Molecular basis for renal regeneration : New approach using a nephron-specific HGF-knockout mice

• Project/Area Number: 18590369
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Experimental pathology
• Research Institution: Osaka University
• Principal Investigator: MIZUNO Shinya Osaka University, Graduate School of Medicine, Assistant Professor (10219644)
• Co-Investigator(Kenkyū-buntansha): NAKAMURA Toshikazu Osaka University, Graduate School of Medicine, Professor (00049397)
• Project Period (FY): 2006 – 2007
• Project Status: Completed (Fiscal Year 2007)
• Budget Amount: ¥3,160,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥360,000); Fiscal Year 2007: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2006: ¥1,600,000 (Direct Cost: ¥1,600,000)
• Keywords: HGF / c-Met / Nephron / Renal regeneration / Renal diseases / Molecular mechanism / Renal protection / Knockout mice / Met / 組織修復 / 腎保護効果

Research Abstract

It was suggested that HGF may be a key physiological regulator for tissue reconstruction after acute renal injuries, but there was little direct evidence for supporting this hypothesis. Herein we provide evidence to show that endohenous HGF/c-Met axis plays an important role for inducing renal regeneration in mice after acute renal injury, using anti-HGF antibody or HGF gene targeting techniques, as follows :
(1) Renal tubular proliferation became evident in mice at 48-hr after renal ischemia and reperfusion. When anti-HGF IgG was administered into mice, tubular regeneration was inhibited, associated with the enhancement of bone marrow engraftment. These findings indicated the importace of endogenous HGF to elicit tubular cell proliferation, rather than stem cell involvement under renal ischemia.
(2) Is is known that 70%-partial hepatectomy attenuated the onset of acute renal failure after muscular damages in rats. Anti-HGF IgG diminished the protection of renal injury by 70%-hepatectomy, hence indicating the pivotal roles of endogenous HGF, induced by the loss in hepatic lobes, for protection of renal damage after crush syndorome.
(3) We prepared the podocyte-specific deletion of HGF gene, using a Cre-LoxP system. There was no significant lesions in the renal tissues of the HGF-deficient mice during a developmental stage. However, HGF-deficient mice seemed to be more sensitive to a chemical drug, which causes proteinuria.

Research Products

• [Journal Article] HGF as a renotrophic and anti-fibrotic regulator in chronic renal disease. (2008)
  • Author(s): Mizuno S. and Nakamura T.
  • Journal Title: Frontier in Bioscience 13 Pages: 7072-7086
  • Description: 「研究成果報告書概要(和文)」より
  • Peer Reviewed
• [Journal Article] Inhibition of tubular cell proliferation by neutralizing endogenous HGF leads to renal hypoxia and bone marrow-derived cell engraftment in acute renal failure. (2008)
  • Author(s): Ohnishi H., et. al.
  • Journal Title: Am. J. Physiol 294
  • Description: 「研究成果報告書概要(和文)」より
  • Peer Reviewed
• [Journal Article] HGF as a renotrophic and anti-fibrotic regulator in chronic renal disease (2008)
  • Author(s): Mizuno, S, Matsumoto, K, Nakamura, T
  • Journal Title: Frontier in Bioscience 13 Pages: 7072-7086
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Inhibition of tubular cell proliferation by neutralizing endogenos HGF leads to renal hypoxia and bone marrow-derived cell engraftment in acute renal failure of mice (2008)
  • Author(s): Ohnishi, H, Mizuno, S, Nakamura, T
  • Journal Title: Am J Physiol 294 Pages: 326-335
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] NK4 gene therapy targeting HGF-Met and angiogenesis. (2008)
  • Author(s): Matsumoto K. and Nakamura T.
  • Journal Title: Front Biosci. 13 Pages: 1943-1951
  • Peer Reviewed
• [Journal Article] Hepatocyte Growth Factor and Met in Tumor Biology and Therapeutic Approach with NK4. (2008)
  • Author(s): Matsumoto K, et. al.
  • Journal Title: Proteomics (In press)
  • Peer Reviewed
• [Journal Article] Generation of engineered recombinant hepatocyte growth factor cleaved and activated by Generase I. (2008)
  • Author(s): Hayata D., et. al.
  • Journal Title: J. Biotechnol. 133 Pages: 478-85
  • Peer Reviewed
• [Journal Article] Regulation of cell migration and cytokine production by HGF-like protein (HLP)/macrophage-stimulating protein(MSP)in primary microglia. (2008)
  • Author(s): Suzuki Y., et. al.
  • Journal Title: Biomed. Res. (In press)
  • Peer Reviewed
• [Journal Article] Induction of hepatocyte growth factor by fucoidan and fucoidan-derived oligosaccharides. (2008)
  • Author(s): Fukuta K., et. al.
