• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

New method for nucleic acid amplification on pathologic tissue section of malignant lymphoma using LAMP method

Research Project

Project/Area Number 18590341
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Human pathology
Research InstitutionNara Medical University

Principal Investigator

NAKAMINE Hirokazu  Nara Medical University, Dept Med, Assoc. Prof. (70155810)

Co-Investigator(Kenkyū-buntansha) TAKAHIKO Kasai  Nara Medical University, Dept Med, Instructor (50224374)
ENOMOTO Yasunori  Nara Medical University, Dept Med, Instructor (60336849)
TAKEDA Maiko  Nara Medical University, Dept Med, Instructor (40398441)
Project Period (FY) 2006 – 2007
Project Status Completed (Fiscal Year 2007)
Budget Amount *help
¥2,610,000 (Direct Cost: ¥2,400,000、Indirect Cost: ¥210,000)
Fiscal Year 2007: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2006: ¥1,700,000 (Direct Cost: ¥1,700,000)
KeywordsMalignant lymphoma / LAMP method / in situ nucleic acid amplification / Follicular lymphoma / bcl-2 rearrangement / FISH method / in situ 核酸増幅 / bc1-2再構成
Research Abstract

Classification of malignant lymphoma is important for selection of treatment modality as well as for investigation of pathogenesis. Because B-cell lymphomas consist of several types which are correspond well to chromosomal/genetic abnormalities, more accurate classification can be performed when these abnormalities are detectable at daily pathology practice. However ; it is rather general in Japan that the first biopsy for pathological diagnosis is often performed at the regional hospitals rather than at the specialized medical institutions. The patients are transferred to the latter institutions where the second biopsy is sometimes required for examination of chromosomal/genetic abnormalities. This is due to the limitation and difficulty in performing such examination on paraffin-embedded tissue. To overcome this problem, we attempted to establish a new method for microscopic visualization of these abnormalities by amplification of genes involved in them on microscopic glass slides. We employed loop-mediated isothermal amplification (LAMP) instead of conventional PCR for this in situ DNA amplification, because LAMP method has several advantages over the conventional PCR for this purpose, including lower degree of tissue damages by using lower temperature, use of small-sized DNA polymerase, and larger size of amplification products. We fried to in situ amplify the rearranged bcl-2/IgH genes as a result of t (14 ; 18) which are present in more than 80% cases of follicular lymphoma. However, this attempt appears to be more difficult than initially thought and satisfactory amplification has not been obtained so far In the literature, only a single report of successful detection of point mutation of some gene by this in situ LAMP (Ikeda, et. al. Pathol Int 57, 594-599, 2007), indicating that there may be unexpected problems. Through this trial, however ; several minor findings regarding B-cell lymphoma could be obtained.

Report

(3 results)
  • 2007 Annual Research Report   Final Research Report Summary
  • 2006 Annual Research Report
  • Research Products

    (32 results)

All 2008 2007 2006

All Journal Article (20 results) (of which Peer Reviewed: 8 results) Presentation (11 results) Book (1 results)

  • [Journal Article] The t(1;3)(p^<34>;q^<27>)translocation A nonrandom BCL6 reamangement in diffuse large B cell lymphoma2008

    • Author(s)
      Niitsu N
    • Journal Title

      Ann Hematol 87

      Pages: 151-153

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Primary histiocytic sarcoma of the spleen associated with hemophagocytosis.2008

    • Author(s)
      Oka K
    • Journal Title

      Int J Hematol (Epub)

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Journal Article] B細胞リンパ腫病理診断の基本.びまん性大細胞型B細胞リンパ腫を中心に2007

    • Author(s)
      中峯 寛和
    • Journal Title

      臨床血液 48

      Pages: 107-116

    • NAID

      10018733270

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Annual Research Report 2007 Final Research Report Summary
  • [Journal Article] Clinicopathologic correlations of stage IE/IIE primary thyroid difuselarge B-cell lymphoma2007

    • Author(s)
      Niitsu N
    • Journal Title

      Ann Oncol 18

      Pages: 1203-1208

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
    • Peer Reviewed
  • [Journal Article] Prognostic impact of chromosomal alteration of 3q27 on nodal B-cell lymphoma. Correlation with histology, immunophenotype, karyotype, and clinical outcome in 329 consecutive patients.2007

