Analysis of telomere metabolism according to each chromosome in living patients with Werner syndrome using the FISH method
| Project/Area Number |
18590354
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Human pathology
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| Research Institution | Tokyo Metropolitan Institute of Gerontology |
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Principal Investigator |
NAKAMURA Ken-ichi Tokyo Metropolitan Institute of Gerontology, Tokyo Metropolitan Foundation for Research on Aging and Promotion of Human Welfare, Tokyo Metropolit, Rsearch Scientist (60159069)
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| Co-Investigator(Kenkyū-buntansha) |
TAKUBO Kaiyo Tokyo Metropolitan Institute of Gerontology, Team Leader (00154956)
IZUMIYAMA Naotaka Tokyo Metropolitan Institute of Gerontology, Research Assistant (10158751)
ISHII Akio Tokyo Metropolitan Institute of Gerontology, Research Assistant (60167244)
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| Project Period (FY) |
2006 – 2007
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| Project Status |
Completed (Fiscal Year 2007)
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| Budget Amount *help |
¥4,020,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥420,000)
Fiscal Year 2007: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2006: ¥2,200,000 (Direct Cost: ¥2,200,000)
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| Keywords | FISH / Telomere / Werner's syndrome / 染色体 |
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| Research Abstract |
We studied telomere metabolism and telomere length in fibroblasts from patients with Werner syndrome (WS) and normal control individuals by Q-FISH using metaphase spreads and tissue sections. We collected tissue samples from 7 cases of Werner syndrome Two of 9 tissue samples were obtained from the same patients on different dates. Primary cultured fibroblasts were also collected from 6 cases.
1. Karyotype analysis was completed for fibroblasts from 6 cases of WS.
2. A high frequency of translocation was associated with specific chromosomes.
3. Mean telomere lengths for fibroblasts in 3 of the 6 WS cases were 10.1 kbp, 5.0 kbp, and 6.1 kbp, respectively.
4. Telomere length of the chromosome bearing the WS gene was not significantly shorter than that for other chromosomes in 3 cases of WS.
5. We measured telomere lengths of lymphocytes from patients with other progeria syndromes (Down's syndrome and 18 trisomy, n = 21) , and telomeres of trisomy chromosomes were not significantly shorter than those of other chromosomes.
6. Telomere length measurement in the epidermis by Q-FISH method was prevented from obtaining constant date by cytoplasmic melanin and melanosomes. After digestion of melanin, we were able to obtain good results of telomere measurement.
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Report
(3 results)
Research Products
(20 results)
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[Journal Article] Telomere length variations in 6 mucosal cell types of gastric tissue observed using novel Q-FISH method2007
Author(s)
Aida J, Izumiyama-Shimomura N, Nakamura K, Ishii A, Ishikawa N, Honma N, Kurabayashi R, Kammori M, Poon SSS, Arai T, Takubo K
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Journal Title
Human Pathology (in press)
Related Report
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[Journal Article] Specific subtelomere loss on chromosome der(11)t(3;11)(q23;q23)x2 in anaplastic thyroid cancer cell line OCUT-1.2006
Author(s)
Kammori M, Onoda N, Nakamura K, Izumiyama N, Ogisawa K, Kurabayashi R, Ogawa T, Miura Y, Kaminishi M, Poon S, Takubo K
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Journal Title
Int J Mol Med 18・1
Pages: 9-16
Related Report
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[Journal Article] Telomere shortening with aging in the human pancreas.2006
Author(s)
Ishii A, Nakamura K, Kishimoto H, Honma N, Aida J, Sawabe M, Arai T, Fujiwara M, Takeuchi F, Kato M, Oshimura M, Izumiyama N, Takaubo K
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Journal Title
Exp Gerontol 41・9
Pages: 882-6
Related Report
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