  • Journal Title: J. Pharm. Pharmacol. (In press)
  • Peer Reviewed
• [Journal Article] The functions and possible significance of Kremen as the gatekeeper of Wnt signaling in development and pathology. (2008)
  • Author(s): Nakamura T., et. al.
  • Journal Title: J. Cell. Mol. Med. (In press)
  • Peer Reviewed
• [Journal Article] Reduction of fibrosis in a rat model of non-alcoholic steatohepatitis cirrhosis by human HGF gene transfection using electroporation. (2008)
  • Author(s): Kiyama S., et. al.
  • Journal Title: J. Gastroenterol. Hepatol. (In press)
  • Peer Reviewed
• [Journal Article] Hepatocyte growth factor improves synaptic localization of the NMDA receptor and intracellular signaling after excitotoxic injury in cultured hippocampal neurons. (2008)
  • Author(s): Akita H., et. al.
  • Journal Title: J. Neurochem. (In press)
  • Peer Reviewed
• [Journal Article] Hepatocyte growth factor: A regenerative drug for acute hepatitis and liver cirrhosis. (2007)
  • Author(s): Mizuno S. and Nakamura T.
  • Journal Title: Regenerative Med. 2 Pages: 161-170
  • Description: 「研究成果報告書概要(和文)」より
  • Peer Reviewed
• [Journal Article] Attenuation of glycerol-induced acute kidney injury by previous partial hepatectomy: Role of hepatocyte growth factor/c-met axis in tubular protection. (2007)
  • Author(s): Homsi E., et. al.
  • Journal Title: Nephron. Exp. Nephrol. 107
  • Description: 「研究成果報告書概要(和文)」より
  • Peer Reviewed
• [Journal Article] Growth factors temporally associate with airway responsiveness and inflammation in allergen-exposed mice. (2007)
  • Author(s): Ito W., et. al.
  • Journal Title: Int. Arch. Allergy Immunol. 148 Pages: 324-339
  • Description: 「研究成果報告書概要(和文)」より
  • Peer Reviewed
• [Journal Article] Hepatocyte growth factor prevents pulmonary ischemia-reperfusion injury in mice. (2007)
  • Author(s): Makiuchi A., et. al.
  • Journal Title: J. Heart Lung Transplant. 26 Pages: 935-943
  • Description: 「研究成果報告書概要(和文)」より
  • Peer Reviewed
• [Journal Article] Antinecrotic and antiapoptotic effects of hepatocyte growth factor on cholestatic hepatitis in a mouse model of bile-obstructive diseases. (2007)
  • Author(s): Li Z., et. al.
  • Journal Title: Am. J. Physiol. 292
  • Description: 「研究成果報告書概要(和文)」より
  • Peer Reviewed
• [Journal Article] Hepatocyte growth factor : A regenerative drug for acute hepatitis and liver cirrhosis (2007)
  • Author(s): Mizuno, S, Nakamura, T
  • Journal Title: Regenerative Medicine 2 Pages: 161-170
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Attenuation of glycerol-induced acute kidney injury by previous partial hepatectomy : Role of hepatocyte growth factor/c-met axis in tubular protection (2007)
  • Author(s): Homsi, E, Janino, P, Biswas, SW, Mizuno, S, Nakamura, T, Lopes, de, Faria, JB
  • Journal Title: Nephron Exp 107 Pages: 95-106
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Growth factors temporally associate with airway responsiveness and inflammation in allergen-exposed mice (2007)
  • Author(s): Ito, W, Tanimoto, M, Ono, K, Mizuno, S, Yoshida, A, Koga, H, Fuchimoto, Y, Kondo, N, Tanimoto, Y, Kiura, K, Matsumoto, K., Kataoka, M, Nakamura, T, Gelfand ,EW, Kanehiro, A
  • Journal Title: Int Arch Allergy Immunol 148 Pages: 324-339
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Hepatocyte growth factor prevents pulmonary ischemia-reperfusion injury in mice (2007)
  • Author(s): Makiuchi, A, Yamaura, K, Mizuno, S, Matsumoto, K, Nakamura, T, Amano, J, Ito, K
  • Journal Title: J Heart Lung Transplant 26 Pages: 935-943
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Anti-necrotic and anti-apoptotic effects of HGF on cholestatic hepatitis in a mouse model of bile congestive diseases (2007)
  • Author(s): Li, Z, Mizuno, S, Nakamura, T
  • Journal Title: Am J Physiol 292 Pages: 639-646
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Hepatocyte growth factor:A regenerative drug for acute hepatitis and liver cirrhosis. (2007)
  • Author(s): Mizuno S. and Nakamura T.
  • Journal Title: Regenerative Med. 2 Pages: 161-170
  • Peer Reviewed
• [Journal Article] Attenuation of glycerol-induced acute kidney injury by previous partial hepatectomy:Role of hepatocyte growth factor/c-met axis in tubular protection. (2007)
  • Author(s): Homsi E., et. al.