    • Author(s)
      Niitsu N, et. al.
    • Journal Title

      Leuk Res 2007 31(9)(peer reviewed)

      Pages: 1191-1197

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] Primary hepatic low-grade B-cell lymphoma of MALT-type associated with Helicobacter pylori infection.2007

    • Author(s)
      Iida T, et. al.
    • Journal Title

      Hepatogastroenterology 54(peer reviewed)

      Pages: 1898-1901

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] Basic pathology diagnostic for B-cell lymphoma prilmaliry focusing on diffuse large B-cell lymphoma.2007

    • Author(s)
      Nakamine H, et. al.
    • Journal Title

      Jpn J Clin Hematol 48(2)(invited article)

      Pages: 107-116

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] 近い将来,私たちが遭遇する機会が増すと思われる疾患:(1)メトトレキセート(MTX)関連リンパ増殖疾患2007

    • Author(s)
      中峯 寛和
    • Journal Title

      病理と臨床 25

      Pages: 251-257

    • Related Report
      2007 Annual Research Report
  • [Journal Article] Prognostic impact of chromosomal alteration of 3q27 on nodal B-cell lymphoma Correlation with histology,immunophenotype,karyotype,and clinical outcome in 329 consecutive patients2007

    • Author(s)
      Niitsu N
    • Journal Title

      Leuk Res 31

      Pages: 1191-1197

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Clinicopathologic correlations of stage IE/IIE primary thyroid diffuse large B-cell lymphoma2007

    • Author(s)
      Niitsu N
    • Journal Title

      Ann Oncol 18

      Pages: 1203-1208

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Journal Article] t(6;14)(q15;q^<32>)in a patient with CD5+CD10+diffuse large B-cell lymphoma2007

    • Author(s)
      Hayama M
    • Journal Title

      Int J Hematol 86

      Pages: 147-149

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Journal Article] 脾原発(真の)組織球肉腫の診断と鑑別診断2007

    • Author(s)
      中峯 寛和
    • Journal Title

      病理と臨床 25

      Pages: 486-487

    • Related Report
      2007 Annual Research Report
  • [Journal Article] Primary hepatic low-grade B-cell lymphoma of MALT-type associated with Helico bacter pylori infection2007

    • Author(s)
      Iida T
    • Journal Title

      Hepatogastroenterology 54

      Pages: 1898-1901

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Journal Article] リンパ節腫大を初発症状としたLangerhans細胞肉腫.診断に至ったプロセス2007

    • Author(s)
      中峯 寛和
    • Journal Title

      病理と臨床 25

      Pages: 176-177

    • Related Report
      2007 Annual Research Report
  • [Journal Article] Follicular lymphoma frequently originates in the salivary gland2006

    • Author(s)
      Nakamura S
    • Journal Title

      Pathol Int 56

      Pages: 576-583

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary 2006 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Follicular lymphoma frequently originates in the salivary gland2006

    • Author(s)
      Nakamura S, et. al.
    • Journal Title

      Pathol Int 56(10)(peer reviewed)

      Pages: 576-583

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] t(8;14)(q24;q32) in two patients with CD10-negative primary thyroid diffuse large B-cell lymphoma2006

    • Author(s)
      Niitsu N
    • Journal Title

      Leuk Res Epub

    • Related Report
      2006 Annual Research Report
  • [Journal Article] Recurrent primary cutaneous large B-cell lymphoma after a long-term remission2006

    • Author(s)
      Yamamoto Y
    • Journal Title

      Pathol Int 56

      Pages: 645-647

    • Related Report
      2006 Annual Research Report
  • [Journal Article] 精巣腫大にて発症し、皮膚浸潤をきたしたNK/T cell lymphomaの1例2006

    • Author(s)
      北村華奈
    • Journal Title

      臨床皮膚科 60

      Pages: 725-728

    • Related Report
      2006 Annual Research Report
  • [Journal Article] Prognostic impact of chromosomal alteration of 3q27 on nodal B-cell lymphoma. Correlation with histology, immunophenotype, karyotype, and clinical outcome in 329 consecutive patients2006

