI Kohsuke Yamaguchi University, Graduate School of Medicine, Professor (80116722)
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| Project Period (FY) |
2006 – 2007
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| Project Status |
Completed (Fiscal Year 2007)
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| Budget Amount *help |
¥2,070,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥270,000)
Fiscal Year 2007: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2006: ¥900,000 (Direct Cost: ¥900,000)
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| Keywords | in situ MSP / epigenetics / CpG island cytosine hypermethylation / in situ PCR / methylatio-specific PCR / gene silencing / 遺伝子CpGメチル化 |
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| Research Abstract |
Gene promoter hypermethylation is one of the essential mechanisms that regulate gene expression and silencing and associated with imprinting of gene, carcinogenesis and other various problems concerning clinical medicine. Our aim is to develop a method visualizing cells with p16 gene promoter CpG islands hypermethylation on a tissue section, ie, in situ methylation-specific PCR (in situ MSP). The method consists of three steps; 1^<st>, protease digestion and microwave irradiation to skeletonize genomic DNA on a tissue section; 2^<nd>, cytosine to uracil transition by sodium bisulfite; 3^<rd>, amplification and visualization by in situ PCR technique. In the present study, we established a prototype technique for in situ MSP and presented it in the 97^<th> annual meeting of the Japanese Society of Pathology. Paper is now on preraration.
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Report
(3 results)
Research Products
(16 results)
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[Journal Article] Influence of cytological stains on comparative genomic hybridization analysis for DNA extracted from cytological smears2007
Author(s)
Kawauchi, S, Sugimoto S, Liu, XP, Ikemoto K, Furuya, T, Takahashi, M, Sasaki, K
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Journal Title
Oncology Reports 18-5
Pages: 1219-1223
Description
「研究成果報告書概要(欧文)」より
Related Report
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[Journal Article] Chromosomal aberrations detected by comparative genomic hybridization predict outcome in patients with colorectal carcinoma2007
Author(s)
Liu, XP, Kawauchi, S, Oga, A, Sato, T, Ikemoto, K, Ikeda, E, Sasaki, K
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Journal Title
Oncology Reports 17
Pages: 261-267
Description
「研究成果報告書概要(欧文)」より
Related Report
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[Journal Article] Significance of cyclin E and p27 expression in malignant ovarian germ cell tumors : correlation with the cell proliferation activity and clinicopathologic features2006
Author(s)
Kawauchi, S, Yamamoto, Y, Uchida, K, Chochi, Y, Kondo T, Oga, A, Sasaki, K
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Journal Title
Oncology Reports 16-5
Pages: 1029-1033
Description
「研究成果報告書概要(欧文)」より
Related Report
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[Presentation] In situ methylation-specific polymerase chain reaction for detecting cells bearing p16 gene promoter CpG islands hypermethylation on a tissue section2007
Author(s)
Kawauchi, S, Suehiro, Y, Chochi, Y, Kondo, T, Oga, A, Sasaki, K
Organizer
97th Japanese Society of Pathology
Place of Presentation
Kanazawa, JPN.
Description
「研究成果報告書概要(欧文)」より
Related Report
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[Book] 子宮腫瘍病理アトラス2007
Author(s)
本山 悌一, 他
Total Pages
325
Publisher
文光堂
Description
「研究成果報告書概要(和文)」より
Related Report
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