  • Journal Title: Nephron. Exp. Nephrol. 107
  • Peer Reviewed
• [Journal Article] Hepatocyte growth factor(HGF)promotes oligodendrocyte progenetor cell proliferation and inhibits its differentiaion during postnatal development in the rat. (2007)
  • Author(s): Ohya W., et. al.
  • Journal Title: Brain Res. 1147 Pages: 51-65
  • Peer Reviewed
• [Journal Article] Hepatocyte growth factor(HGF)attenuates gliosis and motoneuronal degeneration in the brainstem motor nuclei of a transgenic mouse model of ALS. (2007)
  • Author(s): Kadoyama K., et. al.
  • Journal Title: Neurosci. Res. 59 Pages: 446-456
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• [Journal Article] Inhibition of colon cancer growth and metastasis by NK4 gene repetitive delivery in mice. (2007)
  • Author(s): Wen J., et. al.
  • Journal Title: Biochem, Biophys. Res. Commun. 358 Pages: 117-123
  • Peer Reviewed
• [Journal Article] Stimulation of hepatocyte growth factor production by heparin-derived oligosaccharides. (2007)
  • Author(s): Sakiyama R., et. al.
  • Journal Title: J. Biochem. 141 Pages: 653-660
  • Peer Reviewed
• [Journal Article] A genomic analysis of adult T-cell leukemia. (2007)
  • Author(s): Choi Y. L., et. al.
  • Journal Title: Oncogene 26 Pages: 1245-1255
  • Peer Reviewed
• [Journal Article] Transcription of the MAT2A gene, coding for methionine adenosyltransferase, is up-regulated by E2F and Sp1 at a chromatin level during proliferation of liver cells. (2007)
  • Author(s): Rodriguez L, et. al.
  • Journal Title: Biochem. Cell Biol. 39 Pages: 842-850
  • Peer Reviewed
• [Journal Article] Hepatocyte growth factor promoted endogenous repair and functional recovery after spinal cord injury. (2007)
  • Author(s): Kitamura K, et. al.
  • Journal Title: J. Neurosci. Res. 85 Pages: 2332-2342
  • Peer Reviewed
• [Journal Article] Targeted delivery of NK4 to multiple lung tumors by bone marrow-derived mesenchymal stem cells. (2007)
  • Author(s): Kanehira M., et. al.
  • Journal Title: Cancer Gene Ther. 14 Pages: 894-903
  • Peer Reviewed
• [Journal Article] Intrathecal delivery of hepatocyte growth factor from amyotrophic lateral sclerosis onset suppresses disease progression in rat amyotrophic lateral sclerosis model. (2007)
  • Author(s): Ishigaki A., et. al.
  • Journal Title: J. Neuropathol. Exp. Neurol. 66 Pages: 1037-1044
  • Peer Reviewed
• [Journal Article] Improvement of survival of skin flaps by combined gene transfer of hepatocyte growth factor and prostacyclin synthase. (2007)
  • Author(s): Nakagawa A., et. al.
  • Journal Title: J. Gene. Med. 9 Pages: 1087-1094
  • Peer Reviewed
• [Journal Article] Hepatocyte growth factor promotes the number of PSD-95 clusters in young hippocampal neurons. (2007)
  • Author(s): Nakano M., et. al.
  • Journal Title: Exp. Neurol. 207 Pages: 195-202
  • Peer Reviewed
• [Journal Article] NK4, an antagonist of hepatocyte growth factor(HGF), inhibits growth of multiple myeloma cells: Molecular targeting of angiogenic growth factor. (2007)
  • Author(s): Du W., et. al.
  • Journal Title: Blood 109 Pages: 3042-3049
  • Peer Reviewed
• [Journal Article] Hepatocyte growth factor : A regenerative drug for acute hepatitis and liver cirrhosis (Review). (2007)
  • Author(s): Mizuno S. et al.
  • Journal Title: Regenerative Med. (In press)
• [Journal Article] Anti-necrotic and anti-apopyoyic effects of hepatocyte growth factor on cholestatic hepatitis in a mouse model of bile-obistructive diseases. (2007)
  • Author(s): Li Z.et al.
  • Journal Title: Am. J. Physlol. (In press)
• [Journal Article] Hepatocyte growth factor (HGF) promotes oligodendrocyte progenetor cell proliferation and inhibits its differentiaion during postnatal development in the rat. (2007)
  • Author(s): Ohya W.et al.
  • Journal Title: Brain Res. (In press)
• [Journal Article] NK4, an antagonist of hepatocyte growth factor (HGF), inhibits growth of multiple myeloma cells in vivo; molecular targeting of angiogenic growth factor. (2007)
  • Author(s): Du W.et al.
  • Journal Title: Blood (In press)
• [Journal Article] A genomic analysis of adult T-cell leukemia. (2007)
  • Author(s): Choi W.L.et al.
  • Journal Title: Oncogene (In press)
• [Journal Article] Lung tissue engineering technique with adipose tissue-derived stromal cells improves surgical outcomes for pulmonary emphysema. (2006)
  • Author(s): Shigemura N., et. al.
  • Journal Title: Am J Respir Crit Care Med 174 Pages: 1199-1205
  • Description: 「研究成果報告書概要(和文)」より
  • Peer Reviewed
• [Journal Article] Autologous transplantation of adipose tissue-derived stromal cells ameliorates pulmonary emphysema. (2006)
  • Author(s): Shigemura N., et. al.
  • Journal Title: Am J Transplant 6 Pages: 2592-2600
  • Description: 「研究成果報告書概要(和文)」より
  • Peer Reviewed
• [Journal Article] Prevention of onset of Parkinson's disease by in vivo gene transfer of human hepatocyte growth factor in rodent model: a model of gene therapy for Parkinson's disease. (2006)
  • Author(s): Koike H., et. al.
  • Journal Title: Gene Therapy 13 Pages: 1639-1644
  • Description: 「研究成果報告書概要(和文)」より
  • Peer Reviewed
• [Journal Article] Endothelial progenitor cell transplantation improves the survival following liver injury in mice. (2006)
  • Author(s): Taniguchi E., et. al.
  • Journal Title: Gastroenterology 130 Pages: 521-531
  • Description: 「研究成果報告書概要(和文)」より
  • Peer Reviewed
• [Journal Article] The loss of local HGF, an endogenous gastrophic factor, leads to mucosal injuries in the stomach of mice. (2006)
  • Author(s): Nakahira R., et. al.
  • Journal Title: Biochem Biophys Res Communs 341 Pages: 897-903
  • Description: 「研究成果報告書概要(和文)」より
  • Peer Reviewed
• [Journal Article] Lung tissue engineering technique with adipose tissue-derived stromal cells improves surgical outcomes for pulmonary emphysema (2006)
  • Author(s): Shigemura, N, Okumura, M, Mizuno, S, Imanishi, Y, Matsuyama, A, Shiono, H, Nakamura, T, Sawa, Y
  • Journal Title: Am J Respir Crit Care Med 174 Pages: 1199-1205
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Autologous transplantation of adipose tissue-derived stromal cells ameliorates pulmonary emphysema (2006)
  • Author(s): Shigemura, N, Okumura, M, Mizuno, S, Imanishi, Y, Nakamura, T, Sawa, Y
  • Journal Title: Am J Transplant 6 Pages: 2592-2600
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Prevention of onset of Parkinson's disease by in vivo gene transfer of human hepatocyte growth factor in rodent model : a model of gene therapy for Parkinson's disease (2006)
  • Author(s): Koike, H, Ishida, A, Shimamura, M, Mizuno, S, Nakamura, T, Ogihara, T, Kaneda, Y, Morishita, R
  • Journal Title: Gene Therapy 13 Pages: 1639-1644
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Endothelial progenitor cell transplantation improves the survival following liver injury in mice (2006)
  • Author(s): Taniguchi, E, Kin, M, Torimura, T, Nakamura, T, Kumemura, H, Hanada, S, Hisamoto, T, Yoshida, T, Kawaguchi, T, Baba, S, Maeyama, M, Koga, H, Harada, M, Kumashiro, R, Ueno, T, Mizuno, S, Ikeda, H, Imaizumi, T, Murohara, T, Sata, M
  • Journal Title: Gastroenterology 130 Pages: 521-531
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] The loss of local HGF, an endogenous gastrophic factor, leads to mucosal injuries in the stomach of mice (2006)
  • Author(s): Nakahira, R, Mizuno, S, Yoshimine, T, Nakamura, T
  • Journal Title: Biochem Biophys Res Commons 341 Pages: 897-903
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Chapter 13. Hepatocyte growth factor : Physiological and therapeutic ligand to attenuate diabetic nephropathy (2006)
  • Author(s): Mizuno, S, Nakamura, T
  • Journal Title: Diabetic Kidney, (Editted by Mogensen CE and Cortes P) Pages: 225-253
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] VII. Renal Failure. Section of growth factor (2006)
  • Author(s): Mizuno, S, Nakamura, T
  • Journal Title: Growth factor and Regener. Med., (Eddited by Matsumoto K and Tabata T) (in Japanese) Pages: 219-229
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] V. 25. Molecular mechanisms for HGF-mediated regression of renal fibrosis (2006)
  • Author(s): Mizuno, S, Nakamura, T
  • Journal Title: Molecular Basis for renal disease, (Editted by Miyata T, et. al.) (in Japanese) Pages: 312-321
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] The loss of local HGF, an endogenenous gastrotrophic factor, lead to mucosal injuries in the stomach of mice. (2006)
  • Author(s): Nakahira R. et al.
  • Journal Title: Blochem. Blophys. Res. Commun. 341 Pages: 897-903
• [Journal Article] Prevention of onset of Parkinson's disease by in vivo gene transfer of human hepatocyte growth factor in rodent model : a model of gene therapy for Parkinson's disease. (2006)
  • Author(s): Koike H. et al.
  • Journal Title: Gene Therapy 13 Pages: 1639-1644
• [Journal Article] Autologous transplantation of adipose tissue-derived stromal cells ameliorates pulmonary emphysema. (2006)
  • Author(s): Shigemura N. et al.
  • Journal Title: Am. J. Transplant. 6 Pages: 2592-2600
• [Journal Article] Lung tissue engineering technique with adipose tissue-derived stromal cells improves surgical outcomes for pulmonary emphysema. (2006)
  • Author(s): Shigemura N. et al.
  • Journal Title: Am. J. Respir. Crit. Care Med. 174 Pages: 1199-1205
• [Journal Article] Contact inhibition of hepatocyte growth factor regulated by functional association of the c-Met/HGF receptor and LAR protein tyrosine phosphatase. (2006)
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• [Journal Article] Different acting regulation and sub-cellular localization of LIMK1 and LIMK2 during cell cycle transition. (2006)
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• [Journal Article] Possible role of scarenger receptor SRCL in the clearance of amyloid-beta in Alzheimer's disease. (2006)
  • Author(s): Nakamura K. et al.
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• [Journal Article] Hepatocyte growth factor and the Met system as a mediator in tumor-stromal interactions (Review). (2006)
  • Author(s): Matsumoto K. et al.
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• [Journal Article] Angiogenetic and anti-fibrotic actions of hepatocyte growth factor improve cardiac dysfunction in porcine ischemic cardiomyopathy. (2006)
  • Author(s): Azuma J. et al.
  • Journal Title: Gene Therapy 13 Pages: 1206-1213
• [Journal Article] Novel therapeutic strategy for stroke in rats by bone marrow stromal cells and ex vivo HGF gene transfer with HSV-1 vector. (2006)
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  • Journal Title: J. Cereb. Blood Flow Met. 26 Pages: 1176-1188
• [Journal Article] Prevention of apoptosis-inducing factor translocation is a possible mechanism for protective effects of hepatocyte growth factor against neuronal cell death in the hippocampous after transient forebrain ischemia. (2006)
  • Author(s): Niimura M. et al.
  • Journal Title: J. Cereb. Blood Flow Met. 26 Pages: 1354-1365
• [Journal Article] Gene transfer of hepatocyte growth factor improves learning and memory in the chronic stage of cerebral infaction. (2006)
  • Author(s): Shimamura M. et al.
  • Journal Title: Hypertenslon 47 Pages: 742-751
• [Journal Article] Enhanced suppression of tumor growth using a combination of NK4 plasmid DNA-PEG engrafted cationized dextran complex and ultrasound irradiation. (2006)
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  • Journal Title: Cancer Gene Ther. 13 Pages: 479-489
• [Journal Article] Peritumoral injection of adenovirus vector expressing NK4 combined with gemcitabine treatment suppresses growth and metastasis of human pancreatic cancer cells implanted orthotopically in nude mice and prolongs survival. (2006)
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  • Journal Title: Cancer Gene Ther. 13 Pages: 520-529
• [Journal Article] Intraventricular administration of hepatocyte growth factor treats mouse communicating hydrocephalus induced by human recombinant TGF-betal. (2006)
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  • Journal Title: Neurobiol. Disease 21 Pages: 576-578
• [Journal Article] Effect of hepatocyte growth factor on phosphorylation of extracellular signal-regulated kinase and hippocampal cell death in rats with transient forebrain ischemia. (2006)
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  • Journal Title: Eur. J. Pharmacol 535 Pages: 114-124
• [Journal Article] Adenovial gene transfer of hepatocyte growth factor prevents death of injured adult motoneurons after peripheral never avulsion. (2006)
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  • Journal Title: Brain Res. 1111 Pages: 187-195
• [Journal Article] Involvement of hepayocyte growth factor in the development of bone metastasis of a mouse mammary cancer cell line, BALB/c-MC. (2006)
  • Author(s): Ono K.et al.
  • Journal Title: Bone 39 Pages: 27-34
• [Journal Article] Hepatocyte growth factor in mouse soleus muscle increases with reloading after unloading. (2006)
  • Author(s): Tanaka S. et al.
  • Journal Title: J. Phys. Ther. Sci. 18 Pages: 33-41
• [Journal Article] The protective effect of hepatocyte growth factor against cell death in the hippocampus after transient forebrain ischemia is related to the improvement of apurinic/apyrimidinic endonuclease/redox factor-1 level and inhibition of NADPH oxidase activity. (2006)
  • Author(s): Niimura M. et al.
  • Journal Title: Neurosci Lett. 407 Pages: 136-140
• [Journal Article] Hepatocyte growth factor attenuates cerebral ischemia-induced increase in permeability of blood-brain barrier and decreases in expression of tight junctional proteins in cerebral vessels. (2006)
  • Author(s): Date l.et al.
  • Journal Title: Neurosci Lett. 407 Pages: 141-145
• [Journal Article] Liver targeting of plasmid DNA with a cationized pullulan for tumor suppression. (2006)
  • Author(s): Jo J.et al.
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• [Journal Article] Blockage of HGF/c-Met system by gene therapy (adenovirus-mediated NK4 gene) suppresses hepatocellular carcinoma in mice. (2006)
  • Author(s): Son G.et al.
  • Journal Title: J. Hepatol. 45 Pages: 688-695
• [Journal Article] Therapeutic effect of transplanting HGF-treated bone marrow mesenchymal cells into CC14-injured rats. (2006)
  • Author(s): Oyagi.et al.
  • Journal Title: J. Hepatol. 44 Pages: 742-748
• [Journal Article] Preclinical study of a "tailor-made" combination of NK4-expressing gene therapy and gefitinib (ZD1839, Iressatrade mark) for disseminated peritoneal scirrhous gastric cancer. (2006)
  • Author(s): Namiki Y. et al.
  • Journal Title: Int. J. Cancer 118 Pages: 1545-1555
• [Presentation] HGFによるマクロファージでのヘムオキシゲナーゼ(HO-1)産生亢進を介した敗血症マウスでの肝炎発症阻止 (2008)
  • Author(s): 神元 幸, ら
  • Organizer: 第7回日本再生医療学会総会
  • Place of Presentation: 名古屋
  • Year and Date: 2008-03-14
  • Description: 「研究成果報告書概要(和文)」より
• [Presentation] Induction of HO-1 in macropahes by HGF contributes to improving hepatitis in a mouse model of sepsis (2008)
  • Author(s): Kamimoto, M, Mizuno, S, Nakamura, T
  • Organizer: 7th Congress of the Japanese Society for Regenerative Medicine
  • Place of Presentation: NAGOYA
  • Year and Date: 2008-03-14
  • Description: 「研究成果報告書概要(欧文)」より
• [Presentation] HGFによるマクロファージでのへムオキシゲナーゼ(HO-1)産生亢進を介した敗血症マウスでの肝炎発症阻止 (2008)
  • Author(s): 神元 幸, ら
  • Organizer: 第7回日本再生医療学会総会
  • Place of Presentation: 名古屋
  • Year and Date: 2008-03-14
• [Presentation] HGFによる敗血症モデルでの急性肝炎発症阻止とその分子機構 (2007)
  • Author(s): 神元 幸, ら
  • Organizer: 第80回日本生化学会大会・第30回日本分子生物学会年会合同大会
  • Place of Presentation: 横浜
  • Year and Date: 2007-12-14
  • Description: 「研究成果報告書概要(和文)」より
• [Presentation] Molecular basis for treatment of acute hepatitis by HGF in a mouse model of experimental sepsis (2007)
  • Author(s): Kamimoto, M, Mizuno, S, Nakamura, T
  • Organizer: 80th Congress of the Japanese Biochemical Society
  • Place of Presentation: YOKOHAMA
  • Year and Date: 2007-12-14
  • Description: 「研究成果報告書概要(欧文)」より
• [Presentation] NK4による血管新生阻害機構:NK4による接着斑形成とフィブロネクチン構築の阻害 (2007)
  • Author(s): 酒井 克也, ら
  • Organizer: 第80回日本生化学会大会・第30回日本分子生物学会年会合同大会
  • Place of Presentation: 横浜
  • Year and Date: 2007-12-14
• [Presentation] オリゴデンドロサイト発生過程におけるHGF-c-Metのもつ生理活性 (2007)
  • Author(s): 大谷 若菜, ら
  • Organizer: 第80回日本生化学会大会・第30回日本分子生物学会年会合同大会
  • Place of Presentation: 横浜
  • Year and Date: 2007-12-14
• [Presentation] JXM領域欠損型c-Met/HGFレセプターマウスでは、肝臓発生はおこったが、筋肉発育不全が認められた (2007)
  • Author(s): 中山 瑞穂, ら
  • Organizer: 第80回日本生化学会大会・第30回日本分子生物学会年会合同大会
  • Place of Presentation: 横浜
  • Year and Date: 2007-12-13
• [Presentation] トリプトファン2,3-ジオキシゲナーゼ(TDO)ノックアウトは、情動行動と神経新生を修飾する (2007)
  • Author(s): 金井 将昭, ら
  • Organizer: 第80回日本生化学会大会・第30回日本分子生物学会年会合同大会
  • Place of Presentation: 横浜
  • Year and Date: 2007-12-11
  • Description: 「研究成果報告書概要(和文)」より
• [Presentation] 内因性HGFの機能阻害を介した尿細管上皮増殖抑制と骨髄幹細胞動員亢進 (2007)
  • Author(s): 大西 浩之, ら
  • Organizer: 第80回日本生化学会大会・第30回日本分子生物学会年会合同大会
  • Place of Presentation: 横浜
  • Year and Date: 2007-12-11
  • Description: 「研究成果報告書概要(和文)」より
• [Presentation] Disruption of tryptophan 2,3-dioxygenase(TDO) modulates emotional behavior and adult neurogenesis (2007)
  • Author(s): Kanai, M, Funakoshi, H, Mizuno, S., Nakamura, T., et. al.
  • Organizer: 80th Congress of the Japanese Biochemical Society
  • Place of Presentation: YOKOHAMA
  • Year and Date: 2007-12-11
  • Description: 「研究成果報告書概要(欧文)」より
• [Presentation] Inhibition of renal tubular cell proliferation and acceleration of bone marrow-derived cell engraftment by neutralizing endogenous HGF in acute renal failure (2007)
  • Author(s): Ohnishi, H, Mizuno, S, Nakamura, T
  • Organizer: 80th Congress of the Japanese Biochemical Society
  • Place of Presentation: YOKOHAMA
  • Year and Date: 2007-12-11
  • Description: 「研究成果報告書概要(欧文)」より
• [Presentation] LARチロシンホスファターゼのプロセシングを介した細胞密度依存的な肝細胞増殖抑制機構 (2007)
  • Author(s): 町出 充, ら
  • Organizer: 第80回日本生化学会大会・第30回日本分子生物学会年会合同大会
  • Place of Presentation: 横浜
  • Year and Date: 2007-12-11
• [Presentation] トリプトファン2,3-ジオキシゲナーゼ(TDO)ノックアウトは、情動行動と神経新生を修飾する (2007)
  • Author(s): 金井 将昭, ら
  • Organizer: 第80回本生化学会大会・第30回日本分子生物学会年会合同大会
  • Place of Presentation: 横浜
  • Year and Date: 2007-12-11
• [Presentation] 内因性HGFの機能阻害を介した尿細管上皮増殖抑制と骨髄幹細胞動員充進 (2007)
  • Author(s): 大西 浩之, ら
  • Organizer: 第80回日本生化学会大会・第30回日本分子生物学会年会合同大会
  • Place of Presentation: 横浜
  • Year and Date: 2007-12-11
• [Presentation] NK4による細胞外フィブロネクチン構築と接着斑形成の阻害. (2007)
  • Author(s): 酒井 克也, ら
  • Organizer: 第66回日本癌学会総会
  • Place of Presentation: 横浜
  • Year and Date: 2007-10-05
• [Presentation] NK4は癌細胞-血管内皮細胞接着を阻害することでCT26肺転移を抑制する. (2007)
  • Author(s): 窪田 健, ら
  • Organizer: 第66回日本癌学会総会
  • Place of Presentation: 横浜
  • Year and Date: 2007-10-05
• [Presentation] Hepatocyte growth factor (HGF) as a trophic factor for oligodendrocyte progenitor cells (OPCs) during postnatal development in the rat. (2007)
  • Author(s): Ohya W., et. al.
  • Organizer: The 30th Annual Meeting of Japan Neuroscience Society (JNS)
  • Place of Presentation: Yokohama
  • Year and Date: 2007-09-11
• [Presentation] Gene transfer of hepatocyte growth factor (HGF) attenuates behavioral and neuropathological abnormalities in a transgenic mouse model of Huntington's disease. (2007)
  • Author(s): Funakoshi H., et. al.
  • Organizer: The 30th Annual Meeting of Japan Neuroscience Society (JNS)
  • Place of Presentation: Yokohama
  • Year and Date: 2007-09-10
• [Presentation] Reciprocal phosphorylation of tyrosine and serine residues of c-Met/HGF receptor in motoneurons implicates HGF-dependent disease retardation of a transgenic mouse model of ALS. (2007)
  • Author(s): Kadoyama K., et. al.
  • Organizer: The 30th Annual Meeting of Japan Neuroscience Society (JNS)
  • Place of Presentation: Yokohama
  • Year and Date: 2007-09-10
• [Presentation] Alteration of Systemic/Brain Tryptophan Metabolism and Modulation of Anxiety-related Behavior in Tryptophan 2,3-Dioxygenase (TDO)-Deficient Mice (2007)
  • Author(s): Kanai M., et. al.
  • Organizer: 30th Annual Meeting of Japan Neuroscience Society
  • Place of Presentation: Yokohama
  • Year and Date: 2007-09-10
• [Presentation] Modulation of Neurogenesis and Alteration of Emotional Behavior in Tryptophan 2,3-Dioxygenase-Deficient Mice (2007)
  • Author(s): Kanai, M., Kanai, M, Funakoshi, H, Mizuno, S, Nakamura, T., et. al.
  • Organizer: 27th Sapporo Cancer Seminar Intemational Symposium
  • Place of Presentation: SAPPORO
  • Year and Date: 2007-07-13
  • Description: 「研究成果報告書概要(欧文)」より
• [Presentation] Modulation of Neurogenesis and Alteration of Emotional Behavior in Tryptophan 2,3-Dioxygenase-Deficient Mice (2007)
  • Author(s): Kanai M., et. al.
  • Organizer: 27th Sapporo Cancer Seminar International Symposium
  • Place of Presentation: Sapporo
  • Year and Date: 2007-07-13
• [Presentation] Inhibition of tumor metastasis and angiogenesis by NK4, bifunctional inhibitor of HGF-Met and angiogenesis (2007)
  • Author(s): Matsumoto K., et. al.
  • Organizer: 27th Sapporo Cancer Seminar International Symposium
  • Place of Presentation: Sapporo
  • Year and Date: 2007-07-12
  • Description: 「研究成果報告書概要(和文)」より
• [Presentation] HGFによる腎修復機構:尿細管上皮再生に依存し、造血幹細胞動員に依存しない (2007)
  • Author(s): 大西浩之, ら
  • Organizer: 第6回日本再生医療学会総会
  • Place of Presentation: 横浜
  • Year and Date: 2007-03-13
  • Description: 「研究成果報告書概要(和文)」より
• [Presentation] Molecular basis for HGF-mediated renal repair : Molecular basis for renal tubular cell proliferation, but not recruitment of bone marrow-derived progenitor cells. (2007)
  • Author(s): Ohnishi, H, Mizuno, S, Nakamura, T
  • Organizer: 6th Congress of the Japanese Society for Regenerative Medicine
  • Place of Presentation: YOKOHAMA
  • Year and Date: 2007-03-13
  • Description: 「研究成果報告書概要(欧文)」より
• [Presentation] HGFによる閉塞性胆管疾患の進展阻止とその作用起点の解析 (2007)
  • Author(s): 水野信哉, ら
  • Organizer: 第6回日本再生医療学会総会
  • Place of Presentation: 横浜
  • Description: 「研究成果報告書概要(和文)」より
• [Presentation] Possible mechanisms underlying HGF-mediated protection of acute hepatisis in a mouse model of Chlectasis, induced by toal bil duct ligation (2007)
  • Author(s): Mizuno, S, Nakamura, T
  • Organizer: 6th Congress of the Japanese Society for Regenerative Medicine
  • Place of Presentation: YOKOHAMA
  • Description: 「研究成果報告書概要(欧文)」より
• [Book] Electroporation Protocols (2008)
  • Author(s): Komamura K., et. al.
  • Publisher: Springer
• [Book] Diabetic Nephropathy Book (2007)
  • Author(s): Mizuno S., et. al.
  • Publisher: The Humana Press
• [Book] Gene Therapy (2007)
  • Author(s): Matsumoto K., et. al.
  • Publisher: Medical View Co.
• [Book] Clinical Neuroscience (2007)
  • Author(s): 船越 洋、ほか.
  • Publisher: 中外医学社
• [Book] Brain and Nerve-神経研究の進歩 (2007)
  • Author(s): 船越 洋、ほか.
  • Publisher: 医学書院
• [Book] 難病と在宅ケア (2007)
  • Author(s): 船越 洋、ほか.
  • Publisher: 日本プランニングセンター
• [Book] 神経変性疾患のサイエンスThe Frontiers in Medical Scicnces (2007)
  • Author(s): 船越 洋、ほか.
  • Publisher: 南山堂
• [Book] Diabetic Nephropathy (2006)
  • Author(s): Mizuno S., et. al.
  • Publisher: The Humana Press
  • Description: 「研究成果報告書概要(和文)」より
• [Book] 細胞増殖因子と再生医療 (2006)
  • Author(s): 水野信哉、中村敏一
  • Publisher: メディカルレビュー社
  • Description: 「研究成果報告書概要(和文)」より
• [Book] 分子腎臓病学-分子生物学的アプローチと分子病態生理学- (2006)
  • Author(s): 水野信哉、中村敏一
  • Publisher: 日本臨床社
  • Description: 「研究成果報告書概要(和文)」より
• [Book] Diabetic Kidney (2006)
  • Author(s): Mizuno S, Nakamura T
  • Total Pages: 29
  • Publisher: Humana Press, Totawa, NJ, USA
• [Book] 細胞増殖因子と再生医療 (2006)
  • Author(s): 水野信哉, 中村敏一
  • Total Pages: 11
  • Publisher: メディカルレビュー社(東京)
• [Book] 分子腎臓病学 : 分子生物学的アプローチと分子病態生理学 (2006)
  • Author(s): 水野信哉, 中村敏一
  • Total Pages: 10
  • Publisher: 日本臨床社(東京)
• [Book] 細胞増殖因子と再生医療 (2006)
  • Author(s): 松本邦夫, 中村敏一
  • Total Pages: 8
  • Publisher: メディカルレビュー社(東京)