    • Author(s)
      Niitsu N
    • Journal Title

      Leuk Res Epub

    • Related Report
      2006 Annual Research Report
  • [Presentation] t(14;18)(q32;q21)を有するびまん性大細胞型B細胞リンパ腫の付加的染色体異常に関する検討2007

    • Author(s)
      岡本 昌隆
    • Organizer
      第49回日本臨床血液学会総会
    • Place of Presentation
      横浜市
    • Year and Date
      2007-10-12
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Annual Research Report 2007 Final Research Report Summary
  • [Presentation] Analysis of additional chromosomal abnormalities seen in diffuse large B-cell lymphomas having t(14 ; 18)(q32 ; q21).2007

    • Author(s)
      Okamoto M, et. al.
    • Organizer
      Jpn Soc Clin Hematol, 49th annual meeting
    • Year and Date
      2007-10-12
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] Diffuse large B-cell lymphoma progressed from plasma cell-like low grade B-cell lymphoma.2007

    • Author(s)
      Ishii T, et. al.
    • Organizer
      Jpn Soc Clin Hematol, 49th annual meeting
    • Year and Date
      2007-10-12
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] びまん性大細胞型B細胞リンパ腫に急速に進展した.形質細胞類似の低悪性度B細胞腫瘍2007

    • Author(s)
      石井 隆司
    • Organizer
      第49回日本臨床血液学会総会
    • Place of Presentation
      横浜市
    • Year and Date
      2007-10-11
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] 肺門リンパ節腫脹の長期経過観察中に発症したhistiocytic sarcomaの1例2007

    • Author(s)
      小野 香奈子
    • Organizer
      第49回日本臨床血液学会総会
    • Place of Presentation
      横浜市
    • Year and Date
      2007-10-11
    • Related Report
      2007 Annual Research Report
  • [Presentation] びまん性大細胞型B細胞リンパ腫に急速に進展した形質細胞類似の低悪性度B細胞腫瘍2007

    • Author(s)
      石井 隆司
    • Organizer
      第49回日本臨床血液学会総会
    • Place of Presentation
      横浜市
    • Year and Date
      2007-10-11
    • Related Report
      2007 Annual Research Report
  • [Presentation] 末梢性T細胞リンパ腫再発時にT/B両クローンが検出されEBV陽性細胞の増殖を認めた1例2007

    • Author(s)
      沢田 仁
    • Organizer
      第47回日本リンパ網内系学会総会.
    • Place of Presentation
      淡路市
    • Year and Date
      2007-05-27
    • Related Report
      2007 Annual Research Report
  • [Presentation] メソトレキセート(MTX)関連リンパ球増殖性疾患(LPD)の診断2007

    • Author(s)
      中峯 寛和
    • Organizer
      第96回日本病理学会総会
    • Place of Presentation
      大阪市
    • Year and Date
      2007-03-14
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] 末梢血中に形質細胞様リンパ球増多を認めたEBV関連リンパ球増殖性疾患(EBV-LPD)の1例2007

    • Author(s)
      榎本 泰典
    • Organizer
      第96回日本病理学会総会
    • Place of Presentation
      大阪市
    • Year and Date
      2007-03-14
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] Diagnostics of Methotrexate(MTX)-associated lymphoproliferative disorders2007

    • Author(s)
      Nakamine H, et. al.
    • Organizer
      Jpn Soc Pathol, 96th annual meeting
    • Place of Presentation
      Osaka City
    • Year and Date
      2007-03-14
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] EBV-associated lymphoproliferative disorder with plasmacytoid lymphocytosis.2007

    • Author(s)
      Enomoto Y, et. al.
    • Organizer
      Jpn Soc Pathol 96th annual meeting
    • Place of Presentation
      Osaka City
    • Year and Date
      2007-03-14
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Book] イラストとエッセンス, わかる病理学(小西 登, 編)「造血器疾患」2007

    • Author(s)
      中峯 寛和
    • Publisher
      恒心社出版(さいたま市).,pp120-137.,,(平成19年10月)
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary

URL: 

Published: 2006-04-01   Modified: 2016-04-21  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi