| Project/Area Number |
18590354
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Human pathology
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| Research Institution | Tokyo Metropolitan Institute of Gerontology |
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Principal Investigator |
NAKAMURA Ken-ichi Tokyo Metropolitan Institute of Gerontology, Tokyo Metropolitan Foundation for Research on Aging and Promotion of Human Welfare, Tokyo Metropolit, Rsearch Scientist (60159069)
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| Co-Investigator(Kenkyū-buntansha) |
TAKUBO Kaiyo Tokyo Metropolitan Institute of Gerontology, Team Leader (00154956)
IZUMIYAMA Naotaka Tokyo Metropolitan Institute of Gerontology, Research Assistant (10158751)
ISHII Akio Tokyo Metropolitan Institute of Gerontology, Research Assistant (60167244)
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| Project Period (FY) |
2006 – 2007
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| Project Status |
Completed (Fiscal Year 2007)
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| Budget Amount *help |
¥4,020,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥420,000)
Fiscal Year 2007: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2006: ¥2,200,000 (Direct Cost: ¥2,200,000)
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| Keywords | FISH / Telomere / Werner's syndrome / 染色体 |
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| Research Abstract |
We studied telomere metabolism and telomere length in fibroblasts from patients with Werner syndrome (WS) and normal control individuals by Q-FISH using metaphase spreads and tissue sections. We collected tissue samples from 7 cases of Werner syndrome Two of 9 tissue samples were obtained from the same patients on different dates. Primary cultured fibroblasts were also collected from 6 cases.
1. Karyotype analysis was completed for fibroblasts from 6 cases of WS.
2. A high frequency of translocation was associated with specific chromosomes.
3. Mean telomere lengths for fibroblasts in 3 of the 6 WS cases were 10.1 kbp, 5.0 kbp, and 6.1 kbp, respectively.
4. Telomere length of the chromosome bearing the WS gene was not significantly shorter than that for other chromosomes in 3 cases of WS.
5. We measured telomere lengths of lymphocytes from patients with other progeria syndromes (Down's syndrome and 18 trisomy, n = 21) , and telomeres of trisomy chromosomes were not significantly shorter than those of other chromosomes.
6. Telomere length measurement in the epidermis by Q-FISH method was prevented from obtaining constant date by cytoplasmic melanin and melanosomes. After digestion of melanin, we were able to obtain good results of telomere measurement.
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